Services on Demand
Journal
Article
text new page (beta)
Spanish (pdf)
Article in xml format
Article references
Automatic translation
Send this article by e-mailIndicators
-
Cited by SciELO -
Access statistics
Related links
-
Similars in
SciELO
Share
Permalink
Revista odontológica mexicana
Print version ISSN 1870-199X
Rev. Odont. Mex vol.10 n.3 Ciudad de México Sep. 2006
https://doi.org/10.22201/fo.1870199xp.2006.10.3.15899
Trabajos originales
Ocurrencia de bacilos Gram negativos no fastidiosos y levaduras en la microbiota subgingival de pacientes chilenos con periodontitis
Occurrence of non fastidious Gram negative rods and yeasts in the subgingival microbiota of Chilean periodontitis patients
*Profesora Asistente, Departamento de Patología, Área de Microbiología, Facultad de Odontología, Universidad de Chile.
§Profesor Asociado, Departamento de Odontología Conservadora, Área de Periodoncia Facultad de Odontología, Universidad de Chile.
Antecedentes y objetivos
Grupos definidos de bacterias patógenas presentes en la microbiota subgingival se han asociado con periodontitis; actualmente, la terapia periodontal convencional tiende a la supresión o erradicación de patógenos periodontales específicos. En algunos pacientes se han podido observar casos de mala respuesta o de refractariedad a la terapia periodontal. Autores han asociado este hecho, en parte, a la presencia de patógenos inusuales en el saco periodontal, razón por la cual, el objetivo de este estudio fue determinar la ocurrencia de bacilos Gram negativos no fastidiosos y levaduras en la microbiota subgingival de un grupo de pacientes chilenos con periodontitis (crónica y agresiva).
Material y métodos
Se tomaron muestras de placa subgingival a 75 pacientes con periodontitis (crónica o agresiva), utilizando conos de papel, los que fueron depositados en RTF frío (4°C) y transportados al laboratorio para su procesamiento.
Resultados
Se determinó que un 22.6% de pacientes presentaba bacilos Gram negativos no fastidiosos y un 9.3% levaduras, el 64.8% de los aislados se identificó como Alcaligenes faecalis, el 17.6% Escherichia coli y el 17.6% restante fue Pseudomonas aeruginosa.
Palabras clave: Periodontitis; bacilos Gram negativos no fastidiosos; levaduras; microbiota subgingival
Background and aim
Groups of defined bacteria in the subgingival microbiota have been associated with periodontitis. Therefore, periodontal therapy is directed toward the suppression or elimination of particular periodontal pathogens. Some patients are refractory or poorly responsive to periodontal therapy. Some authors have related them with the presence of unusual pathogens in the subgingival microbiota. Thus, the aim of this work was to study the prevalence of non fastidious Gram negative rods and yeasts in the subgingival microbiota of a group of Chilean aggressive and chronic periodontitis patients.
Material and methods
Subgingival plaque samples from of 75 periodontitis patients (chronic or aggressive) were obtained with paper points and deposited in cold RTF (4°C) to be analyzed immediately.
Results
22.6% of patients harbored non fastidious Gram negative rods, and yeasts a 9.3% of them. In the first group 64.8% of isolates was Alcaligenes faecalis, 17.6% Escherichia coli and the other 17.6% was Pseudomonas aeruginosa.
Conclusions
No significant difference was observed between both groups of periodontitis patients (p > 0.05).
Key words: Periodontitis; non fastidious Gram negative rods; yeasts; subgingival microbiota
AGRADECIMIENTOS
Queremos expresar nuestros sinceros agradecimientos a la Facultad de Odontología de la Universidad de Chile y a la empresa Dentaid S.A. por el cons-tante apoyo en la implementación y manutención del Laboratorio de Microbiología.
Financiamiento: Proyecto PRI/ODO 00/13 Facultad de Odontología, Universidad de Chile. Laboratorios DENTAID S.A.
Referencias
1. Korman KS, Page RC, Tonette MS. The host response to the microbial challenge in periodontitis: assembling the player. Review Periodontology 2000 1997; 14: 33-53. [ Links ]
2. Slots J, Genco RJ. Black pigmented bacteroides species, cap-nocytophaga and Actinobacillus actinomycetemcomitans in human periodontal disease: virulence factors in colonization, survival, and tissue destruction. Journal of Dental Research 1984; 63: 412-421. [ Links ]
3. Haffajee AD, Socransky SS. Microbial etiological agents of destructive periodontal diseases. Periodontol 2000 1994; 5:78-111. [ Links ]
4. Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL Jr. Microbial complexes in subgingival plaque. J Clin Periodontol 1998; 25: 134-144. [ Links ]
5. Moore WEC, Moore LVH. Periodontal microbiota in different clinical conditions. Periodontol 2000 1994; 5: 66-77. [ Links ]
6. Slots J, Rams TE, Feik D, Taveras HD, Gillespie GM. Subgingival microflora of advanced periodontitis in the Dominican Republic. J Periodontol 1991; 62: 543-547. [ Links ]
7. Ali RW, Bakken V, NIlsen R, Skaug N. Comparative detection frequency of six putative periodontal pathogens in Sudanese and Norwegian chronic periodontitis patients. J Periodontol 1994; 65: 1046-1052. [ Links ]
8. Ali RW, Velcescu C, Jivanescu MC, Lofthus B, Skaug N. Prevalence of 6 putative periodontal pathogens in subgingival plaque samples from Romanian chronic periodontitis patients. J Clin Periodontol 1996; 23: 133-139. [ Links ]
9. López N, Mellado JC, Leighton GX. Occurrence of certain bacterial species and morphotypes in juvenile periodontitis in Chile. J Periodontol 1995; 66: 559-567. [ Links ]
10. Gajardo M, Silva N, Gómez L, León R, Parra B, Contreras A, Gamonal J. Prevalence of periodontopathic bacteria in aggressive periodontitis patients in a Chilean population. J Periodontol 2005; 76: 289-294. [ Links ]
11. Slots J, Rams TE. Systemic and topical antimicrobial therapy in periodontitis. Periodontol 2000 1996; 10: 5-159. [ Links ]
12. Socransky SS, Haffajee AD. Dental biofilms: difficult therapeutic targets. Periodontol 2000 2002; 28: 12-55. [ Links ]
13. Muller HP, Heinecke A, Borneff M, Kiencke C, Knopf A, Pohl S. Eradication of Actinobacillus actinomycetemcomitans from the oral cavity in adult periodontitis. J Periodontal Res 1998; 33: 49-58. [ Links ]
14. Baker PJ, Evans RT, Slots J, Genco RJ. Antibiotic susceptibility of anaerobic bacteria from the human oral cavity. J Den Res 1985; 64: 1233-1244. [ Links ]
15. Valdés MV, Lobbins PM. Beta-lactamase producing bacteria in the human oral cavity. J Oral Pathol 1982; 11: 58-63. [ Links ]
16. Slots J. Systemic in periodontics (Am Acad Periodontol position paper). J Periodontol 1996; 67: 831-838. [ Links ]
17. Slots J. Primer for antimicrobial periodontal therapy. J Periodontal Res 2000; 35: 108-114. [ Links ]
18. Haffajee AD, Socransky SS, Dzink JL. Clinical and microbiological features of subjects with intractable destructive periodontal disease. J Dent Res 1987; 66 (abstract 1868). [ Links ]
19. Slots J, Rams TE, Litsgarten MA. Yeasts, enteric rods and pseudomonas in the subgingival flora of severe adult periodontitis. Oral Microbiol Immunol 1988; 3: 47-52. [ Links ]
20. Campos CM, Zelante F. Contribuicao ao estudo da microbiota bucal humana. Ocorrencia de enterobacterias na saliva, lingua e placa dental. Rev Fac Odontol Sao Paulo 1978; 16: 77-86. [ Links ]
21. Sedgley CM, Samaranayake LP. The oral prevalence of aerobic and facultatively anaerobic gram-negative rods and yeasts in Hong-Kong Chinese. Arch Oral Biol 1994; 39: 459-466. [ Links ]
22. Sedgley CM, Chu CS, Lo ECM, Samaranayake LP. The oral prevalence of aerobic and facultatively anaerobic gram-negative rods and yeasts in semi-recluse human vegetarians. Arch Oral Biol 1996; 41: 307-309. [ Links ]
23. Sedgley CM, Samaranayake L, Chan JCY, Wey SHY. A 4 year longitudinal study of the oral prevalence of enteric gram-negative rods and yeasts in Chinese children. Oral Microbiol Immunol 1997; 12: 183-188. [ Links ]
24. Slots J, Feik D, Rams TE. Age and sex relationships of superinfecting microorganisms in periodontitis patients. Oral Microbiol Immunol 1990; 5: 305-308. [ Links ]
25. Barbosa FCB, Mayer MPA, Saba-Chubjfie, Cai S. Subgingival occurrence and antimicrobial susceptibility of enteric rods and pseudomonas from Brazilian periodontitis patients. Oral Microbiol Immunol 2001; 16: 306-310. [ Links ]
26. Loesche WJ, Hockett RM, Syed SA. The predominant cultivable flora of tooth surface plaque removal from institutionalized subjects. Arch Oral Biol 1972; 17: 1311-1325. [ Links ]
27. Slots J. Rapid identification of important periodontal microorga-nisms by cultivation. Oral Microbiol Immunol 1986; 1(1): 48-57. [ Links ]
28. Reynaud AH, Nygaard-Ostby B, Boygard G-K, Eribe E R, Olsen I, Gjermo P. Yeasts in periodontal pockets. J Clin Periodontol 2000; 28: 860-864. [ Links ]
29. Dahlén G, Wikstrom M. Occurrence of enteric rods, staphylococci and candida in subgingival samples. Oral Microbiol Immuno 1995; 10: 42-46. [ Links ]
30. Helovuo H, Hakkarainen K, Paunio K. Changes in the prevalence of subgingival enteric rods, staphylococci and yeasts after treatment with penicillin and erythromycin. Oral Microbiol Immunol 1993; 8: 75-79. [ Links ]
31. Rams TE, Babalola OO, Slots J. Subgingival occurrence of enteric rods, yeasts and staphylococci after systemic doxycycline therapy. Oral Microbiology & Immunology 1990;5: 166-168. [ Links ]
32. Listgarten MA, Lai CH, Young V. Microbial composition and pattern of antibiotic resistance in subgingival microbial samples from patients with refractory periodontitis. Journal of Periodontology 1993; 64: 155-161. [ Links ]
33. Aucken HM, Pitt TL. Antibiotic resistance and putative virulence factors of Serratia marcescens with respect to O and K serotypes. J Med Microbiol 1998; 47: 1105-1113. [ Links ]
34. Hejazi A, Falkkiner FR. Serratia marcescens. J Med Microbiol 1997; 46: 903-912. [ Links ]
35. Livrelli V, De Chaamp SC, di Martino P, Darfeville- Michaud A, Forestier C et al. Adhesive properties and antibiotic resistance of Klebsiella, enterobacter and serratia clinical isolates involved in nosocomial infections. J Clin Microbiol 1996; 34: 1963-1969. [ Links ]
36. Slots J, Feik D, Rams TE. Prevalence and antimicrobial susceptibility of enterobacteriaceae, Pseudomonadaceae and acinetobacter in human periodontitis. Oral Microbiol Immunol 1990; 5:149-154. [ Links ]
37. Pollack M, Anderson SE Jr. Toxicity of Pseudomonas aeruginosa exotoxin A for human macrophages. Infect Immun 1978; 19: 1092-1096. [ Links ]
38. Issekutz TB, Stoltz JM. Suppression of lymphocyte proliferation by Pseudomonas aeruginosa: mediation by Pseudomonas-activated suppressor monocytes. Infect Immun 1985;832-838. [ Links ]
39. Schultz DR, Miller KD. Elastase of Pseudomonas aeruginosa: inactivation of complement components and complement-derived chemotactic and phagocytic factors. Infect Immun 1974; 10: 128-135. [ Links ]
Este es un artículo publicado en acceso abierto bajo una licencia Creative Commons
