Biconversion of (+)-nootkatone by Botryodiplodia theobromae using a membrane aerated biofilm reactor
Bioconversión de (+)-nootkaton por Botryodiplodia theobromae utilizando un reactor de biopelícula de membrana aireada
D.M. Palmerín-Carreño1, O.M. Rutiaga-Quiñones2, J.R. Verde-Calvo1, S. Huerta-Ochoa1*
1 Departamento de Biotecnología, Universidad Autónoma Metropolitana. P.A. 55-535, 09340 Iztapalapa, México D.F., México. * Corresponding author. E-mail: sho@xanum.uam.mx.
]]> 2 Departamento de Química-Bioquímica, Instituto Tecnológico de Durango, Durango.
Received July 17, 2014.
Accepted October 19, 2014.
Abstract
The aim of this work was to evaluate the bioconversion of (+)-valencene to (+)-nootkatone by B. theobromae using a membrane aerated biofilm reactor (MABR) in a two liquid phase system with orange essential oil as the organic phase. In the aqueous phase system, a (+)-nootkatone production rate up to 3.98 mg L-1 h-1 was achieved, obtaining a final product concentration of 398.08 mg L-1 with a bioconversion of 62 %. A two liquid phase system, using orange essential oil as the dispersed phase, was also studied and a final (+)-nootkatone concentration of 310.37 mg L-1 was achieved in the organic phase, with a bioconversion of 330.5 % and a production rate of 2.46 mg L-1 day-1. The lower performance obtained using the two phase system was probably due to mass transfer limitations. The present work is the first report on an MABR for the bioconversion of (+)-valencene to (+)-nootkatone. Further studies on bioconversion products and optimization of biofilm reactor operations are needed to enhance bioconversion.
Keywords: bioconversion, (+)-nootkatone, Botryodiplodia theobromae, orange essential oil, membrane aerated biofilm reactor.
Resumen
]]> El objetivo de este trabajo fue evaluar la bioconversión de (+)-valenceno a (+)-nootkaton por B. theobromae usando un reactor de biopelícula de membrana aireada (MABR) en un sistema de dos fases líquidas con aceite esencial de naranja como fase orgánica. En el sistema de fase acuosa, se logró una tasa de producción de (+)-nootkaton de hasta 3.98 mg L-1 h-1, obteniendo una concentración de producto final de 398.08 mg L 1 con una bioconversión de 62 %. También se estudio un sistema de dos fases líquidas, utilizando aceite esencial de naranja como fase dispersa, y se alcanzó una concentración final de (+)-nootkaton en la fase orgánica de 310.37 mg L-1, con una bioconversión de 30.5 % y una tasa de producción de 2.46 mg L-1 día-1. El menor rendimiento obtenido mediante el sistema de dos fases fue probablemente debido a las limitaciones de transferencia de masa. El presente trabajo es el primer reporte utilizando un MABR para la bioconversión de (+)-valenceno a (+)-nootkaton. Se necesitan estudios adicionales sobre los productos de bioconversión y la optimización de las condiciones de operación del reactor de biopelícula para mejorar la bioconversión.Palabras clave: bioconversión, (+)-nootkaton, Botryodiplodia theobromae, aceite esencial de naranja, reactor de biopelícula de membrana aireada.
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Acknowledgments
The authors are grateful for financial support from the Mexican Council of Science and Technology (CONACYT) for PhD Grant 226912.
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