<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>1870-0195</journal-id>
<journal-title><![CDATA[Revista mexicana de ciencias farmacéuticas]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. mex. cienc. farm]]></abbrev-journal-title>
<issn>1870-0195</issn>
<publisher>
<publisher-name><![CDATA[Asociación Farmacéutica Mexicana A.C.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S1870-01952015000200050</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Estandarización del método de extracción de ARNm de vasos de cordón umbilical]]></article-title>
<article-title xml:lang="en"><![CDATA[Standardization of the mRNA extraction method from umbilical cord vessel]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Escalona V.]]></surname>
<given-names><![CDATA[Jesús Alejandro]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Layton T.]]></surname>
<given-names><![CDATA[Cristian Fabián]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Mendieta Z.]]></surname>
<given-names><![CDATA[Hugo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
<xref ref-type="aff" rid="A a"/>
<xref ref-type="aff" rid="A03"/>
</contrib>
</contrib-group>
<aff id="A01">
<institution><![CDATA[,Universidad Autónoma del Estado de México Facultad de Ciencias ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="A02">
<institution><![CDATA[,Universidad Autónoma del Estado de México Facultad de Medicina ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="A03">
<institution><![CDATA[,Asociación Científica Latina A.C.  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>06</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>06</month>
<year>2015</year>
</pub-date>
<volume>46</volume>
<numero>2</numero>
<fpage>50</fpage>
<lpage>53</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S1870-01952015000200050&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S1870-01952015000200050&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S1870-01952015000200050&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen: La cuantificación génica en cordón umbilical se ha utilizado para estudiar diversas patologías, no obstante está latente la problemática de homogenizar correctamente el tejido vascular. El objetivo de este estudio fue proponer una secuencia que lleva al éxito en la obtención de ARNm de los vasos de cordón umbilical. Se procesaron 20 muestras de cordón umbilical, de los cuales se disecaron los vasos para ser homogenizados en el Bullet Blender Standard BBX24 (Next Advance, Inc., Burden Lake Road, NY, USA). La extracción de ARNm se realizó en el equipo MagNa Pure LC 2.0 (Roche, Alemania), usando el kit MagNA Pure LC RNA Isolation Kit III (Tissue) (Roche, Alemania) y se cuantificó su concentración por espectrofotometría a 260 nm en un NanoPhotometer (Implen GmbH, Alemania). En el 100 % de las muestras se obtuvo ARNm.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract: Gene quantification in umbilical cord has been used to study several diseases, however the correct homogenization of the vascular tissue is a latent problem. The main objective of this study was to propose a sequence of steps leading to the successful mRNA extraction from umbilical cord vessels. 20 samples of umbilical cord, from which the vessels were dissected to be homogenized in the Bullet Blender Standard BBX24 (Next Advance, Inc., Burden Lake Road, NY, USA) were processed. mRNA extraction was performed on the MagNA Pure LC 2.0 (Roche, Germany), using the MagNA Pure LC kit RNA Isolation Kit III (Tissue) (Roche, Germany) and its concentration was quantified by spectrophotometry at 260 nm in a NanoPhotometer (Implen GmbH, Germany quantified ). mRNA was obtained in 100% of the samples.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[cordón umbilical]]></kwd>
<kwd lng="es"><![CDATA[GAPDH]]></kwd>
<kwd lng="es"><![CDATA[reacción en cadena de la polimerasa en tiempo real]]></kwd>
<kwd lng="es"><![CDATA[NF-KB]]></kwd>
<kwd lng="en"><![CDATA[umbilical cord]]></kwd>
<kwd lng="en"><![CDATA[GAPDH]]></kwd>
<kwd lng="en"><![CDATA[real time quantitative polymerase chain reaction]]></kwd>
<kwd lng="en"><![CDATA[NF-KB]]></kwd>
</kwd-group>
</article-meta>
</front><back>
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