<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0028-3746</journal-id>
<journal-title><![CDATA[Neumología y cirugía de tórax]]></journal-title>
<abbrev-journal-title><![CDATA[Neumol. cir. torax]]></abbrev-journal-title>
<issn>0028-3746</issn>
<publisher>
<publisher-name><![CDATA[Sociedad Mexicana de Neumología y Cirugía de Tórax; Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas; Sociedad Cubana de Neumología; Sociedad Paraguaya de Neumología; Sociedad Boliviana de Neumología.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0028-37462019000300290</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Identificación rápida de micobacterias no tuberculosas en Cuba por las técnicas «GenoType Mycobacterium CM/AS»]]></article-title>
<article-title xml:lang="en"><![CDATA[Rapid identification of nontuberculous mycobacteria in Cuba by the «GenoType Mycobacterium CM/AS» techniques]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Sardiñas-Aragón]]></surname>
<given-names><![CDATA[Misleidis]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Mederos-Cuervo]]></surname>
<given-names><![CDATA[Lilian María]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[García-León]]></surname>
<given-names><![CDATA[Grechen]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rosarys-Martínez]]></surname>
<given-names><![CDATA[María]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Lemus-Molina]]></surname>
<given-names><![CDATA[Dihadenys]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Díaz-Rodríguez]]></surname>
<given-names><![CDATA[Raúl]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Instituto de Medicina Tropical Pedro Kourí Laboratorio Nacional de Referencia e Investigaciones de Tuberculosis, Lepra y Micobacterias ]]></institution>
<addr-line><![CDATA[La Habana ]]></addr-line>
<country>Cuba</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>09</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>09</month>
<year>2019</year>
</pub-date>
<volume>78</volume>
<numero>3</numero>
<fpage>290</fpage>
<lpage>295</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0028-37462019000300290&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S0028-37462019000300290&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S0028-37462019000300290&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen:  Introducción:  En los últimos años las infecciones ocasionadas por micobacterias no tuberculosas han tenido un incremento notable en varias regiones del mundo, asociado fundamentalmente a infecciones respiratorias en individuos inmunocomprometidos. La identificación de micobacterias no tuberculosas es engorrosa y puede demorar varias semanas.  Objetivo:  Aplicar las técnicas moleculares GenoType Mycobacterium CM/AS para la identificación de micobacterias no tuberculosas en Cuba y comparar con los resultados de la prueba convencional.  Material y métodos:  Se utilizaron 73 aislados de micobacterias no tuberculosas procedentes de muestras pulmonares recibidas de unidades de salud ubicadas en varias provincias de Cuba en el período abril 2017-diciembre 2018. Para la identificación de especies se utilizaron técnicas convencionales y las técnicas moleculares GenoType CM/AS.  Resultados:  Las especies identificadas más predominantes fueron las pertenecientes al complejo Mycobacterium avium-intracellulare (46.6%) y Mycobacterium fortuitum (31.5%). Se hallaron cuatro aislados de M. simiae, especie poco frecuente en Cuba. Al comparar los resultados de ambas técnicas se obtuvo una concordancia de 91.3%.  Conclusión:  Las técnicas GenoType Mycobacterium CM/AS demostraron ser herramientas útiles en la identificación rápida de micobacterias en el Laboratorio de Referencia, brindando resultados precisos en menor tiempo, logrando acortar significativamente el tiempo diagnóstico. Las especies más frecuentes en Cuba siguen siendo el complejo Mycobacterium avium-intracellulare y Mycobacterium fortuitum.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract:  Introduction:  In recent years, infections caused by nontuberculous mycobacteria (NTM) have had a remarkable increase in several regions of the world, mainly associated with respiratory infections in immunocompromised individuals. The identification of MNT is cumbersome and may take several weeks.  Objective:  Apply the molecular techniques GenoType Mycobacterium CM/AS for the identification of MNT in Cuba, and compare with the results of conventional tests.  Material and methods:  We used 73 MNT isolates from lung samples received from health units located in several provinces of Cuba in the period April 2017 - December 2018. For the identification of species, conventional techniques and molecular techniques were used GenoType CM/AS.  Results:  The most predominant identified species were those belonging to the M. avium-intracellulare complex (46.6%) and the Mycobacterium fortuitum (31.5%). Four isolates of M. simiae, a rare species in Cuba, were found. When comparing the results of both techniques, a concordance of 91.3% was obtained.  Conclusion:  GenoType Mycobacterium CM/AS techniques proved to be useful tools in the rapid identification of mycobacteria in the Reference Laboratory, providing accurate results in less time, significantly shortening the diagnostic time. The most frequent species in Cuba continue to be the M. avium-intracellulare complex and the Mycobacterium fortuitum.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Micobacterias no tuberculosas]]></kwd>
<kwd lng="es"><![CDATA[identificación de especie]]></kwd>
<kwd lng="es"><![CDATA[GenoType Mycobacterium CM]]></kwd>
<kwd lng="es"><![CDATA[GenoType Mycobacterium AS]]></kwd>
<kwd lng="en"><![CDATA[Non tuberculous mycobacteria (NTM)]]></kwd>
<kwd lng="en"><![CDATA[species identification]]></kwd>
<kwd lng="en"><![CDATA[GenoType Mycobacterium CM]]></kwd>
<kwd lng="en"><![CDATA[GenoType Mycobacterium AS]]></kwd>
</kwd-group>
</article-meta>
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<volume>44</volume>
<numero>6</numero>
<issue>6</issue>
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</article>
