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Revista mexicana de ciencias forestales

versión impresa ISSN 2007-1132

Rev. mex. de cienc. forestales vol.11 no.62 México nov./dic. 2020  Epub 19-Feb-2021

https://doi.org/10.29298/rmcf.v11i62.809 

Research notes

Seedling quality from six Pinus species produced in polyethylene bag

Tomás Pineda Ojeda1 

Eulogio Flores Ayala1 

Andrés Flores2  * 

Enrique Buendía Rodríguez1 

Vidal Guerra de la Cruz3 

Fabián Islas Gutiérrez1 

1Campo Experimental Valle de México, CIR-Centro, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. México.

2Centro Nacional de Investigación Disciplinaria en Conservación y Mejoramiento de Ecosistemas Forestales, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. México.

3Sitio Experimental Tlaxcala, CIR-Centro, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. México.


Abstract

The restorers of degraded areas require high quality plants, with ideal morphological attributes to be able to establish successfully in the plantation sites, even if the site conditions are adverse. In order to know the plant quality of six pine species that will be used for reforestation and restoration of forest lands in the state of México, five morphological attributes based on the Mexican Norm NMX-AA-170-SCFI-2016 and a paper were assessed. At the Héroes Bicentenario Forest Nursery of Tecámac, seedling’s basal diameter (at the root neck (DC)), height, from the base to the apical bud (Alt), slenderness index (IE = Alt/DC), aerial dry biomass and dry root biomass ratio (BSA/BSR) and Dickson's quality index (ICD) were determined. For DC, the results showed that all species except for Pinus ayacahuite, had high quality. In Alt, P. greggii and P. leiophylla had the best quality; while in IE, all the species except for P. greggii, showed high quality. For the BSA/BSR ratio, P. cembroides, P. ayacahuite and P. hartwegii were the best, and for ICD, P. hartwegii had the highest quality value. Considering all the morphological indicators together, it was concluded that P. cembroides and P. greggii had the best plant quality. This information is crucial to analyze the seedling’s survival in the field and make timely decisions in land restoration management.

Key words Morphological attributes; increased survival; Probosque; nursery plant production; reforestation; forest restoration

Resumen

Los restauradores de áreas degradadas requieren de planta de calidad con atributos morfológicos ideales para lograr su establecimiento con éxito en el sitio de la plantación, aun cuando las condiciones del sitio sean adversas. Para conocer la calidad de seis especies de pino que serán empleadas durante la reforestación y restauración de terrenos forestales en el Estado de México, se realizó la evaluación de cinco atributos morfológicos con base en lo citado en la Norma Mexicana NMX-AA-170-SCFI-2016 y un artículo científico en el Vivero Forestal Héroes Bicentenario de Tecámac: diámetro al cuello de la raíz (DC), altura desde la base hasta la yema apical (Alt), índice de esbeltez (IE=Alt/DC), relación biomasa seca aérea y biomasa seca de raíz (BSA/BSR) e índice de calidad de Dickson (ICD). Para DC, los resultados mostraron que todas las especies, excepto Pinus ayacahuite, tuvieron calidad alta. En Alt, P. greggii y P. leiophylla tuvieron la mejor calidad; mientras que en IE, todos los taxones, menos P. greggii, presentaron alta calidad. Para la relación BSA/BSR: P. cembroides, P. ayacahuite y P. hartwegii fueron los mejores, y para ICD, P. hartwegii presentó el mayor valor de calidad. Al considerar todos los indicadores morfológicos juntos, se determinó que P. cembroides y P. greggii registraron la mejor calidad de planta. Esta información es clave para analizar la supervivencia esperada en campo y tomar decisiones oportunas en la gestión forestal.

Palabras clave Atributos morfológicos; incremento de supervivencia; Probosque; producción de planta en vivero; reforestación; restauración forestal

During the reforestation process in degraded areas, it is necessary to use quality plants so that they have a greater ability to establish, grow and develop in the plantation sites (Muñoz et al., 2015). Consequently, its use increases the survival percentage, since, once established in the field, it will be able to live and grow, even in places with low productivity.

However, it is difficult to obtain suitable plant for each site. The attributes that determine its quality refer to morphological and physiological qualities (Mexal and Landis, 1990). The former are related to the structure of the plant; while, the physiological ones to the responses it has when being planted (Rose et al., 1990). The morphological criteria are more often used than the physiological ones to assess plant quality (Haase, 2008).

The main morphological attributes are stem height, neck diameter, root system, height-diameter ratio (robustness index), biomass, foliar area and presence of mycorrhizae (Escobar-Alonso and Rodríguez, 2019). In regard to the physiological ones, those used are the concentration of carbohydrates, nutritional status, water potential, electrical conductivity, stomatal conductivity, CO2 assimilation and chlorophyll fluorescence (Escobar-Alonso and Rodríguez, 2019).

Despite the fact that there is experience on the evaluation of the plant quality parameters in Mexican forest species, the great variability in conditions and production processes in the nurseries means that knowledge in this sense is still limited, so it is necessary to carry out more research in this regard. Therefore, the present work aimed to assess the quality of the plant in the nursery of six species of the Pinus genus produced in polyethylene bags.

At the Héroes Bicentenario Forest Nursery located in Tecámac, State of Mexico (administered by the Protectora de Bosques of the State of Mexico), 90 Pinus plants of six species were randomly chosen (15 plants per species), produced in natural ground and polyethylene bags (10 × 24 cm). The age of the plant material was different: Pinus greggii Engelm. ex Parl. and P. leiophylla Schiede ex Schltdl. & Cham., were 7 months-old; P. pseudostrobus Lindl., 10 months-old; P. ayacahuite Ehrenb. ex Schltdl., 12 months-old; P. hartwegii Lindl., 14 months-old and P. cembroides Zucc., 15 months-old.

Each individual was evaluated for their plant quality by measuring the diameter (mm) at the root neck (DC) with a Mitutoyo 500 - 196 - 30 digital vernier, and the height (cm) from the base to the apical bud (Alt) with a Westcott H-6560 graduated ruler; the data of both variables were used to calculate the Slenderness Index (IE = Alt / DC). Substrate was removed from all plants and the stem and root were sectioned.

The plant material (aerial part and root) was placed separately in paper bags, dried in a Novatech HS35-AIA oven (50 °C, 9 days) and weighed with a Ohaus, Harvard Trip® two-plates scale to estimate aerial dry biomass (BSA, g), root dry biomass (BSR, g), total dry biomass (BST, g), BSA / BSR ratio and Dickson's quality index [ICD = BST / (IE + BSA / BSR)] (Dickson et al., 1960).

To determine the quality of the plants (high, medium or low), the values obtained for DC and Alt were compared to the morphological indicators of plant quality at the nursery that are stipulated in the NMX-AA-170-SCFI-2016 Mexican Standard (Secretaría de Economía, 2016); while IE, BSA / BSR and ICD with the intervals cited by Rodríguez-Ortiz et al. (2020) (Table 1).

Table 1 Values and intervals of morphological indicators of plant quality. 

Variable / Species Plant quality
B = Low M = Medium A = High
DC (mm)
Pinus ayacahuite Ehrenb. ex Schltdl. - - ≥ 4
Pinus cembroides Zucc. - - ≥ 4
Pinus greggii Engelm. ex Parl. - - ≥ 4
Pinus hartwegii Lindl. - - ≥ 4
Pinus leiophylla Schiede ex Schltdl. & Cham. - - ≥ 4
Pinus pseudostrobus Lindl. - - ≥ 4
Alt (cm)
Pinus ayacahuite Ehrenb. ex Schltdl. 20 - 30
Pinus cembroides Zucc. 15 - 25
Pinus greggii Engelm. ex Parl. 25 - 30
Pinus hartwegii Lindl. N/A N/A N/A
Pinus leiophylla Schiede ex Schltdl. & Cham. 20 - 25
Pinus pseudostrobus Lindl. 25 - 30
IE ≥ 8.0 (8.0 a 6.0) < 6.0
BSA/BSR > 2.5 (2.0 a 2.5) < 2.0
ICD < 0.2 (0.2 a 0.5) > 0.5

B = Plants with two or more B quality values, plants that will have low survival and small development in the plantation sites; M = Accepts up to three M quality values and one variable with B quality qualification; A = Plants with minimal presence of undesirable characteristics, and that can accept up to two values with quality M, but in no case values with quality B (Rodríguez-Ortiz et al., 2020); † = NMX-AA-170-SCFI-2016 Mexican Standard (Secretaría de Economía, 2016); ‡ = Quality intervals (Rodríguez-Ortiz et al., 2020).

In regard to DC, all species, except for P. ayacahuite, recorded higher values than those indicated in the NMX-AA-170-SCFI-2016 (Secretaría de Economía, 2016), and thus, they were considered high quality; P. hartwegii in particular, was the best of all, since it more than doubled the value indicated in the Standard (Table 2).

Table 2 Average values of plant sizes and qualities of six species of pine produced in the Héroes Bicentenario Forest Nursery. 

Species DC Alt IE BSA/BSR ICD
Pinus ayacahuite Ehrenb. ex Schltdl. 3.7 16.2 4.4 1.9 0.9
Pinus cembroides Zucc. 4.6 15.9 3.5 1.5 1.1
Pinus greggii Engelm. ex Parl. 5.0 36.5 7.5 4.6 0.7
Pinus hartwegii Lindl. 9.2 18.4 2.0 2.6 2.2
Pinus leiophylla Schiede ex Schltdl. & Cham. - 28.4 3.8 5.0 0.9
Pinus pseudostrobus Lindl. 4.8 20.7 4.4 8.3 0.5

DC = Neck diameter (mm); Alt = Height (cm); IE = Slenderness index; BSA = Aerial dry biomass; BSR = Root dry biomass; ICD = Dickson's Quality Index.

Results suggested that five species of pine evaluated had the potential to establish themselves in the field, since the diameter dimensions indicate well-developed root systems, resistance to physical damage, twists, tolerance to those caused by animals and to high temperatures on the soil surface (Mexal and Landis, 1990; Birchler et al., 1998; Muñoz et al., 2015).

When comparing the production of these pine trees with that registered in other nurseries, its superiority is evident; for example, in P. greggii and P. pseudostrobus a medium quality is reported [3.5 and 3.4 mm, respectively] (Rueda et al., 2012).

In regard to Alt, P. greggii and P. leiophylla were above the value suggested in the NMX-AA-170-SCFI-2016 Mexican Standard and P. cembroides had values within the proposed interval; however, P. ayacahuite and P. pseudostrobus had lower height than advisable (Table 2). For P. hartwegii, the NMX-AA-170-SCFI-2016 Mexican Standard does not include a maximum or a minimum value, as it is a cespitose species; however, the record obtained was considerably higher than that documented by Bernaola-Paucar et al. (2015) (4.3 cm) in 12-month-old trees.

In general, height in pines is a variable that has little value as an indicator of quality, and it does not even correlate, or it does so negatively, with survival in the field (Birchler et al., 1998). However, this characteristic combined with other variables, such as the diameter of the neck (Slenderness Index), acquires greater importance.

For IE, all the species, except for P. greggii, recorded a value lower than six (Table 2), which indicates that the plants are more robust, of better quality, that can resist abuse from transfer maneuvers to the plantation site, to drying out by the wind, frost damage and with a better chance of survival and growth in sites with water limitations (Haase, 2008; Escobar-Alonso and Rodríguez, 2019). In the case of P. greggii, excessive growth in height (36.5 cm) was observed, despite the fact that its diameter corresponded to the NMX-AA-170-SCFI-2016 Mexican Standard (> 4 cm); this ratio would improve with a lower density in the growth bed during the production phase at the nursery, or with pruning to the aerial part prior to going to the field (Muñoz et al., 2015).

In the BSA / BSR ratio, P. cembroides, P. ayacahuite and P. hartwegii recorded the lowest proportions (Table 2) which indicated high quality, from a good balance between the biomass of the aerial part and the root (Haase, 2008), as well as greater possibilities of success when planted in places with low rainfall (Thompson, 1985).

Conversely, P. pseudostrobus, P. leiophylla and P. greggii had the highest ratios (Table 2), which suggested that they should be planted in places where there are no water availability problems; from its poorly developed root structure, its ability to absorb nutrients and water will be limited to supply the needs of the aerial part (or of the plant) (Escobar-Alonso and Rodríguez, 2019). This can lead to stress due to lack of moisture in dry places before the roots are established, and with this occurs the supply of water and nutrients to the aerial part of the plant.

For ICD, P. hartwegii showed the highest value; while P. greggii and P. pseudostrobus had the lowest; P. cembroides, P. ayacahuite and P. leiophylla had intermediate values. It was determined that all species, except for P. pseudostrobus, show high quality, since their ICD was > 0.5 (Rodríguez-Ortiz et al., 2020).

When combining the values of the different evaluated, variables four species, P. hartwegii, P. cembroides, P. leiophylla and P. ayacahuite registered the best plant quality, with which it can be predicted that they will have greater development possibilities, even if they are established in sites whose conditions are unfavorable for them.

Part of the success of reforesting degraded areas lies in the use of quality plants, with morphological characteristics that allow them to survive and grow, even in unsuitable sites. However, the use of reference values, as well as the evaluation of the morphological attributes of the species produced in the nursery, are still limited in the country (Rueda et al., 2014). The results of the present work showed that P. cembroides and P. greggii have high plant quality; P. ayacahuite, low quality; P. hartwegii, P. leiophylla and P. pseudostrobus, medium quality. This information is crucial to analyze the survival that will be obtained in the field, depending on the characteristics of each site, and will favor timely decisions to be made in the forest management of the reforestation areas in which the plant production lots of the plants of the assessed species are intended.

Acknowledgements

The authors thank INIFAP for sponsoring this work through the budgetary project Number 2-1.6-1175934783-FM.2-1 “Increase of survival and initial growth in forest plantations for recovery purposes, through the application of antiperspirants, in species of the Pinus genus”, and to the Protectora de Bosques of the State of Mexico (PROBOSQUE) for having provided the plants of the six species to carry out the plant quality evaluation.

REFERENCES

Bernaola-Paucar, R. M., E. Pimienta B., P. Gutiérrez G., V. M. Ordaz C., G. Alejo S., G. y E. Salcedo P. 2015. Efecto del volumen del contenedor en la calidad y supervivencia de Pinus hartwegii Lindl. en sistema doble-trasplante. Revista Mexicana de Ciencias Forestales 6(28): 174-187. Doi: 10.29298/rmcf.v6i28.275. [ Links ]

Birchler, T., R. W. Rose, A. Royo y M. Pardos. 1998. La planta ideal: revisión del concepto, parámetros definitorios e implementación práctica. Investigación Agraria. Sistemas y Recursos Forestales, 7(1): 109-121. https://recyt.fecyt.es/index.php/IA/article/view/2806 (17 de junio de 2020). [ Links ]

Dickson A., A. L. Leaf and J. F. Hosner. 1960. Quality appraisal of white spruce and white pine seedling in nurseries. The Forestry Chronicle 36(1): 10-13. http://pubs.cif.ifc.org/doi/pdf/10.5558/tfc36010-1 (17 de junio de 2020). [ Links ]

Escobar-Alonso, S. y D. A. Rodríguez T. 2019. Estado del arte en la investigación sobre calidad de planta del género Pinus en México. Revista Mexicana de Ciencias Forestales 10(55): 4-38. Doi: 10.29298/rmcf.v10i55.558. [ Links ]

Haase, D. L. 2008. Understanding forest seedlings quality: Measurements and interpretation. Tree Planter´s Notes, 52 (2): 24-30. http://forestseedlingnetwork.com/media/15912/Understanding_Forest_Seedling_Quality_-_Measurements_and_Interpretation.pdf (17 de junio de 2020). [ Links ]

Mexal, J. G. and T. D. Landis. 1990. Target seedling concepts: height and diameter. In: Rose, R., S. J. Campbell and T. D. Landis (eds.). Target seedling symposium: proceedings, combined meeting of the western forest nursery associations. U.S. Department of Agriculture, Forest Service. Fort Collins, CO, USA. pp. 17-35. [ Links ]

Muñoz F., H. J., J. T. Sáenz R., V. M. Coria A., J. de J. García M., J. Hernández R. y G. E. Manzanilla Q. 2015. Calidad de planta en el vivero forestal La Dieta, Municipio Zitácuro, Michoacán. Revista Mexicana de Ciencias Forestales , 6(27): 72-89. Doi: 10.29298/rmcf.v6i27.282. [ Links ]

Rodríguez-Ortiz, G., R. D. Aragón-Peralta, J. R. Enríquez-del Valle, A. Hernández-Hernández, W. Santiago-García y G. V. Campos-Angeles. 2020. Calidad de plántula de progenies selectas de Pinus pseudostrobus Lindl. var. oaxacana del sur de México. Interciencia 45(2): 96-101. https://www.researchgate.net/publication/339750861_Calidad_de_plantula_de_progenies_selectas_de_Pinus_pseudostrobus_Lindl_var_oaxacana_del_sur_de_Mexico (17 de junio de 2020). [ Links ]

Rose, R., C. William and P. Morgan. 1990. The target seedling concept. In: Rose R., S. J. Campbell and T. D. Landis (eds.). Target seedling symposium: proceedings, combined meeting of the western forest nursery associations. U.S. Department of Agriculture, Forest Service. Fort Collins, CO, USA pp. 1-8. [ Links ]

Rueda S., A., J. de D. Benavides S., J. Á. Prieto R., J. T. Sáenz R., G. Orozco G. y A. Molina C. 2012. Calidad de planta producida en los viveros forestales de Jalisco. Revista Mexicana de Ciencias Forestales 3(14): 69-82. Doi: 10.29298/rmcf.v5i22.350. [ Links ]

Rueda S., A., J. de D. Benavides S., T. Sáenz R., H. J. Muñoz F., J. Á. Prieto R. y G. Orozco G. 2014. Calidad de planta producida en los viveros forestales de Nayarit. Revista Mexicana de Ciencias Forestales 5(22): 58-72. Doi: 10.29298/rmcf.v5i22.350. [ Links ]

Secretaría de Economía. 2016. Norma Mexicana NMX-AA-170-SCFI-2016. Certificación de la operación de viveros forestales. http://sivicoff.cnf.gob.mx/ContenidoPublico/10%20Material%20de%20Consulta/Normatividad%20Vigente/NMX-AA-170-SCFI-2016%20viveros%20forestales.pdf (13 de junio de 2020). [ Links ]

Thompson, B. E. 1985. Seedling morphological evaluation. What can you tell by looking? In: Duryea, M. L. (Ed.). Evaluating seedling quality: principles, procedures and predictive abilities of major tests. Oregon State University. Corvallis, OR, USA. pp. 59-71. [ Links ]

Received: June 27, 2020; Accepted: September 30, 2020

Conflict of interests

The authors declare no conflict of interests.

Contribution by author

Tomás Pineda Ojeda: planning of the investigation, supervision of works, structuring and writing of the manuscript; Eulogio Flores Ayala: evaluation of variables, writing and discussion of the manuscript; Andrés Flores: data analysis and writing of the manuscript; Enrique Buendía Rodríguez: work planning and evaluation of variables; Vidal Guerra de la Cruz: supervision of works, evaluation of variables; Fabián Islas Gutiérrez: evaluation of variables.

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