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Veterinaria México OA

versión On-line ISSN 2448-6760

Veterinaria México OA vol.5 no.4 México oct./dic. 2018

http://dx.doi.org/10.22201/fmvz.24486760e.2018.4.470 

Original Research Articles

Addition of calcium propionate to finishing lamb diets

José A. Martínez-Aispuro1  * 

María T. Sánchez-Torres1  * 

Germán D. Mendoza-Martínez2 

José L. Cordero-Mora1 

José L. Figueroa-Velasco1 

Marco A. Ayala-Monter3 

María M. Crosby-Galván1 

1Programa de Ganadería, Campus Montecillo, Colegio de Postgraduados, Texcoco, Estado de México, México.

2Departamento de Producción Agrícola y Animal, Universidad Autónoma Metropolitana Xochimilco, Ciudad de México, México.

3Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Guerrero, Cuajinicuilapa, Guerrero, México.

Abstract:

Calcium (Ca) propionate can be added to ruminant diets as a glucogenic substrate. However, due to its hypophagic effect, it is necessary to establish the optimal dose that can be used to replace grains in finishing diets for lambs. Therefore, the objective of this study was to assess the effect of four concentrations of Ca propionate in lamb diets on productive performance and rumen fermentation. Thirty two Hampshire x Suffolk lambs (23.82 ± 0.40 kg initial body weight), distributed in a completely randomized design, were given a diet with four concentrations of Ca propionate (g kg−1): 0, 10, 20 or 30 g, for 42 days. The results were tested for linear or quadratic responses. The final weight, average daily gain and feed: gain ratio showed quadratic responses (P ≤ 0.01). The optimal dose was established at 13.77 g kg−1 DM. Addition of Ca propionate did not affect variables related to rumen fermentation (pH, total volatile fatty acids, acetate, butyrate or rumen ammonia-N; P ≥ 0.05). Nonetheless, glucose and propionate concentrations showed a quadratic response (P ≤ 0.05). The highest concentrations of propionate in rumen were observed with 15.14 g kg−1 DM. Results indicate that Ca propionate can be included in a dose of up to 13.77 g kg−1 DM in feedlot rations, to attain best lamb performance.

Keywords: volatile fatty acids; glucose; metabolizable energy; calcium propionate; lambs

Introduction

Due to a constant increase in grain prices and to competition with sectors such as biofuel production or use for human consumption, it is necessary to find alternatives to partially replace the employ of grains in finishing animal rations. Calcium (Ca) propionate is mainly used as a preservative for animal feeds. It also has the potential to be added as an additive in sheep diets,1,2 since propionate is the most important gluconeogenic precursor for ruminants, and can improve the flow of glucose in lambs.3 In addition, supplementation of Ca propionate improves insulin action on glucose metabolism4 involved in fat deposition5 and muscle growth.1 However, there is a physiological limit to Ca propionate conversion to glucose7, and it should be added to ruminant diets in low doses due to its hypophagic effect.6

Ca propionate has been used as an energy substrate in lamb diets in a dose of up to 20 g kg−1 DM without adverse effects on productive variables or rumen fermentation.1,2 In fact, its use in steers improved rumen fermentation and feed digestibility,8 which could be a further benefit for its use as a nutrient for animal growth. In previous studies with feedlot lamb diets1,2 researchers have discussed the difference between expected and observed gain in relation to the energetic value of Ca propionate.

There are a few reports that specify the energy values of Ca propionate9,6,10 which can be used as a reference for diet formulation. However, animal response is required to accurately estimate the energy value, which should be based on increasing concentrations of Ca propionate. If the response is linear, a constant energy value can be considered, whereas if there is a quadratic effect (associative effects), the energetic value should be a function of the concentration of inclusion. Therefore, the objective of this study was to evaluate the effect of addition of 4 concentrations of Ca propionate to finishing diets of lambs on animal performance, rumen fermentation and blood glucose concentration.

Materials and methods

The experimental procedures were performed according to the International Guidelines for the use of animals in Biomedical Research,11 and complied with the official Mexican norm12 for care and use of laboratory animals. The study was conducted in the sheep facilities of the Experimental Farm at the Colegio de Postgraduados, located in Montecillo, State of Mexico (long. 98º 48’ 27’’ W and lat. 19º 48’ 23” N). The region is semi-arid, located at a mean altitude of 2,241 m, and has a temperate climate with a mean annual temperature of 15.9 °C, infrequent frosts and a mean annual rainfall of 686 mm.13

Animals and diets

Thirty two male Hampshire × Suffolk lambs with an initial BW of 23.8 ± 1.13 kg were included. Animals were housed in individual pens equipped with an exclusive feeder and an individual nipple drinker. Lambs were dewormed (Closantel, 20 mg kg−1 animal−1) and vaccinated (Bobact® 8, 2.0 ml animal−1) before the start of the study. Food was offered at 8:00 h and 15:00 h daily. Both food and water were provided ad libitum. The animals had a 10 d adaptation period to diets. The experimental phase lasted for 42 d. Treatments consisted of four different added concentrations of Ca propionate to diets (dry basis): 0, 10, 20 and 30 g kg−1 DM (Propical®, Dresen S. A. de C. V. Queretaro, México). Rations were formulated for a daily gain of 300 g d. Particular diet composition is presented in Table 1 1,14.

Table 1 Experimental diets for lambs fed different concentrations of calcium propionate 

Calcium propionate g kg−1 DM
Ingredient (%) 0 10 20 30
Corn grain 57.49 54.73 51.63 48.92
Soybean meal 9.00 10.61 12.54 14.00
Cane molasses 5.00 5.00 5.00 5.00
Calcium propionate 0.00 1.00 2.00 3.00
Alfalfa hay 26.80 20.07 11.93 6.24
Oat straw 0.20 6.93 15.07 20.76
Calcium carbonate 0.05 0.22 0.43 0.52
Calcium orthophosphate 0.46 0.43 0.40 0.55
Mineral premix 1.00 1.00 1.00 1.00
Calculated nutrient composition (DM basis)
Metabolizable energy (Mcal kg−1) 2.50 2.50 2.50 2.50
CP (%) 14.00 14.00 14.00 14.00
UIP§ (%) 5.60 5.64 5.70 5.71
Calcium (%) 0.69 0.69 0.69 0.70
Phosphorus (%) 0.50 0.50 0.50 0.50
ADF (%) 12.36 12.70 13.04 13.21
Determined nutrient composition (DM basis)
CP (%) 13.85 13.96 13.79 13.98
Calcium (%) 0.70 0.72 0.69 0.75
Phosphorus (%) 0.43 0.45 0.42 0.48
ADF (%) 13.15 13.26 13.54 13.70

† Mineral premix: Phosphorus 17.5 g/ 100g, Calcium 6.5 g/ 100g, Sodium 6.5 g/ 100g, Sulfur 2 g/ 100g.

CP: crude protein.

§UIP: rumen-undegradable protein.

Þ ADF, acid detergent fibre.

Growth assay

Recorded variables were: average daily feed intake, average daily gain (ADG), feed: gain ratio (FGR) and final body weight (FBW). Backfat thickness and chop area were measured between the 12th and 13th ribs using a Sonovet 600 real time ultrasound15 (Medison, Inc., Cypress, California, USA) fitted with a 7.5 Mhz transducer on days 1 and 42 of the study.

Chemical analysis

Diet crude protein content was determined by the macro kjeldahl method.16 Calcium and phosphorus levels were measured by atomic absorption spectrophotometry17 with a Perkin Elmer 4000 Model (Series Lambda 2, Perkin Elmer Inc., Norwalk, CT, USA). Acid detergent fiber was quantified by the Van Soest et al.18 procedure. Blood (5 mL) was collected on days 1 and 42 using vacutainer tubes without anticoagulant, and immediately placed under refrigeration (4 °C). Samples were centrifuged (Sigma 2-16k, Germany) at 3500 g x 20 min to obtain blood serum, which was stored in Eppendorf tubes and kept in a freezer (MDF-436 Sanyo, USA) at -20 °C until glucose determination.19

Rumen fermentation

Rumen fluid (50 mL) was extracted at 0800 hours with an esophageal tube, on day 42 of the trial (from animals fasted for 16 h). Rumen fluid was collected in an Erlenmeyer flask, immediately filtered with a double layer of gauze and transferred into a 100 mL beaker. A portable potentiometer was used to measure pH (Orion, mod A210, USA). Four mL of rumen fluid were then placed in a test tube with 1 mL of metaphosphoric acid (25 %) and stored in a freezer (-20 °C) for further analyses. The N-NH3 concentrations were determined following the McCullough20 procedure with a spectrophotometer (Cary 1-E Varian; Perkin Elmer, Model Lamda-40, USA) at 630 nm. Volatile fatty acid content (VFA) was determined by gas chromatography21 using a Perkin Claurus Elmer 500, with automatic-sampler.

Statistical analyses

Data were analyzed using the GLM procedure 22 for a completely randomized design, with four treatments and eight replicates, considering each lamb as an experimental unit. Shapiro-Wilk and Levene’s tests were used to verify normal dis tribution and variance homogeneity of data. Orthogonal contrasts were used to test linear or quadratic effects of Ca propionate concentrations in response variables of interest (P ≤ 0.05)., The best fitted model was determined by the smallest P value and the largest coefficient of determination (R2). Initial body weight was used as a covariate. For quadratic responses, the optimum Ca propionate concentration was estimated by linearization of the Gamma function.23

Results and discussion

Growth assay (Table 2)

Table 2 Performance of finishing lambs fed with different concentrations of calcium propionate 

Calcium propionate (g kg−1) SEMÞ P value
0 10 20 30 Linear Quadratic
Initial body weight (kg) 23.33 23.83 23.82 24.29 0.40 - -
Final body weight (kg) 34.93 37.28 36.99 35.24 0.69 0.41 **
ADG (g d−1) 0.29 0.34 0.33 0.27 0.01 0.41 **
ADFI (g d−1) 1.40 1.39 1.38 1.37 0.04 0.12 0.92
FGR§ 4.86 4.15 4.28 5.08 0.21 0.67 **
Chop area (mm2) 993.88 985.13 998.38 985.13 27.35 0.86 0.94
Backfat thickness (mm) 3.00 3.13 3.13 3.00 0.09 0.89 0.17

ADG: average daily gain;

ADFI: daily feed intake;

§FGR: feed: gain ratio;

SEM: standard error of the mean;

*(P ≤ 0.05);

**(P ≤ 0.01)

The average daily feed intake was not affected by the addition of Ca propionate to the diet (P > 0.05). The FBW and ADG showed a quadratic response (P < 0.01), estimating that the optimal added Ca propionate concentration for the enhancement of both of these performance variables is 13.77 g kg−1 (ADG = 0.2298235 + 0.0069876*Ca propionate - 0.0002538*Ca propionate2 + 0.0025842*IBW, R2= 0.36; FBW = 9.2194604+ 0.2799271*Ca propionate - 0.0101639*Ca propionate2 + 1.1020400*IBW, R2= 0.56). The FGR ratio also showed a quadratic response (P < 0.01) to Ca propionate (F:G = 2.2492769 - 0.1087659*Ca propionate + 0.0037852*Ca propionate2 + 0.1113845*IBW; R2 = 0.37). The chop area and backfat thickness were not affected by the concentration of Ca propionate in the diet (P > 0.05).

Previous studies have reported that the addition of up to 20 g kg−1 DM of Ca propionate to feedlot rations of lambs did not decrease feed intake.1,2,24In contrast, the use of Ca propionate in cattle seems to have a hypophagic effect25,26 when consumed in concentrations of over 12 moles per day−1.2 This suggests that the addition of Ca propionate may cause hypophagic effects when added in amounts greater than 30 g kg−1 DM. Nonetheless, concentrations over 30 g kg−1 DM were not included in this study.

Previous work has shown that 10 or 20 g kg−1 DM of Ca propionate added to finishing diets of lambs did not modify ADG or FGR.1,2 However, supply of propionate directly into the rumen, or use of dietary sources that increase propionate production in improve average daily gain in dairy cows.26, 27

Average daily gain measurements in this study showed that the energy value of Ca propionate is not constant and that it has a curvilinear nature similar to that observed in cows by Rigout et al.9 Based on the system equations net energy of California28 we can estimate a ME value for Ca propionate of 4.8 Mcal kg−1 DM when it is added to the diet in a concentration of 10 g kg−1 DM. Conversely, when it is included at higher concentrations, its estimated ME value can be reduced by half and or energy may not be produced at all. The ME value estimated in this study is higher than the one reported by Mendoza-Martinez et al.2 of 3.766 Mcal kg−1, which may be due to the fact that the value is not constant.

Lee-Rangel et al.1 and Mendoza-Martinez et al.2 observed that the addition of Ca propionate in finishing diets of lambs did not alter chop area or carcass weight. However, Mendoza-Martinez et al.2 observed a reduction of oleic acid and an increase of linolenic acid. Bbut backfat thickness was not measured in their experiment, but authors reported that Ca propionate did modify fatty acid composition of meat.

Rumen fermentation and blood glucose concentrations (Table 3)

Table 3 Rumen fermentation pattern and blood glucose concentrations in finishing lambs fed with diets containing different concentrations of calcium propionate 

Calcium propionate (g kg−1) SEMÞ P value
0 10 20 30 Linear Quadratic
Serum glucose (mg dL−1) 60.75 71.00 68.25 67.25 1.97 0.84 **
Rumen pH 7.15 7.35 7.22 7.30 0.05 0.43 0.22
Ammonia-N (mg dL−1) 4.06 3.26 4.43 3.98 0.93 0.99 0.96
Total VFA (mmol L−1) 18.96 21.19 18.20 20.07 1.82 0.91 0.86
VFA (mmol mmol−1 )
Acetate 0.63 0.61 0.57 0.62 0.017 0.25 0.19
Propionate 0.16 0.20 0.22 0.21 0.01 * *
Butyrate 0.14 0.12 0.12 0.11 0.01 0.19 0.26

VFA: volatile fatty acids.

SEM: standard error of the mean.

*(P ≤ 0.05);

**(P ≤ 0.01)

The addition of Ca propionate to the diet did not affect pH, total VFA, or N- ammonia concentrations in rumen fluid (P > 0.05). Similarly, levels of acetate and butyrate were not affected (P > 0.05). However, rumen fluid propionate concentrations showed a quadratic response (P < 0.05), reaching its highest level with an estimated Ca propionate concentration of 20.34 g kg−1 DM (Propionate = 0.1802155 + 0.0062904*Ca propionate - 0.0001546*Ca propionate2 + 0.0009891*IBW; R2 = 0.49). Blood glucose levels also showed a quadratic response (P < 0.01) to added dietary Ca propionate with the highest response reached with 15.14 g kg−1 (Glucose = 61.0171329 + 1.7246650*Ca propionate - 0.0569418*Ca propionate2 + 0.0411237*IBW, R2 = 0.78).

Quigley and Heitmann29 did not find changes on rumen fluid pH after Ca propionate infusions in sheep. Similarly, Lee-Rangel et al.1 and Mendoza-Martínez et al.2 did not observe rumen fluid pH or VFA alterations when adding Ca propionate to lamb diets.

In a previous study Sanchez et al.30 found that Ca propionate supplementation increased rumen propionate and reduced the acetate:propionate ratio without having an effect on pH or on total VFA in heifers. Moreover, an increase in propionate enhances weight gain in heifers 27as it relates with an increase of blood glucose levels.

In a study conducted by Majdoub et al.24 propionate supplementation to grazing lambs increased glucose production and lactate concentrations, the latter of which can also be used for additional glucose synthesis. These changes can be associated with an increase in insulin secretion.4,24 Accordingly, other studies with dairy cattle have shown that propionate, either infused or added to the diet, increases blood glucose concentrations.26,31

Implications

The increase of propionate and glucose concentrations following Ca propionate administration to finishing diets of lambs (13.77 g kg−1), improves average daily gain and FGR .However, when Ca propionate is supplemented at higher doses (20 g kg−1) the benefits of its use are reduced. Economic analyses may drive the use of Ca propionate in farms because even if the cost per kg ($ 5.0 US dollars kg−1) surpasses that of the cost of grain (1 kg corn $ 0.25 US dollars), the results show that 10 g kg−1 of Ca propionate can replace 150 g kg−1 of grains without affecting lamb performance.1

Conclusion

Ca propionate can be added as an energy source in finishing lamb diets in concentrations of up to 30 g kg-1 DM, without affecting lamb performance, carcass traits or rumen fermentation. According to this study, the recommended quantity to add to lamb finishing diets is 13.77 g kg-1 DM, based on improvement of performance variables. This dose also increased rumen propionate and serum glucose concentrations.

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Aprobado: 22 de Noviembre de 2018

* Corresponding author: Tel: +52 595 952 0200 ext. 75054 E-mail: teresa@colpos.mx

Conflicts of interest

Authors declare that there is no conflict of interest regarding publication of this article.

Author contributions

Jose Alfredo Martinez Aispuro: experimental work; laboratory work and statistical analysis of data. Drafted manuscript.

María Teresa Sánchez-Torres: designed, directed and supervised the research; revised final manuscript.

German David Mendoza Martinez: revised research proposal and final manuscript.

José Luis Cordero-Mora: collaborated with proposal draft, experimental work; supervised health status of lambs; obtained samples and data; revised final version of manuscript.

José Luis Figueroa-Velasco: revised research proposal, final version of proposal and final manuscript.

Marco Antonio Ayala Monter: experimental design; supervision of experimental protocol; supervision and support to experimental and data analysis.

Maria Magdalena Crosby Galvan: laboratory work. Revised research proposal and final manuscript.

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