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Revista mexicana de ciencias agrícolas

versão impressa ISSN 2007-0934

Rev. Mex. Cienc. Agríc vol.8 spe 19 Texcoco Nov./Dez. 2017

https://doi.org/10.29312/remexca.v0i19.672 

Articles

Impacts to avocado ‘Hass’ fruit in the packaging line and its effect on post-harvest quality

Juan Antonio Herrera-González1 

Samuel Salazar-García2  § 

1Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias-Campo Experimental Uruapan. Avenida Latinoamericana 1101, Uruapan, Michoacán, México. CP. 60150. Tel. (55) 8718700, ext. 84202.

2Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias-Campo Experimental Santiago Ixcuintla. Carretera Internacional México-Nogales km 6, Entrada a Santiago Ixcuintla, Nayarit. Tel. (55) 8718700, ext. 84426.


Abstract

Harvest, postharvest, storage and distribution processes of the avocado ‘Hass’ fruit must maintain the quality of the fruit because in these processes the fruit is exposed to impacts that cause quality losses. The objectives of this study were: 1) to quantify the magnitude and sites where the impacts occur in the selection line; 2) to determine the damages that generate the impacts in the external and internal quality of the fruits; and 3) to evaluate the effect of the types of impact and the refrigeration on the quality of the fruit in maturity of consumption. A wireless sensor with an avocado fruit was used, which measures the impacts and transmits them to a laptop that counts in real time the number of impacts and their magnitude. The sensor was passed through the selection line of four exporting balers, the process was separated into four stages and the speed of the fruit strip was varied. We also evaluated the effect of induced impacts on fruit quality at maturity of consumption, after storage at room temperature or in refrigeration. The maximum impacts differed between packers and occurred in the fruit emptying stage; in addition, a low speed in the emptying band did not reduce the intensity of the impacts. Only fruits stored for 28 days in refrigeration (with induced impacts) showed damages to their internal quality at maturity of consumption.

Keywords: Persea americana Miller.; mechanical damage; wireless impact sensor; post-harvest handling

Resumen

Los procesos de cosecha, poscosecha, almacenamiento y distribución del fruto de aguacate ‘Hass’ deben mantener la calidad del fruto ya que en estos procesos el fruto está expuesto a impactos que ocasionan pérdidas de calidad. Los objetivos de este estudio fueron: 1) cuantificar la magnitud y sitios donde ocurren los impactos en la línea de selección; 2) determinar los daños que generan los impactos en la calidad externa e interna de los frutos; y 3) evaluar el efecto de los tipos de impacto y la refrigeración sobre la calidad del fruto en madurez de consumo. Se utilizó un sensor inalámbrico con forma de fruto aguacate, que mide los impactos y los transmite a un ordenador portátil que contabiliza en tiempo real el número de impactos y su magnitud. Se hizo pasar el sensor por la línea de selección de cuatro empacadoras exportadoras, se separó el proceso en cuatro etapas y se hizo variar la velocidad de la banda de vaciado de fruto. También se evaluó el efecto de impactos inducidos sobre la calidad del fruto en madurez de consumo, después de su almacenamiento a temperatura ambiente o en refrigeración. Los impactos máximos difirieron entre empacadoras y ocurrieron en la etapa de vaciado de frutos; además, una baja velocidad en la banda de vaciado no disminuyó la intensidad de los impactos. Sólo los frutos almacenados por 28 días en refrigeración (con impactos inducidos) mostraron daños a su calidad interna en madurez de consumo.

Palabras clave: Persea americana Miller.; daño mecánico; manejo poscosecha; sensor inalámbrico de impactos

Introduction

The avocado ‘Hass’ fruit is susceptible to impacts that occur during its post-harvest handling. Impacts on the baler can be an important source of mechanical damage (Yull et al., 2015). In the selection and packaging line the fruit is exposed to dynamic and static impacts (vibration and compression) that demerit the internal quality of the fruit at maturity of consumption (Pallottino et al., 2009). The impacts or mechanical damages cause economic losses since they demerit the commercial value of the fruit (Sablani et al., 2006). The result of this is rot and alteration in the texture, color and flavor of the fruit pulp, caused by the acceleration of maturation processes, decreasing firmness and loss of mass, shortening shelf life (Opara and Pathare, 2014).

In Mexico, avocado ‘Hass’ for export goes through various selection processes, from harvest to packaging. This exposes the fruit to impacts of various magnitudes that may decrease its post-harvest quality (Van-Linden et al., 2006). Impacts result from the excessive force of an impact on a hard surface or against other fruits that fail to absorb the force of such impacts or compressions (Yurtlu and Erdoǧan, 2005). The above generally results in damage to the internal quality of the fruit when it reaches the maturity of consumption (Van-Linden et al., 2008).

Several investigations have focused on understanding the cause and mechanisms associated with damage by impacts to the fruit. For example, in citrus and apples, the magnitude of impacts and vibrations has been studied (Bollen et al., 2001), the influence of materials and containers, impacts among fruits, susceptibility to mechanical damage and relationship of the previous factors with fruit quality at maturity of consumption (Van-Zeebroeck et al ., 2007). However, for fresh fruits, the major challenge is the detection and prevention of impacts on the selection-packaging line (Jarimopas et al., 2007). This is because inspection, detection and adjustments to machinery, materials and containers are performed by non-standardized maintenance personnel, leading to inappropriate and inconsistent corrections.

There is an instrument equipped with a triaxial accelerometer to measure the impacts on the selection-pack line. It can be used with an avocado-type pseudofruit, with the same physical and mechanical properties during post -harvest management (Opara and Pathare, 2014), which can simulate the path of fruits through the selection-packaged line (Yull et al., 2015). The triaxial accelerometer is a wireless sensor installed inside the pseudo-fruit that detects and transmits data wirelessly and in real time, allowing inspections, corrections and adjustments to the selection-packing lines (Toivonen et al., 2007).

Although information have been developed on the effects of mechanical impacts and damages on post-harvest quality on apple fruit (Van-Zeebroeck et al., 2007), tomato (Desmet et al., 2004) and citrus fruits (Miller et al., 1991), among others, in avocado the information on this type of damages is scarce. The objectives of this research were 1) to quantify the magnitude and sites where the impacts occur in the line of selection and packing of avocado ‘Hass’; 2) to determine the damages that generate the impacts in the external and internal quality of the fruits; and 3) to evaluate the effect of the types of impact and the refrigeration on the quality of the fruit in maturity of consumption.

Materials and methods

Study 1. Magnitude and places where impacts occur in the packaging line

Comparison of balers. In September 2012 four export-certified avocado ‘Hass’ packers representing 10% of certified packers were selected. The balers have the same technological level in the selection and packing process and were identified as baler 1, baler 2, baler 3 and baler 4.

The detection and quantification of impacts was performed with an avocado sensor (Wireless Sensor Inc., PE, Ontario, Canada), which records and transfers data wirelessly to a mobile device (Palm®). The sensor uses a battery of current supply and has a triaxial accelerometer that measures the impacts in Newtons (N; 9.81 N= 1 kgF) and transmits the measurements through radiofrequency to the portable computer, which counts the number of impacts and its magnitude in real time.

In each packer the sensor was run for 18 cycles throughout the packaging line (Figure 1) and each cycle was taken as a repeat. The data obtained were: number of total impacts, maximum impact average and average intensity of total impacts.

Figure 1 Stages of the selection and packaging process of avocado ‘Hass’ for export: A) emptying of fruits; B) rollers and brushes; C) manual selection, singulator and labeling; and D) fall to the hopper and hopper. 

Comparison between selection and packing stages. The complete cycle of the process was divided into four stages (Figure 1). The wireless sensor was passed for 10 cycles at each stage. The same data described in the previous section were obtained.

Speed of the fruit strip. Three speeds, 24, 40 and 50 Hertz (Hz) were used. At each speed the wireless sensor was passed 10 times along with the fruits in the emptying band (Figure 1A). The data obtained were treated as in previous evaluations.

Study 2. Effect of impacts on external and internal quality of fruit at maturity of consumption

In this study, impacts were induced on different surfaces and from different heights (Table 1). In 2013, ‘Hass’ fruits were harvested during the main flowering (spring 2012) with 32-40% dry matter in the pulp.

Table 1 Treatments to evaluate the impact of the impacts on the external and internal quality of the avocado ‘Hass’ fruit at maturity of consumption.  

Trat Superficie Altura (m) Trat Superficie Altura (m) Trat Superficie Altura (m)
1 Madera+Dulona 0.5 9 Metal+Dulona 0.3 17 Otros frutos 0.2
2 0.7 10 0.4 18 0.4
3 0.9 11 0.5 19 0.6
4 1.1 12 0.6 20 0.8
5 Placa de madera 0.6 13 Metal liso 0.3 21 Tubos PVC 0.2
6 0.7 14 0.5 22 0.4
7 0.9 15 0.7 23 0.6
8 1.1 16 0.9 24 0.8

For each treatment, 20 fruits were used and the impacts were induced by letting them fall freely. After the impact the fruits were stored at room temperature (23 °C ±2 °C and relative humidity 70% ±10%) until consumption maturity. At maturity of consumption the proportion of fruits with external damage (rot) was quantified. Something similar was done to evaluate the internal quality of the fruit, although in this case each fruit was opened in half. Other internal disorders, such as pulp discoloration, vascular darkening, and basal rot were not considered, as they are not caused by impacts on fruits (Bill et al., 2016).

Study 3. Effects of impact types and refrigeration on fruit quality at consumption maturity

Similar to study 2, impacts to 20 fruits were induced by treatment, allowing them to fall freely from three different heights on the surfaces identified in study 2 as those that caused damages to the external and internal quality of the fruit (Table 2). To measure the magnitude of the impact to which the fruit was subjected, the sensor was dropped 30 times on the same surfaces and heights.

Table 2 Treatments to induce impacts and evaluate their effect on post-harvest quality of avocado ‘Hass’ fruit. 

Trat Material/altura (m) Trat Material/altura (m)
1 Metal liso 0.4 9 Tubos de PVC/0.6
2 Metal liso/0.5 10 Frutos/0.4
3 Metal liso/0.6 11 Frutos/0.5
4 Placa de metal recubierta*/0.4 12 Frutos/0.6
5 Placa de metal recubierta/0.5 13 Tubo de metal/0.4
6 Placa de metal recubierta/0.6 14 Tubo de metal/0.5
7 Tubos de PVC/0.4 15 Tubo de metal/0.6
8 Tubos de PVC/0.5

*= material de 10 mm de espesor que amortigua impactos.

After the impact, the fruits of each treatment were refrigerated (5 °C ± 2 °C; 85% ± 10% relative humidity) for 14 and 28 days. At the end of each refrigeration period, 10 fruits of each treatment were transferred to room temperature (22 °C ± 2 °C y 75% ± 10% HR) until their consumption maturity.

At the end of refrigeration (days 14 and 28) and in maturity of consumption, the treatments were evaluated for the decrease of firmness (kgF) with a texturometer (Universal Xtrad, model A-XT2t, Serqueux, France) and loss of mass (PDM) of fruits with a digital scale (Ohaus, CS2000, Parsippany, USA). In consumer maturity the internal quality of the pulp was evaluated with a scale where the categories were: 0= 0%; 0.5= 5%; 1= 10%; 1.5= 15%; 2= 25%; 2.5= 30%; and 3= 35% pulp with darkening and air cavities in the fruit pulp (White and Woolf, 2005).

Statistic analysis

For the study one was used a completely randomized experimental design and in the two and three we used a bifactorial analysis in completely random design. The comparison of means in the three studies was performed with the Waller-Duncan test (p= 0.05). The variation factors in study 1 were: packer, stage and speed of fruit emptying band. In study 2 were the drop height and the impact surface. For study 3 were the drop height, impact surface and the cooling days. The analysis of variance for the variables evaluated was done with the statistical package SAS for Windows V9.2 (SAS, 2008).

Results and discussion

Study 1. Magnitude and sites where impacts occur in online packaging

Comparison of balers

The total number of impacts of any intensity (0.1-25 kgF) recorded in the selection and packing line was similar in all four packers (Table 3). This was independent of the duration of the process, since in some balers the process lasts just under 4 min, while in others it lasts around 6 min. The average of the maximum impacts recorded by the sensor was different between the packers (p< 0.05). Packer 1 was the one with the highest value impact. The baler with the maximum impact of lower average was the baler 4. The balers 2 and 3 had intermediate values.

Table 3 Differences between avocado ‘Hass’ packers for the impacts recorded by the wireless sensor on the packaging lines. 

Empacadora Número de impactos Promedio impacto máximo (kgF) Promedio intensidad de impactostotales (kgF)
1 42.7 9.9 a 1.6 a
2 42.4 8.1 ab 1.3 b
3 42.1 7.4 bc 1.1 bc
4 37.3 5.8 c 1 c
Pr>F 0.2663 0.0046 <0.0001

Medias con letras iguales no son estadísticamente diferentes (Waller-Duncan, p≤ 0.05).

The average of total impacts that the sensor registered in the packers evaluated was different (p< 0.05). The packer 1 had the highest average and the lowest was for packer 4 (Table 3). The low average of the total impacts was due to the fact that the sensor recorded a large number of impacts with values lower than 1 kgF and few impacts greater than 5 kgF.

Comparison between selection and packing line stages

The number of impacts, the maximum and average impact of total impacts recorded by the sensor was different between the packing stages. Stage 2 was where the greatest number of impacts were recorded and in stages 1 and 4 there were fewer impacts (Table 4). Although there were statistical differences (p< 0.05), high intensity impacts can occur at any stage of packaging. However, in stage 1, impacts of higher value occur (Table 4). The mean total impact recorded by the sensor in the evaluated stages was different (p< 0.05). Stage 1 (fruit emptying) was the highest average intensity and stage 2 (rollers and brushes) recorded the lowest average intensity of total recorded impacts. Although in the stage of emptying of fruit has the smallest number of impacts, it is where the fruit suffers more maximum impacts with greater intensity.

Table 4 Differences in the number and intensity of impacts received by avocado ‘Hass’ fruit in the stages of the packaging line in four packing machines. 

Etapa Núm. de
impacto
Promedio
impactos máximo (kgF)
Promedio intensidad de
impactos totales (kgF)
1 6.1 c 8.5 a 2.9 a
2 45.6 a 6.7 ab 1 c
3 14.9 b 6.6 b 1.9 b
4 7.6 c 6.0 b 1.9 b
Pr>F <0.0001 0.026 <0.0001

Medias con letras iguales no son estadísticamente diferentes (Waller-Duncan, p≤ 0.05).

Fruit stripping speed

The number of impacts recorded between the speeds evaluated was similar and was between 6.1 impact on the highest speed and 3.5 impacts on the lowest speed. The average maximum impact and the average total impacts were also similar among the three velocities evaluated. The maximum intensity impact was between 6.3 and 8.6 kgF. The average of the total impacts recorded was between 2.4 and 3.9 kgF (p> 0.05). This indicates that, even if the speed of the drainage belt is reduced, the height of falling of the fruits remains the same, so reducing the speed does not reduce the intensity of the impacts.

Yull et al. (2015) reported differences in the number of impacts in three apple packers, with 50, 42 and 28 impacts respectively. In orange packers, they reported 89 impacts on average. Although the packaging process for avocados, apples and oranges has differences, in avocado there were no differences between packers as presented in apple packers. The number of impacts to which both fruits are exposed are similar, around 40 impacts. Although the number of impacts between avocado and apples packers was similar and in oranges was higher, the impact intensity was lower in apples and oranges, as reported by Ferreira et al. (2005).

While in avocado the impact of greater intensity was of 9.9 kgf, in apples and oranges barely reached 3 kgf. In another study done in tomato packers, impacts between 5 kgF and 30 kgF were recorded using a triaxial accelerometer (Ferreira et al., 2005). The difference between packers may be due to the sensitivity of the fruits to the impacts due to the anatomical and morphological conformation of the fruit, but also the design of the packaging line.

However, it is not surprising that the packing process in tomato (Ferreira et al., 2005), peach (Ahmadi et al., 2010), apple (Yull et al., 2015) and citrus (Miller et al., 1991) is different. However, it has been reported that the stage of fruit emptying is the stage with high intensity impacts throughout the fruit packing process, similar to what was found in the present investigation. In avocado the stage of emptying of fruits is realized with turning machines that drop the fruits on a cushioned surface of 0.4 m of length and takes to the fruits to a band of rollers of plastic or metal, reason why the shock of the fruits with the rollers causes the impacts. Lowering the speed of the machine reduces the number of impacts, but not the intensity.

Study 2. Detection of the effects of impacts on the internal quality of the fruit avocado ‘Hass’

No treatment damaged the skin of the fruit so that the induced impacts did not affect its external quality. However, the internal quality of the fruit was affected by the type of impact of each treatment (p> 0.05) (Figures 2 and 3).

Figure 2 Darkening and air cavities in the avocado ‘Hass’ pulp caused by induced impacts. 

Figure 3 Effect of treatments evaluated (Table 1) on the proportion of darkening fruits and air cavities in the pulp at maturity of consumption. 

The treatments with wood and wood + dulona plates were those that did not affect the internal quality of the fruit at maturity of consumption. The same happened with treatments 13 and 14 with smooth metal (Table 5). The rest of the treatments presented values higher than 5% of fruits with damages, the highest being those of metal surface with the highest heights (treatments 15 and 16), this because harder and more compact materials have an effect on the intensity of the impact and is increased with the height of the fall.

Table 5 Internal quality of refrigerated fruits (5 °C ±2 °C and 85% ±10% HR) for 14 and 28 days and transferred at room temperature (22 °C ±2 °C and 75% ±10% HR) until maturity of consumption. 

Tratamiento Tiempo en refrigeración
14 días 28 días
1 0.15 a 0.65 a
2 0 a 0.4 ab
3 0.1 a 0.33 abc
4 0 a 0.27 bcd
5 0.05 a 0.26 bcd
6 0.05 a 0.25 bcd
7 0 a 0.2 bcd
8 No se realizó 0.2 bcd
9 0 a 0.17 bcd
10 0.1 a 0.11 bcd
11 0.1 a No se realizó
12 0 a 0.05 cd
13 0 a 0.05 cd
14 0.1 a 0.05 cd
15 0.05 a 0 d
Pr>F 0.086 a <0.0001

Medias con letras iguales no son estadísticamente diferentes (Waller-Duncan, p≤ 0.05).

The results of the study indicate that the impacts to internal quality of the fruit become a problem in consumer maturity, where consumer satisfaction is affected by fruit quality. The consumer judges the quality of the fruit based on its appearance and reduces acceptance if there is damage (Oprara et al., 2014). The most important impacts in the selection and packing processes in some fruits are those that occur between fruits, as well as falling and impacting with machine surfaces (Van-Zeebroeck et al., 2007) as occurred in the present study. Although the intensity of impacts is not reported in some studies, the effect on quality deterioration at maturity is mentioned (Ahmadi et al., 2010).

Study 3. Effects of the magnitude of the impact and the cooling on the quality in maturity of consumption of ‘Hass’

Fruits refrigerated for 14 days

At 14 days of cooling the firmness of the fruits was different between treatments at the end of the cooling and at maturity of consumption (p< 0.05). The fruits of treatment 2 presented the lowest firmness at the end of the cooling and at maturity of consumption (mooth metal/0.5 m). The fruits of treatments (0.6 m), coated metal plate (0.6 m), PVC (0.6 m) tubes, fruits (0.5 m) and metal tube (0.5 m) were more firm at the end of the treatment refrigeration and consumption maturity. But treatment 1 was the strongest at maturity of consumption. The treatments covered metal plate (0.4 m), coated metal plate (0.5 m), PVC tubes (0.4 m), fruits (0.6 m), metal tube (0.4 m) and metal tube (0.6 m) in consumption maturity presented a firmness of 1 kgF (Figure 4). Although at the end of the 14 days of refrigeration the loss of mass was similar, at maturity of consumption it was different (p< 0.05), but no treatment exceeded 8% of loss.

Figure 4 Average firmness and mass loss of refrigerated fruits (5 °C ±2 °C and 85% ±10% HR) for 14 days (a); and after being transferred to room temperature (22 °C ±2 °C and 75% ±10% HR) until consumption maturity(b). 

At maturity of consumption, the internal quality of the fruit was similar between treatments (p< 0.05). Metal treatments (0.5 m), coated metal plate (0.4 m), PVC pipes (0.4 m), PVC pipes (0.6 m), fruits (0.6 m) and metal tube (0.4 m) were not damaged in the internal quality. However, the remaining treatments showed darkening and small air cavities in the pulp (Table 5).

Fruits refrigerated for 28 days

At the end of the 28 days of refrigeration and at maturity of consumption the firmness was different (p< 0.05) among the treatments evaluated. At the end of the cooling the firmness of all the treatments was below 4 kgf (close to the firmness of maturity of consumption, 1.5 kgF) and in maturity of consumption the firmness of all the fruits was less of 1.2 kgf, this firmness reached three days after the transfer at room temperature (Figure 5). The loss of mass, at the end of 28 days of refrigeration and at maturity of consumption, was similar between treatments. At the end of the cooling (day 28) the majority of the treatments had exceeded 5% of mass loss and in maturity of consumption exceeded 10%. Although no differences were found in the loss of mass, these percentages at maturity of consumption cause fruit wilt (Herrera- González et al., 2013) (Figure 5). In consumer maturity the internal quality was differentiated between the treatments (p< 0.05). With the exception of treatment 15 (metal tube, 0.6 m), the other treatments presented darkening and air cavities in the fruit pulp in different degrees (Table 5).

Figure 5 Mean of firmness and mass loss of refrigerated fruits (5 °C ±2 °C and 85% ±10% HR) for 28 days (a); and transferred at room temperature (22 °C ±2 °C and 75% ±10% HR) until consumption maturity (b). 

The loss of firmness, mass and internal quality was more evident in the fruits stored for 28 days. The long period in refrigeration and the induced impact affected the physiology of the fruit (Pedreschi et al., 2016), accelerating the enzymatic activity and the degradation of the protopechins (Zhou et al., 2007). Although in pre-harvest, crop management, fertilization and crop maturity confer greater firmness, tolerance to mechanical stress and less dehydration, long periods of refrigeration render the fruit less tolerant and more susceptible to mechanical damage and physiological disorders (Pasini et al., 2004).

Evaluation scale 0= 0%; 0.5= 5%; 1= 10%; 1.5= 15% pulp with darkening and air cavities in the fruit pulp (White and Woolf, 2005).

Conclusions

The exposure of the avocado ‘Hass’ fruits to the impacts in the selection line was similar between the packers evaluated. However, some packers had stronger impacts than others. The stage in which the impacts of greater intensity occurred was the band of emptying of fruits and the reduction of the speed of this band did not diminish the quantity and the intensity of the impacts. Induced impacts (similar to those occurring in the packers) did not affect the external quality of the fruit, but the internal quality becomes evident until the maturity of consumption. The damage was characterized as darkening and air cavities in the pulp. Long periods of cooling affect the quality of the fruits when they are exposed to impacts of any magnitude and on any surface.

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Received: July 00, 2017; Accepted: August 00, 2017

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