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Revista odontológica mexicana
Print version ISSN 1870-199X
Rev. Odont. Mex vol.13 n.1 Ciudad de México Mar. 2009
https://doi.org/10.22201/fo.1870199xp.2009.13.1.15614
Trabajos originales
Morphological and phenotypic differences in fibroblasts obtained from gingival overgrowth secondary to phenytoin: A pilot study
Diferencias morfológicas y fenotípicas en fibroblastos obtenidos de sobreagrandamiento gingival por fenitoína: un estudio piloto
*Centro de Investigaciones Odontológicas, Facultad de Odontología, Pontificia Universidad Javeriana, Bogotá D.C, Colombia.
§Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá D.C., Colombia.
IIGrupo de Ciencias Básicas Médicas, Facultad de Medicina, Universidad de los Andes, Bogotá D.C, Colombia.
Introduction:
Gingival fibroblasts seem to be key cells in the pathogenesis of gingival overgrowth secondary to phenytoin intake. These cells not only participate in the extracellular matrix protein synthesis, but also regulate protein degradation through production of collagenolitic enzymes and phagocytosis. Some growth factors and cytokines such as IL-1α, TGF-β and TNF-α participate in the homeostasis of extracellular matrix.
Objective:
The aim of this study was to evaluate the presence of IL-1 α, TGF-β and TNF-α receptors on human gingival fibroblasts from a healthy donor, a patient with gingival overgrowth consuming phenytoin (responder) and a donor consuming phenytoin without gingival overgrowth (non- responder).
Methods and results:
Primary cell cultures of gingival fibroblasts from each donor were characterized by flow cytometry demonstrating vimentin and CD14 positive cells. Using size detector, gingival fibroblasts from the responder donor were larger than fibroblasts from non-responder and healthy donor. A low but positive expression of IL-1R and TGF- βR was detected in cells from the responder donor. TNF- αR expression was detected in all three types of cultured cells but it was higher in the responder donor.
Conclusions:
These results suggest differences in the morphologic and phenotypic characteristics between gingival fibroblasts from the donors studied.
Key words: Gingival fibroblast; cytokine; phenytoin
Introducción:
Los fibroblastos gingivales son considerados como células claves en la patogénesis del agrandamiento gingival secundario al uso de medicamentos como la fenitoína. No solamente participan en la síntesis de proteínas de matriz extracelular, sino también en su degradación mediante la producción de enzimas colagenolíticas y fagocitosis. Algunas citocinas como la IL-1α, TGF-β y TNF-α participan en la homeostasis de la matriz extracelular.
Objetivo:
El objetivo de este estudio fue evaluar la presencia de receptores para IL-1α, TGF-β y TNF-α en fibroblastos gingivales humanos de un donante sano, un paciente con agrandamiento gingival consumidor de fenitoína (respondedor) y un donante consumidor de fenitoína sin agrandamiento gingival (no respondedor).
Metodología y resultados:
Cultivos primarios de fibroblastos gingivales obtenidos de cada paciente fueron caracterizados por citometría de flujo demostrando células positivas para vimentina y CD14. Usando el detector de tamaño por citometría, los fibroblastos gingivales del paciente respondedor mostraron mayor tamaño que las células de los otros sujetos. Se detectó una expresión positiva pero baja para IL-1R y TGF- βR en células del donante respondedor. La expresión del TNF- αR fue detectada en los tres tipos de poblaciones celulares, pero ésta fue mayor en fibroblastos del donante respondedor.
Conclusión:
Estos resultados sugieren diferencias morfológicas y fenotípicas entre las poblaciones de fibroblastos gingivales estudiados.
Palabras clave: Fibroblasto gingival; citocinas; fenitoína
Acknowledgements
The authors thank Dr. Nelson Contreras for his continued support, Drs. Luz K. Sánchez, Andres Pinto, and Camilo Durán for the reading of the manuscript, Bogotá's Liga Central Contra la Epilepsia and to the patients who participated in this study. Special thanks to K.C Dowdell and L. Hamo, School of Medicine, USC, Los Angeles, for correcting the manuscript. This work was founded by the Dental Research Center, School of Dentistry, Pontificia Universidad Javeriana.
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