<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0065-1737</journal-id>
<journal-title><![CDATA[Acta zoológica mexicana]]></journal-title>
<abbrev-journal-title><![CDATA[Acta Zool. Mex]]></abbrev-journal-title>
<issn>0065-1737</issn>
<publisher>
<publisher-name><![CDATA[Instituto de Ecología A.C.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0065-17372017000100018</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Estandarización y validación de la prueba de PCR anidada para el diagnóstico de especies del género Xyleborus (Coleoptera: Curculionidae: Scolytinae)]]></article-title>
<article-title xml:lang="en"><![CDATA[Standardization and validation of the nested PCR test for the diagnosis of the genus Xyleborus species (Coleoptera: Curculionidae: scolytinae)]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Sosa-Castillo]]></surname>
<given-names><![CDATA[María Elena]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Lara Reyna]]></surname>
<given-names><![CDATA[Joel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ortega Arenas]]></surname>
<given-names><![CDATA[Laura Delia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Hernández]]></surname>
<given-names><![CDATA[Alfonsina Judith]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Colegio de Postgraduados  ]]></institution>
<addr-line><![CDATA[Montecillo, Texcoco Edo. de México]]></addr-line>
<country>México</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Colegio de Postgraduados  ]]></institution>
<addr-line><![CDATA[Champotón Campeche]]></addr-line>
<country>México</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Colegio de Postgraduados  ]]></institution>
<addr-line><![CDATA[Montecillo, Texcoco Edo. de México]]></addr-line>
<country>México</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>04</month>
<year>2017</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>04</month>
<year>2017</year>
</pub-date>
<volume>33</volume>
<numero>1</numero>
<fpage>18</fpage>
<lpage>26</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0065-17372017000100018&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S0065-17372017000100018&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S0065-17372017000100018&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen: En este estudio se estandarizó y validó la técnica de PCR anidada para la detección rápida, sensible y confiable de especies del género Xyleborus mediante el uso de los &#8220;primers&#8221; externos CI-J-2183 y TL2-N-3014 e internos J2210 y N2739, que amplifican una banda de 500 pb de la región del gen mitocondrial Citocromo Oxidasa subunidad 1(CO1). Asimismo, se realizó la extracción de ADN de 26 ejemplares de Xyleborus con el kit Qiagen DNeasy® mericom Food (DMF), no reportado previamente su uso para su aplicación en insectos, que resultó en ADN suficiente y de alta calidad para reacciones de amplificación por PCR. El método permitió procesar un solo insecto por extracción, y obtener material genético de muestras conservadas en alcohol de hasta ocho años de antigüedad. El límite de detección se definió hasta una concentración de 780 pg/&#956;l. Se optimizó la PCR en un volumen final de 15 µL sin comprometer calidad de la amplificación. La técnica estandarizada permitió la obtención de ADN de calidad, lo que aseguró alta reproducibilidad y sensibilidad en la detección de especies de Xyleborus y la secuenciación parcial del gen CO1 para las siete especies estudiadas; las secuencias consenso fueron analizadas por homología y depositadas en el GenBank.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract: In this study it was standardized and validated the nested PCR test for rapid, sensitive and reliable detection of species of the genus Xyleborus using external &#8220;primers&#8221; CI-J-2183 and TL2-N-3014 and internal primers J2210 and N2739 that amplify a band of 500 bp in the region of the gene mitochondrial Cytochrome Oxidase subunit 1(CO1). Also, DNA extraction from 26 specimens of Xyleborus was realized with kit Qiagen DNeasy ® mericom Food (DMF), not previously reported its use for their application in insects, which resulted in enough DNA and high-quality for amplification by PCR reactions. The method allowed to process a single insect by extraction, and obtain genetic material from specimens preserved in alcohol of up to eight years old. The detection limit was defined up to a concentration of 780 pg/&#956;L. The PCR was optimized in a final volume of 15 µL without compromising quality of amplification. The standardized test allowed quality DNA, which ensured high reproducibility and sensitivity in the detection of species of Xyleborus and partial sequencing of the CO1 gene to the seven species studied; consensus sequences were analyzed by homology and deposited in the GenBank.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Escarabajos ambrosiales]]></kwd>
<kwd lng="es"><![CDATA[identificación molecular]]></kwd>
<kwd lng="es"><![CDATA[diagnóstico]]></kwd>
<kwd lng="es"><![CDATA[gen CO1]]></kwd>
<kwd lng="en"><![CDATA[Ambrosial beetles]]></kwd>
<kwd lng="en"><![CDATA[molecular identification]]></kwd>
<kwd lng="en"><![CDATA[diagnosis]]></kwd>
<kwd lng="en"><![CDATA[CO1 gene]]></kwd>
</kwd-group>
</article-meta>
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