<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>1870-3453</journal-id>
<journal-title><![CDATA[Revista mexicana de biodiversidad]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. Mex. Biodiv.]]></abbrev-journal-title>
<issn>1870-3453</issn>
<publisher>
<publisher-name><![CDATA[Instituto de Biología]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S1870-34532018000200563</article-id>
<article-id pub-id-type="doi">10.22201/ib.20078706e.2018.2.2294</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[Genetic connectivity of the endangered brown sea cucumber Isostichopus fuscus in the northern Gulf of California revealed by novel microsatellite markers]]></article-title>
<article-title xml:lang="es"><![CDATA[Conectividad genética del pepino de mar café Isostichopus fuscus en peligro de extinción en el norte del golfo de California usando nuevos marcadores microsatelitales]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ochoa-Chávez]]></surname>
<given-names><![CDATA[José Miguel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Río-Portilla]]></surname>
<given-names><![CDATA[Miguel Ángel Del]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Calderón-Aguilera]]></surname>
<given-names><![CDATA[Luis Eduardo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rocha-Olivares]]></surname>
<given-names><![CDATA[Axayácatl]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Centro de Investigación Científica y de Educación Superior de Ensenada Molecular Ecology Laboratory Department of Biological Oceanography]]></institution>
<addr-line><![CDATA[Ensenada Baja California]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Centro de Investigación Científica y de Educación Superior de Ensenada Department of Aquaculture ]]></institution>
<addr-line><![CDATA[Ensenada Baja California]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Centro de Investigación Científica y de Educación Superior de Ensenada Coastal Zone Ecology and Fisheries Laboratory Department of Marine Ecology]]></institution>
<addr-line><![CDATA[Ensenada Baja California]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2018</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2018</year>
</pub-date>
<volume>89</volume>
<numero>2</numero>
<fpage>563</fpage>
<lpage>567</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S1870-34532018000200563&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S1870-34532018000200563&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S1870-34532018000200563&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract: Isostichopus fuscus is an economically important sea cucumber that has been highly exploited along its distribution range in the eastern Pacific. The significant population decline is responsible for its listing as endangered in the IUCN Red List. Despite its importance for management and conservation, information about its population genetic structure is unavailable, largely due to the lack of suitable genetic markers. Here we develop species-specific microsatellite markers and use them to assess the genetic connectivity between populations in the Gulf of California. Next generation sequencing (Illumina) was used to shotgun-sequence the genome of 2 sea cucumbers. From these data, we identified and characterized 19 polymorphic microsatellite loci; which were tested in organisms from Bahía de los Ángeles, on the western shore of the Gulf of California. The number of alleles ranged from 5 to 22, observed heterozygosity from 0.35 to 1, and 4 loci deviated from Hardy-Weinberg equilibrium. We determined high levels of genetic connectivity between this locality and San Felipe, in the upper gulf (Amova &#966;st = 0.002; p &gt; 0.05) with a subset of 8 markers. The newly designed microsatellites are suitable for multiplexing panels and will be useful for the future genetic assessment of this important tropical sea cucumber.]]></p></abstract>
<abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen: Isostichopus fuscus es un pepino de mar de importancia económica que ha sido altamente explotado a lo largo de su distribución en el Pacífico oriental. La reducción significativa en sus poblaciones es responsable de su inclusión en la Lista Roja de la UICN como especie amenazada de extinción. A pesar de la importancia que tiene para su manejo y conservación, no hay información sobre su estructura genética poblacional, en gran parte debido a la falta de marcadores genéticos adecuados. Para este trabajo desarrollamos marcadores microsatelitales específicos para la especie y los usamos para evaluar la conectividad genética entre poblaciones del golfo de California. Usamos secuenciación de siguiente generación (Illumina) para secuenciar por &#8220;shotgun&#8221; el genoma de 2 pepinos de mar. A partir de esos datos, identificamos y caracterizamos 19 loci microsatelitales polimórficos que fueron evaluados en organismos de Bahía de los Ángeles, en la costa occidental del golfo de California. El número de alelos varió entre 5 y 22, la heterocigosidad observada entre 0.35 y 1, y 4 loci se desviaron del equilibrio de Hardy-Weinberg. Usando 8 de estos marcadores se determinaron altos niveles de conectividad genética entre Bahía de los Ángeles y San Felipe, en el alto golfo (Amova &#966;st = 0.002; p &gt; 0.05). Los nuevos loci microsatelitales son adecuados para ser usados en páneles de multiplexación y serán útiles para la futura evaluación genética de este importante pepino del mar tropical.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[Holothurid]]></kwd>
<kwd lng="en"><![CDATA[Fisheries]]></kwd>
<kwd lng="en"><![CDATA[Conservation genetics]]></kwd>
<kwd lng="en"><![CDATA[Next generation sequencing]]></kwd>
<kwd lng="en"><![CDATA[Genome sequencing]]></kwd>
<kwd lng="es"><![CDATA[Holotúrido]]></kwd>
<kwd lng="es"><![CDATA[Pesquerías]]></kwd>
<kwd lng="es"><![CDATA[Genética de la conservación]]></kwd>
<kwd lng="es"><![CDATA[Secuenciación de siguiente generación]]></kwd>
<kwd lng="es"><![CDATA[Secuenciación genómica]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Excoffier]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
<name>
<surname><![CDATA[Laval]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
<name>
<surname><![CDATA[Schneider]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Arlequin ver. 3.0: An integrated software package for population genetics data analysis]]></article-title>
<source><![CDATA[Evolutionary Bioinformatics Online]]></source>
<year>2005</year>
<volume>1</volume>
<page-range>47-50</page-range></nlm-citation>
</ref>
<ref id="B2">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Faircloth]]></surname>
<given-names><![CDATA[B. C.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design]]></article-title>
<source><![CDATA[Molecular Ecology Resources]]></source>
<year>2008</year>
<volume>8</volume>
<page-range>92-4</page-range></nlm-citation>
</ref>
<ref id="B3">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Gardner]]></surname>
<given-names><![CDATA[M. G.]]></given-names>
</name>
<name>
<surname><![CDATA[Fitch]]></surname>
<given-names><![CDATA[A. J.]]></given-names>
</name>
<name>
<surname><![CDATA[Bertozzi]]></surname>
<given-names><![CDATA[T.]]></given-names>
</name>
<name>
<surname><![CDATA[Lowe]]></surname>
<given-names><![CDATA[A. J.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Rise of the machines - recommendations for ecologists when using next generation sequencing for microsatellite development]]></article-title>
<source><![CDATA[Molecular Ecology Resources]]></source>
<year>2011</year>
<volume>11</volume>
<page-range>1093-101</page-range></nlm-citation>
</ref>
<ref id="B4">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Holland]]></surname>
<given-names><![CDATA[M. M.]]></given-names>
</name>
<name>
<surname><![CDATA[Parson]]></surname>
<given-names><![CDATA[W.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[GeneMarker ® HID: a reliable software tool for the analysis of forensic STR data]]></article-title>
<source><![CDATA[Journal of Forensic Sciences]]></source>
<year>2011</year>
<volume>56</volume>
<page-range>29-35</page-range></nlm-citation>
</ref>
<ref id="B5">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Hong]]></surname>
<given-names><![CDATA[Y. K.]]></given-names>
</name>
<name>
<surname><![CDATA[Kim]]></surname>
<given-names><![CDATA[S. D.]]></given-names>
</name>
<name>
<surname><![CDATA[Polnefuller]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Gibor]]></surname>
<given-names><![CDATA[A. O.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[DNA extraction conditions from Porphyra perforata using LiCl]]></article-title>
<source><![CDATA[Journal of Applied Phycology]]></source>
<year>1995</year>
<volume>7</volume>
<page-range>101-7</page-range></nlm-citation>
</ref>
<ref id="B6">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Ibarra]]></surname>
<given-names><![CDATA[A. A.]]></given-names>
</name>
<name>
<surname><![CDATA[Soberón]]></surname>
<given-names><![CDATA[G. R.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Economic reasons, ecological actions and social consequences in the Mexican sea cucumber fishery]]></article-title>
<source><![CDATA[SPC Beche-de-Mer Information Bulletin]]></source>
<year>2002</year>
<volume>17</volume>
<page-range>33-6</page-range></nlm-citation>
</ref>
<ref id="B7">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Lavín]]></surname>
<given-names><![CDATA[M. F.]]></given-names>
</name>
<name>
<surname><![CDATA[Marinone]]></surname>
<given-names><![CDATA[S. G.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[An overview of the physical oceanography of the Gulf of California]]></article-title>
<person-group person-group-type="editor">
<name>
<surname><![CDATA[Velasco-Fuentes]]></surname>
<given-names><![CDATA[O.]]></given-names>
</name>
<name>
<surname><![CDATA[Sheinbaum]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
<name>
<surname><![CDATA[Ochoa]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
</person-group>
<source><![CDATA[Nonlinear processes in geophysical fluid dynamics]]></source>
<year>2003</year>
<page-range>173-204</page-range><publisher-loc><![CDATA[Dordrecht, Netherlands ]]></publisher-loc>
<publisher-name><![CDATA[Springer]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B8">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Lohr]]></surname>
<given-names><![CDATA[H. R.]]></given-names>
</name>
</person-group>
<source><![CDATA[Genetic variation among geographically isolated populations of the commercially important sea cucumber, Isostichopus fuscus, in the Eastern Pacific]]></source>
<year>2003</year>
<publisher-loc><![CDATA[CA, USA ]]></publisher-loc>
<publisher-name><![CDATA[San Francisco State University]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B9">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Maluf]]></surname>
<given-names><![CDATA[L. Y.]]></given-names>
</name>
</person-group>
<source><![CDATA[Composition and distribution of the central eastern Pacific echinoderms]]></source>
<year>1988</year>
<publisher-loc><![CDATA[Los Angeles, CA ]]></publisher-loc>
<publisher-name><![CDATA[Natural History Museum of Los Angeles County]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B10">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Park]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
</person-group>
<source><![CDATA[MStools v 3 (Excel spreadsheet toolkit for data conversion)]]></source>
<year>2001</year>
<publisher-loc><![CDATA[Dublin ]]></publisher-loc>
<publisher-name><![CDATA[Smurfit Institute of Genetics. Trinity College]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B11">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Rousset]]></surname>
<given-names><![CDATA[F.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[GENEPOP ' 007: a complete re-implementation of the GENEPOP software for Windows and Linux]]></article-title>
<source><![CDATA[Molecular Ecology Resources]]></source>
<year>2008</year>
<volume>8</volume>
<page-range>103-6</page-range></nlm-citation>
</ref>
<ref id="B12">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Schuelke]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[An economic method for the fluorescent labeling of PCR fragments]]></article-title>
<source><![CDATA[Nature Biotechnology]]></source>
<year>2000</year>
<volume>18</volume>
<page-range>233-4</page-range></nlm-citation>
</ref>
<ref id="B13">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Toral-Granda]]></surname>
<given-names><![CDATA[V.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Population status, fisheries and trade of sea cucumbers in Latin America and the Caribbean]]></article-title>
<person-group person-group-type="editor">
<name>
<surname><![CDATA[Toral-Granda]]></surname>
<given-names><![CDATA[V.]]></given-names>
</name>
<name>
<surname><![CDATA[Lovatelli]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[Vasconcellos]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<source><![CDATA[Sea cucumbers. A global review of fisheries and trade. FAO Fisheries and Aquaculture Technical Paper]]></source>
<year>2008</year>
<page-range>213-29</page-range><publisher-loc><![CDATA[Rome ]]></publisher-loc>
<publisher-name><![CDATA[FAO]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B14">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Van Oosterhout]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
<name>
<surname><![CDATA[Hutchinson]]></surname>
<given-names><![CDATA[W. F.]]></given-names>
</name>
<name>
<surname><![CDATA[Wills]]></surname>
<given-names><![CDATA[D. P. M.]]></given-names>
</name>
<name>
<surname><![CDATA[Shipley]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data]]></article-title>
<source><![CDATA[Molecular Ecology Notes]]></source>
<year>2004</year>
<volume>4</volume>
<page-range>535-8</page-range></nlm-citation>
</ref>
</ref-list>
</back>
</article>
