<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>1405-888X</journal-id>
<journal-title><![CDATA[TIP. Revista especializada en ciencias químico-biológicas]]></journal-title>
<abbrev-journal-title><![CDATA[TIP]]></abbrev-journal-title>
<issn>1405-888X</issn>
<publisher>
<publisher-name><![CDATA[Universidad Nacional Autónoma de México, Facultad de Estudios Superiores Zaragoza]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S1405-888X2018000200200</article-id>
<article-id pub-id-type="doi">10.22201/fesz.23958723e.2018.2.5</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[El sistema de edición genética CRISPR/Cas y su uso como antimicrobiano específico]]></article-title>
<article-title xml:lang="en"><![CDATA[The genetic edition system CRISPR/Cas and its use as specific antimicrobial]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Chávez-Jacobo]]></surname>
<given-names><![CDATA[Víctor M.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Michoacana de San Nicolás de Hidalgo Instituto de Investigaciones Químico-Biológicas Laboratorio de Microbiología]]></institution>
<addr-line><![CDATA[Morelia Michoacán]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2018</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2018</year>
</pub-date>
<volume>21</volume>
<numero>2</numero>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S1405-888X2018000200200&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S1405-888X2018000200200&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S1405-888X2018000200200&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen El sistema CRISPR/Cas es parte de un sistema inmune adaptativo que los organismos procariotas desarrollaron para defenderse de la incorporación de material genético exógeno. Este sistema de inmunidad está mediado por una nucleasa específica que degrada al DNA invasor y posteriormente algunos fragmentos de la molécula degradada se almacenan para reconocer y eliminar secuencias similares en el futuro. Recientemente fue posible reprogramar este sistema para reconocer cualquier secuencia de DNA y realizar ediciones genéticas en una gran cantidad de organismos de manera altamente específica. El sistema CRISPR/Cas ha sido adaptado para el desarrollo de una estrategia altamente específica en el tratamiento de infecciones producidas por bacterias resistentes a antimicrobianos. En esta revisión se describe de manera general el origen biológico del sistema CRISPR/Cas, el mecanismo de edición genética desarrollado con base en él, la aplicación del sistema en el desarrollo de un método específico para tratar infecciones bacterianas y finalmente se discutirán algunas limitaciones del sistema.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract CRISPR/Cas system constitutes a prokaryotic adaptive immune system against the incorporation of exogenous genetic material. This immunity system is mediated by a specific nuclease that breaks invasive DNA and later some particles of the broken DNA are stored to recognize and eliminate similar sequences in the future. Recently, it was found that the system could be reprogrammed to recognize any DNA sequence and it was possible to introduce highly specific genetic editions in a huge number of organisms. Furthermore, CRISPR/Cas system has been adapted for the development of a highly specific strategy in the treatment of infections caused by antimicrobial-resistant bacteria. In this review it was described in a general manner the biological origin of CRISPR/Cas system, the mechanism of genetic edition based on it, the employ of the system in the development of a specific treatment method for bacterial infections, and finally some limitations of the system were discussed.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[manipulación genética]]></kwd>
<kwd lng="es"><![CDATA[nucleasa]]></kwd>
<kwd lng="es"><![CDATA[resistencia a antimicrobianos]]></kwd>
<kwd lng="es"><![CDATA[sistema CRISPR/Cas]]></kwd>
<kwd lng="es"><![CDATA[sistema inmune adaptativo]]></kwd>
<kwd lng="en"><![CDATA[genetic manipulations]]></kwd>
<kwd lng="en"><![CDATA[nuclease]]></kwd>
<kwd lng="en"><![CDATA[antimicrobial resistance]]></kwd>
<kwd lng="en"><![CDATA[CRISPR/Cas system]]></kwd>
<kwd lng="en"><![CDATA[adaptive immune system]]></kwd>
</kwd-group>
</article-meta>
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