<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0187-7380</journal-id>
<journal-title><![CDATA[Revista fitotecnia mexicana]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. fitotec. mex]]></abbrev-journal-title>
<issn>0187-7380</issn>
<publisher>
<publisher-name><![CDATA[Sociedad Mexicana de Fitogenética A.C.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0187-73802022000400493</article-id>
<article-id pub-id-type="doi">10.35196/rfm.2022.4.493</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Regeneración in vitro de Agave wocomahi Gentry (Asparagaceae)]]></article-title>
<article-title xml:lang="en"><![CDATA[In vitro regeneration of Agave wocomahi Gentry (Asparagaceae)]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Reyes-Silva]]></surname>
<given-names><![CDATA[Alberto I.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Nuñez-Palenius]]></surname>
<given-names><![CDATA[Héctor Gordon]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ocampo]]></surname>
<given-names><![CDATA[Gilberto]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Pérez-Molphe-Balch]]></surname>
<given-names><![CDATA[Eugenio]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Autónoma de Aguascalientes Departamento de Química Centro de Ciencias Básicas]]></institution>
<addr-line><![CDATA[Aguascalientes Aguascalientes]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad de Guanajuato Departamento de Agronomía División de Ciencias de la Vida]]></institution>
<addr-line><![CDATA[Irapuato Guanajuato]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Universidad Autónoma de Aguascalientes Departamento de Biología Centro de Ciencias Básicas]]></institution>
<addr-line><![CDATA[Aguascalientes Aguascalientes]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2022</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2022</year>
</pub-date>
<volume>45</volume>
<numero>4</numero>
<fpage>493</fpage>
<lpage>501</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0187-73802022000400493&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S0187-73802022000400493&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S0187-73802022000400493&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen Agave wocomahi es una especie con potencial de ser aprovechada para diversos fines; sin embargo, la baja eficiencia de sus sistemas naturales de propagación hace que no haya ejemplares disponibles, y la colecta de plantas silvestres pondría bajo amenaza a las poblaciones naturales. Se desarrollaron protocolos para el cultivo y propagación in vitro de esta especie. Se indujo la brotación de meristemos laterales cultivando segmentos de tallo en medio basal con 1 mg L-1 de 6-bencilaminopurina (BA); posteriormente, se determinaron las respuestas morfogénicas de explantes de tallo, hoja y raíz. Se establecieron tratamientos con ácido naftalenacético (ANA), ácido indolacético (AIA), ácido 4-amino-3,5,6-tricloropicolínico (PIC) y ácido 2,4-diclorofenoxiacético (2,4-D) en concentraciones de 1.5 y 4.0 mg L-1, solas o en combinación con 1.5 y 3.0 mg L-1 de BA. Se observó la formación de cinco tipos de tejido calloso (TC), de los cuales, el tejido nodular se generó mayormente en explantes de tallo y hoja, y el TC con raíces en explantes de raíz. Los tratamientos con PIC solo, o combinado con BA, generaron una mayor inducción de callo (99.16 %) en explantes de tallo, prevaleciendo el TC nodular. Se generaron brotes adventicios mediante organogénesis directa e indirecta en los tratamientos con AIA; además, se observaron indicios de embriogénesis somática indirecta en callo generado con 1.5 mg L-1 de AIA con 1.5 y 3.0 mg L-1 de BA, con BA solo y en combinación con 2,4-D. El desarrollo de los presuntos embriones hasta plántulas fue favorecido al retirarse los reguladores de crecimiento vegetal y adicionarse 1 g L-1 de carbón activado al medio. Las plantas generadas se adaptaron a las condiciones ex vitro con una supervivencia superior al 90 %. Estos protocolos de multiplicación in vitro podrían convertirse en una herramienta para el aprovechamiento sostenible de la especie.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Summary Agave wocomahi is a species with the potential to be exploited for various purposes; however, the low efficiency of their natural propagation systems means that there are no specimens available, and the harvesting of wild plants would threaten natural populations. Protocols for in vitro culture and propagation of this species were developed. Budding of lateral meristems was induced by culturing stem segments in basal medium with 1 mg L-1 of 6-benzyl amino purine (BA); subsequently, the stem, leaf, and root morphogenic responses explants were determined. Treatments were established with naphthaleneacetic acid (NAA), indoleacetic acid (IAA), 4-amino-3,5,6-trichloropicolinic acid (PIC) and 2,4-dichlorophenoxyacetic acid (2,4-D), in concentrations of 1.5 and 4.0 mg L-1, alone or combined with 1.5 and 3.0 mg L-1 of BA. The formation of five types of callous tissue (TC) was observed, of which the nodular tissue was generated mainly in stem and leaf explants and the TC with roots in root explants. Treatments with PIC, alone or combined with BA, caused higher callus induction (99.16 %) in stem explants, with nodular TC prevailing. Adventitious shoots were generated by direct and indirect organogenesis in the IAA treatments; furthermore, indirect somatic embryogenesis was observed in callus generated with 1.5 mg L-1 IAA with 1.5 and 3.0 mg L-1 BA, with BA alone and in combination with 2,4-D. The development of the presumed embryos into seedlings was favored by removing the plant growth regulators and adding 1 g L-1 of activated charcoal to the medium. The plants generated adapted to ex vitro conditions with a survival greater than 90 %. These in vitro multiplication protocols could become a tool for the sustainable use of the species.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Agave wocomahi]]></kwd>
<kwd lng="es"><![CDATA[embriogénesis somática]]></kwd>
<kwd lng="es"><![CDATA[organogénesis]]></kwd>
<kwd lng="es"><![CDATA[tejido calloso]]></kwd>
<kwd lng="en"><![CDATA[Agave wocomahi]]></kwd>
<kwd lng="en"><![CDATA[callus tissue]]></kwd>
<kwd lng="en"><![CDATA[organogenesis]]></kwd>
<kwd lng="en"><![CDATA[somatic embryogenesis]]></kwd>
</kwd-group>
</article-meta>
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