<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0188-6266</journal-id>
<journal-title><![CDATA[Acta universitaria]]></journal-title>
<abbrev-journal-title><![CDATA[Acta univ]]></abbrev-journal-title>
<issn>0188-6266</issn>
<publisher>
<publisher-name><![CDATA[Universidad de Guanajuato, Dirección de Investigación y Posgrado]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0188-62662018000200053</article-id>
<article-id pub-id-type="doi">10.15174/au.2018.1769</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Efecto de tres niveles de oxígeno en la atmósfera de cultivo y la adición de un antioxidante comercial en el desarrollo de embriones bovinos producidos in vitro]]></article-title>
<article-title xml:lang="en"><![CDATA[Effect of three oxygen levels in culture atmosphere and addition of commercial antioxidant in the development of bovine embryos produced in vitro]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Delgado Tiburcio]]></surname>
<given-names><![CDATA[Guadalupe Adilia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Autónoma de Nayarit  ]]></institution>
<addr-line><![CDATA[Xalisco Nayarit]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>04</month>
<year>2018</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>04</month>
<year>2018</year>
</pub-date>
<volume>28</volume>
<numero>2</numero>
<fpage>53</fpage>
<lpage>57</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0188-62662018000200053&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S0188-62662018000200053&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S0188-62662018000200053&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN El objetivo del presente estudio fue evaluar el efecto de tres atmósferas de incubación y el empleo de un antioxidante comercial en medios de cultivo semidefinidos de un sistema secuencial, sobre la producción in vitro de embriones bovinos. Después de la fertilización in vitro, los presuntos cigotos fueron asignados aleatoriamente a seis tratamientos en arreglo factorial: con y sin el complejo antioxidante (Sigma antioxidant supplement®) y tres concentraciones de oxígeno (2%, 5% y 20%) en la atmósfera de cultivo. Los resultados se analizaron mediante un Análisis de Varianza (Anova) factorial y se compararon por la prueba de Tukey (p &lt; 0.05). La atmósfera reducida en oxígeno (2%) influyó positivamente en las variablesporcentaje de blastocistos, porcentaje de mórula y número de células (p &lt; 0.05). La interacción de la atmósfera con el antioxidante resultó significativa para la variable número de células (p &lt; 0.05). El presente trabajo concluye efectos positivos con el uso de 2% de O2.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT The aim of the present study was to evaluate the effect of three oxygen concentrations in the incubation environment and the use of a commercial antioxidant in semi-defined, sequential culture media on the in vitro production of bovine embryos. After in vitro fertilization, the presumptive zygotes were randomly assigned to six treatments under factorial arrangement: with and without the antioxidant compound (Sigma antioxidant supplement®), under three oxygen concentrations (2%, 5% and 20%) in the incubation atmosphere. The results were analyzed by Análisis de Varianza (Anova), using Tukey test (p &lt; 0.05) to compare treatments. Reduced oxygen (2%), in the incubation environment, had a positive effect on blastocysts and morula production percentage, and cell number (p &lt; 0.05). It is concluded that there are positive effects in the use of reduced oxygen (2%).]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Producción de embriones bovinos in vitro]]></kwd>
<kwd lng="es"><![CDATA[antioxidante]]></kwd>
<kwd lng="es"><![CDATA[ROS]]></kwd>
<kwd lng="en"><![CDATA[in vitro production]]></kwd>
<kwd lng="en"><![CDATA[bovine embryos]]></kwd>
<kwd lng="en"><![CDATA[antioxidant]]></kwd>
<kwd lng="en"><![CDATA[ROS]]></kwd>
</kwd-group>
</article-meta>
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