<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0185-3309</journal-id>
<journal-title><![CDATA[Revista mexicana de fitopatología]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. mex. fitopatol]]></abbrev-journal-title>
<issn>0185-3309</issn>
<publisher>
<publisher-name><![CDATA[Sociedad Mexicana de Fitopatología A.C.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0185-33092015000100001</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Identificación de especies de Meloidogyne utilizando la secuenciación de regiones espaciadoras transcritas internas de ADN ribosomal de estadios juveniles]]></article-title>
<article-title xml:lang="en"><![CDATA[Identification of Meloidogyne species by sequencing of internal transcribed spacer regions of ribosomal DNA of juvenile stages]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Jaramillo-Pineda]]></surname>
<given-names><![CDATA[Juan]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Guerrero-Olazarán]]></surname>
<given-names><![CDATA[Martha]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Fuentes-Garibay]]></surname>
<given-names><![CDATA[José Antonio]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Viader-Salvadó]]></surname>
<given-names><![CDATA[José María]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Meza-García]]></surname>
<given-names><![CDATA[José Lorenzo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Morales-Ramos]]></surname>
<given-names><![CDATA[Lilia Hortencia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Autónoma de Nuevo León, UANL Facultad de Ciencias Biológicas Instituto de Biotecnología]]></institution>
<addr-line><![CDATA[San Nicolás de los Garza N.L.]]></addr-line>
<country>MX</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2015</year>
</pub-date>
<volume>33</volume>
<numero>1</numero>
<fpage>1</fpage>
<lpage>11</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0185-33092015000100001&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S0185-33092015000100001&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S0185-33092015000100001&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Usualmente, la identificación de especies de Meloidogyne está basada en la morfología de hembras adultas, lo que dificulta la identificación de hembras y machos juveniles (J2). Los nematodos son considerados entre los animales más difíciles de identificar, el uso de marcadores genéticos basados en el ADN ribosomal (ADNr) ha ganado aceptación en aplicaciones que van desde las determinaciones de cuarentena a las evaluaciones de la biodiversidad. Los nematodos del género Meloidogyne son conocidos por su habilidad para producir cambios fisiológicos en el sistema radical de las plantas y causar pérdidas en la absorción de nutrientes. El objetivo de este estudio fue determinar si la secuenciación de regiones espaciadoras transcritas (ITS) internas de ADNr pueden ser usadas como marcadores genéticos para la identificación confiable de poblaciones de hembras y machos juveniles (J2) para las principales especies del género Meloidogyne. De muestras de raíces enfermas de tomate (Solanum lycopersicum L.) se extrajeron larvas de hembras y machos juveniles de Meloidogyne para la extracción de ADN. Se amplificó una región génica del ADNr que contiene dos regiones ITS. Para su posterior secuenciación, dicha región se ligó al vector pGEM(r)-T. El análisis con el programa BLASTn indicó que la región génica presentó una identidad del 99.8 % respecto a una secuencia génica perteneciente a Meloidogyne incognita Kofoid &amp;amp; White, 1919. Tal resultado sugiere que las regiones ITS pueden ser usadas como un marcador genético en poblaciones de Meloidogyne para la identificación de especie.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Usually the identification of the Meloidogyne species is based on the morphology of adult females, making it difficult to identify juvenile males and females (J2). Nematodes are considered among the most difficult animals to identify; the use of ribosomal DNA (rDNA) based diagnostic methods have gained acceptance in applications ranging from quarantine determinations to assessments of biodiversity. Nematodes of the genus Meloidogyne are known for their ability to produce physiological changes in the root system of plants and cause losses in the absorption of nutrients. The objective of this study was to determine if the sequencing of internal transcribed spacer (ITS) regions of rDNA can be used as genetic markers for reliable identification of populations of juvenile males and females (J2) for the main species of the genus Meloidogyne. From samples of diseased tomato roots (Solanum lycopersicumL.), larvae of juvenile females and males of Meloidogyne were collected for the DNA extraction. A rDNA region harboring two ITS regions was amplified. For subsequent sequencing, that region was ligated into pGEM(r)-T vector. Analysis with the BLASTn program showed that the gene region identified 99.8 % with a gene sequence belonging to Meloidogyne incognita Kofoid &amp;amp; White, 1919. This result suggests that the ITS regions can be used as a genetic marker in populations for Meloidogyne species identification.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Meloidogyne incognit]]></kwd>
<kwd lng="es"><![CDATA[ITS]]></kwd>
<kwd lng="es"><![CDATA[ADN ribosomal (ADNr)]]></kwd>
<kwd lng="en"><![CDATA[Meloidogyne incognita]]></kwd>
<kwd lng="en"><![CDATA[ITS]]></kwd>
<kwd lng="en"><![CDATA[Ribosomal DNA (rDNA)]]></kwd>
</kwd-group>
</article-meta>
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