<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0187-7380</journal-id>
<journal-title><![CDATA[Revista fitotecnia mexicana]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. fitotec. mex]]></abbrev-journal-title>
<issn>0187-7380</issn>
<publisher>
<publisher-name><![CDATA[Sociedad Mexicana de Fitogenética A.C.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0187-73802018000500551</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[EVALUACIÓN DE TRES SISTEMAS DE CULTIVO in vitro PARA LA MULTIPLICACIÓN DE MICROCORMOS DE GLADIOLO]]></article-title>
<article-title xml:lang="en"><![CDATA[EVALUATION OF THREE in vitro CULTURE SYSTEMS FOR THE MULTIPLICATION OF GLADIOLUS MICROCORMS]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Chávez-García]]></surname>
<given-names><![CDATA[J. Antonio]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Andrade-Rodríguez]]></surname>
<given-names><![CDATA[María]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Juárez-López]]></surname>
<given-names><![CDATA[Porfirio]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Villegas-Torres]]></surname>
<given-names><![CDATA[Oscar G.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Sotelo-Nava]]></surname>
<given-names><![CDATA[Héctor]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Perdomo-Roldan]]></surname>
<given-names><![CDATA[Francisco]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Autónoma del Estado de Morelos  ]]></institution>
<addr-line><![CDATA[Cuernavaca Morelos]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2018</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2018</year>
</pub-date>
<volume>41</volume>
<numero>4a</numero>
<fpage>551</fpage>
<lpage>554</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0187-73802018000500551&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S0187-73802018000500551&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S0187-73802018000500551&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN En la industria ornamental, el gladiolo (Gladiolus spp) se encuentra dentro de las principales flores de corte a nivel mundial. La propagación es a través de cormos, con tasas de multiplicación bajas, lo que hace necesario el uso de técnicas de cultivo in vitro para obtener material vegetativo con características fisiológicas idénticas, uniformes y libres de enfermedades. Sin embargo, la investigación en multiplicación in vitro de gladiolo es escasa. El sistema de inmersión temporal (RITA) se usó con éxito para producir microcormos de gladiolo in vitro. A la fecha, el sistema de biorreactores de inmersión temporal (BIT) no se ha usado para producción de microcormos de gladiolo in vitro, lo cual implica sólo 25 % del costo de un sistema RITA. El objetivo fue evaluar tres sistemas de cultivo in vitro: medio semisólido, medio líquido en inmersión parcial y medio líquido en biorreactores de inmersión temporal (BIT), para la multiplicación de microcormos de gladiolo variedad &#8220;Ámsterdam&#8221;. En el sistema BIT hubo un incremento de 91 % en la multiplicación de microcormos en comparación con el sistema semisólido y de 100 % en relación con el sistema de inmersión parcial. También el número de brotes fue mayor (41.3) en el sistema BIT que en el sistema de cultivo en medio semisólido y en el sistema de cultivo en medio líquido con inmersión parcial (5.8 y 6.5 brotes por explante). Estos resultados permitieron establecer el uso de biorreactores de inmersión temporal como mejor sistema para la multiplicación masiva de microcormos de gladiolo.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[SUMMARY In the ornamental industry, gladiolus (Gladiolus spp) is one of the main cut flowers worldwide. The propagation is through corms, with low multiplication rates, which makes it necessary to use in vitro techniques to obtain vegetative material with identical, uniform and disease-free physiological characteristics. However, the research about in vitro multiplication of gladiolus is scarce. The temporary immersion system (RITA) was used successfully to produce gladiolus microcorms in vitro. To date, the temporary immersion bioreactor system (BIT) has not been used for production of in vitro gladiolus microcorms, which represents only 25 % of the cost of a RITA system. The objective was to evaluate three in vitro culture systems: semi-solid medium, liquid medium in partial immersion and liquid medium in temporary immersion bioreactors (BIT), for the multiplication of gladiolus microcorms of the variety &#8220;Ámsterdam&#8221;. In the BIT system there was an increase of 91 % in microcorms multiplication compared to the semisolid system and 100 % in relation to the partial immersion system. The number of shoots was also higher (41.3) in the BIT system than in the semi-solid culture system and in the liquid culture system with partial immersion (5.8 and 6.5 shoots per explant). These results allowed the use of temporary immersion bioreactors to be established as the best system for the massive multiplication of gladiolus microcorms.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Gladiolus spp]]></kwd>
<kwd lng="es"><![CDATA[cultivo de tejidos vegetales]]></kwd>
<kwd lng="es"><![CDATA[Sistemas de Inmersión Temporal]]></kwd>
<kwd lng="es"><![CDATA[microcormos]]></kwd>
<kwd lng="en"><![CDATA[Gladiolus spp]]></kwd>
<kwd lng="en"><![CDATA[plant tissue culture]]></kwd>
<kwd lng="en"><![CDATA[Temporary Immersion Systems]]></kwd>
<kwd lng="en"><![CDATA[microcorms]]></kwd>
</kwd-group>
</article-meta>
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