<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>1405-888X</journal-id>
<journal-title><![CDATA[TIP. Revista especializada en ciencias químico-biológicas]]></journal-title>
<abbrev-journal-title><![CDATA[TIP]]></abbrev-journal-title>
<issn>1405-888X</issn>
<publisher>
<publisher-name><![CDATA[Universidad Nacional Autónoma de México, Facultad de Estudios Superiores Zaragoza]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S1405-888X2016000200083</article-id>
<article-id pub-id-type="doi">10.1016/j.recqb.2016.06.001</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[Cytotoxic and genotoxic activity of phenolic fractions from Ulomoides dermestoides Fairmaire, 1893 (Coleoptera, Tenebrionidae), in HaCat cells]]></article-title>
<article-title xml:lang="es"><![CDATA[Actividad citotóxica y genotóxica de fracciones fenólicas de Ulomoides dermestoides Fairmaire, 1893 (Coleoptera, Tenebrionidae), en células HaCat]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Mendoza-Meza]]></surname>
<given-names><![CDATA[Dary Luz]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[España-Puccini]]></surname>
<given-names><![CDATA[Pierine]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad del Atlántico Facultad de Ciencias Básicas ]]></institution>
<addr-line><![CDATA[Puerto Colombia Departamento del Atlántico]]></addr-line>
<country>Colombia</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>08</month>
<year>2016</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>08</month>
<year>2016</year>
</pub-date>
<volume>19</volume>
<numero>2</numero>
<fpage>83</fpage>
<lpage>91</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S1405-888X2016000200083&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S1405-888X2016000200083&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S1405-888X2016000200083&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract: Ulomoides dermestoides (Fairmaire, 1893) is a beetle used in alternative medicine treatments in some South American countries. The objective of this study was to evaluate the cytotoxic and genotoxic effects of phenolic fractions (PF) from U. dermestoides . The PF were separated from crude extracts in acetone (PFAc) and ethanol (PFEtOH). The total phenolic content (TPC) was determined by Folin-Ciocalteu test. Volatile and semi-volatile compounds presents in crude extracts were identified by GC-MS; moreover, phenolic fractions were analyzed by HPLC-MS. The cellular viability, after exposition to phenolic fractions, was determined by Trypan blue exclusion test and MTT reduction assay on immortalized human keratinocyte cell line (HaCat); the degree of DNA damage was detected by alkaline comet-DNA assay. The TPC in PFAc and PFEtOH were: 11.34±0.88 mgGAE/g and 6.52±1.28 mgGAE/g, respectively (mean.dif: 4.951; p value = 0.0000). In both samples, HPLC-MS showed a pseudo-molecular ion [M-H]&#8722; at m/z 153, tentatively identified as protocatechuic acid. The results of cytotoxic assays suggest that the viability of HaCat cells depends on the concentration and exposure time of each treatment. Furthermore, the comet assay revealed moderate genotoxic effect after 48 hours of exposure to PFAc (40 to 160 &#956;g.mL&#8722;1); cytotoxic/genotoxic activity of this fraction could be related to the higher phenol contents.]]></p></abstract>
<abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen: Ulomoides dermestoides (Fairmaire, 1893), es un escarabajo usado en medicina alternativa en algunos países de Sudamérica. El objetivo de este estudio fue evaluar el efecto citotóxico y genotóxico de fracciones fenólicas (FF) de extractos de U. dermestoides . Las FF se separaron desde extractos acetónicos (FFAc) y etanólicos (FFEtOH). El contenido de fenoles totales (CFT) se determinó mediante ensayo de Folin-Ciocalteu. Compuestos volátiles y semi-volátiles, presentes en los extractos crudos, se identificaron mediante CG-EM; por otra parte, las FF se analizaron por HPLC-EM. La viabilidad celular, después de exposición a las FF se determinó mediante la prueba de exclusión con azul de tripano y el ensayo de reducción con MTT, usando la línea celular de queratinocitos humanos inmortalizados (HaCat); el grado de daño en el ADN se detectó mediante el ensayo de ADN-cometa alcalino. CFT en FFAc y FFEtOH fueron: 11,34±0,88 mgAGE/g y 6,52±1,28 mgAGE/g, respectivamente (dif.media: 4,951; p value = 0.0000). En ambas muestras, HPLC-EM mostró un ion pseudo-molecular [M&#8722;H]&#8722; a 153m/z, identificado tentativamente como ácido protocateuico. Los resultados de los ensayos de citotoxicidad sugieren que la viabilidad de células HaCat depende de la concentración y el tiempo de exposición a cada tratamiento. Además, el ensayo cometa reveló efecto genotóxico moderado después de 48 h de exposición a FFAc (40 a 160 &#956;g.mL&#8722;1). La actividad citotóxica/genotóxica de esta fracción podría estar relacionada con el contenido más alto de fenoles.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[beetles]]></kwd>
<kwd lng="en"><![CDATA[cell viability]]></kwd>
<kwd lng="en"><![CDATA[cytotoxicity]]></kwd>
<kwd lng="en"><![CDATA[genotoxicity]]></kwd>
<kwd lng="en"><![CDATA[phenolic fractions]]></kwd>
<kwd lng="en"><![CDATA[Ulomoides dermestoides]]></kwd>
<kwd lng="es"><![CDATA[escarabajos]]></kwd>
<kwd lng="es"><![CDATA[viabilidad celular]]></kwd>
<kwd lng="es"><![CDATA[citotoxicidad]]></kwd>
<kwd lng="es"><![CDATA[genotoxicidad]]></kwd>
<kwd lng="es"><![CDATA[fracciones fenólicas]]></kwd>
<kwd lng="es"><![CDATA[Ulomoides dermestoides]]></kwd>
</kwd-group>
</article-meta>
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