<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0188-6266</journal-id>
<journal-title><![CDATA[Acta universitaria]]></journal-title>
<abbrev-journal-title><![CDATA[Acta univ]]></abbrev-journal-title>
<issn>0188-6266</issn>
<publisher>
<publisher-name><![CDATA[Universidad de Guanajuato, Dirección de Investigación y Posgrado]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0188-62662022000100133</article-id>
<article-id pub-id-type="doi">10.15174/au.2022.3435</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[El operón thnRDE de B. thuringiensis le brinda inmunidad a Lactococcus lactis contra thurincina H]]></article-title>
<article-title xml:lang="en"><![CDATA[The thnRDE operon from B. thuringiensis provides immunity to Lactococcus lactis against thurincin H]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Barboza-Corona]]></surname>
<given-names><![CDATA[José E.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Núñez Valle]]></surname>
<given-names><![CDATA[Mario Alberto]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Velázquez Juárez]]></surname>
<given-names><![CDATA[Gilberto]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Casados Vázquez]]></surname>
<given-names><![CDATA[Luz Edith]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad de Guanajuato Departamento de Alimentos ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad de Guadalajara Centro Universitario de Ciencias Exactas e Ingenierías ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2022</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2022</year>
</pub-date>
<volume>32</volume>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0188-62662022000100133&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S0188-62662022000100133&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S0188-62662022000100133&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen Lactococcus lactis es una bacteria ácido-láctica de gran importancia para la industria de los alimentos. Es una cepa segura, y por tanto se han diseñado sistemas para la expresión de proteínas recombinantes de interés biotecnológico. Dentro de las proteínas con importancia biotecnológica se encuentra la bacteriocina thurincina H, esta tiene muchas características favorables para ser empleada como bioconservador en alimentos. Se ha reportado que necesita al menos nueve genes para su biosíntesis, dentro de estos están los genes thnRDE que brindan inmunidad a la cepa productora nativa. El objetivo de este trabajo fue integrar estos tres genes a la cepa L. lactis para hacerla resistente a thurincina H y poder producirla en un futuro. Para lograrlo, se clonaron los marcos abiertos de lectura (ORF, por sus siglas en inglés) de los genes thnRDE en un vector para L. lactis, y se transformaron en la cepa sensible. Se logró obtener una cepa de L. lactis resistente a thurincina H que podrá ser empleada para su producción heteróloga segura.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract Lactococcus lactis is a lactic acid bacterium of great importance for the food industry. Due to L. lactis safety status, systems have been designed for the expression of recombinant proteins of biotechnological interest. A peptide with biotechnological importance is the bacteriocin thurincin H, which has many favorable characteristics to be used as a bioconservative in food. It has been reported that it needs at least nine genes for its biosynthesis, within these, the thnRDE genes provide immunity to the native producer strain. The objective of this work was to integrate these genes into the L. lactis strain to make it resistant to thurincin H and to be able to produce it in the future. To achieve this, the thnRDE genes were cloned into a vector for L. lactis and transformed into the sensitive strain. It was possible to obtain a strain of L. lactis resistant to thurincin H that can be used for its safe heterologous production.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Inmunidad]]></kwd>
<kwd lng="es"><![CDATA[thurincina H]]></kwd>
<kwd lng="es"><![CDATA[Lactococcus lactis]]></kwd>
<kwd lng="en"><![CDATA[Immunity]]></kwd>
<kwd lng="en"><![CDATA[thurincin H]]></kwd>
<kwd lng="en"><![CDATA[Lactococcus lactis]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Birnboim]]></surname>
<given-names><![CDATA[H. C.]]></given-names>
</name>
<name>
<surname><![CDATA[Doly]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[A rapid alkaline extraction procedure for screening recombinant plasmid DNA]]></article-title>
<source><![CDATA[Nucleic Acids Research]]></source>
<year>1979</year>
<volume>7</volume>
<numero>6</numero>
<issue>6</issue>
<page-range>1513-23</page-range></nlm-citation>
</ref>
<ref id="B2">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Casados-Vázquez]]></surname>
<given-names><![CDATA[L. E.]]></given-names>
</name>
<name>
<surname><![CDATA[Bideshi]]></surname>
<given-names><![CDATA[D. K.]]></given-names>
</name>
<name>
<surname><![CDATA[Barboza-Corona]]></surname>
<given-names><![CDATA[J. E.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[The thnR gene is a negative transcription regulator of the thurincin H genetic cassette in Bacillus thuringiensis subsp. morrisoni]]></article-title>
<source><![CDATA[Archives of Microbiology]]></source>
<year>2017</year>
<volume>199</volume>
<page-range>385-90</page-range></nlm-citation>
</ref>
<ref id="B3">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Casados-Vázquez]]></surname>
<given-names><![CDATA[L. E.]]></given-names>
</name>
<name>
<surname><![CDATA[Bideshi]]></surname>
<given-names><![CDATA[D. K.]]></given-names>
</name>
<name>
<surname><![CDATA[Barboza-Corona]]></surname>
<given-names><![CDATA[J. E.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Regulator ThnR and the ThnDE ABC transporter proteins confer autoimmunity to thurincin H in Bacillus thuringiensis]]></article-title>
<source><![CDATA[Antonie van Leeuwenhoek]]></source>
<year>2018</year>
<volume>111</volume>
<page-range>2349-60</page-range></nlm-citation>
</ref>
<ref id="B4">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Cavanagh]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
<name>
<surname><![CDATA[Fitzgerald]]></surname>
<given-names><![CDATA[G. F.]]></given-names>
</name>
<name>
<surname><![CDATA[McAuliffe]]></surname>
<given-names><![CDATA[O.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[From field to fermentation: The origins of Lactococcus lactis and its domestication to the dairy environment]]></article-title>
<source><![CDATA[Food microbiology]]></source>
<year>2015</year>
<volume>47</volume>
<page-range>45-61</page-range></nlm-citation>
</ref>
<ref id="B5">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Commane]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
<name>
<surname><![CDATA[Hughes]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
<name>
<surname><![CDATA[Shortt]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
<name>
<surname><![CDATA[Rowland]]></surname>
<given-names><![CDATA[I.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[The potential mechanisms involved in the anti-carcinogenic action of probiotics]]></article-title>
<source><![CDATA[Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis]]></source>
<year>2005</year>
<volume>591</volume>
<numero>1-2</numero>
<issue>1-2</issue>
<page-range>276-89</page-range></nlm-citation>
</ref>
<ref id="B6">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Han]]></surname>
<given-names><![CDATA[E. J.]]></given-names>
</name>
<name>
<surname><![CDATA[Lee]]></surname>
<given-names><![CDATA[N]]></given-names>
</name>
<name>
<surname><![CDATA[Choi]]></surname>
<given-names><![CDATA[S. Y.]]></given-names>
</name>
<name>
<surname><![CDATA[Paik]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Bacteriocin KC24 produced by Lactococcus lactis KC24 from kimchi and its antilisterial effect in UHT milk]]></article-title>
<source><![CDATA[Journal of Dairy Science]]></source>
<year>2013</year>
<volume>96</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>101-4</page-range></nlm-citation>
</ref>
<ref id="B7">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Hansen]]></surname>
<given-names><![CDATA[M. E.]]></given-names>
</name>
<name>
<surname><![CDATA[Wangari]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
<name>
<surname><![CDATA[Hansen]]></surname>
<given-names><![CDATA[E. B.]]></given-names>
</name>
<name>
<surname><![CDATA[Mijakovic]]></surname>
<given-names><![CDATA[I.]]></given-names>
</name>
<name>
<surname><![CDATA[Jensen]]></surname>
<given-names><![CDATA[P. R.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Engineering of Bacillus subtilis 168 for increased nisin resistance]]></article-title>
<source><![CDATA[Applied and Environmental Microbiology]]></source>
<year>2009</year>
<volume>75</volume>
<numero>21</numero>
<issue>21</issue>
<page-range>6688-95</page-range></nlm-citation>
</ref>
<ref id="B8">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Jørgensen]]></surname>
<given-names><![CDATA[C. M.]]></given-names>
</name>
<name>
<surname><![CDATA[Vrang]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[Madsen]]></surname>
<given-names><![CDATA[S. M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Recombinant protein expression in Lactococcus lactis using the P170 expression system]]></article-title>
<source><![CDATA[FEMS Microbiology Letters]]></source>
<year>2014</year>
<volume>351</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>170-8</page-range></nlm-citation>
</ref>
<ref id="B9">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Kimoto]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
<name>
<surname><![CDATA[Mizumachi]]></surname>
<given-names><![CDATA[K.]]></given-names>
</name>
<name>
<surname><![CDATA[Okamoto]]></surname>
<given-names><![CDATA[T.]]></given-names>
</name>
<name>
<surname><![CDATA[Kurisaki]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[New Lactococcus strain with immunomodulatory activity: Enhancement of Th1&#8208;type immune response]]></article-title>
<source><![CDATA[Microbiology and Immunology]]></source>
<year>2004</year>
<volume>48</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>75-82</page-range></nlm-citation>
</ref>
<ref id="B10">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Kuipers]]></surname>
<given-names><![CDATA[O. P.]]></given-names>
</name>
<name>
<surname><![CDATA[Beerthuyzen]]></surname>
<given-names><![CDATA[M. M.]]></given-names>
</name>
<name>
<surname><![CDATA[de Ruyter]]></surname>
<given-names><![CDATA[P. G.]]></given-names>
</name>
<name>
<surname><![CDATA[Luesink]]></surname>
<given-names><![CDATA[E. J.]]></given-names>
</name>
<name>
<surname><![CDATA[de Vos]]></surname>
<given-names><![CDATA[W. M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Autoregulation of nisin biosynthesis in Lactococcus lactis by signal transduction]]></article-title>
<source><![CDATA[Journal of Biological Chemistry]]></source>
<year>1995</year>
<volume>270</volume>
<numero>45</numero>
<issue>45</issue>
<page-range>27299-304</page-range></nlm-citation>
</ref>
<ref id="B11">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Lee]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
<name>
<surname><![CDATA[Churey]]></surname>
<given-names><![CDATA[J. J.]]></given-names>
</name>
<name>
<surname><![CDATA[Worobo]]></surname>
<given-names><![CDATA[R. W.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Biosynthesis and transcriptional analysis of thurincin H, a tandem repeated bacteriocin genetic locus, produced by Bacillus thuringiensis SF361]]></article-title>
<source><![CDATA[FEMS Microbiology Letters]]></source>
<year>2009</year>
<volume>299</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>205-13</page-range></nlm-citation>
</ref>
<ref id="B12">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Mierau]]></surname>
<given-names><![CDATA[I.]]></given-names>
</name>
<name>
<surname><![CDATA[Kleerebezem]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis]]></article-title>
<source><![CDATA[Applied Microbiology and Biotechnology]]></source>
<year>2005</year>
<volume>68</volume>
<page-range>705-17</page-range></nlm-citation>
</ref>
<ref id="B13">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Morello]]></surname>
<given-names><![CDATA[E.]]></given-names>
</name>
<name>
<surname><![CDATA[Bermudez-Humarán]]></surname>
<given-names><![CDATA[L. G.]]></given-names>
</name>
<name>
<surname><![CDATA[Llull]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
<name>
<surname><![CDATA[Solé]]></surname>
<given-names><![CDATA[V.]]></given-names>
</name>
<name>
<surname><![CDATA[Miraglio]]></surname>
<given-names><![CDATA[N.]]></given-names>
</name>
<name>
<surname><![CDATA[Langella]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
<name>
<surname><![CDATA[Poquet]]></surname>
<given-names><![CDATA[I.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Lactococcus lactis, an efficient cell factory for recombinant protein production and secretion]]></article-title>
<source><![CDATA[Journal of Molecular Microbiology and Biotechnology]]></source>
<year>2008</year>
<volume>14</volume>
<numero>1-3</numero>
<issue>1-3</issue>
<page-range>48-58</page-range></nlm-citation>
</ref>
<ref id="B14">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Oros-Flores]]></surname>
<given-names><![CDATA[Z. S.]]></given-names>
</name>
<name>
<surname><![CDATA[Casados-Vázquez]]></surname>
<given-names><![CDATA[L. E.]]></given-names>
</name>
<name>
<surname><![CDATA[Bideshi]]></surname>
<given-names><![CDATA[D. K.]]></given-names>
</name>
<name>
<surname><![CDATA[Salcedo-Hernández]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
<name>
<surname><![CDATA[Barboza-Corona]]></surname>
<given-names><![CDATA[J. E.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Co-synthesis of kenyacin 404 and heterologous thurincin H enhances the antibacterial activity of Bacillus thuringiensis]]></article-title>
<source><![CDATA[Biotechnology Letters]]></source>
<year>2018</year>
<volume>40</volume>
<page-range>1531-40</page-range></nlm-citation>
</ref>
<ref id="B15">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Platteeuw]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
<name>
<surname><![CDATA[van Alen-Boerrigter]]></surname>
<given-names><![CDATA[I.]]></given-names>
</name>
<name>
<surname><![CDATA[van Schalkwijk]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[de Vos]]></surname>
<given-names><![CDATA[W. M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Food-grade cloning and expression system for Lactococcus lactis]]></article-title>
<source><![CDATA[Applied and Environmental Microbiology]]></source>
<year>1996</year>
<volume>62</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>1008-13</page-range></nlm-citation>
</ref>
<ref id="B16">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Rezac]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Kok]]></surname>
<given-names><![CDATA[C. R.]]></given-names>
</name>
<name>
<surname><![CDATA[Heermann]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Hutkins]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Fermented foods as a dietary source of live organisms]]></article-title>
<source><![CDATA[Frontiers in Microbiology]]></source>
<year>2018</year>
<volume>9</volume>
</nlm-citation>
</ref>
<ref id="B17">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Song]]></surname>
<given-names><![CDATA[A. A.]]></given-names>
</name>
<name>
<surname><![CDATA[In]]></surname>
<given-names><![CDATA[L. L. A.]]></given-names>
</name>
<name>
<surname><![CDATA[Lim]]></surname>
<given-names><![CDATA[S. H. E.]]></given-names>
</name>
<name>
<surname><![CDATA[Rahim]]></surname>
<given-names><![CDATA[R. A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[A review on Lactococcus lactis: From food to factory]]></article-title>
<source><![CDATA[Microbial Cell Factories]]></source>
<year>2017</year>
<volume>16</volume>
<numero>55</numero>
<issue>55</issue>
<page-range>1-15</page-range></nlm-citation>
</ref>
<ref id="B18">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Stiles]]></surname>
<given-names><![CDATA[M. E.]]></given-names>
</name>
<name>
<surname><![CDATA[Holzapfel]]></surname>
<given-names><![CDATA[W. H.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Lactic acid bacteria of foods and their current taxonomy]]></article-title>
<source><![CDATA[International Journal of Food Microbiology]]></source>
<year>1997</year>
<volume>36</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>1-29</page-range></nlm-citation>
</ref>
<ref id="B19">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Yerlikaya]]></surname>
<given-names><![CDATA[O.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Probiotic potential and biochemical and technological properties of Lactococcus lactis ssp. lactis strains isolated from raw milk and kefir grains]]></article-title>
<source><![CDATA[Journal of Dairy Science]]></source>
<year>2019</year>
<volume>102</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>124-34</page-range></nlm-citation>
</ref>
<ref id="B20">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Zhang]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Liu]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
<name>
<surname><![CDATA[Su]]></surname>
<given-names><![CDATA[Y.]]></given-names>
</name>
<name>
<surname><![CDATA[Li]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
<name>
<surname><![CDATA[Sun]]></surname>
<given-names><![CDATA[Q.]]></given-names>
</name>
<name>
<surname><![CDATA[Liang]]></surname>
<given-names><![CDATA[X.]]></given-names>
</name>
<name>
<surname><![CDATA[Lv]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Antioxidative activity of lactic acid bacteria in yogurt]]></article-title>
<source><![CDATA[African Journal of Microbiology Research]]></source>
<year>2011</year>
<volume>5</volume>
<numero>29</numero>
<issue>29</issue>
<page-range>5194-201</page-range></nlm-citation>
</ref>
</ref-list>
</back>
</article>
