<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>1405-2768</journal-id>
<journal-title><![CDATA[Polibotánica]]></journal-title>
<abbrev-journal-title><![CDATA[Polibotánica]]></abbrev-journal-title>
<issn>1405-2768</issn>
<publisher>
<publisher-name><![CDATA[Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S1405-27682018000200241</article-id>
<article-id pub-id-type="doi">10.18387/polibotanica.46.16</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Generación de cultivos de raíces transformadas de la planta medicinal Bidens odorata Cav (Compositae) y análisis fitoquímico preliminar]]></article-title>
<article-title xml:lang="en"><![CDATA[Obtention of transformed roots cultures of the medicinal plant Bidens odorata Cav (Compositae) and preliminary phytochemical analysis]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Torres-García]]></surname>
<given-names><![CDATA[B.E.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Morales-Domínguez]]></surname>
<given-names><![CDATA[J.F.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Fraire-Velázquez]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Pérez-Molphe-Balch]]></surname>
<given-names><![CDATA[E.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Autónoma de Aguascalientes Departamento de Química Centro de Ciencias Básicas]]></institution>
<addr-line><![CDATA[Aguascalientes Ags]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad Autónoma de Zacatecas Laboratorio de Biología Integrativa de Plantas y Microorganismos Unidad Académica de Ciencias Biológicas]]></institution>
<addr-line><![CDATA[ Zacatecas]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2018</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2018</year>
</pub-date>
<numero>46</numero>
<fpage>241</fpage>
<lpage>257</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S1405-27682018000200241&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S1405-27682018000200241&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S1405-27682018000200241&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen: Se desarrolló un protocolo de propagación in vitro para Bidens odorata Cav. mediante el cultivo de segmentos nodales en medio de Murashige y Skoog adicionado con citocininas. Los mejores resultados fueron 5.5 ± 0.4 brotes por explante con benciladenina y 4.9 ± 0.3 con N6-(2-isopentenil)-adenina. También se evaluó su susceptibilidad a la transformación genética con tres cepas de Agrobacterium rhizogenes. La mejor cepa fue la A4/pESC4, a una densidad bacteriana de 107 UFC mL-1, que generó cultivos de raíces transformadas estables y de crecimiento activo, con una acumulación de biomasa de 4.8 veces el inóculo inicial a la tercera semana de cultivo. La verificación de la transformación se hizo histoquímicamente mediante la prueba de GUS, siendo positiva desde un 7 hasta un 82% de las raíces evaluadas; así como molecularmente, mediante la amplificación de los fragmentos correspondientes a los genes rolB, gus y nptII. El escrutinio fitoquímico realizado mediante cromatografía en capa fina reveló la capacidad que tienen las raíces transformadas para sintetizar compuestos de diversos tipos, sugiriendo la presencia de alcaloides, derivados de antraceno, aceites esenciales, flavonoides, saponinas, esteroides y triterpenos. Estos metabolitos secundarios están presentes en la parte aérea o en raíces de plantas de B. odorata, por lo que los resultados demostraron que las raíces transformadas pueden ser una opción viable para la obtención de los compuestos de interés farmacológico propios de esta especie.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract: An in vitro propagation protocol for Bidens odorata Cav. was developed by culturing nodal stem segments on Murashige and Skoog medium added with cytokinins. The best results were 5.5 ± 0.4 shoots per explant with benzyladenine, and 4.9 ± 0.3 with N6-(2-isopentenyl)-adenine. Their susceptibility to genetic transformation with three strains of Agrobacterium rhizogenes was also evaluated. The best was the A4/pESC4 strain, at a bacterial density of 107 CFU mL-1, which generated stable and active growth transformed roots cultures with a biomass accumulation of 4.8 times the initial inoculum at the third week of culture. The verification of the transformation was made histochemically by the GUS test, being positive from 7 to 82% of the evaluated roots; As well as molecularly, by amplifying the fragments corresponding to the rolB, gus and nptII genes. Phytochemical screening performed by thin layer chromatography revealed the ability of transformed roots to synthesize compounds of various types, suggesting the presence of alkaloids, anthracene derivatives, essential oils, flavonoids, saponins, steroids and triterpenes. These are present in the aerial part or roots of B. odorata, so that these results showed that the transformed roots may be a viable option for obtaining the compounds of pharmacological interest of this species.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Agrobacterium rhizogenes]]></kwd>
<kwd lng="es"><![CDATA[metabolitos secundarios]]></kwd>
<kwd lng="es"><![CDATA[raíces transformadas]]></kwd>
<kwd lng="es"><![CDATA[TLC]]></kwd>
<kwd lng="en"><![CDATA[Agrobacterium rhizogenes]]></kwd>
<kwd lng="en"><![CDATA[secondary metabolites]]></kwd>
<kwd lng="en"><![CDATA[TLC]]></kwd>
<kwd lng="en"><![CDATA[transformed roots]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<nlm-citation citation-type="">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Aqil]]></surname>
<given-names><![CDATA[F.]]></given-names>
</name>
<name>
<surname><![CDATA[Ahmad]]></surname>
<given-names><![CDATA[I.]]></given-names>
</name>
<name>
<surname><![CDATA[Owais]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Targeted Screening of Bioactive Plant Extracts and Phytocompounds Against Problematic Groups of Multidrug-Resistant Bacteria]]></article-title>
<source><![CDATA[Modern Phytomedicine: Turning Medicinal Plants into Drugs]]></source>
<year>2006</year>
</nlm-citation>
</ref>
<ref id="B2">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Astudillo-Vázquez]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[Dávalos Valle]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
<name>
<surname><![CDATA[De Jesús]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
<name>
<surname><![CDATA[Herrera]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
<name>
<surname><![CDATA[Navarrete]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Investigation of Alternanthera repens and Bidens odorata on gastrointestinal disease]]></article-title>
<source><![CDATA[Fitoterapia]]></source>
<year>2008</year>
</nlm-citation>
</ref>
<ref id="B3">
<nlm-citation citation-type="">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Bisht]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
<name>
<surname><![CDATA[Owais]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Venkatesan]]></surname>
<given-names><![CDATA[K.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Potential of Plant-Derived Products in the Treatment of Mycobacterial Infections]]></article-title>
<source><![CDATA[Modern Phytomedicine: Turning Medicinal Plants into Drugs]]></source>
<year>2006</year>
</nlm-citation>
</ref>
<ref id="B4">
<nlm-citation citation-type="">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Carlín]]></surname>
<given-names><![CDATA[A. P.]]></given-names>
</name>
<name>
<surname><![CDATA[Tafoya]]></surname>
<given-names><![CDATA[F.]]></given-names>
</name>
<name>
<surname><![CDATA[Alpuche Solís]]></surname>
<given-names><![CDATA[A. G.]]></given-names>
</name>
<name>
<surname><![CDATA[Pérez-Molphe-Balch]]></surname>
<given-names><![CDATA[E]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Effects of different culture media and conditions on biomass production of hairy root cultures in six Mexican cactus species]]></article-title>
<source><![CDATA[Vitro Cellular and Developmental Biology - Plant]]></source>
<year>2015</year>
</nlm-citation>
</ref>
<ref id="B5">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Chabaud]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Boisson]]></surname>
<given-names><![CDATA[D.A.]]></given-names>
</name>
<name>
<surname><![CDATA[Zhang]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
<name>
<surname><![CDATA[Taylor]]></surname>
<given-names><![CDATA[C.G.]]></given-names>
</name>
<name>
<surname><![CDATA[O.]]></surname>
<given-names><![CDATA[Yu]]></given-names>
</name>
<name>
<surname><![CDATA[Barker]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
</person-group>
<source><![CDATA[Agrobacterium rhizogenes-mediated root transformation]]></source>
<year>2006</year>
<volume>8</volume>
<publisher-name><![CDATA[Medicago Truncatula Handbook]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B6">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Chaudhuri]]></surname>
<given-names><![CDATA[K. N.]]></given-names>
</name>
<name>
<surname><![CDATA[Ghosh]]></surname>
<given-names><![CDATA[B.]]></given-names>
</name>
<name>
<surname><![CDATA[Tepfer]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
<name>
<surname><![CDATA[Jha]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Genetic transformation of Tylophora indica with Agrobacterium rhizogenes A4: Growth and tylophorine productivity in different transformed root clones]]></article-title>
<source><![CDATA[Plant Cell Reports]]></source>
<year>2005</year>
<volume>24</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>25-35</page-range></nlm-citation>
</ref>
<ref id="B7">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Deba]]></surname>
<given-names><![CDATA[F.]]></given-names>
</name>
<name>
<surname><![CDATA[Xuan]]></surname>
<given-names><![CDATA[T. D.]]></given-names>
</name>
<name>
<surname><![CDATA[Yasuda]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Tawata]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Herbicidal and fungicidal activities and identification of potential phytotoxins from Bidens pilosa L. var. radiata Scherff]]></article-title>
<source><![CDATA[Weed Biology and Management]]></source>
<year>2007</year>
</nlm-citation>
</ref>
<ref id="B8">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Dhakulkar]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Ganapathi]]></surname>
<given-names><![CDATA[T. R.]]></given-names>
</name>
<name>
<surname><![CDATA[Bhargava]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Bapat]]></surname>
<given-names><![CDATA[V. A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Induction of hairy roots in Gmelina arborea Roxb. and production of verbascoside in hairy roots]]></article-title>
<source><![CDATA[Plant Science]]></source>
<year>2005</year>
</nlm-citation>
</ref>
<ref id="B9">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Georgiev]]></surname>
<given-names><![CDATA[M.I.]]></given-names>
</name>
<name>
<surname><![CDATA[Pavlov]]></surname>
<given-names><![CDATA[A.I.]]></given-names>
</name>
<name>
<surname><![CDATA[Bley]]></surname>
<given-names><![CDATA[T.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Hairy root type plant in vitro systems as sources of bioactive substances]]></article-title>
<source><![CDATA[Applied Microbiology and Biotechnology]]></source>
<year>2007</year>
<volume>74</volume>
<page-range>1175-85</page-range></nlm-citation>
</ref>
<ref id="B10">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[González-Stuart]]></surname>
<given-names><![CDATA[A. E.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Use of medicinal plants in Monterrey, Mexico]]></article-title>
<source><![CDATA[Not.Sci.Biol]]></source>
<year>2010</year>
</nlm-citation>
</ref>
<ref id="B11">
<nlm-citation citation-type="">
<person-group person-group-type="author">
<name>
<surname><![CDATA[González]]></surname>
<given-names><![CDATA[E. M.]]></given-names>
</name>
<name>
<surname><![CDATA[López]]></surname>
<given-names><![CDATA[E. I. L.]]></given-names>
</name>
<name>
<surname><![CDATA[González]]></surname>
<given-names><![CDATA[E. M. S.]]></given-names>
</name>
<name>
<surname><![CDATA[Tena]]></surname>
<given-names><![CDATA[F. J. A.]]></given-names>
</name>
</person-group>
<source><![CDATA[Plantas medicinales del Estado de Durango y zonas aledañas]]></source>
<year>2004</year>
</nlm-citation>
</ref>
<ref id="B12">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Jacob]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[Malpathak]]></surname>
<given-names><![CDATA[N.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Manipulation of MS and B5 components for enhancement of growth and solasodine production in hairy root cultures of Solanum khasianum Clarke]]></article-title>
<source><![CDATA[Plant Cell, Tissue and Organ Culture]]></source>
<year>2005</year>
</nlm-citation>
</ref>
<ref id="B13">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Jofre-Garfias]]></surname>
<given-names><![CDATA[A. E.]]></given-names>
</name>
<name>
<surname><![CDATA[Villegas-Sepúlveda]]></surname>
<given-names><![CDATA[N.]]></given-names>
</name>
<name>
<surname><![CDATA[Cabrera-Ponce]]></surname>
<given-names><![CDATA[J. L.]]></given-names>
</name>
<name>
<surname><![CDATA[Adame-Alvarez]]></surname>
<given-names><![CDATA[R. M.]]></given-names>
</name>
<name>
<surname><![CDATA[Herrera-Estrella]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
<name>
<surname><![CDATA[Simpson]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Agrobacterium-mediated transformation of Amaranthus hypochondriacus: Light, and tissue-specific expression of a pea chlorophyll a/b-binding protein promoter]]></article-title>
<source><![CDATA[Plant Cell Reports]]></source>
<year>1997</year>
</nlm-citation>
</ref>
<ref id="B14">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Alonso-Castro]]></surname>
<given-names><![CDATA[Josabad]]></given-names>
</name>
<name>
<surname><![CDATA[Jose Maldonado-Miranda]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[Zarate-Martinez]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
<name>
<surname><![CDATA[Jacobo-Salcedo]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[Fernández-Galicia]]></surname>
<given-names><![CDATA[M. D. R.]]></given-names>
</name>
<name>
<surname><![CDATA[C.]]></surname>
<given-names><![CDATA[Alejandro]]></given-names>
</name>
<name>
<surname><![CDATA[Figueroa-Zuñiga]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
<name>
<surname><![CDATA[Carranza-Alvarez]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Medicinal plants used in the Huasteca Potosina, México]]></article-title>
<source><![CDATA[Journal of Ethnopharmacology]]></source>
<year>2012</year>
</nlm-citation>
</ref>
<ref id="B15">
<nlm-citation citation-type="">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Makhzoum]]></surname>
<given-names><![CDATA[A. B.]]></given-names>
</name>
<name>
<surname><![CDATA[Sharma]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
<name>
<surname><![CDATA[Bernards]]></surname>
<given-names><![CDATA[M. A.]]></given-names>
</name>
<name>
<surname><![CDATA[Trémouillaux-Guiller]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Hairy Roots: An Ideal Platform for Transgenic Plant Production and Other Promising Applications]]></article-title>
<source><![CDATA[Phytochemicals, Plant Growth, and the Environment]]></source>
<year>2013</year>
</nlm-citation>
</ref>
<ref id="B16">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Melody]]></surname>
<given-names><![CDATA[S. C.]]></given-names>
</name>
</person-group>
<source><![CDATA[Plant molecular biology - a laboratory manual]]></source>
<year>1997</year>
<publisher-name><![CDATA[Springer Berling Heidelberg]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B17">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Murashige]]></surname>
<given-names><![CDATA[T.]]></given-names>
</name>
<name>
<surname><![CDATA[Skoog]]></surname>
<given-names><![CDATA[F.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[A revised medium for rapid growth and bioassays with tobacco tissue cultures]]></article-title>
<source><![CDATA[Physiol Plant]]></source>
<year>1962</year>
</nlm-citation>
</ref>
<ref id="B18">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Murthy]]></surname>
<given-names><![CDATA[H. N.]]></given-names>
</name>
<name>
<surname><![CDATA[Dijkstra]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
<name>
<surname><![CDATA[Anthony]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
<name>
<surname><![CDATA[White]]></surname>
<given-names><![CDATA[D. A.]]></given-names>
</name>
<name>
<surname><![CDATA[Davey]]></surname>
<given-names><![CDATA[M. R.]]></given-names>
</name>
<name>
<surname><![CDATA[Power]]></surname>
<given-names><![CDATA[J. B.]]></given-names>
</name>
<name>
<surname><![CDATA[Paek]]></surname>
<given-names><![CDATA[K. Y.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Establishment of Withania somnifera hairy root cultures for the production of withanolide A]]></article-title>
<source><![CDATA[Journal of Integrative Plant Biology]]></source>
<year>2008</year>
</nlm-citation>
</ref>
<ref id="B19">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Njume]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
<name>
<surname><![CDATA[Gqaza]]></surname>
<given-names><![CDATA[B. M.]]></given-names>
</name>
<name>
<surname><![CDATA[Rozani]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
<name>
<surname><![CDATA[Goduka]]></surname>
<given-names><![CDATA[N. I.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Studies on bioactivity and secondary metabolites of crude extracts of Bidens pilosa L. (Asteraceae): A medicinal plant used in the Transkei region of South Africa]]></article-title>
<source><![CDATA[Pakistan Journal of Pharmaceutical Sciences]]></source>
<year>2016</year>
</nlm-citation>
</ref>
<ref id="B20">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Oliveira]]></surname>
<given-names><![CDATA[F. Q.]]></given-names>
</name>
<name>
<surname><![CDATA[Andrade-Neto]]></surname>
<given-names><![CDATA[V.]]></given-names>
</name>
<name>
<surname><![CDATA[Krettli]]></surname>
<given-names><![CDATA[A. U.]]></given-names>
</name>
<name>
<surname><![CDATA[Brandão]]></surname>
<given-names><![CDATA[M. G. L.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[New evidences of antimalarial activity of Bidens pilosa roots extract correlated with polyacetylene and flavonoids]]></article-title>
<source><![CDATA[Journal of Ethnopharmacology]]></source>
<year>2004</year>
</nlm-citation>
</ref>
<ref id="B21">
<nlm-citation citation-type="">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Palombo]]></surname>
<given-names><![CDATA[E. A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Traditional Plants and Herbal Remedies Used in the Treatment of Diarrheal Disease: Mode of Action, Quality, Efficacy, and Safety Considerations]]></article-title>
<source><![CDATA[Modern Phytomedicine: Turning Medicinal Plants into Drugs]]></source>
<year>2006</year>
</nlm-citation>
</ref>
<ref id="B22">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Pavlova]]></surname>
<given-names><![CDATA[O. A.]]></given-names>
</name>
<name>
<surname><![CDATA[Matveyeva]]></surname>
<given-names><![CDATA[T. V.]]></given-names>
</name>
<name>
<surname><![CDATA[Lutova]]></surname>
<given-names><![CDATA[L. A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[rol-Genes of Agrobacterium rhizogenes]]></article-title>
<source><![CDATA[Russian Journal of Genetics: Applied Research]]></source>
<year>2014</year>
</nlm-citation>
</ref>
<ref id="B23">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Quandt]]></surname>
<given-names><![CDATA[H. J.]]></given-names>
</name>
<name>
<surname><![CDATA[Puehler]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[Broer]]></surname>
<given-names><![CDATA[I.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Transgenic root nodules of Vicia hirsuta: a fast and efficient system for the study of gene expression in indeterminate-type nodules]]></article-title>
<source><![CDATA[MPMI]]></source>
<year>1993</year>
</nlm-citation>
</ref>
<ref id="B24">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Rout]]></surname>
<given-names><![CDATA[G. R.]]></given-names>
</name>
<name>
<surname><![CDATA[Samantaray]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Das]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[In vitro manipulation and propagation of medicinal plants]]></article-title>
<source><![CDATA[Biotechnology Advances]]></source>
<year>2000</year>
</nlm-citation>
</ref>
<ref id="B25">
<nlm-citation citation-type="">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Roychowdhury]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
<name>
<surname><![CDATA[Majumder]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[Jha]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Agrobacterium rhizogenes-mediated transformation in medicinal plants: Prospects and challenges]]></article-title>
<source><![CDATA[Biotechnology for Medicinal Plants: Micropropagation and Improvement]]></source>
<year>2013</year>
</nlm-citation>
</ref>
<ref id="B26">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Savka]]></surname>
<given-names><![CDATA[M. A.]]></given-names>
</name>
<name>
<surname><![CDATA[Ravillion]]></surname>
<given-names><![CDATA[B.]]></given-names>
</name>
<name>
<surname><![CDATA[Noel]]></surname>
<given-names><![CDATA[G. R.]]></given-names>
</name>
<name>
<surname><![CDATA[Farrand]]></surname>
<given-names><![CDATA[S. K.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Induction of hairy roots on cultivated soybean genotypes and their use to propagate the soybean cyst nematode]]></article-title>
<source><![CDATA[Phytopathology]]></source>
<year>1990</year>
</nlm-citation>
</ref>
<ref id="B27">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Silva]]></surname>
<given-names><![CDATA[F. L.]]></given-names>
</name>
<name>
<surname><![CDATA[Fischer]]></surname>
<given-names><![CDATA[D. C. H.]]></given-names>
</name>
<name>
<surname><![CDATA[Tavares]]></surname>
<given-names><![CDATA[J. F.]]></given-names>
</name>
<name>
<surname><![CDATA[Silva]]></surname>
<given-names><![CDATA[M. S.]]></given-names>
</name>
<name>
<surname><![CDATA[De Athayde-Filho]]></surname>
<given-names><![CDATA[P. F.]]></given-names>
</name>
<name>
<surname><![CDATA[Barbosa-Filho]]></surname>
<given-names><![CDATA[J. M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Compilation of secondary metabolites from Bidens pilosa L]]></article-title>
<source><![CDATA[Molecules]]></source>
<year>2011</year>
</nlm-citation>
</ref>
<ref id="B28">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Silva]]></surname>
<given-names><![CDATA[J. J.]]></given-names>
</name>
<name>
<surname><![CDATA[da]]></surname>
<given-names><![CDATA[C. D.]]></given-names>
</name>
<name>
<surname><![CDATA[Chavasco]]></surname>
<given-names><![CDATA[J. M.]]></given-names>
</name>
<name>
<surname><![CDATA[Cintra]]></surname>
<given-names><![CDATA[A. B. P.]]></given-names>
</name>
<name>
<surname><![CDATA[Silva]]></surname>
<given-names><![CDATA[C. B. P.]]></given-names>
</name>
<name>
<surname><![CDATA[da]]></surname>
<given-names><![CDATA[A. N.]]></given-names>
</name>
<name>
<surname><![CDATA[de]]></surname>
<given-names><![CDATA[J. K.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[In vitro screening antibacterial activity of Bidens pilosa Linné and Annona crassiflora mart. against oxacillin resistant Staphylococcus aureus (ORSA) from the aerial environment at the dental clinic]]></article-title>
<source><![CDATA[Revista Do Instituto de Medicina Tropical de São Paulo]]></source>
<year>2014</year>
</nlm-citation>
</ref>
<ref id="B29">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Somashekar]]></surname>
<given-names><![CDATA[M. N.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[A Review on anthracene and its derivatives: applications]]></article-title>
<source><![CDATA[Research &amp; Reviews: Journal of Chemistry]]></source>
<year>2016</year>
<volume>5</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>45-52</page-range></nlm-citation>
</ref>
<ref id="B30">
<nlm-citation citation-type="">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Stomp]]></surname>
<given-names><![CDATA[A. M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Histochemical localization of &#402;-glucuronidase]]></article-title>
<source><![CDATA[GUS Protocols: Using the GUS Gene as a Reporter of Gene Expression]]></source>
<year>1992</year>
</nlm-citation>
</ref>
<ref id="B31">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Sutton]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Measurement of microbial cells by optical density]]></article-title>
<source><![CDATA[J. Validation Techn]]></source>
<year>2011</year>
</nlm-citation>
</ref>
<ref id="B32">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Uddin]]></surname>
<given-names><![CDATA[M. R.]]></given-names>
</name>
<name>
<surname><![CDATA[Li]]></surname>
<given-names><![CDATA[X.]]></given-names>
</name>
<name>
<surname><![CDATA[Won]]></surname>
<given-names><![CDATA[O. J.]]></given-names>
</name>
<name>
<surname><![CDATA[Park]]></surname>
<given-names><![CDATA[S. U.]]></given-names>
</name>
<name>
<surname><![CDATA[Pyon]]></surname>
<given-names><![CDATA[J. Y.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Herbicidal activity of phenolic compounds from hairy root cultures of Fagopyrum tataricum]]></article-title>
<source><![CDATA[Weed Research]]></source>
<year>2013</year>
<volume>52</volume>
<page-range>25-33</page-range></nlm-citation>
</ref>
<ref id="B33">
<nlm-citation citation-type="">
<collab>UNAM</collab>
<source><![CDATA[Biblioteca Digital de la Medicina Tradicional Mexicana]]></source>
<year>2009</year>
</nlm-citation>
</ref>
<ref id="B34">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Veena]]></surname>
<given-names><![CDATA[V.]]></given-names>
</name>
<name>
<surname><![CDATA[C. G.]]></surname>
<given-names><![CDATA[Taylor]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Agrobacterium rhizogenes : recent developments and promising applications]]></article-title>
<source><![CDATA[Vitro Cellular And Developmental Biology]]></source>
<year>2007</year>
<volume>43</volume>
<numero>5</numero>
<issue>5</issue>
<page-range>383-403</page-range></nlm-citation>
</ref>
<ref id="B35">
<nlm-citation citation-type="">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Wagner]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
<name>
<surname><![CDATA[Bladt]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
</person-group>
<source><![CDATA[Plant Drug Analysis]]></source>
<year>1996</year>
</nlm-citation>
</ref>
<ref id="B36">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Zahara]]></surname>
<given-names><![CDATA[K.]]></given-names>
</name>
<name>
<surname><![CDATA[Bibi]]></surname>
<given-names><![CDATA[Y.]]></given-names>
</name>
<name>
<surname><![CDATA[Tabassum]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Mudrikah, Bashir]]></surname>
<given-names><![CDATA[T.]]></given-names>
</name>
<name>
<surname><![CDATA[Haider]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Ajmal]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[A review on pharmacological properties of Bidens biternata: A potential nutraceutical]]></article-title>
<source><![CDATA[Asian Pacific Journal of Tropical Disease]]></source>
<year>2015</year>
<volume>5</volume>
<numero>8</numero>
<issue>8</issue>
<page-range>595-9</page-range></nlm-citation>
</ref>
<ref id="B37">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Zavala-Mendoza]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
<name>
<surname><![CDATA[Alarcon-Aguilar]]></surname>
<given-names><![CDATA[F. J.]]></given-names>
</name>
<name>
<surname><![CDATA[Pérez-Gutierrez]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Carmen Escobar-Villanueva]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Zavala-Sánchez]]></surname>
<given-names><![CDATA[M. A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Composition and antidiarrheal activity of bidens odorata cav]]></article-title>
<source><![CDATA[Evidence-Based Complementary and Alternative Medicine]]></source>
<year>2013</year>
</nlm-citation>
</ref>
</ref-list>
</back>
</article>
