<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>1405-888X</journal-id>
<journal-title><![CDATA[TIP. Revista especializada en ciencias químico-biológicas]]></journal-title>
<abbrev-journal-title><![CDATA[TIP]]></abbrev-journal-title>
<issn>1405-888X</issn>
<publisher>
<publisher-name><![CDATA[Universidad Nacional Autónoma de México, Facultad de Estudios Superiores Zaragoza]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S1405-888X2009000100034</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Evidencia ultraestructural del nucléolo de Entamoeba histolytica]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Vázquez-Echeverría]]></surname>
<given-names><![CDATA[Citlali]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Zavala]]></surname>
<given-names><![CDATA[Guadalupe]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Chávez]]></surname>
<given-names><![CDATA[Bibiana]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Echeverría-Martínez]]></surname>
<given-names><![CDATA[Olga M.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Vázquez-Nin]]></surname>
<given-names><![CDATA[Gerardo H.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Segura-Valdez]]></surname>
<given-names><![CDATA[María de Lourdes]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Jiménez-García]]></surname>
<given-names><![CDATA[Luis F.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,UNAM Facultad de Ciencias Depto. de Biología Celular]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,UNAM Instituto de Biotecnología Unidad de Microscopía]]></institution>
<addr-line><![CDATA[Cuernavaca Morelos]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,IPN Centro de Investigación y de Estudios Avanzados Depto. de Patología Experimental]]></institution>
<addr-line><![CDATA[México D.F. ]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2009</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2009</year>
</pub-date>
<volume>12</volume>
<numero>1</numero>
<fpage>34</fpage>
<lpage>37</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S1405-888X2009000100034&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S1405-888X2009000100034&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S1405-888X2009000100034&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen: Aunque la estructura celular de Entamoeba histolytica se conoce con detalle, no se ha descrito la presencia de material nucleolar con el microscopio electrónico. En este trabajo utilizamos técnicas citoquímicas para microscopía de luz y electrónica para evidenciar la presencia del nucléolo en trofozoitos de esta amiba. Con la técnica de azul de toluidina para RNA y la técnica de impregnación argéntica para organizador nucleolar, se observa contraste de material intranuclear periférico. Con el microscopio electrónico, un material similar de naturaleza fibro-granular se contrasta también con las técnicas para ribonucleoproteínas y para organizador nucleolar. Una zona similar es negativa cuando se aplican técnicas para DNA. Estos resultados muestran evidencia ultraestructural de la presencia de un nucléolo periférico y anular en el núcleo de E. histolytica. Se sugiere que este organelo intranuclear es un caracter general de los eucariontes.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract: Although cell structure of Entamoeba histolytica is well known, the presence of nucleolar material has not been described with the electron microscope. Here we use light and electron microscopy cytochemical techniques to search for evidence1 of nucleolar material in this amoeba trophozoyte. Toluidine blue for RNA and silver staining for nucleolar organizer stain peripheral intranuclear material2. With the electron microscope, a3 similar material is fibro-granular and is contrasted with techniques for ribonucleoproteins and nucleolar organizer, but it is negative for DNA. These results show ultrastructural evidence for the presence of a peripheral and ringshaped nucleolus4 in the nucleus of E. histolytica. It is suggested that this intranuclear organelle is a general feature in eukaryotes.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Amiba]]></kwd>
<kwd lng="es"><![CDATA[Entamoeba histolytica]]></kwd>
<kwd lng="es"><![CDATA[núcleo]]></kwd>
<kwd lng="es"><![CDATA[nucléolo]]></kwd>
<kwd lng="es"><![CDATA[protistas]]></kwd>
<kwd lng="en"><![CDATA[Amoeba]]></kwd>
<kwd lng="en"><![CDATA[Entamoeba histolytica]]></kwd>
<kwd lng="en"><![CDATA[nucleus]]></kwd>
<kwd lng="en"><![CDATA[nucleolus]]></kwd>
<kwd lng="en"><![CDATA[protists]]></kwd>
</kwd-group>
</article-meta>
</front><back>
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