<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0187-7380</journal-id>
<journal-title><![CDATA[Revista fitotecnia mexicana]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. fitotec. mex]]></abbrev-journal-title>
<issn>0187-7380</issn>
<publisher>
<publisher-name><![CDATA[Sociedad Mexicana de Fitogenética A.C.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0187-73802019000400439</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[IDENTIFICACIÓN DE cDNAs DE Agave tequilana Weber Var. azul SIMILARES A FUROSTANOL GLICÓSIDO 26-O-&#946;-GLUCOSIDASAS]]></article-title>
<article-title xml:lang="en"><![CDATA[IDENTIFICATION OF cDNAs FROM Agave tequilana Weber Var. azul SIMILAR TO FUROSTANOL GLYCOSIDE 26-O-&#946;-GLUCOSIDASES]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Monroy-González]]></surname>
<given-names><![CDATA[Zurisadaí]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Martínez-Hernández]]></surname>
<given-names><![CDATA[Aída]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Colegio de Postgraduados  ]]></institution>
<addr-line><![CDATA[Champotón Campeche]]></addr-line>
<country>México</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Colegio de Postgraduados  ]]></institution>
<addr-line><![CDATA[Champotón Campeche]]></addr-line>
<country>México</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2019</year>
</pub-date>
<volume>42</volume>
<numero>4</numero>
<fpage>439</fpage>
<lpage>447</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0187-73802019000400439&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S0187-73802019000400439&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S0187-73802019000400439&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen La furostanol glicósido 26-O-&#946;-glucosidasa (F26G) participa en el último paso de la biosíntesis de saponinas esteroidales, lo que escinde la glucosa unida al hidroxilo del C26 del furostanol glucósido para permitir la formación del espirostano. A pesar de la existencia de numerosos estudios que muestran la diversidad estructural y actividad biológica de las saponinas de plantas, así como su reconocido potencial biotecnológico y farmacológico, pocas enzimas participantes en la ruta de biosíntesis de las saponinas esteroidales han sido estudiadas y sólo una enzima nativa tipo F26G ha sido purificada y caracterizada funcionalmente. En este estudio, mediante búsqueda por BLASTX en una base de datos de Agave tequilana Weber var. azul (Agave DB), se identificaron 46 ESTs (Expressed Sequence Tags) de DNA complementario (cDNAs) similares a la F26G de Asparagus officinalis, la mayoría provienen de cDNAs clonados a partir de tejidos florales (anteras y ovarios) o raíz; ambos órganos son productores de saponinas. Entre ellos, se identificaron seis ESTs cuyo alineamiento indica que representan a tres cDNAs diferentes entre sí. Estos ESTs se registraron en la base de datos dbEST de NCBI (National Center for Biotechnology Information), el cual fue el primer registro de secuencias nativas de cDNAs potencialmente codificantes similares a F26G en Agave. El tamaño de los cDNAs clonados y su alineamiento hacia el lado 5&#8217; de las F26G conocidas sugieren que son cDNAs completos. Se diseñaron iniciadores diferenciales específicos para cada tipo de cDNA y se analizó por RT-PCR su expresión en tejidos vegetativos de A. tequilana. Se establecieron protocolos de PCR cuantitativo (qPCR) para estudios posteriores de regulación de su expresión génica. La información aquí reportada servirá como base para estudiar la función, regulación y actividad enzimática de las enzimas clonadas de Agave similares a F26G.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract Furostanol glycoside 26-O-&#946;-glucosidase (F26G) participates in the last step of steroidal saponin biosynthesis, cleaving the glucose bound to the C26 of the hydroxylin furostanol glucoside to allow the formation of spirostane. Despite the existence of numerous studies showing the structural diversity and biological activity of plant saponins, as well as their recognized biotechnological and pharmacological potential, few enzymes involved in the biosynthetic pathway of steroidal saponins have been studied and only one native F26G enzyme has been isolated and functionally characterized. In this study, by searching a database of Agave tequilana Weber var. azul (Agave DB), 46 ESTs (Expressed Sequence Tags) of complementary DNA (cDNAs) similar to the known F26G were identified through BLASTX, most come from cDNA from floral tissues (anthers and ovaries) or roots; both organs are saponins producers. Among them, six ESTs were identified whose alignment indicates that they represent three cDNAs different from each other. These ESTs were submitted to the dbEST database of NCBI (National Center for Biotechnology Information), being this the first record of native cDNA sequences putatively encoding F26G-like in Agave. The size of the cloned cDNAs and their alignment to the 5&#8217; end of the known F26G suggest they are full-length cDNAs. Specific differential primers were designed for each type of cDNA and their expression in vegetative tissues of A. tequilana was analyzed by RT-PCR. Quantitative PCR protocols (qPCR) were established for future studies on gene expression regulation. The information reported here will serve as a basis for studying the function, regulation and enzymatic activity of F26G-like cloned from Agave.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Agave tequilana]]></kwd>
<kwd lng="es"><![CDATA[cDNA]]></kwd>
<kwd lng="es"><![CDATA[EST]]></kwd>
<kwd lng="es"><![CDATA[furostanol glicósido 26-O-&#946;-glucosidasa]]></kwd>
<kwd lng="es"><![CDATA[saponinas]]></kwd>
<kwd lng="en"><![CDATA[Agave tequilana]]></kwd>
<kwd lng="en"><![CDATA[cDNA]]></kwd>
<kwd lng="en"><![CDATA[EST]]></kwd>
<kwd lng="en"><![CDATA[furostanol glycoside 26-O-&#946;-glucosidase]]></kwd>
<kwd lng="en"><![CDATA[saponins]]></kwd>
</kwd-group>
</article-meta>
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