<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0185-3309</journal-id>
<journal-title><![CDATA[Revista mexicana de fitopatología]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. mex. fitopatol]]></abbrev-journal-title>
<issn>0185-3309</issn>
<publisher>
<publisher-name><![CDATA[Sociedad Mexicana de Fitopatología A.C.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0185-33092019000100012</article-id>
<article-id pub-id-type="doi">10.18781/r.mex.fit.1810-5</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Primer reporte de Tomato brown rugose fruit virus (ToBRFV) en Michoacán, México]]></article-title>
<article-title xml:lang="en"><![CDATA[First report of Tomato brown rugose fruit virus (ToBRFV) in Michoacan, Mexico]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Cambrón-Crisantos]]></surname>
<given-names><![CDATA[José Manuel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rodríguez-Mendoza]]></surname>
<given-names><![CDATA[Johan]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Valencia-Luna]]></surname>
<given-names><![CDATA[Jessica Berenice]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Alcasio Rangel]]></surname>
<given-names><![CDATA[Salomé]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[García-Ávila]]></surname>
<given-names><![CDATA[Clemente de Jesús]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[López-Buenfil]]></surname>
<given-names><![CDATA[José Abel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ochoa-Martínez]]></surname>
<given-names><![CDATA[Daniel Leobardo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Dirección General de Sanidad Vegetal Centro Nacional de Referencia Fitosanitaria ]]></institution>
<addr-line><![CDATA[ Estado de México]]></addr-line>
<country>México</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Colegio de Postgraduados Postgrado en Fitosanidad-Fitopatología ]]></institution>
<addr-line><![CDATA[Texcoco Estado de México]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2019</year>
</pub-date>
<volume>37</volume>
<numero>1</numero>
<fpage>185</fpage>
<lpage>192</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0185-33092019000100012&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S0185-33092019000100012&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S0185-33092019000100012&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen A nivel mundial, la producción de jitomate y chile es de gran relevancia. Recientemente, en Israel y Jordania se notificó la presencia de un nuevo miembro del género Tobamovirus en plantaciones de jitomate, al cual se le llamó Tomato brown rugose fruit virus (ToBRFV). Los frutos de plantas infectadas manifiestan áreas amarillas, necróticas o de color marrón incluso rugosidades. En los municipios de Yurécuaro y Tanhuato, Michoacán, se han observado cultivos de jitomate y chile con síntomas similares a los antes mencionados por lo que el objetivo del presente trabajo fue determinar si el ToBRFV se encuentra presente en estas localidades. Se colectó tejido foliar de jitomate con síntomas y se realizó RT-PCR con iniciadores que amplifican un segmento del ORF2 del genoma de este virus; además, se prepararon rejillas para su observación al microscopio electrónico de transmisión (MET). Se obtuvo el amplicón esperado y las secuencias tuvieron una similitud de 99 a 100 % con ToBRFV. Al MET se observaron partículas virales en forma de varilla rígida típicas de tobamovirus. Hasta donde sabemos este es el primer reporte de la presencia del ToBRFV asociado a plantas de jitomate y chile cultivados en México.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract The production of tomato and pepper is of great importance worldwide. Recently, in Israel and Jordan, the presence of a new member of the Tobamovirus genus was reported in tomato crops, which was called Tomato brown rugose fruit virus (ToBRFV). The fruits of infected plants can develop yellow areas, roughness, as well as necrotic or brown areas. In Yurecuaro and Tanhuato municipalities of Michoacan, crops of tomato and pepper have shown symptoms similar to those described, thus the objective was to determine if the ToBRFV is present in those places. Leaf tissue of tomato with symptoms was included and RT-PCR was performed with primers that amplify a segment of the ORF2 of the genome of this virus; further, grids were prepared for observation under the transmission electron microscope (TEM). The expected amplicon was obtained and the sequences had a similarity of 99 to 100 % with ToBRFV. At the TEM, rigid rod-shaped viral particles typical of tobamoviruses were observed. To our knowledge, it is the first report of the presence of ToBRFV associated to tomato and pepper plants grown in Mexico.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Tobamovirus]]></kwd>
<kwd lng="es"><![CDATA[Solanaceae]]></kwd>
<kwd lng="es"><![CDATA[RT-PCR]]></kwd>
<kwd lng="es"><![CDATA[microscopía electrónica de transmisión]]></kwd>
<kwd lng="en"><![CDATA[Tobamovirus]]></kwd>
<kwd lng="en"><![CDATA[Solanaceae]]></kwd>
<kwd lng="en"><![CDATA[RT-PCR]]></kwd>
<kwd lng="en"><![CDATA[transmission electron microscopy]]></kwd>
</kwd-group>
</article-meta>
</front><back>
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</article>
