<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>1405-888X</journal-id>
<journal-title><![CDATA[TIP. Revista especializada en ciencias químico-biológicas]]></journal-title>
<abbrev-journal-title><![CDATA[TIP]]></abbrev-journal-title>
<issn>1405-888X</issn>
<publisher>
<publisher-name><![CDATA[Universidad Nacional Autónoma de México, Facultad de Estudios Superiores Zaragoza]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S1405-888X2018000321105</article-id>
<article-id pub-id-type="doi">10.22201/fesz.23958723e.2018.0.148</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[An interpromoter region enhances transcription of argK gene, encoding the phaseolotoxin-resistant ornithine carbamoyltransferase in Pseudomonas syringae pv. phaseolicola]]></article-title>
<article-title xml:lang="es"><![CDATA[Una región interpromotora incrementa la transcripción del gen argK , que codifica para la ornitina carbamoiltransferasa resistente a la faseolotoxina en Pseudomonas syringae pv. phaseolicola]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Hernández-Flores]]></surname>
<given-names><![CDATA[José Luis]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Álvarez-Morales]]></surname>
<given-names><![CDATA[Ariel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Torre-Zavala]]></surname>
<given-names><![CDATA[Susana De la]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Hernández-Soriano]]></surname>
<given-names><![CDATA[Laura]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Chacón-López]]></surname>
<given-names><![CDATA[Alejandra]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Montalvo-González]]></surname>
<given-names><![CDATA[Efigenia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Aguilera]]></surname>
<given-names><![CDATA[Selene]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Instituto Politécnico Nacional Centro de Investigación y de Estudios Avanzados (CINVESTAV) Departamento de Ingeniería Genética]]></institution>
<addr-line><![CDATA[ Guanajuato]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad Autónoma de Nuevo León Facultad de Ciencias Biológicas Instituto de Biotecnología]]></institution>
<addr-line><![CDATA[ Nuevo León]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Instituto Tecnológico de Tepic Laboratorio Integral de Investigación en Alimentos ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af4">
<institution><![CDATA[,Instituto Tecnológico de Tepic Laboratorio Integral de Investigación en Alimentos ]]></institution>
<addr-line><![CDATA[Tepic Nayarit]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2018</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2018</year>
</pub-date>
<volume>21</volume>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S1405-888X2018000321105&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S1405-888X2018000321105&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S1405-888X2018000321105&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract Pseudomonas syringae pv. phaseolicola, is the causal agent of halo blight on bean, a disease characterized by water soaked lesions surrounded by a chlorotic halo, which is produced by the action of phaseolotoxin, a nonhost-specific toxin optimally produced between 18°C to 20°C. To protect itself against phaseolotoxin, P. syringae pv. phaseolicola possesses a phaseolotoxin-resistant OCTase (ROCT) encoded by argK, which is expressed under conditions leading to the synthesis of phaseolotoxin. The promoter of argK is divergent to that of phtABC genes and both share an interpromoter region. The objective of the present study was to evaluate the role of the interpromoter region on argK transcription in P. syringae pv. phaseolicola. To this, deletions of the interpromoter region were made and argK expression was evaluated in an heterologous system. Also, the effect of multiple copies of the interpromoter region on phaseolotoxin production was determined. The obtained results showed that this region is necessary to enhance the transcription of argK. Additionally, this region in a multiple copies, interfered with the thermoregulation of phaseolotoxin at 28°C in strain P. syringae pv. phaseolicola NPS3121.]]></p></abstract>
<abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen Pseudomonas syringae pv. phaseolicola, es el agente causal del tizón del halo del frijol, una enfermedad que se caracteriza por presentar lesiones acuosas rodeadas por un halo clorótico, el cual es producido por la acción de la faseolotoxina, una toxina no específica del hospedero, producida de manera óptima entre 18°C a 20°C. Para protegerse de su propia toxina, P. syringae pv. phaseolicola posee una ornitina carbamoyl-transferasa (OCTasa) resistente a la faseolotoxina (ROCT) codificada por argK, que es expresada bajo las condiciones de síntesis de la faseolotoxina. El promotor de argK es divergente al promotor de phtABC y ambos comparten una región interpromotora. El objetivo del presente estudio fue evaluar la función de la región interpromotora sobre la transcripción de argK en P. syringae pv. phaseolicola. Para esto, se llevaron a cabo deleciones de la región interpromotora y se evaluó la transcripción de argK en un sistema heterólogo. También se determinó el efecto de múltiples copias de la región interpromotora sobre la producción de la faseolotoxina. Los resultados obtenidos mostraron que esta región es necesaria para incrementar la transcripción de argK. Adicionalmente, cuando se ponen múltiples copias de esta región, se interfiere con la termorregulación de la faseolotoxina a 28°C en la cepa de P. syringae pv. phaseolicola NPS3121.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[phaseolotoxin]]></kwd>
<kwd lng="en"><![CDATA[argK gene]]></kwd>
<kwd lng="en"><![CDATA[promoter]]></kwd>
<kwd lng="en"><![CDATA[thermoregulation]]></kwd>
<kwd lng="en"><![CDATA[interpromoter region]]></kwd>
<kwd lng="es"><![CDATA[faseolotoxina]]></kwd>
<kwd lng="es"><![CDATA[gen argK]]></kwd>
<kwd lng="es"><![CDATA[promotor]]></kwd>
<kwd lng="es"><![CDATA[terrmorregulación]]></kwd>
<kwd lng="es"><![CDATA[región interpromotora]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Aguilera]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[De la Torre-Zavala]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Hernández-Flores]]></surname>
<given-names><![CDATA[J. L.]]></given-names>
</name>
<name>
<surname><![CDATA[Murillo]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
<name>
<surname><![CDATA[Bravo]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
<name>
<surname><![CDATA[Álvarez-Morales]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Expression of the gene for resistance to phaseolotoxin (argK) depends on the activity of genes phtABC in Pseudomonas syringae pv. phaseolicola]]></article-title>
<source><![CDATA[PLoS One]]></source>
<year>2012</year>
<volume>7</volume>
<numero>10</numero>
<issue>10</issue>
</nlm-citation>
</ref>
<ref id="B2">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Aguilera]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[López-López]]></surname>
<given-names><![CDATA[K.]]></given-names>
</name>
<name>
<surname><![CDATA[Nieto]]></surname>
<given-names><![CDATA[Y.]]></given-names>
</name>
<name>
<surname><![CDATA[Garciduenas-Piña]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
<name>
<surname><![CDATA[Hernández-Guzmán]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
<name>
<surname><![CDATA[Hernández-Flores]]></surname>
<given-names><![CDATA[J. L.]]></given-names>
</name>
<name>
<surname><![CDATA[Murillo]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
<name>
<surname><![CDATA[Álvarez-Morales]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Functional characterization of the gene cluster from Pseudomonas syringae pv. phaseolicola NPS3121 involved in synthesis of phaseolotoxin]]></article-title>
<source><![CDATA[Journal of Bacteriology]]></source>
<year>2007</year>
<volume>189</volume>
<page-range>2834-43</page-range></nlm-citation>
</ref>
<ref id="B3">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Bachmann]]></surname>
<given-names><![CDATA[A.S.]]></given-names>
</name>
<name>
<surname><![CDATA[Matile]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
<name>
<surname><![CDATA[Slusarenko]]></surname>
<given-names><![CDATA[A.J.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Inhibition of ornithine decarboxylase activity by phaseolotoxin: implications for symptom production in halo blight of French bean]]></article-title>
<source><![CDATA[Physiol Mol Plant Pathol.]]></source>
<year>1998</year>
<volume>53</volume>
<numero>5-6</numero>
<issue>5-6</issue>
<page-range>287-99</page-range></nlm-citation>
</ref>
<ref id="B4">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Chen]]></surname>
<given-names><![CDATA[W. P.]]></given-names>
</name>
<name>
<surname><![CDATA[Kuo]]></surname>
<given-names><![CDATA[T. T.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[A simple and rapid method for the preparation of gram-negative bacterial genomic DNA]]></article-title>
<source><![CDATA[Nucleic Acids Research]]></source>
<year>1993</year>
<volume>21</volume>
<numero>9</numero>
<issue>9</issue>
<page-range>2260</page-range></nlm-citation>
</ref>
<ref id="B5">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Dombroski]]></surname>
<given-names><![CDATA[A. J.]]></given-names>
</name>
<name>
<surname><![CDATA[Walter]]></surname>
<given-names><![CDATA[W. A.]]></given-names>
</name>
<name>
<surname><![CDATA[Record]]></surname>
<given-names><![CDATA[M. T. Jr.]]></given-names>
</name>
<name>
<surname><![CDATA[Siegele]]></surname>
<given-names><![CDATA[D. A.]]></given-names>
</name>
<name>
<surname><![CDATA[Gross]]></surname>
<given-names><![CDATA[C. A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Polypeptides containing highly conserved regions of transcription initiation factor sigma 70 exhibit specificity of binding to promoter DNA]]></article-title>
<source><![CDATA[Cell]]></source>
<year>1992</year>
<volume>70</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>501-12</page-range></nlm-citation>
</ref>
<ref id="B6">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Ferguson]]></surname>
<given-names><![CDATA[A.R.]]></given-names>
</name>
<name>
<surname><![CDATA[Johnston]]></surname>
<given-names><![CDATA[J.S.]]></given-names>
</name>
<name>
<surname><![CDATA[Mitchell]]></surname>
<given-names><![CDATA[R.E.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Resistance of Pseudomonas syringae pv. phaseolicola to its own toxin, phaseolotoxin]]></article-title>
<source><![CDATA[Fems Microbiological Letters]]></source>
<year>1980</year>
<volume>7</volume>
<page-range>123-5</page-range></nlm-citation>
</ref>
<ref id="B7">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Genka]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
<name>
<surname><![CDATA[Baba]]></surname>
<given-names><![CDATA[T.]]></given-names>
</name>
<name>
<surname><![CDATA[Tsuda]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Kanaya]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Mori]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
<name>
<surname><![CDATA[Yoshida]]></surname>
<given-names><![CDATA[T.]]></given-names>
</name>
<name>
<surname><![CDATA[Noguchi]]></surname>
<given-names><![CDATA[M. T.]]></given-names>
</name>
<name>
<surname><![CDATA[Tsuchiya]]></surname>
<given-names><![CDATA[K.]]></given-names>
</name>
<name>
<surname><![CDATA[Sawada]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Comparative analysis of argK-tox clusters and their flanking regions in phaseolotoxin-producing Pseudomonas syringae pathovars]]></article-title>
<source><![CDATA[Journal of Molecular Evolution]]></source>
<year>2006</year>
<volume>63</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>401-14</page-range></nlm-citation>
</ref>
<ref id="B8">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Goss]]></surname>
<given-names><![CDATA[R.W.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[The relation of temperature to common and halo blight of beans]]></article-title>
<source><![CDATA[Phytopathology]]></source>
<year>1940</year>
<volume>30</volume>
<page-range>258-64</page-range></nlm-citation>
</ref>
<ref id="B9">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Hatziloukas]]></surname>
<given-names><![CDATA[E.]]></given-names>
</name>
<name>
<surname><![CDATA[Panopoulos]]></surname>
<given-names><![CDATA[N. J.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Origin, structure, and regulation of argK, encoding the phaseolotoxin resistant ornithine carbamoyltransferase in Pseudomonas syringae pv. phaseolicola, and functional expression of argK in transgenic tobacco]]></article-title>
<source><![CDATA[Journal of Bacteriolog.]]></source>
<year>1992</year>
<volume>174</volume>
<page-range>5895-909</page-range></nlm-citation>
</ref>
<ref id="B10">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Hernández-Guzmán]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
<name>
<surname><![CDATA[Álvarez-Morales]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Isolation and characterization of the gene coding for the amidinotransferase involved in the biosynthesis of phaseolotoxin in Pseudomonas syringae pv. phaseolicola]]></article-title>
<source><![CDATA[Molecular Plant Microbe Interaction]]></source>
<year>2001</year>
<volume>14</volume>
<page-range>545-54</page-range></nlm-citation>
</ref>
<ref id="B11">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Jahn]]></surname>
<given-names><![CDATA[O.]]></given-names>
</name>
<name>
<surname><![CDATA[Sauerstein]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
<name>
<surname><![CDATA[Reuter]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Detection of two ornithine carbamoyltransferases in a phaseolotoxin-producing strain Pseudomonas syringae pv. phaseolicola]]></article-title>
<source><![CDATA[Journal of Basic Microbiology]]></source>
<year>1985</year>
<volume>25</volume>
<page-range>543-6</page-range></nlm-citation>
</ref>
<ref id="B12">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Jahn]]></surname>
<given-names><![CDATA[O.]]></given-names>
</name>
<name>
<surname><![CDATA[Sauerstein]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
<name>
<surname><![CDATA[Reuter]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Characterization of two ornithine carbamoyltransferases from Pseudomonas syringae pv. phaseolicola, the producer of phaseolotoxin]]></article-title>
<source><![CDATA[Archives of Microbiology]]></source>
<year>1987</year>
<volume>147</volume>
<page-range>174-8</page-range></nlm-citation>
</ref>
<ref id="B13">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[King]]></surname>
<given-names><![CDATA[E. O.]]></given-names>
</name>
<name>
<surname><![CDATA[Ward]]></surname>
<given-names><![CDATA[M. K.]]></given-names>
</name>
<name>
<surname><![CDATA[Raney]]></surname>
<given-names><![CDATA[D. E.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Two simple media for the demonstration of pyocyanin and fluorescin]]></article-title>
<source><![CDATA[J. Lab. Clin. Med]]></source>
<year>1954</year>
<volume>44</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>301-7</page-range></nlm-citation>
</ref>
<ref id="B14">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[López-López]]></surname>
<given-names><![CDATA[K.]]></given-names>
</name>
<name>
<surname><![CDATA[Hernández-Flores]]></surname>
<given-names><![CDATA[J. L.]]></given-names>
</name>
<name>
<surname><![CDATA[Cruz-Aguilar]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Álvarez-Morales]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[In Pseudomonas syringae pv. phaseolicola, expression of the argK gene, encoding the phaseolotoxin-resistant ornithine carbamoyltransferase, is regulated indirectly by temperature and directly by a precursor resembling carbamoylphosphate]]></article-title>
<source><![CDATA[Journal of Bacteriology]]></source>
<year>2004</year>
<volume>186</volume>
<page-range>146-53</page-range></nlm-citation>
</ref>
<ref id="B15">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Mitchell]]></surname>
<given-names><![CDATA[R.E.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Isolation and structure of a chlorosis-inducing toxin of Pseudomonas phaseolicola]]></article-title>
<source><![CDATA[Phytochemistr]]></source>
<year>1976</year>
<volume>15</volume>
<page-range>1941-7</page-range></nlm-citation>
</ref>
<ref id="B16">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Mitchell]]></surname>
<given-names><![CDATA[R.E.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Halo blight of beans: toxin production by several Pseudomonas phaseolicola isolates]]></article-title>
<source><![CDATA[Physiology of Plant Pathology]]></source>
<year>1978</year>
<volume>13</volume>
<page-range>37-49</page-range></nlm-citation>
</ref>
<ref id="B17">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Mosqueda]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
<name>
<surname><![CDATA[Van den Broeck]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
<name>
<surname><![CDATA[Saucedo]]></surname>
<given-names><![CDATA[O.]]></given-names>
</name>
<name>
<surname><![CDATA[Bailey]]></surname>
<given-names><![CDATA[A. M.]]></given-names>
</name>
<name>
<surname><![CDATA[Álvarez-Morales]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[Herrera-Estrella]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Isolation and characterization of the gene from Pseudomonas syringae pv. phaseolicola encoding the phaseolotoxin-insensitive ornithine carbamoyltransferase]]></article-title>
<source><![CDATA[Molecular General Genetis]]></source>
<year>1990</year>
<volume>222</volume>
<page-range>461-6</page-range></nlm-citation>
</ref>
<ref id="B18">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Nuske]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
<name>
<surname><![CDATA[Fritsche]]></surname>
<given-names><![CDATA[W.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Phaseolotoxin production by Pseudomonas syringae pv. phaseolicola: the influence of temperature]]></article-title>
<source><![CDATA[Journal of Basic Microbiology]]></source>
<year>1989</year>
<volume>29</volume>
<page-range>441-7</page-range></nlm-citation>
</ref>
<ref id="B19">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Peet]]></surname>
<given-names><![CDATA[R. C.]]></given-names>
</name>
<name>
<surname><![CDATA[Lindgren]]></surname>
<given-names><![CDATA[P. B.]]></given-names>
</name>
<name>
<surname><![CDATA[Willis]]></surname>
<given-names><![CDATA[D. K.]]></given-names>
</name>
<name>
<surname><![CDATA[Panopoulos]]></surname>
<given-names><![CDATA[N. J.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Identification and cloning of genes involved in phaseolotoxin production by Pseudomonas syringae pv. "phaseolicola"]]></article-title>
<source><![CDATA[Journal of Bacteriology]]></source>
<year>1986</year>
<volume>166</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>1096-105</page-range></nlm-citation>
</ref>
<ref id="B20">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Rico]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[López]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
<name>
<surname><![CDATA[Asensio]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
<name>
<surname><![CDATA[Aizpun]]></surname>
<given-names><![CDATA[M. T.]]></given-names>
</name>
<name>
<surname><![CDATA[Asensio-S-Manzanera]]></surname>
<given-names><![CDATA[MC.]]></given-names>
</name>
<name>
<surname><![CDATA[Murillo]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Nontoxigenic strains of Pseudomonas syringae pv. phaseolicola are a main cause of halo blight of beans in Spain and escape current detection methods]]></article-title>
<source><![CDATA[Phytopathology]]></source>
<year>2003</year>
<volume>93</volume>
<numero>12</numero>
<issue>12</issue>
<page-range>1553-9</page-range></nlm-citation>
</ref>
<ref id="B21">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Ross]]></surname>
<given-names><![CDATA[W.]]></given-names>
</name>
<name>
<surname><![CDATA[Gosink]]></surname>
<given-names><![CDATA[K. K.]]></given-names>
</name>
<name>
<surname><![CDATA[Salomon]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
<name>
<surname><![CDATA[Igarashi]]></surname>
<given-names><![CDATA[K.]]></given-names>
</name>
<name>
<surname><![CDATA[Zou]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
<name>
<surname><![CDATA[Ishihama]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[Severinov]]></surname>
<given-names><![CDATA[K.]]></given-names>
</name>
<name>
<surname><![CDATA[Gourse]]></surname>
<given-names><![CDATA[R. L.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[A third recognition element in bacterial promoters: DNA binding by the alpha subunit of RNA polymerase]]></article-title>
<source><![CDATA[Science]]></source>
<year>1993</year>
<volume>262</volume>
<numero>5138</numero>
<issue>5138</issue>
<page-range>1407-13</page-range></nlm-citation>
</ref>
<ref id="B22">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Rowley]]></surname>
<given-names><![CDATA[K. B.]]></given-names>
</name>
<name>
<surname><![CDATA[Clements]]></surname>
<given-names><![CDATA[D. E.]]></given-names>
</name>
<name>
<surname><![CDATA[Mandel]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Humphreys]]></surname>
<given-names><![CDATA[T.]]></given-names>
</name>
<name>
<surname><![CDATA[Patil]]></surname>
<given-names><![CDATA[S. S.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Multiple copies of a DNA sequence from Pseudomonas syringae pathovar phaseolicola abolish thermoregulation of phaseolotoxin production]]></article-title>
<source><![CDATA[Molecular Microbiology]]></source>
<year>1993</year>
<volume>8</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>625-35</page-range></nlm-citation>
</ref>
<ref id="B23">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Rowley]]></surname>
<given-names><![CDATA[K. B.]]></given-names>
</name>
<name>
<surname><![CDATA[Xu]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
<name>
<surname><![CDATA[Patil]]></surname>
<given-names><![CDATA[S. S.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Molecular analysis of thermoregulation of phaseolotoxin-resistant ornithine carbamoyltransferase (argK) from Pseudomonas syringae pv. phaseolicola]]></article-title>
<source><![CDATA[Molecular Plant Microbe Interactions]]></source>
<year>2000</year>
<volume>13</volume>
<numero>10</numero>
<issue>10</issue>
<page-range>1071-80</page-range></nlm-citation>
</ref>
<ref id="B24">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Sambrook]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
<name>
<surname><![CDATA[Fritsch]]></surname>
<given-names><![CDATA[E. F.]]></given-names>
</name>
<name>
<surname><![CDATA[Maniatis]]></surname>
<given-names><![CDATA[T.]]></given-names>
</name>
</person-group>
<source><![CDATA[Molecular cloning: a laboratory manual]]></source>
<year>1989</year>
<edition>2nd ed</edition>
<publisher-loc><![CDATA[Cold Spring Harbor, NY ]]></publisher-loc>
<publisher-name><![CDATA[Cold Spring Harbor Laboratory]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B25">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Staskawicz]]></surname>
<given-names><![CDATA[B. J.]]></given-names>
</name>
<name>
<surname><![CDATA[Panopoulos]]></surname>
<given-names><![CDATA[N. J.]]></given-names>
</name>
<name>
<surname><![CDATA[Hoogenraad]]></surname>
<given-names><![CDATA[N. J.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Phaseolotoxin insensitive ornithine carbamoyltransferase of Pseudomonas syringae pv. phaseolicola: basis for immunity to phaseolotoxin]]></article-title>
<source><![CDATA[Journal of Bacteriology]]></source>
<year>1980</year>
<volume>142</volume>
<page-range>720-3</page-range></nlm-citation>
</ref>
<ref id="B26">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Tamura]]></surname>
<given-names><![CDATA[K.]]></given-names>
</name>
<name>
<surname><![CDATA[Imamura]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Yoneyama]]></surname>
<given-names><![CDATA[K.]]></given-names>
</name>
<name>
<surname><![CDATA[Kohno]]></surname>
<given-names><![CDATA[Y.]]></given-names>
</name>
<name>
<surname><![CDATA[Takikawa]]></surname>
<given-names><![CDATA[Y.]]></given-names>
</name>
<name>
<surname><![CDATA[Yamaguchi]]></surname>
<given-names><![CDATA[I.]]></given-names>
</name>
<name>
<surname><![CDATA[Takahashi]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Role of phaseolotoxin production by Pseudomonas syringae pv. actinidiae in the formation of halo lesions of kiwifruit canker disease]]></article-title>
<source><![CDATA[Physiology and Molecular Plant Patholog.]]></source>
<year>2002</year>
<volume>60</volume>
<page-range>207-14</page-range></nlm-citation>
</ref>
<ref id="B27">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Tourte]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
<name>
<surname><![CDATA[Manceau]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[A strain of Pseudomonas syringae which does not belong to pathovar phaseolicola produces phaseolotoxin]]></article-title>
<source><![CDATA[European Journal of Plant Pathology]]></source>
<year>1995</year>
<volume>101</volume>
<page-range>483-90</page-range></nlm-citation>
</ref>
<ref id="B28">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[West]]></surname>
<given-names><![CDATA[S. E.]]></given-names>
</name>
<name>
<surname><![CDATA[Schweizer]]></surname>
<given-names><![CDATA[H. P.]]></given-names>
</name>
<name>
<surname><![CDATA[Dall]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
<name>
<surname><![CDATA[Sample]]></surname>
<given-names><![CDATA[A. K.]]></given-names>
</name>
<name>
<surname><![CDATA[Runyen-Janecky]]></surname>
<given-names><![CDATA[L. J.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Construction of improved Escherichia-Pseudomonas shuttle vectors derived from pUC18/19 and sequence of the region required for their replication in Pseudomonas aeruginosa]]></article-title>
<source><![CDATA[Gene]]></source>
<year>1994</year>
<volume>148</volume>
<page-range>81-6</page-range></nlm-citation>
</ref>
</ref-list>
</back>
</article>
