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Revista odontológica mexicana
versión impresa ISSN 1870-199X
Rev. Odont. Mex vol.12 no.4 Ciudad de México dic. 2008
https://doi.org/10.22201/fo.1870199xp.2008.12.4.15625
Trabajos originales
Efecto del lipopolisacárido (LPS) y el ácido lipoteicoico (LTA) sobre la ciclooxigenasa2־ (COX-2) en células pulpares humanas
Lipopolysaccharide (LPS) and lipoteichoic acid (LTA) effects on cyclooxygenase-2 in human dental pulp cells
*Laboratorio de Bioquímica, DEPeI de la FO. UNAM.
Las toxinas bacterianas como el lipopolisacárido (LPS) y el ácido lipoteicoico (LTA), están presentes en bacterias Gram-negativas y Gram-positivas, respectivamente. Éstas originan el proceso inflamatorio pulpar. En este estudio, se investigo el efecto de LTA y LPS sobre la expresión de la enzima ciclooxigenasa-2 en células pulpares humanas. Se partió de la hipótesis de que estas moléculas participan en el proceso inflamatorio pulpar a través de la sintesis de la ciclooxigenasa-2 en células pulpares humanas. Para evaluar esta hipótesis, se estudió el efecto del LPS y el LTA en cultivos primarios de células pulpares humanas. Finalmente, se caracterizaron las vías y moléculas involucradas en la expresión de COX-2. En las células pulpares humanas tratadas con LTA o LPS, se detectó que estas moléculas promueven la expresión de COX-2. Por otro lado se encontró que la expresión de COX-2 se ve bloqueada por tratamientos con SB203580, PD98059 y estaurosporina. Los resultados demostraron que el tratamiento con LTA y LPS promueven la expresión de COX-2 en eventos donde participan las proteínas cinasas p38, ERK y PKC. Los hallazgos son importantes porque COX-2 está involucrada en la síntesis de PGE2, involucrada en el establecimiento de procesos inflamato rios crónicos. Finalmente se caracterizó la naturaleza de un mediador implicado en el proceso de una pulpitis.
Palabras clave: Ácido lipoteicoico; LTA; lipopolisacárido; LPS; ciclooxigenasa; COX-2; prostaglandina E2; PGE2; ERK; proteína cinasa regulada extracelularmente; PKC; proteína cinasa C; JKN cinasa aminoterminal reguladora del factor de transcripción C-Jun; milimolar (mM)
Bacterial toxins such as lipopolysaccharide (LPS) and lipoteichoic acid (LTA) can be found in Gram-negative and Gram-positive bacteria, respectively. This toxins cause an inflammatory process denominated pulpitis. In the present study, the effect of LTA and LPS on the expression of cyclooxygenase-2 in human dental pulp cells was investigated. For this study a hypothesis was made, that this molecules take part in the dental pulp inflammatory process through the cyclooxygenase-2 synthesis in human pulp cells. To evaluate this hypothesis the effect of LPS and LTA was studied in primary cultures of human pulp cells. Finally, the pathways and molecules involved in COX-2 expression were characterized. In human dental pulp cells treated with LTA or LPS, it was detected that these molecules promote COX-2 expression. On the other hand, it was founded that the expression of COX-2 in human pulp cells was blocked by treatments with SB203580, PD98059, and Estaurosporine. The results of the present study demonstrate that treatments with LTA and LPS promote COX-2 expression in events related with protein kinase p38, ERK and PKC. The findings of this investigation are important because COX-2 is related to the synthesis of PGE2, which leads to a chronic inflammatory process. Finally, the nature of a mediator implied in the pulpitis process was characterized.
Keywords: LTA; lypoteichoic acid; LPS; lipopolysaccharide; COX-2; cyclooxygenase-2; PGE2; prostaglandin E2; ERK; extracellular signal regulated protein kinases; PKC; protein kinases C; JNK; C-Jun NH2-terminal kinase
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