Stability of lycopene in cv. Saladette tomatoes (Lycopersicon esculentum Mill.) stored under differente conditions
Estabilidad de licopeno en tomates cv. Saladette (Lycopersicon esculentum Mill.) sujetos a distintas condiciones de almacenamiento
R.M. Galicia1*, R. Verde1, E. Ponce1, R.O. González2, C. Saucedo3 and I. Guerrero1
1 Departamento de Biotecnología, Universidad Autónoma Metropolitana–lztapalapa. Apartado postal 55–535, C.P. 09340 México, D.F., México. *Corresponding author. E–mail: rmgc@xanum.uam.mx Tel: (55) 5804 4711, Fax: (55) 5804 4712
2 Departamento de Matemáticas, Universidad Autónoma Metropolitana – lztapalapa. Apartado postal 55–535, C.P. 09340 México, D.F., México.
]]> 3 Programa de Fruticultura, Colegio de Postgraduados. Carretera México–Texcoco km 36.5. 56230. Montecillo, Estado de México, México.
Received 4th of October 2007
Accepted 6th of November 2008
Abstract
The objectives of this study were to determine the concentration of lycopene in cv. Saladette tomato subjected to blanching (thermal treatment), to extract the carotenoid and to evaluate the stability of the pigment in solutions added with and without antioxidants, in conditions of darkness at 4, 20 and 60 °C, and in fluorescent light at 20 °C during 30 days. The concentration of lycopene in non blanched tomatoes was 79.20 μg/g, while in blanched tomatoes it was 75.25 μg/g, none presenting any significant difference. The degradation of lycopene in solution during storage in darkness, fluorescent light or different temperatures, followed a reaction of the first order. In conditions of darkened or lightened storing at 20 °C, the retention of the carotenoid in both experiments was greater for solutions containing lycopene extracted from blanched tomatoes than for solutions with pigment extracted from unblanched tomatoes after 30 days. When the pigment in solution with antioxidants added was stored at different temperatures, the greatest lycopene retention was in darkness at 4 °C. In contrast, pigment degradation was greater in conditions of light and 20 °C, than in darkness at 60 °C. The constant rate of lycopene degradation increased when elevating temperature.
Keywords: tomato, lycopene, blanching, storage stability, kinetics.
Resumen
]]> Los objetivos de este estudio fueron determinar la concentración de licopeno en tomate cv. Saladette sometido a escaldado (tratamiento térmico), extraer el carotenoide y evaluar la estabilidad del pigmento en soluciones con y sin adición de antioxidantes, almacenadas en oscuridad a 4, 20 y 60 °C y en luz fluorescente a 20 °C durante 30 días. La concentración de licopeno en tomates sin escaldar fue de 79.20 μg/g mientras que para los tomates escaldados fue de 75.25 μg/g, no se presentó diferencia significativa. La degradación del licopeno en solución durante su almacenamiento en oscuridad, en luz fluorescente o a diferentes temperaturas siguió una reacción de primer orden. En condiciones de almacenamiento en oscuridad o en luz a 20 °C, la retención del carotenoide en ambos experimentos fue mayor para las soluciones con licopeno proveniente de tomates escaldados que para soluciones con pigmento de tomates no escaldados después de 30 días. Cuando el pigmento en solución con antioxidantes se almacenó a diferentes temperaturas, la mayor retención de licopeno fue en oscuridad a 4 °C. En condiciones de luz y 20 °C la degradación del pigmento fue mayor que en oscuridad y 60 °C. La constante de velocidad de degradación del licopeno se incrementó al elevar la temperatura.Palabras clave: tomate, licopeno, escaldado, estabilidad en almacenamiento, cinéticas.
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Acknowledgment
The author, Galicia Cabrera, wishes to thank the Consejo Nacional de Ciencia y Tecnología (CONACyT) in Mexico for her scholarship for graduate studies.
References
Akanbi, C.T. and Oludemi, F.O. (2004). Effect of processing and packaging on the lycopene content of tomato products. International Journal of Food Properties 7(1), 139–152. [ Links ]
Anese, M., Falcone, P., Fogliano, V., Nicoli, M.C. and Massini, R. (2002). Effect of equivalent thermal treatments on the color and the antioxidant activity of tomato purees. Journal of Food Science 67(9), 3442–3446. [ Links ]
Anguelova T. and Warthesen, J. (2000). Lycopene stability in tomato powders. Journal of Food Science 65(1), 67–70. [ Links ]
AOAC. (1996). Official Methods of Analysis of AOAC International, sixteenth edition, Vol. II. Association of Official Analytical Chemists, USA. Chapter 37, Pp. 5–6 [ Links ]
Barringer, S. (2004). Frozen tomatoes. In: Handbook of vegetable preservation and processing, (Hiu, Y.H., Ghazala, S., Graham, D.M. and Nip, W.K.,eds.). Pp. 293–308. Marcel Dekker, Inc. New York, [ Links ]
Boileau, T.W.M., Boileau, A.C. and Erdman, J.W. (2002). Bioavailability of all–trans and cis isomers of lycopene. Experimental Biology and Medicine 227(10), 914–919. [ Links ]
]]>Bouvier, F., Dogbo, O. and Camara, B. (2003). Biosynthesis of the food and cosmetic plant pigment bixin (annatto). Science 300, 2089-2091. [ Links ]
Britton, G. (1996). Carotenoids. In: Natural food colorants, (Hendry GF Houghton JD, ed.). Pp. 197–243. Blackie Academic and Professional, Glasgow, Scotland. [ Links ]
Bruno, A.K. and Wetzel, C.M. (2004). The early light–inducible protein (ELIP) gene is expressed during the chloroplast–to–chromoplast transition in ripening tomato fruit. Journal of Experimental Botany 55(408), 2541–2548. [ Links ]
Calvo, M. M., Dado, D. and Santa–María, G. (2007). Influence of extraction with ethanol or ethyl acetate on the yield of lycopene, P–carotene, phytoene and phytofluene from tomato peel powder. European Food Research and Technology 224, 567–571. [ Links ]
Candelas–Cadillo, M.G., Alanís–Guzmán, M.G.J., Bautista–Justo, M., Del Río–Olague and García–Díaz, C. (2005). Contenido de licopeno en jugo de tomate secado por aspersión. Revista Mexicana de Ingeniería Química 4, 299–307. [ Links ]
]]>Cvetkovic, D. and Markovic, D. (2008). Stability of carotenoids toward UV–irradiation in hexane soluction. Journal of the Servian Chemical Society 73(1), 15–27. [ Links ]
Delgado–Vargas F. and Paredes–Lopez, O. (2003). Carotenoids as food colors. In: Natural colorants for food and nutraceutical uses, (Delgado–Vargas F, Paredes–Lopez O. editors). Pp. 143–153. CRC Press, Boca Raton, Fla. [ Links ]
Fellows, P. (2005). Blanching. In: Food processing technology: principles and practice, (Fellows, P. ed.). Pp. 201–209. Ellis Horwood Ltd.,Publisher, London, England. [ Links ]
Ferreira, J.E.M. and Rodriguez–Amaya, D.B. (2008). Degradation of lycopene and β–carotene in model systems and in lyophilized guava during ambient storage: kinetics, structure, and matrix effects. Journal of Food Science 78(8), C589–C594. [ Links ]
Goula, A.M., Adamopoulos, K.G., Chatzitakis, P.C. and Nikas, V.A. (2006). Prediction of lycopene degradation during a drying process of tomato pulp. Journal of Food Engineering 74, 37–46. [ Links ]
]]>Gouldson, M.J. and Warthesen, J.J. (1999). Stability and antioxidant activity de beta carotene in conventional and high oleic canola oil. Journal of Food Science 64(6), 996–999. [ Links ]
Hackett, M.M., Lee, J.H., Francis, D. and Schwartz, S.J. (2004). Thermal stability and isomerization of lycopene in tomato oleoresins from different varieties. Journal of Food Science 69(7), 536–541. [ Links ]
Humphrey, A.M. (2004). Chlorophyll as a color and functional ingredient. Journal of Food Science 69(5), 422–425. [ Links ]
Ishida, B.K. and Chapman, M.H. (2004). A comparison of carotenoid content and total antioxidant activity in catsup from several commercial sources in the United States. Journal of Agricultural and Food Chemistry 52(26), 8017–8020. [ Links ]
Ishida, B.K., Roberts, J.S., Chapman, M.H. and Burri, B.J. (2007). Processing tangerine tomatoes: effects on lycopene–isomer concentrations and profile. Journal of Food Science 72(6), 307–312. [ Links ]
]]>Lee, M. T. and Chen B.H. (2001). Separation of lycopene and its cis isomers by liquid chromatography. Chromatographia 54, 613–617. [ Links ]
Lee, M. T. and Chen B.H. (2002). Stability of lycopene during heating and illumination in a model system. Food Chemistry 78, 425–432. [ Links ]
Lin, C.H. and Chen, B.H. (2005). Stability of carotenoids in tomato juice during processing. European Food Research and Technology 221, 274–280. [ Links ]
López–Hernández, E., Ponce–Alquicira, E., Cruz–Sosa, F. and Guerrero–Legarreta, I. (2001). Characterization and stability of pigments extracted from Terminalia catappa leaves. Journal of Food Science 66(6), 832–836. [ Links ]
Macrae, R., Robinson, R.K. and Sadler, M.J. (1993). Colours. In: Encyclopaedia of Food Science, Food Technology and Nutrition, vol. 2, Pp. 1164–1180. Academic Press, London, England. [ Links ]
]]>Mayeaux, M., Xu, Z., King, J.M. and Prinyawiwatkul, W. (2006). Effects of cooking condictions on the lycopene content in tomatoes. Journal of Food Science 71 (8), C461–C464. [ Links ]
Mencarelli F. and Saltveit, M. (1988). Ripening of mature–green tomato fruits slices. Journal of the American Society for Horticultural Science 113, 742–745. [ Links ]
Moraru, C. and Lee, T.C. (2005). Kinetic studies of lycopene isomerization in a tributyrin model system at gastric pH. Journal of Agricultural and Food Chemistry 53(23), 8997–9004. [ Links ]
Nelson, N. (1944). A photometric adaptation of the Somogyi method for the determination of glucosa. Journal of Biological Chemistry 153(2), 375–378. [ Links ]
Nguyen, M. L. and Schwartz, S. J. (1998). Lycopene stability during food processing. Proceedings of the Society for Experimental Biology and Medicine 218, 101–105. [ Links ]
]]>Nguyen, M.L. and Schwartz, S.J. (1999). Lycopene: chemical and biological properties, Food Technolgy 53(2), 38–45. [ Links ]
Nguyen, M.L. and Schwartz, S.J. (2000). Lycopene. In: Natural food colorants, (Lauro, J.G., Francis, F.J., editors). Pp. 153–192. Marcel Dekker, New York. [ Links ]
Re, R., Bramley, P.M. and Rice–Evans, C. (2002). Effects of Food processing on flavonoids and lycopene status in a Mediterranean tomato variety. Free Radical Research 36(7), 803-810. [ Links ]
Robinson W.B. and Stotz E. (1954). The indophenol–xileno extraction method for ascorbic acid. Journal of Biological Chemistry 160, 217-221. [ Links ]
Sadler, G., Davis, J. and Dezman, D. (1990). Rapid extraction of lycopene and P–caroteno from reconstituted tomato paste and pink grapefruit homogenates. Journal of Food Science 55(5), 1460–1461. [ Links ]
]]>Schwartz, S.T. (1994). Pigment analysis. In: Nielsen, S. Introduction to the chemical analysis of food. Pp. 261–271. Jones and Bartlett Publishers International. New York. [ Links ]
Servicio de Información y Estadística Agroalimentaria y Pesquera (SIAP). (2007). Anuario estadístico de la producción agrícola, 2005. Ciudad de México, México: SIAP–SAGARPA. p 63–66. [ Links ]
Shi, J. and Le Maguer, M. (2000). Lycopene in tomatoes: chemical and physical properties affected by food processing. Critical Reviews in Food Science and Nutrition 40(1), 1–42. [ Links ]
Shi, J., Le Maguer, M., Bryan, M. and Kakúda, Y. (2003) . Kinetics of lycopene degradation in tomato puree by heat and light irradiation. Journal of Food Process Engineering 25, 485–498. [ Links ]
Shi, J., Qu, Q., Kakúda, Y., Yeung, D. and Jiang, Y. (2004) . Stability and synergistic effect of antioxidative properties of lycopene and other active components. Critical Reviews in Food Science and Nutrition 44, 559–573. [ Links ]
]]>Sistema Nacional de información e Integración de Mercados (SNIIM). (2007). Consumo nacional aparente de productos agrícolas seleccionados, 2007. Ciudad de México, México: SNIIM–SE. 3p. [ Links ]
Spagna, G., Barbagallo, R.N., Chisari, M. and Branca, F. 2005. Characterization of a tomato polyphenol oxidase and its role in browning and lycopene content. Journal of Agricultural and Food Chemistry 53, 2032–2038. [ Links ]
Takeoka, G.R., Dao, L., Flessa, S., Gillespie, D.M., Jewell, W.T., Huebner, B., Bertow, D. and Ebeler, S.E. (2001). Processing effects on lycopene content and antioxidant activity of tomatoes. Journal of Agricultural and Food Chemistry 49(8), 3713–3717. [ Links ]
Thompson, K.A., Marshall, M.R., Sims, C.A., Wei, C.I., Sargent, S.A. and Scott, J.W. (2000). Cultivar, maturity, and heat treatment on lycopene content in tomatoes. Journal of Food Science 65(5), 791–795. [ Links ]
Wrolstad, R.E. (2004). Anthocyanin pigments: bioactivity and coloring properties. Journal of Food Science 69(5), 419–421. [ Links ]
]]>Xianquan, S., Shi, J., Kakuda, Y. and Yueming, J. (2005) . Stability of lycopene during food processing and storage. Journal of medicinal food 8(4), 413–422. [ Links ]
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