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Revista odontológica mexicana
Print version ISSN 1870-199X
Rev. Odont. Mex vol.13 n.4 Ciudad de México Dec. 2009
https://doi.org/10.22201/fo.1870199xp.2009.13.4.15558
Trabajos originales
Efecto antimicrobiano del agua potencialmente oxidativa
Antimicrobial effect of the oxidative potential water
*Alumno del 2do año de la Maestría en Endodoncia de la Facultad de Estomatología. Universidad Autónoma de San Luis Potosí.
§Jefa del Laboratorio de Investigación de la Maestría en Endodoncia de la Facultad de Estomatología. Universidad Autónoma de San Luis Potosí.
IIProfesor-Investigador del Instituto de Metalurgia. Universidad Autónoma de San Luis Potosí.
¶Coordinador de la Maestría en Endodoncia de la Facultad de Estomatología. Universidad Autónoma de San Luis Potosí.
**Profesor-Investigador de la Facultad de Estomatología. Universidad Autónoma de San Luis Potosí.
El agua potencialmente oxidativa (APO) es una solución activada por un proceso electrolítico que tiene aplicaciones en el área de la salud.
Objetivo
El objetivo del estudio fue evaluar el efecto antimicrobiano del APO en concentraciones de NaCl al 1% y al 2.5% contra cepas de Enterococcus faecalis aisladas de conducto radicular.
Metodología
Se utilizaron cepas de E. faecalis aisladas de conducto radicular de pacientes con indicación de retratamiento, con presencia de lesión periapical y diagnóstico de periodontitis apical crónica. Se utilizó tioglicolato-prerreducido (BD BBL) como medio de transporte y como medio de cultivo cajas de agar sangre CDC (BD BBL) en condiciones de anaerobiosis. Para evaluar la solución irrigante se utilizó la técnica de dilución serial. Se dividieron 86 muestras en 4 grupos experimentales de APO (1% y 2.5%), con un esquema de irrigación de 9 mL de APO + 1 mL de inóculo con un tiempo de exposición al irrigante de 5 minutos. Los tiempos de incubación para todas las muestras fueron de 24 y 48 h. Como control positivo se utilizó NaOCl 5.25% como irrigante y en el control negativo agua destilada.
Resultados
Se demostró efectividad del APO en concentración de NaCl (Fermont) al 1 y 2.5%, obteniendo un promedio de 28 unidades formadoras de colonias (UFC) y de 2 UFC para el APO al 1 y al 2.5%, respectivamente.
Conclusiones
El APO tiene mayor efecto antimicrobiano en ambas concentraciones de NaCl utilizadas (1 y 2.5%) en comparación con el control. Sin embargo, es necesario realizar estudios que evalúen la eficacia de éste sobre el biofilm bacteriano, así como su biocompatibilidad para proponer su uso como irrigante intraconducto.
Palabras clave: Agua potencialmente oxidativa; E. faecalis; efecto antimicrobiano
The oxidative potential water (OPW) is an electro-chemically activated solution and it is widely used in health fields.
Objective
The objective was to evaluate the antimicrobial effect of the OPW in concentration of 1% and 2.5% of NaCl against E. faecalis isolated from root canal.
Methodology
E. faecalis samples isolated from patients root canals retreatment with an apical lesion and diagnosis of chronic apical periodontitis were used. The samples were incubated in anaerobic conditions in CDC blood agar medium until their use. The serial CDC technique was used to evaluate the root canal irrigant. Eighty six samples were divided in 4 experimental groups of OPW (1% and 2.5%), with an irrigation scheme of 9 mL of OPW + 1 mL of inoculate and with 5 minutes of exposition to the irrigant. The incubation times for all samples were of 24 and 48 hours. In the positive control, 5.25% NaOCl was used and in the negative control, distilled water.
Results
The OPW was effective in concentrations of 1% and 2.5% NaCl, obtaining an average of 28 CFU and 2 CFU for 1% and 2.5% OPW, respectively.
Conclusions
The OPW has an antimicrobial effect in the concentrations used of NaCl (1% and 2.5%). Nevertheless, it is necessary to study the efficacy of this irrigant on the bacterial biofilm and its biocompatibility in order to propose it, as a root canal irrigant.
Key words: Oxidative potential water; E. faecalis; antimicrobial effect
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