<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0185-3309</journal-id>
<journal-title><![CDATA[Revista mexicana de fitopatología]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. mex. fitopatol]]></abbrev-journal-title>
<issn>0185-3309</issn>
<publisher>
<publisher-name><![CDATA[Sociedad Mexicana de Fitopatología A.C.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0185-33092019000200010</article-id>
<article-id pub-id-type="doi">10.18781/r.mex.fit.1902-6</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Identificación de Tomato brown rugose fruit virus por RT-PCR de una región codificante de la replicasa (RdRP)]]></article-title>
<article-title xml:lang="en"><![CDATA[Identification of Tomato brown rugose fruit virus by RT-PCR from a coding region of replicase (RdRP)]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rodríguez-Mendoza]]></surname>
<given-names><![CDATA[Johan]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[García-Ávila]]></surname>
<given-names><![CDATA[Clemente de Jesús]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[López-Buenfil]]></surname>
<given-names><![CDATA[José Abel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Araujo-Ruiz]]></surname>
<given-names><![CDATA[Karina]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Quezada-Salinas]]></surname>
<given-names><![CDATA[Andrés]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Cambrón-Crisantos]]></surname>
<given-names><![CDATA[José Manuel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ochoa-Martínez]]></surname>
<given-names><![CDATA[Daniel Leobardo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Centro Nacional de Referencia Fitosanitaria Dirección General de Sanidad Vegetal ]]></institution>
<addr-line><![CDATA[Tecámac Estado de México]]></addr-line>
<country>México</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Colegio de Postgraduados Postgrado en Fitosanidad-Fitopatología ]]></institution>
<addr-line><![CDATA[Texcoco Estado de México]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2019</year>
</pub-date>
<volume>37</volume>
<numero>2</numero>
<fpage>345</fpage>
<lpage>356</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0185-33092019000200010&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S0185-33092019000200010&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S0185-33092019000200010&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen. En la actualidad es de importancia estratégica contar con herramientas de diagnóstico rápido y preciso que permitan conocer si el material de interés está infectado con algún virus de importancia cuarentenaria o económica. Por tal motivo, en el presente trabajo se diseñó un par de oligonucleótidos específicos para la detección del ToBRFV por RT-PCR, considerando una región de la secuencia codificante de la RdRP localizada en el ORF1 y la metodología para detectarlo fue estandarizada. Adicionalmente, los amplicones fueron clonados y secuenciados, los productos fueron usados para predecir la relación evolutiva entre ToMMV, ToMV, TMV y ToBRFV por el método Maximum Likelihood. Los resultados indicaron que los oligonucleótidos diseñados en el presenté trabajo permiten la identificación de manera rápida y específica del ToBRFV.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract. Currently, it is of strategic importance to have fast and accurate diagnostic tools that allow us to know if the material of interest is infected with a quarantine or economic important virus. For this reason in the present work we designed a pair of specific oligonucleotides for the detection of ToBRFV by RT-PCR, considering a region of the coding sequence of the RdRP located in the ORF1 and the methodology to detect it was standardized. Additionally, the amplicons were cloned and sequenced, the products were used to predict the evolutionary relationship between ToMMV, ToMV, TMV and ToBRFV by the Maximum Likelihood method. The results indicated that the oligonucleotides designed in the present work allows the identification of fast and specific ToBRFV.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Solanaceae]]></kwd>
<kwd lng="es"><![CDATA[Tobamovirus]]></kwd>
<kwd lng="es"><![CDATA[oligonucleótidos específicos]]></kwd>
<kwd lng="es"><![CDATA[estandarización]]></kwd>
<kwd lng="en"><![CDATA[Solanaceae]]></kwd>
<kwd lng="en"><![CDATA[Tobamovirus]]></kwd>
<kwd lng="en"><![CDATA[specific oligonucleotides]]></kwd>
<kwd lng="en"><![CDATA[standardization]]></kwd>
</kwd-group>
</article-meta>
</front><back>
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