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Revista mexicana de ciencias agrícolas
versión impresa ISSN 2007-0934
Rev. Mex. Cienc. Agríc vol.10 no.2 Texcoco feb./mar. 2019
https://doi.org/10.29312/remexca.v10i2.1594
Articles
Incidence and severity of the common blight in bean plants inoculated with Rhizobium phaseoli
1Departamento de Parasitología Agrícola-Universidad Autónoma Agraria Antonio Narro. Buenavista, Saltillo, Coahuila, México. CP. 25315. Tel. 01(844) 4110326. (uaaan-osvaldo@hotmail.com; fdanielhc@hotmail.com; melchoresraza2010@hotmail.com; aspeytia.david@inifap.gob.mx).
2Campo Experimental Saltillo-INIFAP. Carretera Saltillo-Zacatecas km 342+119, núm. 9515, Colonia Hacienda de Buenavista, Saltillo, Coahuila, México. CP. 25315. Tel. 01(844) 2912234.
The common bacterial blight Xanthomonas axonopodis pv. phaseoli is a disease that attacks the bean crop (Phaseolus vulgaris), is present 83% of the areas of seed production and up to 79% in commercial fields and reduces yields up to 55%. The inoculation of Rhizobium in plants as a promoter of plant growth, nitrogen fixer, to the synthesis of indoleacetic acid, produces in the plant greater vigor and tolerance to diseases. The behavior of the incidence and severity of common blight of bean plants inoculated with Rhizobium phaseoli and X. axonopodis pv. phaseoli. For this, strains of Rhizobium (FM-1, N-2, PS-3, M-4, BJ-5) of root nodules of bean plants and Xanthomonas (Xant 1) of necrotic leaf spots were isolated. The isolates were identified based on cellular, colonial and biochemical morphological characteristics. The greenhouse and field trials were carried out on seeds inoculated with 106 cfu mL-1 of Rhizobium and a second application 10 days after planting. Subsequently, 15 days after emergence of the bean, Xanthomonas axonopodis was inoculated directly by sprinkling directly into the plant. After 30 days of greenhouse cultivation, it was observed that the severity of common blight was lower in plants with nodules in the root (14.5%), than in the control (46%), it was also found that plants inoculated with Rhizobium phaseoli BJ -5 developed greater vigor, stem diameter, dry weight, number of leaves and root length. In the field, the inoculation with strain BJ-5 also showed lower severity (25.6%) compared to the control (55.8%) and it turned out to have better growth in the plant. The inoculation of beans with Rhizobium phaseoli allows the crop to be nutritionally and phytosanitarily favored.
Keywords: biological control; phytosanitary; promoter of growth
El tizón común bacteriano Xanthomonas axonopodis pv. phaseoli es una enfermedad que ataca al cultivo de frijol (Phaseolus vulgaris), se encuentra presente 83% de las áreas de producción de semilla y hasta 79% en campos comerciales y reduce los rendimientos hasta 55%. La inoculación de Rhizobium en plantas como promotor de crecimiento vegetal, fijador de nitrógeno, a la síntesis del ácido indolacético, producen en la planta mayor vigor y tolerancia a las enfermedades. Se determinó el comportamiento de la incidencia y severidad del tizón común de plantas de frijol inoculadas con Rhizobium phaseoli y X. axonopodis pv. phaseoli. Para ello se aislaron cepas de Rhizobium (FM-1, N-2, PS-3, M-4, BJ-5) de nódulos radiculares de plantas de frijol y Xanthomonas (Xant 1) de manchas foliares necróticas. Los aislados se identificaron con base en características morfológicas celulares, coloniales y bioquímicas. Los ensayos en invernadero y en campo, se efectuaron en semillas inoculadas con 106 ufc mL-1 de Rhizobium y una segunda aplicación a los 10 días después de la siembra. Posteriormente 15 días después de la emergencia del frijol se inoculó por aspersión Xanthomonas axonopodis directamente a la planta. Transcurridos 30 días del cultivo en invernadero se observó que la severidad del tizón común fue inferior en plantas con presencia de nódulos en raíz (14.5%), que en el testigo (46%), también se encontró que las plantas inoculadas con Rhizobium phaseoli BJ-5 desarrollaron mayor vigor, diámetro de tallo, peso seco, número de hojas y longitud de raíz. En campo, la inoculación con la cepa BJ-5 mostró menor severidad (25.6%) respecto al testigo (55.8%) y resultó tener mejor crecimiento en la planta. La inoculación de frijol con Rhizobium phaseoli permite favorecer nutricional y fitosanitariamente al cultivo.
Palabras claves: control biológico; fitosanitario; promotor de crecimiento
Introduction
The common bacterial blight Xanthomonas axonopodis pv. phaseoli is a bacterium that attacks the cultivation of bean (Phaseolus vulgaris) worldwide (Gent et al., 2005). It is present in 83% of the areas of seed production and up to 79% in commercial fields, it reduces yields by 55%, those that increase at temperatures of 27 °C, with high relative humidity (Fourie, 2002; Belete and Basta, 2017). For its management, chemical, biological, cultural and genetic control methods can be used (Francisco et al., 2013). The use of chemicals has allowed for greater production, but excessive use causes problems to the environment, so searches for alternatives to the management of diseases are of vital importance (Zavaleta, 2000). Compatibility has been reported in Pseudomonas sp., Bacillus cereus and Rhodococcus fascians with Rhizobium leguminosarum bv. phaseoli, whose symbiosis helps develop a protective activity against common bean blight (Zanatta et al., 2007).
The plant growth promoting rhizobacteria (RPCV), are used as biological control agents, these bacteria are characterized by their ability to directly or indirectly facilitate the development of the root and foliage, the indirect stimulation of plant growth is due to the fact that the bacterium allows fungal action (Essalmani and Lahlou, 2003). Direct stimulation may include nitrogen fixation (Sessitsch et al., 2002), the production of hormones (Perrine et al., 2004), enzymes (Mayak et al., 2004) and the solubilization of phosphates (Rodríguez and Fraga, 1999). The objective of this work was to determine the behavior of bean plants, inoculated with Rhizobium phaseoli and X. axonopodis pv. phaseoli and its relationship with the presence and development of common blight in the plant.
Materials and methods
Isolation of Xanthomonas spp.
Plants with irregular necrotic leaf spots surrounded by yellow halo were collected in different areas of the leaves (Fourie, 2002) in the experimental field The Bajío of the Autonomous Agrarian University Antonio Narro (UAAAN). The samples were cut into pieces, disinfected with 1% hypochlorite for 3 min and washed with sterile distilled water, then placed in a sterile mortar where they were macerated in 0.85% saline, then a roast was taken, which was fluted. in YDC culture medium (Schaad, 1988). The plates were incubated at 28 °C for 48 h. At the end of the period, bacterial colonies were selected with yellow color, convex, with whole borders and mucoid appearance (Schaad, 2001) and purified by stria. The identification was made by morphological, colonial and biochemical characterization (Abd-Alla and Bashandy, 2008; Francisco et al., 2013; Osdaghi, 2014).
Isolation of Rhizobium spp.
Samples of reddish colored nodules of different bean varieties were collected in the prefloration period (Table 1), washed and disinfected with 1% hypochlorite for 3 min, then washed three times with sterile distilled water and placed in a sterile tube. where they macerated, a roast was later taken and plated with the yeast mannitol agar red congo extract (ELMA-RC) (Vincent, 1970; CIAT, 1988). Plates were incubated at 28 °C for 4 days. The red colonies were reseeded in plates with the same medium, until obtaining pure cultures, each isolate was characterized by physiological, biochemical, morphological and colonial tests.
Table 1 Location coordinates of the study area.
| Location | Coordinates | Altitude (m) | Samples | Strains |
| UAAAN Buenavista, Saltillo, Coahuila. | 25° 22'' North and 101° 02'' West | 1 742 | Bean, pre-flowering stage | FM-1 |
| San Andres Tlalamac, Edo. México. | 18° 58'' 1'' North and 98° 48'' 28'' West | 2 073 | Bean, pre-flowering stage | N-2 and M-4 |
| UAAAN Buenavista, Saltillo, Coahuila. | 25° 22'' North and 101° 02'' West | 1 742 | Bean, pre-flowering stage | PS-3 and BJ-5 |
Bacterial reproduction
To increase the populations of Xanthomonas liquid YDC was used, while for Rhizobium it was liquid ELMA, propagating in a rotary incubator (C25 Incubator Shaker New Brunswick Scientific) at 28 °C and 150 rpm for 72 h, to obtain each bacterium a cell concentrate
Greenhouse experiment
To determine the relationship between the presence of common blight and its severity in bean plants inoculated with R. phaseoli, bean seeds were initially disinfected, for which 1% sodium hypochlorite was used for one minute, washing the seeds three times with sterile water. Each seed was impregnated by spraying with a solution of 1 x 106 cells of R. phaseoli, later they were placed in unicel containers of 1 liter capacity with – parts of peat moss-pearlite (in proportion 3:1), a second application it was done 10 days after sowing. Each plant represented an experimental unit, being ten plants per treatment (Table 2).
Table 2 Experimental design to evaluate the behavior of Phaseolus vulgaris to the inoculation of R. phaseoli and common blight.
| Treatments | Strains | |||||
| FM-1 | N-2 | PS-3 | M-4 | BJ-5 | Xant-1 | |
| I. Rhizobium (FM-1) | + | - | - | - | - | + |
| II. Rhizobium (N-2) | - | + | - | - | - | + |
| III. Rhizobium (PS-3) | - | - | + | - | - | + |
| IV. Rhizobium (M-4) | - | - | - | + | - | + |
| V. Rhizobium (BJ-5) | - | - | - | - | + | + |
| VI. Irrigated only with uninoculated water, absolute control(CA) | - | - | - | - | - | + |
+= applied; -= not applied.
The pathogen (Xanthomonas axonopodis) was inoculated to fifteen days of growth of the culture, with a suspension of 1.6 x 106 cells, applied by spray to the foliage (Pastor, 1991; Abeysinghe, 2009). The evaluation was made thirty days later to determine the incidence and severity of common blight. according to the scale of the degree of yellowness and necrosis of the inoculated plants: 0= absence of symptoms; 1= from 1 to 12.5% of damaged area; 2= from 13 to 25.5%; 3 from 26 to 38.5%; 4= from 39 to 51.5%; 5= from 52 to 64.5%; 6= from 65 to 77.5%; 7= from 78 to 90.5%; 8= from 91 to 100% of damaged leaf area (Gilberston et al.,1988; Osdaghi et al., 2009).
Field experiment
To determine the behavior of bean plants inoculated with R. phaseoli and the presence of common blight in the field, an experimental plot of 10 m long with five furrows at 80 cm distance between them was established. Each Rhizobium isolate was applied as a treatment (Table 2), to inoculate the seed and the plant the same procedure was followed for the greenhouse experiment.
Subsequently, the inoculated seeds were sown at a depth of 3 cm interspersed every 10 cm in the furrow in the whole plot established with a drip irrigation system. Fifteen days after sowing Xanthomonas axonopodis was applied at a concentration of 1.6 x 106 cfu mL-1 to the foliage of the crop (Pastor, 1991) and 50 days later the incidence and severity of the common blight with the scale was determined (Gilberston et al., 1988; Osdaghi et al., 2009).
Statistical analysis
The behavior of the variables analyzed in each experiment, as well as the incidence and severity of common bean blight in each plant, was processed under a completely randomized design, where each plant was considered as an experimental unit, taking data from 10 plants by treatment. The variance between the data (Anova) was analyzed through means comparison according to Tukey (˂0.05) processing the data in the statistical package SAS Statistical Analysis System version 9.0.
Results and discussion
Five isolates of Rhizobium spp. were recovered from radicular nodules of bean plants, in the Table 3 shows the results of different biochemical tests and the characteristics of shape, edge, elevation, appearance and color, which indicate that the isolates have the characteristics of a good inoculant of the Rhizobium phaseoli type, which agrees with studies carried out by (Cuadrado et al., 2009; Berrada et al., 2012; Granda et al., 2013).
Table 3 Biochemical, morphological and colonial behaviors of R. phaseoli.
| Strains | Biochemical and morphological | Colonial | ||||||||||
| Gram stain | Oxidase | Catalase | Growth in NaCl 2, 3 and 5% | Growth in pH 4, 5, 7 and 9 | MacConckey growth | Growth AML-ABT | Shape | Edge | Elevation | Appearance | Colour | |
| FM-1 | - | - | - | + | + | + | + | Circulate | Wavy | Convex | Mucoid | Red |
| N-2 | - | - | + | + | + | + | + | Circulate | Wavy | Convex | Mucoid | Red |
| PS-3 | - | - | + | + | + | + | + | Circulate | Wavy | Planoconvex | Mucoid | Red |
| M-4 | - | - | + | + | + | + | + | Circulate | Wavy | Planoconvex | Mucoid | Red |
| BJ-5 | - | - | + | + | + | + | + | Circulate | Wavy | Convex | Mucoid | Red |
Coloration of the colonies of the isolates obtained with a beige color at 72 h, which becomes intense red after 8 days of incubation (Rojas et al., 2009) to rule out similarity, biochemical tests were performed with the genus Agrobacterium, the medium mannitol yeast added bromothymol blue (LMA-ABT) was used, where the Rhizobium isolates acidified the medium turning it yellow while the reports indicate that Agrobacterium alkalizes the medium turning it blue (Cuadrado et al., 2009).
From leaves with symptoms of common blight, strains were identified that presented characteristics typical of the genus Xanthomonas, such as yellow colonies with a whole, convex edge and a mucoid appearance, which agrees with what was described by (Corzo et al., 2015). Isolated strains were negative for gram stain, levana production and fluorescent pigments, while peroxidase, catalase, oxidative and fermentative metabolism of glucose and starch hydrolysis tests were positive, indicating that the recovered strain corresponds to Xanthomonas axonopodis and agrees with the characteristics reported by (Schaad, 2001; Francisco et al., 2013).
Greenhouse experiment
The inoculation of Rhizobium phaseoli in bean plants induced a greater tolerance to leaf expression of common blight. In Figure 1, it is shown that the strain Rhizobium phaseoli-BJ-5 showed lower severity (14.5%) to this disease compared to the absolute control inoculated with Xanthomonas axonopodis that registered 46% damage.
Figure 1 Effect of the inoculation of Rhizobium phaseoli on Phaseolus vulgaris and the presence and development of common blight in the greenhouse. T1= Rhizobium (FM); T2= Rhizobium (N); T3= Rhizobium (PS); T4=Rhizobium (M); T5=Rhizobium (BJ) and T6= absolute control (CA).
It was also observed that all the treatments behaved statistically equal (p< 0.05) with respect to the incidence of common blight with respect to the control, which indicated that the pathogen is active in all infested plants and there is no difference between them regarding their presence, but not in the degree of response or damage in the manifestation of the disease where two of the inoculants of Rhizobium phaseoli T2 and T5, induced or reduced the level of damage (Figure 1) of the common blight with respect to the control statistically (p< 0.05), similar experimental results were reported by Zanatta et al. (2007) who reports the induction of resistance generated by Rhizobium spp. in bean plants.
While Osdagui et al. (2011) report results similar to those of the present study, where they show significant differences in the manifestation of common blight in plants inoculated with Rhizobium leguminosarum pv phaseoli compared with plants fertilized with urea and the control.
Table 4 shows the response of Phaseolus vulgaris infested with Xanthomonas axonopodis and with five inoculants of R. phaseoli; through vegetable biomass produced, where it is observed that in most of the quantified variables the inoculated plants showed greater weight, number of leaves and root length than the uninoculated control, coupled with higher nodulation by rhizobia when using them as inoculants favored a greater number and the presence of nodules in the plants within the experiment.
Table 4 Behavior of agronomic variables in bean plants inoculated with Rhizobium phaseoli and Xanthomonas axonopodis in greenhouse.
| Treatments | Fresh weight (g) | Dry weight (g) | No. of leaf | Root length (cm) | Stem diameter (mm) | No. of nodules |
| Rhizobium FM-1 | 35.05 a | 5.6 a | 31.8 ab | 20.6 abc | 4.06 b | 199 a |
| Rhizobium N-2 | 25.75 ab | 4.2 a | 26.8 c | 21.6 a | 4.32 b | 120.2 b |
| Rhizobium PS-3 | 25.34 ab | 4.4 a | 27.6 bc | 21.2 ab | 5.22 a | 116 b |
| Rhizobium M-4 | 25.25 ab | 3.6 a | 28 abc | 19.2 bc | 4.08 b | 73 bc |
| Rhizobium BJ-5 | 33.09 a | 5.6 a | 32.6 a | 21 abc | 4.44 b | 228 a |
| Control absoluto | 16.82 b | 3.6 a | 29 abc | 18.8 c | 4.08 b | 48 c |
| SE ± | 1.38 | 0.25 | 0.57 | 0.27 | 0.09 | 12.61 |
| CV | 18.78 | 27.51 | 8.41 | 5.73 | 7.73 | 19.36 |
Values with different letters have statistical differences according to Tukey ˂ 0.05.
The strains of Rhizobium spp., used in this study induced the improvement to the crop possibly due to the ability of the rhizobia to produce hormones such as indole acetic acid and gibberellic acid growth regulating substances in the plants, observing an increase in emergence, vigor, biomass and development of the root system (Dey et al., 2004; Perrine et al., 2004; Lugtenberg and Kamilova, 2009). Another study showed that Phaseolus vulgaris inoculated with Rhizobium etli had 100% germination or emergence in 4 and 8 days, respectively, values with statistical difference compared to 62.5% in 7.5 days with Phaseolus vulgaris without inoculation and as absolute control (García et al., 2016).
Field experiment
As shown in Figure 2, the presence of X. axonopodis in the leaves of the bean plant clearly decreased by the inoculation of Rhizobium phaseoli strain BJ-5 (18.4%) followed by strain FM-1 (19.8%) with with respect to absolute control (CA).
Figure 2 Effect of the inoculation of Rhizobium phaseoli on Phaseolus vulgaris and the presence and development of common blight in the field. T1= Rhizobium (FM); T2= Rhizobium (N); T3= Rhizobium (PS); T4= Rhizobium (M); T5= Rhizobium (BJ) and T6= absolute control (CA).
The severity of the disease in the foliar leaves of the bean plant in the crop was classified as mild, given that the strain BJ-5 (25.6%) the numerical values in this phase, were statistically different compared to the non-inoculated or CA with 55.8%, the effect shown by the inoculation of R. phaseoli in the bean crop in the presence and severity of the disease in the leaves, emphasizing the decrease in strain BJ-5.
Similar results have been reported by Osdagui et al. (2011). Those who report that the severity of the disease in the field evaluated at 50 days was very severe (complete necrosis of the leaves) in the susceptible cultivar and line that were fertilized with urea and in the control group, with very severe loss in the yield, but in the plants inoculated with R. leguminosarum pv phaseoli, the severity of the disease was relatively low (p< 0.01). According to Bartsev et al. (2004) Rhizobium spp., can induce resistance in common beans and limit the presence of bacterial common blight. Other studies mention that the treatment of Rhizobium spp., reduces stem and root rot compared to non-inoculated plants (Becquer, 2011; Rahman et al., 2017).
Table 5 presents the response of P. vulgaris in the yield of biomass at the Rhizobium seedling level in the field where, like the greenhouse higher yield of fresh, dry weight, number of leaves, stem diameter and root length was observed in inoculated plants, than those not inoculated, highlighting the strain Rhizobium phaseoli BJ-5 where these variables had higher values in yield.
Table 5 Behavior of agronomic variables in plants of Phaseolus vulgaris inoculated with Rhizobium phaseoli and Xanthomonas axonopodis in the field.
| Treatments | Fresh weight (g) | No. of leaves | Root length (cm) | Stem diameter (mm) | No. of nodules |
| Rhizobium FM-1 | 404 a | 68.2 a | 17.4 ab | 8.72 a | 54.2 ab |
| Rhizobium N-2 | 290 ab | 61.4 ab | 18.68 ab | 9.8 a | 34 b |
| Rhizobium PS-3 | 390 a | 63 ab | 17.62 ab | 8.76 a | 34.2 b |
| Rhizobium M-4 | 312 ab | 51 ab | 15.34 b | 7.9 a | 39.2 ab |
| Rhizobium BJ5 | 535 a | 69.8 a | 19.98 ab | 9.78 a | 79.8 a |
| Control absoluto | 136 b | 43.8 b | 20.2a | 8.34 a | 12 b |
| SE ± | 31.21 | 2.39 | 0.5 | 0.22 | 5.3 |
| CV | 37.51 | 16.87 | 13.26 | 12.77 | 51.78 |
Values with different letters have statistical differences according to Tukey ˂ 0.05.
The plant growth promoting effect of Rhizobium phaseoli in P. vulgaris coincides with that reported by Yadegari (2008); Moreno et al. (2007). Other studies indicate that Rhizobium spp., increases the nodulation in the roots and provides greater nitrogen fixation, productivity and fertility in the crops, even under adverse soil conditions, providing an alternative to be used as a replacement for nitrogen fertilizers (Solaiman et al., 2005; Bhattacharjee and Sharma, 2012).
These results also coincide with those of Colas et al. (2014) who mention that all plants co-inoculated with Rhizobium spp., produce higher yields and numbers of nodules than in non-inoculated ones, which demonstrates the potential of the use of mixed inoculate to improve the productivity of common bean.
Conclusions
The use of symbiotic inoculants of the Rhizobium phaseoli type has the potential to reduce and prevent the damages caused by Xanthomonas axonopodis and the appearance of symptoms associated with the disease in the culture, such as chlorosis and common bacterial blight of beans, considered an advantage to avoid the use of bactericides and chemical nitrogen fertilizers. The use of microbial agents could be an alternative in bean cultivation due to its activity as a promoter of plant growth and solve problems of a nutritional and phytosanitary nature.
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Received: January 2019; Accepted: March 2019
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