Study of bone cells by quantitative phase microscopy using a Mirau interferometer

J. González-Lapreaª *, A. Márquez^b, K. Noris-Suárez^b, and R. Escalonaª

ª Laboratorio de Óptica e Interferometría, Departamento de Física. * Laboratorio de Óptica e Interferometría (006), Edificio de Física y Electrónica I, Universidad Simón Bolívar, Carretera Baruta - Hoyo de la Puerta, valle de Sartenejas, Caracas, 1080-A, Venezuela, Tel: +58 212 906 3522; Fax: +58 212 906 3600 e-mail: jeglaprea@usb.ve

^b Laboratorio de Bioingeniería de tejidos Departamento de Biología Celular, Universidad Simón Bolívar (Venezuela).

Abstract

The article presents the use of an interference microscope, using a Mirau objective for the study of the early adhesion process of osteoblast-like bone cells, using the phase shifting technique. The process is carried out on surgical stainless steel surfaces of interest for the development of bone implants. Experimental phase maps are directly related to cell profiles. These phase maps are obtained for several adhesion times, which indicate morphological changes in cells. Mainly the change in height profiles through time and the interaction with other surrounding cells are observed. The experimental system used is appropriate for the time scales observed, in the order of hours, showing strength and precision in the calculation of the optical phase.

Keywords: Interferential microscopy; optical phase maps; osteoblast-like cells; quantitative phase microscopy; opaque surfaces.

Resumen

El artículo presenta el uso de un microscopio interferencial, empleando un objetivo de Mirau, para el estudio del proceso de adhesión temprana de células óseas tipo osteoblasto, por medio de la técnica de desplazamiento de fase. El proceso es llevado a cabo sobre superficies de acero quirúrgico, que son de interés en el desarrollo de prótesis óseas. Los mapas de fase óptica obtenidos a diversos tiempos de adhesión muestran cambios morfológicos en las células; principalmente se observa el cambio en la altura de los perfiles de la fase a través del tiempo y la formación de conexiones intercelulares. El sistema de medición empleado es apropiado para las escalas de tiempo de evolución exhibidas por el sistema celular (del orden de horas), presentando además alta robustez y precisión en el cálculo de la fase óptica.

Descriptores: Microscopía interferencial; mapas de fase óptica; células osteoblasto; microscopía de fase cuantitativa; superficies opacas.

PACS: 42.87.Bg; 87.17.Ee

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Acknowledgments

This work was supported by FONACIT-Venezuela, Research Project Number G-1997000593, by FONACIT-Venezuela, Mision Ciencia Fellowship and Simon Bolívar University, Research Group Project USB DID-GID61. Tanks to the Materials Lab from the Venezuelan institute for the scientific research by the steel discs provided.

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