<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0185-3309</journal-id>
<journal-title><![CDATA[Revista mexicana de fitopatología]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. mex. fitopatol]]></abbrev-journal-title>
<issn>0185-3309</issn>
<publisher>
<publisher-name><![CDATA[Sociedad Mexicana de Fitopatología A.C.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0185-33092020000300105</article-id>
<article-id pub-id-type="doi">10.18781/r.mex.fit.2004-2</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Bacterias simbióticas asociadas a Prosthechea citrina, una orquídea endémica de México]]></article-title>
<article-title xml:lang="en"><![CDATA[Symbiotic bacteria associated to Prosthechea citrina, a Mexican endemic orchid]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Santiago-Gerónimo]]></surname>
<given-names><![CDATA[Tomasita]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Lozoya-Saldaña]]></surname>
<given-names><![CDATA[Héctor]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rodríguez-Mejía]]></surname>
<given-names><![CDATA[María Lourdes]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Autónoma Chapingo  ]]></institution>
<addr-line><![CDATA[Chapingo Estado de México]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad Autónoma Chapingo  ]]></institution>
<addr-line><![CDATA[Chapingo Estado de México]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2020</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2020</year>
</pub-date>
<volume>38</volume>
<numero>3</numero>
<fpage>398</fpage>
<lpage>407</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0185-33092020000300105&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S0185-33092020000300105&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S0185-33092020000300105&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen. En el proceso de cultivo in vitro de tejidos de plantas, se presentan microorganismos potencialmente fitopatógenos y/o aparentemente contaminantes, que eventualmente ocasionan perdidas de material vegetal. La presente investigación tuvo como objetivo identificar a bacterias endófitas asociadas a Prosthechea citrina, una orquídea endémica de México, mediante el aislamiento, cultivo in vitro y secuenciación del gen 16S del ARN ribosomal de las cepas bacterianas. Se identificaron a Aeromonas hydrophila y Enterobacter sp. como simbiontes, no patogénicos, del bulbo y de base de hoja y a A. hydrophila en la parte media y el ápice foliar. Se evaluaron cuatro antibióticos para su control in vitro mediante el método de disco-placa, cuantificando el diámetro de crecimiento de la colonia. El mayor halo de inhibición, con significancia estadística, se obtuvo con oxitetraciclina para A. hydrophila y con ampicilina para Enterobacter sp. Se demuestra la presencia de bacterias endófitas, con especificidad de ubicación en el tejido, así como el antibiótico correspondiente que las inhibe.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract. Along the process of in vitro plant tissue culture, potential plant pathogenic and/or apparent contaminant microorganisms occur, which eventually cause loss of plant material. The objective of this research was to identify endophytic bacteria in Prosthechea citrina, an endemic Mexican orchid, by isolating, in vitro culturing, and sequencing the 16S gen from ribosomal RNA of the bacterial isolates. Aeromonas hydrophila and Enterobacter sp. were identified as symbionts, non-pathogenic, from the bulb and the lower part of the leaf, and A. hydrophila in the middle and top of the leaf. Four antibiotics were evaluated for their in vitro control using the disc-plate method, quantifying the colony diameter growth. The highest statistically significant inhibition halos were obtained with Oxytetracycline for A. hydrophila, and with ampicillin for Enterobacter sp. The presence of endophytic bacteria is demonstrated, with tissue specificity location, as well as the corresponding inhibitory antibiotic.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Simbiosis]]></kwd>
<kwd lng="es"><![CDATA[bacterias endófitas]]></kwd>
<kwd lng="es"><![CDATA[antibióticos]]></kwd>
<kwd lng="en"><![CDATA[Symbioses]]></kwd>
<kwd lng="en"><![CDATA[endophytic bacteria]]></kwd>
<kwd lng="en"><![CDATA[antibiotics]]></kwd>
</kwd-group>
</article-meta>
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