<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0301-696X</journal-id>
<journal-title><![CDATA[Revista de sanidad militar]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. sanid. mil.]]></abbrev-journal-title>
<issn>0301-696X</issn>
<publisher>
<publisher-name><![CDATA[Secretaría de la Defensa Nacional, Dirección General de Sanidad]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0301-696X2018000200077</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Detección de células madre mesenquimales de origen urinario mediante espectroscopia de impedancia]]></article-title>
<article-title xml:lang="en"><![CDATA[Detection of mesenchymal stem cells of urinary origin using impedance spectroscopy]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Silva-Escobedo]]></surname>
<given-names><![CDATA[Jesús Gabriel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[González-Ruiz]]></surname>
<given-names><![CDATA[Margarita Nora]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Orihuela-Servín]]></surname>
<given-names><![CDATA[Rodolfo Mario]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Bahena-Carrera]]></surname>
<given-names><![CDATA[Lucio]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Secretaría de la Defensa Nacional Escuela Militar de Graduados de Sanidad Centro Militar de Ciencias de la Salud]]></institution>
<addr-line><![CDATA[Ciudad de México ]]></addr-line>
<country>México</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Secretaría de la Defensa Nacional Escuela Militar de Graduados de Sanidad Centro Militar de Ciencias de la Salud]]></institution>
<addr-line><![CDATA[Ciudad de México ]]></addr-line>
<country>México</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Hospital Central Militar Servicio de Nefrología ]]></institution>
<addr-line><![CDATA[Ciudad de México ]]></addr-line>
<country>México</country>
</aff>
<aff id="Af4">
<institution><![CDATA[,Hospital Central Militar Servicio de Nefrología ]]></institution>
<addr-line><![CDATA[Ciudad de México ]]></addr-line>
<country>México</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>04</month>
<year>2018</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>04</month>
<year>2018</year>
</pub-date>
<volume>72</volume>
<numero>2</numero>
<fpage>77</fpage>
<lpage>83</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0301-696X2018000200077&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S0301-696X2018000200077&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S0301-696X2018000200077&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen  Introducción La medicina regenerativa es una rama de la medicina relacionada con el empleo de células madre (CM) para aprovechar su capacidad para reemplazar o regenerar células, tejidos u órganos, para restaurar una función dañada por alguna causa, incluyendo defectos congénitos, trauma y envejecimiento. Las técnicas de obtención, aislamiento e inmunotipificación (como son la citometría de flujo, pruebas de inmunofluorescencia y microscopia de fluorescencia, así como técnicas de biología molecular) actualmente requieren infraestructura y personal especializado, además de ser costosas y de difícil acceso.  Objetivo Proponer y evaluar una técnica para la detección y aislamiento de células madre en muestras de orina mediante el empleo de un biosensor, con el uso de espectroscopia de impedancia asistida con nanosondas.  Material y métodos Las células obtenidas fueron cultivadas y luego marcadas con nanosondas específicas para marcadores de células madre indiferenciadas de origen mesenquimal.  Resultados Los resultados iniciales de la espectroscopia de impedancia eléctrica, donde se compararon células sin marcaje con nanosondas y células marcadas con nanosondas, no son concluyentes; sin embargo, tienen una tendencia a mostrar algunas diferencias entre ambas condiciones. Además, se realizaron análisis de microscopia de fluorescencia y multifotónica para corroborar la presencia de células madre mesenquimales.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract  Introduction: Regenerative medicine is a branch of medicine related to the use of stem cells (SC) to take advantage of their capacity to replace or regenerate cells, tissues or organs to restore a function damaged by some cause, including congenital defects, trauma and aging. The techniques for obtaining, isolating and immunotyping (such as fl ow cytometry, immunofl uorescence and fl uorescence microscopy tests, as well as molecular biology techniques) currently require specialized infrastructure and personnel, and they are expensive and diffi cult to access.  Objective: To propose and evaluate a technique for the detection and isolation of stem cells in urine samples by using a biosensor with impedance spectroscopy assisted by nanoprobes. Material and methods: The cells obtained were cultured and then labeled with specifi c nanoprobes for markers of undifferentiated stem cells of mesenchymal origin.  Results: The initial results of electrical impedance spectroscopy, where cells without labeling were compared with cells labeled with nanoprobes, are inconclusive; however, they tend to show some differences between both conditions. Additionally, fl uorescence and multiphoton microscopy analysis were carried out, as well as fl ow cytometry, to corroborate the presence of mesenchymal stem cells.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Célula madre]]></kwd>
<kwd lng="es"><![CDATA[orina]]></kwd>
<kwd lng="es"><![CDATA[medicina regenerativa]]></kwd>
<kwd lng="es"><![CDATA[terapia celular]]></kwd>
<kwd lng="en"><![CDATA[Stem cell]]></kwd>
<kwd lng="en"><![CDATA[urine]]></kwd>
<kwd lng="en"><![CDATA[regenerative medicine]]></kwd>
<kwd lng="en"><![CDATA[cell therapy]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<label>1</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Greenwood]]></surname>
<given-names><![CDATA[HL]]></given-names>
</name>
<name>
<surname><![CDATA[Singer]]></surname>
<given-names><![CDATA[PA]]></given-names>
</name>
<name>
<surname><![CDATA[Downey]]></surname>
<given-names><![CDATA[GP]]></given-names>
</name>
<name>
<surname><![CDATA[Martin]]></surname>
<given-names><![CDATA[DK]]></given-names>
</name>
<name>
<surname><![CDATA[Thorsteinsdóttir]]></surname>
<given-names><![CDATA[H]]></given-names>
</name>
<name>
<surname><![CDATA[Daar]]></surname>
<given-names><![CDATA[AS]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Regenerative medicine and the developing world]]></article-title>
<source><![CDATA[PLoS Med]]></source>
<year>2006</year>
<volume>3</volume>
<numero>9</numero>
<issue>9</issue>
</nlm-citation>
</ref>
<ref id="B2">
<label>2</label><nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Grenier]]></surname>
<given-names><![CDATA[G]]></given-names>
</name>
<name>
<surname><![CDATA[Rudnicki]]></surname>
<given-names><![CDATA[MA]]></given-names>
</name>
</person-group>
<source><![CDATA[Stem cells]]></source>
<year>2006</year>
<publisher-loc><![CDATA[NY ]]></publisher-loc>
<publisher-name><![CDATA[Springer]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B3">
<label>3</label><nlm-citation citation-type="">
<source><![CDATA[Células madre vs. insuficiencia renal crónica]]></source>
<year></year>
</nlm-citation>
</ref>
<ref id="B4">
<label>4</label><nlm-citation citation-type="">
<collab>Instituto Nacional de Salud Pública</collab>
<source><![CDATA[Encuesta Nacional de Salud y Nutrición]]></source>
<year></year>
</nlm-citation>
</ref>
<ref id="B5">
<label>5</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Espinosa-Cuevas]]></surname>
<given-names><![CDATA[MA]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Enfermedad renal]]></article-title>
<source><![CDATA[Gac Med Mex]]></source>
<year>2016</year>
<volume>152</volume>
<numero>^s1</numero>
<issue>^s1</issue>
<supplement>1</supplement>
<page-range>90-6</page-range></nlm-citation>
</ref>
<ref id="B6">
<label>6</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Bodin]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Bharadwaj]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Wu]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Gatenholm]]></surname>
<given-names><![CDATA[P]]></given-names>
</name>
<name>
<surname><![CDATA[Atala]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Zhang]]></surname>
<given-names><![CDATA[Y]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Tissue-engineered conduit using urine-derived stem cells seeded bacterial cellulose polymer in urinary reconstruction and diversion]]></article-title>
<source><![CDATA[Biomaterials]]></source>
<year>2010</year>
<volume>31</volume>
<numero>34</numero>
<issue>34</issue>
<page-range>8889-901</page-range></nlm-citation>
</ref>
<ref id="B7">
<label>7</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Wu]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Liu]]></surname>
<given-names><![CDATA[Y]]></given-names>
</name>
<name>
<surname><![CDATA[Bharadwaj]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Atala]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Zhang]]></surname>
<given-names><![CDATA[Y]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Human urine-derived stem cells seeded in a modified 3D porous small intestinal submucosa scaffold for urethral tissue engineering]]></article-title>
<source><![CDATA[Biomaterials]]></source>
<year>2011</year>
<volume>32</volume>
<numero>5</numero>
<issue>5</issue>
<page-range>1317-26</page-range></nlm-citation>
</ref>
<ref id="B8">
<label>8</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Bharadwaj]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Liu]]></surname>
<given-names><![CDATA[G]]></given-names>
</name>
<name>
<surname><![CDATA[Shi]]></surname>
<given-names><![CDATA[Y]]></given-names>
</name>
<name>
<surname><![CDATA[Markert]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
<name>
<surname><![CDATA[Andersson]]></surname>
<given-names><![CDATA[KE]]></given-names>
</name>
<name>
<surname><![CDATA[Atala]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Characterization of urine-derived stem cells obtained from upper urinary tract for use in cell-based urological tissue engineering]]></article-title>
<source><![CDATA[Tissue Eng]]></source>
<year>2011</year>
<volume>17</volume>
<numero>15-16</numero>
<issue>15-16</issue>
<page-range>2123-32</page-range></nlm-citation>
</ref>
<ref id="B9">
<label>9</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Bharadwaj]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Liu]]></surname>
<given-names><![CDATA[G]]></given-names>
</name>
<name>
<surname><![CDATA[Shi]]></surname>
<given-names><![CDATA[Y]]></given-names>
</name>
<name>
<surname><![CDATA[Wu]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
<name>
<surname><![CDATA[Yang]]></surname>
<given-names><![CDATA[B]]></given-names>
</name>
<name>
<surname><![CDATA[He]]></surname>
<given-names><![CDATA[T]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Multipotential differentiation of human urine-derived stem cells: potential for therapeutic applications in urology]]></article-title>
<source><![CDATA[Stem Cells]]></source>
<year>2013</year>
<volume>31</volume>
<numero>9</numero>
<issue>9</issue>
<page-range>1840-56</page-range></nlm-citation>
</ref>
<ref id="B10">
<label>10</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Zhang]]></surname>
<given-names><![CDATA[Y Y]]></given-names>
</name>
<name>
<surname><![CDATA[Ludwikowski]]></surname>
<given-names><![CDATA[B]]></given-names>
</name>
<name>
<surname><![CDATA[Hurst]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
<name>
<surname><![CDATA[Frey]]></surname>
<given-names><![CDATA[P]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Expansion and long-term culture of differentiated normal rat urothelial cells in vitro]]></article-title>
<source><![CDATA[Vitro Cell Dev Biol Anim]]></source>
<year>2001</year>
<volume>37</volume>
<numero>7</numero>
<issue>7</issue>
<page-range>419-29</page-range></nlm-citation>
</ref>
<ref id="B11">
<label>11</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[González-Díaz]]></surname>
<given-names><![CDATA[CA]]></given-names>
</name>
<name>
<surname><![CDATA[Cleva Villanueva-López]]></surname>
<given-names><![CDATA[G]]></given-names>
</name>
<name>
<surname><![CDATA[Takao Kaneko-Wada]]></surname>
<given-names><![CDATA[FJ]]></given-names>
</name>
<name>
<surname><![CDATA[Sacristán-Rock]]></surname>
<given-names><![CDATA[E]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Análisis biomédico de la espectroscopia de impedancia: Una nueva herramienta en cuidado crítico]]></article-title>
<source><![CDATA[Rev Sanid Milit Mex]]></source>
<year>2004</year>
<volume>58</volume>
<numero>5</numero>
<issue>5</issue>
<page-range>404-10</page-range></nlm-citation>
</ref>
<ref id="B12">
<label>12</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Han]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Yang]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
<name>
<surname><![CDATA[Frazier]]></surname>
<given-names><![CDATA[AB]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Quantification of the heterogeneity in breast cancer cell lines using whole-cell impedance spectroscopy]]></article-title>
<source><![CDATA[Clin Cancer Res]]></source>
<year>2007</year>
<volume>13</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>139-43</page-range></nlm-citation>
</ref>
<ref id="B13">
<label>13</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Silva]]></surname>
<given-names><![CDATA[JG]]></given-names>
</name>
<name>
<surname><![CDATA[Cárdenas]]></surname>
<given-names><![CDATA[RA]]></given-names>
</name>
<name>
<surname><![CDATA[Quiróz]]></surname>
<given-names><![CDATA[AR]]></given-names>
</name>
<name>
<surname><![CDATA[Sánchez]]></surname>
<given-names><![CDATA[V]]></given-names>
</name>
<name>
<surname><![CDATA[Lozano]]></surname>
<given-names><![CDATA[LM]]></given-names>
</name>
<name>
<surname><![CDATA[Pérez]]></surname>
<given-names><![CDATA[NM]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Impedance spectroscopy assisted by magnetic nanoparticles as a potential biosensor principle for breast cancer cells in suspension]]></article-title>
<source><![CDATA[Physiol Meas]]></source>
<year>2014</year>
<volume>35</volume>
<numero>6</numero>
<issue>6</issue>
<page-range>931-41</page-range></nlm-citation>
</ref>
<ref id="B14">
<label>14</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Zhang]]></surname>
<given-names><![CDATA[Y Y]]></given-names>
</name>
<name>
<surname><![CDATA[Frey]]></surname>
<given-names><![CDATA[P]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Growth of cultured human urothelial cells into stratified urothelial sheet suitable for autografts]]></article-title>
<source><![CDATA[Adv Exp Med Biol]]></source>
<year>2003</year>
<volume>539</volume>
<page-range>907-20</page-range></nlm-citation>
</ref>
</ref-list>
</back>
</article>
