Diseño de un jugo mínimamente procesado adicionado con micro y nanocápsulas de ácido fólico y su uso como vehículo para la ingesta en ratas Wistar
Design of a minimally processed juice added with micro and nanocapsules of folic acid and its use as vehicle for ingestion in wistar rats
V. Santacruz-Vázquez1*, C. Santacruz-Vázquez1 y J.O. Laguna-Cortés2
1 Benemérita Universidad Autónoma de Puebla, Facultad de Ingeniería Química, Ciudad Universitaria, 72570, Puebla, Pue., México. *Autora para la correspondencia. E-mail: versanva@gmail.com
2 Instituto Tecnológico de Puebla, 72220, Puebla, Pue., México.
]]>Recibido 7 de Noviembre de 2012
Aceptado 22 de Marzo de 2013
Resumen
Se diseñó un jugo de piña mínimamente procesado (JPMP) adicionado con micro y nanocápsulas de ácido fólico (AF) y se usó como vehículo para la ingesta de AF en un modelo experimental de ratas Wistar. Se formaron grupos con 4 diferentes dietas de hidratación. Los animales fueron sometidos durante 30 días a las dietas establecidas y a extracciones de sangre, para determinar valores de eritrocitos, hematocritos y concentración de hemoglobina. El consumo de la bebida fortificada por las ratas madre permitió valores medios significativamente mayores de hemoglobina y hematocritos en el plasma al cabo de los 30 días de tratamiento, además de un aumento mayor de la talla y peso de las crías. El modelo de von Bertalanffy describió adecuadamente los datos de peso y longitud de las ratas.
Palabras clave: nanocápsulas, ácido fólico, jugo de piña mínimamente procesado, ratas.
Abstract
A minimally processed pineapple juice added with micro- and nanocapsules of folic acid (FA) were designed and it was used as vehicle for ingestion of FA in an experimental model using Wistar rats. The rats were allocated into four groups and fed four different hydration diets for 30 days. Blood samples were collected after feeding to determine the erythrocyte, hematocrit count and hemoglobin concentration. Consumption of micro- and nanocapsules of folic-acid-fortifieded juice for 350 days resulted in significant increases in plasma hemoglobin concentrations and hematocrit, as well as increased size and weight gain of the offspring. Modd of von Bertalanffy allowed to describe models body weight and length rats data.
]]> Keywords: fortifiel food, nanoencapsulation, folic acid, minimally processed pineapple juice, rats.
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Agradecimientos
Se agradece el apoyo económico para la realización de este artículo al Consejo Nacional de Ciencia y Tecnología proyecto 2009-132983.
Se agradece el apoyo al Centro de Vinculación Universitario (BUAP) por las facilidades para el uso del microscopio electrónico de barrido, en especial al Dr. Efrain Rubio Rosas.
Referencias
A.O.A.C. (1995). Official Methods of Analysis. 16th edition Arlington Virginia: Association of Official Analytical Chemists International. E.U.A. [ Links ]
Akhtar, M.J., Khan, M.A. y Ahmad, I. (2003). Identification of photoproducts of folic acid and its degradation pathways in aqueous solution. Journal of Pharmaceutical and Biomedical Analysis 31, 579-588. [ Links ]
Barbosa, M.L.M., Borsarelli, C.D. y Mercadante, A.Z. (2005). Light stability of spray-dried bixin encapsulated with different edible polysaccharide preparations. Food Research International 38, 989-994. [ Links ]
Breithaupt, D.E. (2001). Determination of folic acid by ion-pair RP-HPLC in vitamin-fortified fruit juices after solid-phase extraction. Food Chemistry 74, 521-525. [ Links ]
Brunner, C.T., Türker Baran, E., Duarte Pinho, E., Reis, R.L. y Meleiro Neves, M. (2011). Performance of biodegradable microcapsules of poly(butylene succinate), poly(butylene succinate-co-adipate) and poly(butylene terephthalate-co-adipate) as drug encapsulation systems. Colloids and Surfaces B: Biointerfaces 84, 498-507. [ Links ]
Calero, J., Sánchez, Y.F:, Tórrez, R., Hernann, E., López, K. (2008). Elaboración y caracterización de microcápsulas gastrorresistentes de diclofenac obtenidas por gelificación iónica. Universitas 1, 27-30. [ Links ]
Capita, R. y Calleja, C.A. (2006). Evaluation of vitamin and mineral intakes and impact of snack foods on Spanish adults. Nutrition Research 26, 255-265. [ Links ]
Capó, J.T., Chanfrau, J.R., Gómez, M.J., Pardo, R.Z., y Agüero, F.S. (2004). Actividad antianémica de la Cassia grandis L. Revista Cubana Farmaceútica 38, 3. Disponible en: http://bvs.sld.cu/revistas/far/vol38_3_04/far09304.htm. Accesado: 17 septiembre 2012. [ Links ]
Carrillo-Navas, H., González-Rodea, D.A., Cruz-Olivares, J., Barrera-Pichardo, J.F., Román-Guerrero, A. y Pérez-Alonso, C.P. (2011). Storage stability and physicochemical properties of passion fruit juice microcapsules by spray-drying. Revista Mexicana de Ingeniería Química 10, 421-430. [ Links ]
Champagne, C. y Fustier, P. (2007). Microencapsulation for the improved delivery of bioactive compounds into foods. Current Opinion in Biotechnology 18, 184-190. [ Links ]
Dong, Z., Ma, Y., Havat, K., Jia, Ch., Xia, S. y Zhang, S. (2011). Morphology and release profile of microcapsules encapsulating peppermint oil by complex coacervation. Journal of Food Engineering 104, 455-460. [ Links ]
Drack, D. y Wolkenhauer, O. (2011). System approaches of Weiss and Bertalanffy and their relevance for systems biology today. Seminar in Cancer Biology 21, 150-155. [ Links ]
Fassiatou, T., Van Allen, M.I., Uh, S., Brian, R., Sibbald, B., Evans, J.A., Van den Hof, M.C., Zimmer, P., Crowley, M., Fernandez, B., Lee, N. y Niyonsenga, T. (2007). Reduction in neural-tube defects after folic acid fortification in Canada. The New England Journal of Medicine 357, 135-142. [ Links ]
Fuchs, M., Turchiuli, C., Bohin, M., Cuvelier, M., Ordonnaud, C., Peyrat M. y Dumoulin E. (2006). Encapsulation of oil in powder using spray drying and fluidized bed agglomeration. Journal of Food Engineering 75, 27-35. [ Links ]
Gharsallaoui, A. (2007). Applications of spray-drying in microencapsulation of food ingredients: An overview. Food Research International 40, 1107-1121. [ Links ]
Gliszczyńska-Świglo, A. (2007). Folates as antioxidants. Food Chemistry 101, 1480-1483. [ Links ]
Green, T.J., Skeaff, C., Whiting, S.J. y Gibson, R.S. (2003). Effect of folic acid supplementation on plasma zinc concentrations of young women. Nutrition 19, 522-523. [ Links ]
Gregory III, J.F., Quinlivan, E.P. y Davis, S.R. (2005). Integrating the issues of folate bioavailability, intake and metabolism in the era of fortification. Trends in Food Science & Technology 16, 229-240. [ Links ]
Herrera Batista, A., Taylín Zumeta Dubé, M. y González Bravo, M. (2012). Effects of folic acid on some morphometric variables of the thymus of adolescent rats with fetal alcohol syndrome. Revista Cubana de Investigaciones Biomédicas. Disponible en: http://bvs.sld.cu/revistas/ibi/vol31_1_12/ibi07112.htm. Accesado: 17 septiembre 2012. [ Links ]
Herrmann, W. y Obeid, R. (2011). The Mandatory Fortification of Staple Foods With Folic Acid. Deutsches Ärzteblatt International 108, 249-254. [ Links ]
Jayapriyaa, R. Shanmugham, J.R., Zavras, A.I., Rosner, B.A. y Giovannucci, E.L. (2010). Alcohol-folate interactions in the risk of oral cancer in women: A prospective cohort study. Cancer Epidemiology, Biomarkers & Prevention 19, 2516-2524. [ Links ]
Kam, K., Murray, J.M:, Arcot, J. y Ward, R. (2012). Fortification of parboiled rice with folic acid: Consumer acceptance and sensory evaluation. Food Research International 49, 354-363. [ Links ]
Kanakdande, D., Bhosale, R. y Singhal, R.S. (2007). Stability of cumin oleoresin microencapsulated in different combination of gum arabic, maltodextrina and modified starch. Carbohydrate Polymers 67, 536-541. [ Links ]
Lee, J.Y., Park, H.J., Lee, C.Y., Choi, W.Y. (2003). Extending shelf-life of minimally processed apples with edible coatings and antibrowning agents. LWT-Food Science and Technology 36, 323-329. [ Links ]
Li, X., Fang, Y., Al-Assaf, S., Phillips, G.O., Nishinari, K. y Zhang, H. (2009). Rheological study of gum arabic solutions: Interpretation based on molecular self-association. Food Hydrocolloids 23, 2394-2402. [ Links ]
Linabery, A.M., Johnson, K.J. y Ross, J.A. (2012). Childhood Cancer Incidence Trends in Association With US Folic Acid Fortification (1986-2008). Pediatrics 129, 1125-1133. [ Links ]
Liu, J., Hesson, L.B., Meagher, A.P., Bourke, M.J., Hawkins, N.J., Rand, K.N., Molloy, P.L., Pimanda, J.E. y Ward, R.L. (2012). Relative distribution of folate species is associated with global DNA methylation in human colorectal mucosa. Cancer Prevention Research 5, 921-929. [ Links ]
Macrae, R., Robinson, R.K. y Sadler, M.J. (1993). Encyclopedia of Food Science, Food Technology and Nutrition. Academic Press. New York. [ Links ]
Madene, A., Jacquot, M., Scher, J. y Desobry, S. (2006). Flavour encapsulation and controlled release a review. International Journal Food Science & Technology 41,1-21. [ Links ]
Madziva, H., Kailasaphaty, K. y Phillips, M. (2006). Evaluation of alginatepectin capsules in cheddar cheese as s food carrier for the delivery of folic acid. Journal Food Science and Technology 39, 146-151. [ Links ]
Mercadante, A.Z., Barbosa, M.L.M. y Borsarelli, C.D. (2005). Light stability of spray-dried bixin encapsulated with different edible polysaccharide preparations. Food Research International 38, 989-994. [ Links ]
Merck Index. (1989). Encyclopedia of Chemicals, Drugs and Biologicals. 9a ed.: Merck Company, Estados Unidos. [ Links ]
Moreno L. (2003). Dependencia en la temperatura de cambio en atributos sensoriales, inactivación enzimática y microbiana y degradación de ácido ascórbico durante tratamientos de pasterización en purés y néctares de mango-piña. Tesis de maestría de Ciencias de Alimentos. Universidad de las Américas-Puebla, México. [ Links ]
Mosqueda-Melgar, J., Raybaudi-Massilia, R.M. y Martín-Belloso, O. (2012). Microbiological shelf life and sensory evaluation of fruits juices treated by high-intensity pulsed electric fields and antimicrobials. Food and Bioproducts Processing 90, 205-214. [ Links ]
Moura, M.R., Aouada, F.A., Avena-Bustillos, R.J., McHugh, T.H., Krochta, J.M., Mattoso, L.H.C. (2009). Improved barrier and mechanical properties of novel hydroxypropyl methylcellulose edible films with chitosan/tripolyphosphate nanoparticles. Journal of Food Engineering 92, 448-453. [ Links ]
Noguera, R.R., Pereira, R.L. y Solarte, C.E. (2008). Comparación de modelos no lineales para describir curvas de crecimiento en cuyes (cavia porcellus) desde el nacimiento hasta la edad de sacrificio. Livestock Research for Rural Development 20, 79. [ Links ]
Norma Oficial Mexicana -062-ZOO-1999. (1999). Especificaciones técnicas para la producción, cuidado y uso de los animales de laboratorio. Disponible en: http://www.fmvz.unam.mx/fmvz/principal/archivos/062ZOO.PDF. Accesado:14 de septiembre 2012. [ Links ]
Norma Oficial Mexicana -093-SSA1-1994. (1994). http://www.bidihmujer.salud.gob.mx/documentos/leyes/NOM-093-SSA1-1994%20preparacion%20alimentos%20establec%20fijos.pdf. Accesado: 17 septiembre 2012. [ Links ]
Paschoal-Prado, R., Fornazari dos Santos, B., Lombardi de Souza Pinto, C., Carvalho de Assis, K.R., Fávero Salvadori, D.M. y Plácido Ladeira, M.S. (2010). Influence of diet on oxidative DNA damage, uracil misincorporation and DNA repair capability. Mutagenesis 25, 483-487. [ Links ]
Pérez-Alonso, C., Fabela-Morón, M.F., Guadarrama-Lezama, A.Y., Barrera-Pichardo, J.F., Alamilla- Beltrán, L. y Rodríguez-Hueso, M.E. (2009). Interrelationship between the structural features and rehydration properties of spray dried manzano chilli sauce microcapsules. Revista Mexicana de Ingeniería Química 8, 187-196. [ Links ]
Phillips, A., Williams, P.A. (2003). Handbook of hydrocolloids. Editorial Woodhead, Estados Unidos. [ Links ]
Pulido, A. y Beristain, C.I. (2010). Encapsulación de ácido ascórbico mediante secado por aspersión utilizando quitosano como material de pared. Revista Mexicana de Ingeniería Química 9, 189-195. [ Links ]
Quintanilla-Carvajal, M.X., Meraz-Torres, L.S., Alamilla-Beltrán, L., Chanona-Pérez, J.J., Jaramillo, F.M.E., Hernández-Sánchez, H., Jiménez-Aparicio, A.R. y Gutiérrez-López, G.F. (2010). Eficiencia de encapsulación de micro y nanoemulsiones de alfa tocoferol y evaluación de la morfología de las microcápsulas. Presentación ALI33420091229. 24-26 Marzo. Acapulco, Guerrero. XVIIII Congreso Nacional de Ingeniería Bioquímica, 2010. [ Links ]
Quintanilla-Carvajal, M.X., Meraz-Torres, L.S., Alamilla-Beltrán, L., Chanona-Pérez, J.J., Terres-Rojas, E., Hernández-Sánchez, H., Jiménez-Aparicio, A.R. y Gutiérrez-López, G.F. (2011). Caracterización morfométrica de microcápsulas secadas por aspersión antes y después de la extracción de α-tocoferol. Revista Mexicana de Ingeniería Química 10, 273-279. [ Links ]
Reza Avadi, M., Sadeghi, A.M.M., Mohammdpour, N., Abedin, S., Atyabi, F., Dinarvand, R. y Rafiee-Tehrani, M. (2010). Preparation and characterization of insulin nanoparticles using chitosan and Arabic gum with ionic gelation method. Nanomedicine: Nanotechnology, Biology and Medicine 6, 58-63. [ Links ]
Román-Román, P., Romero, D., Torres-Ruiz, F. (2010). A diffusion process to model generalized von Bertalanffy growth patterns: Fitting to real data. Journal of Theoretical Biology 263, 59-69. [ Links ]
Sáez, P.B. y Rittman, B.E. (1992). Model-parameter estimation using least squares. Water Research 26, 789-796. [ Links ]
Santacruz-Vázquez, V., Santacruz-Vázquez, C., Welti-Chanes, J., Farrera-Rebollo, R.R., Alamilla-Beltrán, L., Chanona-Pérez, J. y Gutiérrez-López, G.F. (2008). Effects of airdrying on the shrinkage, surface temperatures and structural features of apples slabs by means of fractal analysis. Revista Mexicana de Ingeniería Química 7, 55-63. [ Links ]
Teixeira. M., Andrade, L.R., Farina, M. y Rocha-Leão, M.H.M. (2004). Characterization of short chain fatty acid microcapsules produced by spray drying. Materials Science and Engineering C 24, 653-658. [ Links ]
Tzu-Yuan, Ch., Kang-Ju, Ch., Chin-Feng, T., Hsiao-Min, Ch., Hua-Chang, F., Yao-Min, H., Ming-Jei, W., Huey-Ming, T. y Kuo-Cheng, Lu. (2007). Effects of folic acid and vitamin B complex on serum C -reactiveprotein and albumin levels in stable hemodialysis patients. Current Medical Research & Opinion 23, 1879-1886. [ Links ]
Vora, A., Riga, R., Dollimore, D. y Kenneth, S.A.R. (2002). Thermal stability of folic acid. Thermochimica Acta 392-393, 209-220. [ Links ]
Wang, X., Yucel, T., Lu, Q., Hu, X. y Kaplan, D.L. (2010). Silk nanospheres and microspheres from silk/pva blend films for drug delivery. Biomaterials 31, 1025-1035. [ Links ]
Weinbreck, F., Minor, M. y De Kruif, C.G. (2004). Microencapsulation of oils using whey protein/gum Arabic coacervates. Journal of Microencapsulation 21. 667-679. [ Links ]
Yadav, M.P., Igartuburu, J.M., Yan, Y. y Nothnagel, N.A. (2007). Chemical investigation of the structural basis of the emulsifying activity of gum arabic. Food Hydrocolloid 21, 297-298. [ Links ]
Young, D.K. y Morr, Ch.V. (2006). Microencapsulation properties of gum arabic and several food proteins: Liquid orange oil emulsion particles. Journal of Agricultural Food Chemistry 44, 1314-1320. [ Links ]
Younis, I.R., Stamatakis, M.K., Callery, P.S. y Meyer-Stout, P.J. (2009). Influence of pH on the dissolution of folic acid supplements. International Journal of Pharmaceutics 367, 97-102. [ Links ]
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