<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0185-3309</journal-id>
<journal-title><![CDATA[Revista mexicana de fitopatología]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. mex. fitopatol]]></abbrev-journal-title>
<issn>0185-3309</issn>
<publisher>
<publisher-name><![CDATA[Sociedad Mexicana de Fitopatología A.C.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0185-33092019000200005</article-id>
<article-id pub-id-type="doi">10.18781/r.mex.fit.1902-2</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Identificación de begomovirus en tomate y determinación de su transmisión por semilla en Atlacomulco, Estado de México]]></article-title>
<article-title xml:lang="en"><![CDATA[Identification of begomoviruses in tomato and determination of seed transmission in Atlacomulco, Mexico State]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Zuñiga Romano]]></surname>
<given-names><![CDATA[Maria del Carmen]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ochoa Martínez]]></surname>
<given-names><![CDATA[Daniel Leobardo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rojas Martínez]]></surname>
<given-names><![CDATA[Reyna Isabel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Zamora Macorra]]></surname>
<given-names><![CDATA[Erika Janet]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Colegio de Postgraduados Posgrado en Fitosanidad-Fitopatología ]]></institution>
<addr-line><![CDATA[Texcoco Estado de México]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad Autónoma Chapingo  ]]></institution>
<addr-line><![CDATA[Texcoco Estado de México]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2019</year>
</pub-date>
<volume>37</volume>
<numero>2</numero>
<fpage>279</fpage>
<lpage>286</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0185-33092019000200005&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S0185-33092019000200005&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S0185-33092019000200005&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen. En un invernadero de alta tecnología ubicado en Atlacomulco, Estado de México, se han observado incidencias del 60 al 90% de plantas con síntomas típicos de begomovirus. Los objetivos del presente estudio fueron identificar begomovirus presentes, conocer si se transmiten por semilla, determinar la especie de mosca blanca y si ésta es portadora de los virus. Hojas de plantas adultas con síntomas se analizaron con oligonucleótidos universales para begomovirus. Semillas de frutos de plantas positivas a begomovirus se sembraron en almácigos para obtener plántulas, éstas y parte de las semillas se analizaron por PCR para detectar begomovirus. Adultos de mosca blanca se analizaron con oligonucleótidos específicos del gen mitocondrial de la subunidad I del citocromo C oxidasa y con oligonucleótidos universales para begomovirus. Las secuencias de los productos obtenidos por PCR de plantas con síntomas tuvieron una similitud de 99% con el Tomato severe leaf curl virus (ToSLCV). No se detectaron begomovirus en semilla y plántula. Las secuencias obtenidas por PCR de mosca blanca en el invernadero tuvieron 99% de similitud con Bemisia tabaci y los individuos analizados fueron portadores del ToSLCV. Este es el primer reporte del ToSLCV asociado a tomate en invernadero en México.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract. In a high-tech greenhouse located at Atlacomulco, Mexico State, incidences from 60 to 90% of plants showing symptoms associated to begomoviruses have been observed. The objectives of this study were to identify the begomoviruses present, to know if they are transmitted by seed, to determine the species of whitefly and if they are carriers of the virus. Leaves of adult symptomatic plants were analyzed with universal primers for begomoviruses. Seeds of fruits from positive plants were seeded to obtain seedlings; seeds and seedlings were analyzed by PCR for begomoviruses. Adults whiteflies were analyzed with specific primers to the mitochondrial gene of the cytochrome C oxidase subunit I and universal primers for begomoviruses. Sequences of PCR products from symptomatic plants had 99% of identity with Tomato severe leaf curl virus (ToSLCV). No begomoviruses were detected in seed and seedlings. Sequences from the whiteflies present in the greenhouse had an identity of 99% with Bemisia tabaci and they were carriers of ToSLCV. This is the first report of ToSLCV infecting tomato grown in greenhouse in Mexico.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Tomato severe leaf curl virus]]></kwd>
<kwd lng="es"><![CDATA[Bemisia tabaci]]></kwd>
<kwd lng="es"><![CDATA[Solanum lycopersicum]]></kwd>
<kwd lng="en"><![CDATA[Tomato severe leaf curl virus]]></kwd>
<kwd lng="en"><![CDATA[Bemisia tabaci]]></kwd>
<kwd lng="en"><![CDATA[Solanum lycopersicum]]></kwd>
</kwd-group>
</article-meta>
</front><back>
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