<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0185-3309</journal-id>
<journal-title><![CDATA[Revista mexicana de fitopatología]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. mex. fitopatol]]></abbrev-journal-title>
<issn>0185-3309</issn>
<publisher>
<publisher-name><![CDATA[Sociedad Mexicana de Fitopatología A.C.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0185-33092017000200326</article-id>
<article-id pub-id-type="doi">10.18781/r.mex.fit.1701-2</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Etiología de la mancha necrótica del Agave angustifolia: Una aproximación in vitro hacía su control biológico]]></article-title>
<article-title xml:lang="en"><![CDATA[Etiology of a black spot symptom in Agave angustifolia: An in vitro approach towards its biological control]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Duque-Bautista]]></surname>
<given-names><![CDATA[Horacio]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Zurita-Vásquez]]></surname>
<given-names><![CDATA[Guilibaldo Gabriel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Pacheco-Hernández]]></surname>
<given-names><![CDATA[Yesenia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Villa-Ruano]]></surname>
<given-names><![CDATA[Nemesio]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad de la Sierra Sur  ]]></institution>
<addr-line><![CDATA[Miahuatlán de Porfirio Díaz Oaxaca]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,IPN Centro de Investigación en Biotecnología Aplicada ]]></institution>
<addr-line><![CDATA[ Tlaxcala]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Universidad de la Sierra Sur  ]]></institution>
<addr-line><![CDATA[Miahuatlán de Porfirio Díaz Oaxaca]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2017</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2017</year>
</pub-date>
<volume>35</volume>
<numero>2</numero>
<fpage>326</fpage>
<lpage>337</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0185-33092017000200326&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S0185-33092017000200326&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S0185-33092017000200326&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen. Presentamos la identificación molecular de una especie de Cladosporium aislada de las manchas necróticas de los tallos enfermos de Agave angustifolia, los cuales se colectaron en San Luis Amatlán Oaxaca, México. Los análisis de los espaciadores transcritos internos (ITS) del gen de RNA ribosomal 18S y de la secuencia parcial codificante (cDNA) de una proteína alergénica conservada de choque térmico 70 (HSP70), revelaron su similitud con Cladosporium herbarum (Pers.: Fr.) Link. La evaluación in vitro de los extractos etanólicos de las raíces Zaluzania montagnifolia y de sus principales ent-kaurenoides resultó en la inhibición del crecimiento normal del hongo. La concentración mínima inhibitoria (MIC) se obtuvo por el método de dilución en agar y microdilución en caldo. El extracto etanólico, el ácido ent-kaurenoico (ent-16kauren-19-oic acid) y el ácido grandiflorenico (entkaur-9(11),16-dien-19-oic acid) mostraron valores MIC de 150.4±3.5, 100.3±2.1 y 80.7±1.4 &#956;g mL-1, respectivamente.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract. We present the molecular identification of a Cladosporium species isolated from necrotic spots of diseased stalks of Agave angustifolia, which were collected in San Luis Amatlán, Oaxaca, Mexico. Analyses of the internal transcribed spacer (ITS) from the 18S ribosomal RNA gene and the partial coding region (cDNA) from an allergenic conserved heat shock protein 70 (HSP70), revealed its similarity with Cladosporium herbarum (Pers.: Fr.) Link. The in vitro assessment of the ethanolic extracts from Zaluzania montagnifolia roots and its main ent-kaurenoids resulted in the growth inhibition of the fungus. Minimum inhibitory concentration (MIC) values were obtained by the agar dilution and broth microdilution methods. The ethanolic extract, ent-kaurenoic acid (ent16-kauren-19-oic acid) and grandiflorenic acid (ent-kaur-9(11),16-dien-19-oic acid) showed MIC values of 150.4±3.5, 100.3±2.1, and 80.7±1.4 &#956;g mL-1, respectively.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Cladosporium herbarum]]></kwd>
<kwd lng="es"><![CDATA[ITS]]></kwd>
<kwd lng="es"><![CDATA[maguey mezcalero]]></kwd>
<kwd lng="es"><![CDATA[derivados del ent-kaureno]]></kwd>
<kwd lng="es"><![CDATA[inhibición del crecimiento]]></kwd>
<kwd lng="en"><![CDATA[Cladosporium herbarum]]></kwd>
<kwd lng="en"><![CDATA[ITS]]></kwd>
<kwd lng="en"><![CDATA[maguey mezcalero]]></kwd>
<kwd lng="en"><![CDATA[ent-kaurene derivatives]]></kwd>
<kwd lng="en"><![CDATA[growth inhibition]]></kwd>
</kwd-group>
</article-meta>
</front><back>
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