<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2448-6132</journal-id>
<journal-title><![CDATA[Abanico veterinario]]></journal-title>
<abbrev-journal-title><![CDATA[Abanico vet]]></abbrev-journal-title>
<issn>2448-6132</issn>
<publisher>
<publisher-name><![CDATA[Sergio Martínez González]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2448-61322021000100404</article-id>
<article-id pub-id-type="doi">10.21929/abavet2021.31</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Desarrollo y validación de dos inmunoensayos para la detección de Brucella canis en perros]]></article-title>
<article-title xml:lang="en"><![CDATA[Development and validation of two immunoassays for the detection of Brucella canis in dogs]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Palacios-Rodríguez]]></surname>
<given-names><![CDATA[Victor]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Benítez-Guzmán]]></surname>
<given-names><![CDATA[Alejandro]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Morales-Aguilar]]></surname>
<given-names><![CDATA[Aldo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Suárez-Güemes]]></surname>
<given-names><![CDATA[Francisco]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Nacional Autónoma de México Facultad de Medicina Veterinaria y Zootecnia ]]></institution>
<addr-line><![CDATA[Ciudad de México ]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2021</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2021</year>
</pub-date>
<volume>11</volume>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S2448-61322021000100404&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S2448-61322021000100404&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S2448-61322021000100404&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN: La brucelosis canina es causa de falla reproductiva y la detección temprana en los perros infectados sigue siendo un desafío. El objetivo del presente estudio fue probar dos antígenos para ser utilizados en dos diferentes inmunoensayos, para la detección de infección por Brucella canis en perros: uno a partir de antígeno crudo sonicado de Brucella canis RM6/66 (AC-iELISA) y otro utilizando la proteína inmunodominante GroEL (GroEL-iELISA). Se determinó el punto de corte usando sueros de perros infectados; posteriormente, se midió la reproducibilidad y se obtuvo un coeficiente de variación (CV) debajo de 15 % para negativos y positivos con el AC-iELISA, mientras que, para el GroEL-iELISA fueron superiores a 15 %. En la prueba de robustez, hubo diferencias significativas para el GroEL-iELISA, más no para el AC-iELISA. La prueba de selectividad mostró reacción cruzada con Leptospira interrogans en el AC-iELISA, y con L. interrogans y Salmonella spp. en el GroEL-iELISA. A partir de las pruebas de la estabilidad de la muestra se demostró que éstas deben almacenarse a temperatura ambiente no más de 2 horas, a 4 ºC no más de 24 horas y pueden mantenerse a -20 ºC hasta 30 días. Finalmente, se calcularon la sensibilidad y especificidad, resultando 100 % ambas para el AC-iELISA; una sensibilidad de 44 % y especificidad de 67 % para el GroEL-iELISA. En conclusión, el AC-iELISA demostró ser mejor en detectar a Brucella canis que el GroEL-iELISA.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT: Canine brucellosis is a cause of reproductive failure and early detection in infected dogs remains a challenge. The aim of the present study was to test two antigens to be used in two different immunoassays for the detection of Brucella canis infection in dogs: one from Brucella canis RM6/66 sonicated crude antigen (CA-iELISA) and the other using the immunodominant protein GroEL (GroEL-iELISA). The cut-off point was determined using sera from infected dogs; subsequently, reproducibility was measured and a coefficient of variation (CV) below 15 % was obtained for negatives and positives with the CA-iELISA, whereas, for the GroEL-iELISA they were higher than 15 %. In the robustness test, there were significant differences for the GroEL-iELISA, but not for the CA-iELISA. The selectivity test showed cross-reactivity with Leptospira interrogans in the CA-iELISA, and with L. interrogans and Salmonella spp. in the GroEL-iELISA. From the sample stability tests, it was demonstrated that samples should be stored at room temperature for no more than 2 hours, at 4 ºC for no more than 24 hours and can be kept at -20 ºC for up to 30 days. Finally, sensitivity and specificity were calculated, resulting in 100 % for both CA-iELISA; sensitivity of 44 % and specificity of 67 % for GroEL-iELISA. In conclusion, the CA-iELSA proved to be better at detecting Brucella canis than the GroEL-iELISA.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Brucella canis]]></kwd>
<kwd lng="es"><![CDATA[Brucelosis canina]]></kwd>
<kwd lng="es"><![CDATA[GroEL]]></kwd>
<kwd lng="es"><![CDATA[ELISA]]></kwd>
<kwd lng="en"><![CDATA[Brucella canis]]></kwd>
<kwd lng="en"><![CDATA[Canine Brucellosis]]></kwd>
<kwd lng="en"><![CDATA[GroEL]]></kwd>
<kwd lng="en"><![CDATA[ELISA]]></kwd>
</kwd-group>
</article-meta>
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