Coffee Coffea spp. (Gentianales: Rubbiaceae) is native to Ethiopia, the introduction in Mexico was about 1790 ^{Medina et al. (2016)}. The main coffee producing countries in the world are Brazil with a production of 3 019 051 t per year, Vietnam with 1 460 800 t, Colombia with 745 084 t, Indonesia with 639 305 t, Ethiopia with 469 091 t, Honduras 362 367 t, India with 348 000 t, Peru with 277 760, Guatemala with 236 145, Uganda with 203 535 and Mexico with a production of 151 714 t year^-1 (^{FAO, 2016}).

In Mexico, the cultivation area is 700 000 ha, Chiapas, Veracruz, Puebla and Oaxaca, the main producing states, which together contribute nearly 90% of production (^{SIAP, 2016})

In our country, Coffea arabica L. occupies a little more than 97% of the coffee area, while the species C. canephora Pïerre is located in the rest of the area (^{AMECAFE, 2012}).

Phytoparasitic nematodes constitute one of the main phytosanitary problems that affect the cultivation of coffee (Coffea arabica L.), cause yield reductions ranging between 10 and 70% (^{Gomez and Rivera, 1987}). Numerous species of nematodes have been reported in association with this crop; however, the species of the genera Meloidogyne and Pratylenchus are the most widely distributed and those that cause the greatest losses in the crop (^{Inomoto et al., 1998}; ^{Herrera and Marban, 1999}; ^{Oliveira et al., 1999}; ^{Campos and Villain, 2005}).

Nematodes represent a phytosanitary problem that is increasing and unfortunately few products are available for their control, according to the above the objective of the research was to evaluate the biological effectiveness of nematicide of biological origin Nemmax, for the control of nematodes Meloidogyne incognita, Pratylenchus spp. and Dorylaimus spp., associated with the cultivation of coffee.

Location of the experiment: the study was carried out in coffee plants of the Catuai commercial variety with a history of nematodes, located in Soconusco, municipality of Motozintla, Chiapas. The laboratory part was carried out at the Antonio Narro Autonomous Agrarian University (UAAAN), in Saltillo, Coahuila.

Products: the Nemmax product, of biological origin with nematicidal activity contains a mixture of extracts of vegetable origin 40% (40 g L^-1), rhizogenic bacteria Bacillus spp. and Pseudomonas spp. 1x10⁸ UFC mL^-1 10% (10 g L^-1), available phosphorus 2% (2 g L^-1), zinc 1% (1 g L^-1), chelating agents 1.5% (1.5 g L^-1), fulvic acids 500 ppm ( 0.5 g L^-1), diluents and conditioners 45 (45 g L^-1) and the commercial product Nemacur 400 CE (Fenamiphos) with an equivalent to 35 g of IA L^-1 of product.

Experimental design: a randomized block design was used, with 5 treatments and 5 repetitions. Each experimental unit consisted of two 2-year-old coffee bushes, with a planting distance of 1 m in length, the distance between blocks was 2 m and the useful plot was 2 bushes. An analysis of variance (Anova) and a mean comparison test by Tukey (p= 0.05) were performed.

Initial sampling: a previous sampling was carried out in two coffee bushes per experimental unit, in order to know the population of nematodes present. A sample of 1 kg of soil of the silty migajon type was taken at the north cardinal point at a depth of 40 cm and was transferred to the UAAAN Nematology laboratory. From each sample of 1 kg of soil, a subsample of 100 g of soil was taken to be processed by the Baerman funnel methodology (^{Kleynhans, 1999}). The nematodes obtained were identified by means of stereoscopic and compound microscope observations and taxonomic keys.

Field trials: prior to the application of the product Nemmax and Nemacur^® 400 CE, a trench was opened around the drip zone of each experimental coffee, in order to bring the product into contact with the root system. The products were applied with a manual sprayer, with capacity of 15 L brand Solo^® later covered with soil.

During the experiment, the same agronomic management was carried out, such as the control of weeds and foliar diseases. The Nemacur^® 400 CE nematicide was applied once in the mooring phase of the coffee cherry, at the beginning of the establishment of the experiment and the Nemmax nematicide was applied three times, the first in the fruit mooring stage and the others with intervals of 15 days between each application.

Final sampling of the nematode population: at 120 days after the last application of the products, in each of the experimental units (two trees), a sample of 1 kg of soil was taken from each coffee bush, at the point North cardinal, in the drip zone to a depth of 40 cm. the two sub-samples were mixed and only a representative sample of 1 kg of soil was taken. The 25 samples were sent to the UAAAN Nematology Laboratory, 100 g of soil of each one were analyzed, by the Baerman funnel technique (^{Kleynhans, 1999}). Nematodes present with emphasis on the genera Meloidogyne incognita, Pratylenchus spp. and Dorylaimus spp. they were identified under the stereoscopic and compound microscopes and the taxonomic keys.