<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0188-4999</journal-id>
<journal-title><![CDATA[Revista internacional de contaminación ambiental]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. Int. Contam. Ambient]]></abbrev-journal-title>
<issn>0188-4999</issn>
<publisher>
<publisher-name><![CDATA[Universidad Nacional Autónoma de México, Instituto de Ciencias de la Atmósfera y Cambio Climático]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0188-49992023000100107</article-id>
<article-id pub-id-type="doi">10.20937/rica.54471</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Desarrollo de un método analítico para la determinación de las especies Cr(III) y Cr(VI) en medio acuoso]]></article-title>
<article-title xml:lang="en"><![CDATA[Development of an analytical method for the determination of Cr(III) and Cr(VI) species in an aqueous medium]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Andrade]]></surname>
<given-names><![CDATA[Charity]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Cáceres]]></surname>
<given-names><![CDATA[Ana]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[López]]></surname>
<given-names><![CDATA[Gabriela]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Morales]]></surname>
<given-names><![CDATA[Ever]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad del Zulia Departamento de Química Laboratorio de Química Ambiental]]></institution>
<addr-line><![CDATA[Maracaibo Zulia]]></addr-line>
<country>Venezuela</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad del Zulia Departamento de Química Laboratorio de Desarrollo de Métodos Analíticos]]></institution>
<addr-line><![CDATA[Maracaibo Zulia]]></addr-line>
<country>Venezuela</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López  ]]></institution>
<addr-line><![CDATA[ Manabí]]></addr-line>
<country>Ecuador</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2023</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2023</year>
</pub-date>
<volume>39</volume>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0188-49992023000100107&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S0188-49992023000100107&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S0188-49992023000100107&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN El cromo está presente en la naturaleza principalmente como las especies Cr(III) y Cr(VI). El Cr(III) se conoce como oligoelemento esencial, mientras que el Cr(VI) presenta toxicidad aguda. Se desarrolló un método cromatográfico (HPLC-iónico) para la determinación de ambas especies de cromo en medio acuoso contaminado durante su tratamiento por fitorremediación. Para esto se requirió la formación previa del complejo aniónico [Cr(III)-EDTA]-. Se evaluó en modo isocrático la fuerza iónica de la fase móvil y en modo gradiente la velocidad de flujo y el tiempo de gradiente. El Cr(VI) presentó una fuerte interacción con la fase estacionaria, siendo adecuada la determinación con 9.0 mM Na2CO3/3.0 mM NaHCO3, de mayor fuerza iónica. Para Cr(III) la determinación fue con 2.8 mM Na2CO3/3.4 mM NaHCO3. Mediante el uso del gradiente de concentración se logró separar y cuantificar simultáneamente ambas especies en las siguientes condiciones: fase móvil A: 2.8 mM Na2CO3/3.4 mM NaHCO3 con introducción gradual de B: 9.0 mM Na2CO3/3.0 mM NaHCO3, a t = 0.5 min, velocidad de flujo de 1.5 mL/min y volumen de muestra de 50 &#956;L. El uso de HPLC-iónico representa una alternativa eficiente, rápida y con menor impacto ambiental que las técnicas convencionales con la notable ventaja de la determinación simultánea de estas especies.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT Chromium is present in nature mainly as Cr(III) and Cr(VI) species. Cr(III) is known as an essential trace element, whereas Cr(VI) presents acute toxicity. A chromatographic method (HPLC-ionic) was developed for the determination of both chromium species in contaminated aqueous medium during their treatment by phytoremediation. For this, the previous formation of the anionic complex [Cr(III)-EDTA]- was required. The ionic strength of the mobile phase was evaluated in isocratic mode, while the flow velocity and gradient time were evaluated in gradient mode. Cr(VI) showed a strong interaction with the stationary phase and the determination with 9.0 mM Na2CO3/3.0 mM NaHCO3 of greater ionic strength was adequate, whilst for Cr(III) determination was performed with 2.8 mM Na2CO3/3.4 mM NaHCO3. The use of a concentration gradient allowed for the separation and quantification of both species simultaneously with the appropriate conditions: mobile phase A: 2.8 mM Na2CO3 2-/3.4 mM NaHCO3 with the gradual introduction of B: 9.0 mM Na2CO3/3.0 mM NaHCO3, t = 0.5 min, flow rate of 1.5 mL/min, and sample volume of 50 &#956;L. The use of HPLC-ionic represents an efficient, fast alternative with less environmental impact than conventional techniques with the remarkable advantage of the simultaneous determination of these species.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[cromatografía iónica]]></kwd>
<kwd lng="es"><![CDATA[especies de cromo]]></kwd>
<kwd lng="es"><![CDATA[gradiente]]></kwd>
<kwd lng="en"><![CDATA[ion chromatography]]></kwd>
<kwd lng="en"><![CDATA[chromium species]]></kwd>
<kwd lng="en"><![CDATA[gradient]]></kwd>
</kwd-group>
</article-meta>
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