<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0185-3309</journal-id>
<journal-title><![CDATA[Revista mexicana de fitopatología]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. mex. fitopatol]]></abbrev-journal-title>
<issn>0185-3309</issn>
<publisher>
<publisher-name><![CDATA[Sociedad Mexicana de Fitopatología A.C.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0185-33092023000400106</article-id>
<article-id pub-id-type="doi">10.18781/r.mex.fit.2023-5</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Comparación de protocolos moleculares para la detección del Virus del fruto rugoso marrón del jitomate en hospedantes solanáceos]]></article-title>
<article-title xml:lang="en"><![CDATA[Comparison of molecular protocols to detect Tomato brown rugose fruit virus in solanaceae hosts]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Zamora-Macorra]]></surname>
<given-names><![CDATA[Erika J.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Aviña-Padilla]]></surname>
<given-names><![CDATA[Katia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Hammond]]></surname>
<given-names><![CDATA[Rosemarie W]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ochoa-Martínez]]></surname>
<given-names><![CDATA[Daniel L]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Autónoma Chapingo  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Instituto Politécnico Nacional Centro de Investigación y de Estudios Avanzados ]]></institution>
<addr-line><![CDATA[Irapuato ]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,United States Department of Agriculture Agricultural Research Service ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>USA</country>
</aff>
<aff id="Af4">
<institution><![CDATA[,Colegio de Postgraduados  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2023</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2023</year>
</pub-date>
<volume>41</volume>
<numero>spe</numero>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0185-33092023000400106&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S0185-33092023000400106&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S0185-33092023000400106&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen Objetivo/Antecedentes: El virus del fruto rugoso marrón del jitomate (ToBRFV) ha surgido como una amenaza significativa para los cultivos de la familia Solanaceae, incluidos el tomate y el pimiento. Su presencia en México desde 2018 ha generado preocupación sobre su impacto en la producción agrícola. La detección temprana y precisa de este patógeno es crucial para prevenir su propagación y mitigar sus efectos. En México, se emplean varias técnicas moleculares para su diagnóstico, incluyendo RT-PCR convencional, RT-qPCR y RT-qPCR multiplex. Materiales y Métodos: El objetivo de esta investigación fue evaluar la eficiencia de diferentes métodos de extracción de ARN en combinación con oligonucléotidos PCR específicos para la detección de ToBRFV. Resultados: Entre los métodos probados, el protocolo de extracción de ARN CTAB-Trizol combinado con PCR anidada utilizando oligonucleótidos reportados por Dovas et al. (2004) se identificó como el método molecular más sensible para detectar el virus. Conclusión: Este hallazgo destaca la importancia de seleccionar la combinación adecuada de protocolos de extracción y amplificación para lograr la sensibilidad y precisión óptimas en la detección de ToBRFV.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract  Objective/Background: The Tomato brown rugose fruit virus (ToBRFV) has emerged as a significant threat to Solanaceae crops, including tomato and pepper. Its presence in Mexico since 2018 has raised concerns about its impact on agricultural production. Early and accurate detection of this pathogen is essential to prevent its spread and mitigate its effects. In Mexico, several molecular techniques are employed for its diagnosis, including endpoint RT-PCR, RT-qPCR, and multiplex RT-qPCR.  Materials and Methods: This research aimed to assess the efficiency of different RNA extraction methods in combination with specific PCR primers for detecting ToBRFV.  Results: Among the methods tested, the CTAB-Trizol RNA extraction protocol combined with nested PCR using primers reported by Dovas et al. (2004) was identified as the most sensitive molecular method for detecting the virus.  Conclusion: This finding highlights the importance of selecting the appropriate combination of extraction and amplification protocols to achieve optimal sensitivity and accuracy in ToBRFV detection.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Virus del fruto rugoso marrón del jitomate]]></kwd>
<kwd lng="es"><![CDATA[ToBRFV]]></kwd>
<kwd lng="es"><![CDATA[cultivos de Solanaceae]]></kwd>
<kwd lng="es"><![CDATA[extracción de ARN]]></kwd>
<kwd lng="es"><![CDATA[RT-PCR]]></kwd>
<kwd lng="es"><![CDATA[detección molecular]]></kwd>
<kwd lng="es"><![CDATA[producción agrícola]]></kwd>
<kwd lng="en"><![CDATA[Tomato brown rugose fruit virus]]></kwd>
<kwd lng="en"><![CDATA[ToBRFV]]></kwd>
<kwd lng="en"><![CDATA[Solanaceae crops]]></kwd>
<kwd lng="en"><![CDATA[RNA extraction]]></kwd>
<kwd lng="en"><![CDATA[RT-PCR]]></kwd>
<kwd lng="en"><![CDATA[molecular detection]]></kwd>
<kwd lng="en"><![CDATA[agricultural production]]></kwd>
</kwd-group>
</article-meta>
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