<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0187-7380</journal-id>
<journal-title><![CDATA[Revista fitotecnia mexicana]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. fitotec. mex]]></abbrev-journal-title>
<issn>0187-7380</issn>
<publisher>
<publisher-name><![CDATA[Sociedad Mexicana de Fitogenética A.C.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0187-73802016000400359</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Regeneración de agave mezcalero (Agave angustifolia HAW.) a partir de embriones somáticos encapsulados]]></article-title>
<article-title xml:lang="en"><![CDATA[Regeneration of agave (Agave angustifolia HAW.) from encapsulated somatic embryos]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Arzate-Fernández]]></surname>
<given-names><![CDATA[Amaury M.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Piña-Escutia]]></surname>
<given-names><![CDATA[José L.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Norman-Mondragón]]></surname>
<given-names><![CDATA[Tomás H.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Reyes-Díaz]]></surname>
<given-names><![CDATA[Jesús I.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Guevara-Suárez]]></surname>
<given-names><![CDATA[Karen L.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Vázquez-García]]></surname>
<given-names><![CDATA[Luis M.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Autónoma del Estado de México Campus "El Cerrillo" Facultad de Ciencias Agrícolas]]></institution>
<addr-line><![CDATA[Toluca Estado de México]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad Autónoma del Estado de México Centro Universitario Tenancingo ]]></institution>
<addr-line><![CDATA[ Estado de México]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2016</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2016</year>
</pub-date>
<volume>39</volume>
<numero>4</numero>
<fpage>359</fpage>
<lpage>366</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0187-73802016000400359&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S0187-73802016000400359&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S0187-73802016000400359&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen: Como una estrategia de propagación y conservación, se obtuvieron embriones somáticos (ES) de Agave angustifolia Haw. a partir de ejes embrionarios cigóticos (EEC), cultivados en el medio de Murashige y Skoog (MS) a 25 % de su concentración, suplementado con vitaminas L2, 13.57 µmol de ácido 2,4-diclorofenoxiacético (2,4-D), 4.4 µmol de 6-bencilaminopurina (BAP) y 60 g L-1 de sacarosa. Para producir semillas sintéticas (SS), cada ES fue encapsulado en una matriz de alginato de sodio en complejo con cloruro de calcio. Se evaluaron tres concentraciones (3, 4 y 5 %) de la matriz de alginato de sodio (MAS) durante 15 y 30 min como tiempo de inmersión (TI) en cloruro de calcio, así como la concentración de las sales del medio MS (50 y 100 %) como endospermo sintético, enriquecido con 6-bencilaminopurina (BAP) (0.0, 4.4 y 8.8 µmol). También se evaluó el efecto de las tres concentraciones de la MAS en el porcentaje de germinación y de sobrevivencia de las SS. La germinación de las SS y sobrevivencia de las plántulas, 100 % en ambos casos, se observó en los ES encapsulados con 3 % de la MAS disuelta en medio MS a 100 % y suplementado con 4.4 µmol de BAP, con 15 o 30 min de TI en cloruro de calcio.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract: As a strategy for propagation and conservation, somatic embryos (SE) of Agave angustifolia Haw. were obtained from zygotic embryonic axis (EEC), using the Murashige and Skoog (MS) medium to 25 % of its concentration, supplemented with L2 vitamins, 13.57 µmol of 2,4-dichlorophenoxyacetic acid (2,4-D), 4.4 µmol of 6-benzylaminopurine (BAP) and 60 gL-1 sucrose. Each SE was encapsulated in a matrix of sodium alginate in a complex of calcium dichloride to produce synthetic seeds (SS). To determine the effect on germination percentage and immersion time (IT), three concentrations of sodium alginate (3, 4 and 5 %) immersed in time intervals of 15 and 30 min in calcium dichloride, and two concentrations of MS medium salts (50 and 100 %) supplemented with 6-benzylaminopurine (BAP) (0.0, 4.4 and 8.8 µmol), were tested. The consistency of the alginate matrix on germination percentage and SS survival were also evaluated. Full germination and plantlet survival were observed when synthetic seeds were encapsulated with 3 % sodium alginate in complex with calcium dichloride, supplemented with 4.4 µmol BAP at 15 or 30 min as IT in calcium dichloride.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Agave angustifolia]]></kwd>
<kwd lng="es"><![CDATA[embriogénesis somática]]></kwd>
<kwd lng="es"><![CDATA[encapsulación]]></kwd>
<kwd lng="es"><![CDATA[semilla sintética]]></kwd>
<kwd lng="en"><![CDATA[Agave angustifolia]]></kwd>
<kwd lng="en"><![CDATA[somatic embryogenesis]]></kwd>
<kwd lng="en"><![CDATA[encapsulation]]></kwd>
<kwd lng="en"><![CDATA[synthetic seed]]></kwd>
</kwd-group>
</article-meta>
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<article-title xml:lang=""><![CDATA[In vitro multiplication of Agave cocui Trelease through axillary buds]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Salazar]]></surname>
<given-names><![CDATA[E.]]></given-names>
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<name>
<surname><![CDATA[González]]></surname>
<given-names><![CDATA[P.]]></given-names>
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<name>
<surname><![CDATA[Hernández]]></surname>
<given-names><![CDATA[C.]]></given-names>
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</person-group>
<source><![CDATA[Agronomía Tropical]]></source>
<year>2009</year>
<volume>59</volume>
<page-range>129-35</page-range></nlm-citation>
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