versión On-line ISSN 1870-0462
Trop. subtrop. agroecosyt vol.14 no.2 Mérida may./ago. 2011
Chemical composition and in vitro gas production of some legume browse species in subtropical areas of Mexico
Composición química y producción de gas in vitro de vainas de leguminosas arbóreas en areas subtropicales de México
C. A. Garcia-Montes de Ocaa, M. Gonzalez-Ronquilloa*, A. Z. M. Salemb,d, J. Romero-Bernala, J. F. Pedrazaa and J. G. Estradac
a Departamento de Nutricion Animal. Facultad de Medicina Veterinaria y Zootecnia. *Corresponding author E-mail adress: email@example.com
b Centro Universitario UAEM-Temascaltepec, km 67.5 Carr. Toluca-Tejupilco, Estado de Mexico, C.P. 51300, Mexico.
c Instituto en Ciencias Agropecuarias y Rurales. Universidad Autonoma del Estado de Mexico.
d Departament of Animal Production, Faculty of Agriculture (El-Shatby), Alexandria University, Egypt.
Submitted October 13, 2010
Accepted December 02, 2010
Revised received January 05, 2011
The objective of the present study was to determine the chemical composition and in vitro gas production of different legume and wild arboreal pods. Seven seeds of legume browse species, Mexican calabash (Crescentia alata), esculent leadtree (Leucaena esculenta), guamuchil (Phitecellobium dulce), bastard cedar (Guazuma ulmifolia), needle bush (Acacia farnesiana), mimosa (Mimosa sp.) and elephant ear tree (Enterolobium cyclocarpum). Were evaluated for their chemical composition (g/kg DM) and in vitro gas production pattern. Crude Protein was higher for L. esculenta (220) and lower for G. ulmifolia (70). Neutral and acid detergent fiber were higher for G. ulmifolia (687 and 554) and lower for A. farnesiana (267 and 176). Lignin was higher for Mimosa sp. (219) and lower for P. dulce (81). Total gas production (ml gas/g DM) of P. dulce (187) and E. cyclocarpum (164) were higher (P<0.001) than Mimosa sp. The lowest values were for C. alata (108), G. ulmifolia (102), L. esculenta (99) and A. farnesiana (90). The nutritional characteristics of L. esculenta and A. farnesiana might be used as supplements in ruminant diets, due to their major content in CP and in vitro digestibility, representing an alternative protein supplement during dry season.
Key words: Acacia farnesiana; Enterolobium cyclocarpum; Guazuma ulmifolia; Crescentia alata; Leucaena esculenta; Mimosa sp; Phitecellobium dulce; in vitro gas production; leguminous.
El objetivo del presente estudio fue determinar la composición química y la producción de gas in vitro de diferentes vainas de leguminosas arbóreas. Siete especies de leguminosas arbóreas fueron estudiadas: Cuatecomate (Crescentia alata), Guaje (Leucaena esculenta), Guamuchil (Phitecellobium dulce), Guazuma (Guazuma ulmifolia), Huizache (Acacia farnesiana), Mimosa (Mimosa sp.) y Parota (Enterolobium cyclocarpum). Fueron evaluadas en su composición química (g/kg MS) y producción de gas in vitro. La proteína cruda fue superior para L. esculenta (220) y menor para G. ulmifolia (70). El contenido de fibra neutro detergente y ácido detergente fueron superiores para G. ulmifolia (687 y 554) y menor para A. farnesiana (267 y 176). El contenido de lignina fue superior para Mimosa sp. (219) y menor para P. dulce (81). La producción total de gas (ml gas/g MS) para P. dulce (187) y E. cyclocarpum (164) fueron superiores (P<0.001) a Mimosa sp. Los menores valores fueron para C. alata (108), G. ulmifolia (102), L. esculenta (99) y A. farnesiana (90). Las características nutricionales de L. esculenta y A. farnesiana pueden ser utilizadas como suplementos en dietas para rumiantes, debido a su mayor contenido de PC y digestibilidad in vitro, representando una alternativa como suplemento proteico en las épocas secas.
Palabras clave: Acacia farnesiana; Enterolobium cyclocarpum; Guazuma ulmifolia; Crescentia alata; Leucaena esculenta; Mimosa sp; Phitecellobium dulce; producción de gas in vitro; leguminosas.
Mexico as an edaphically and topographic country and its climatic characteristics presents an important abundance of natural resources, mainly by the diversity of vegetal species. Nonetheless, in tropical and sub-tropical zones many of the native forages exhibit low quality; nevertheless, several fruits and seeds represent an alternative to supplement food to ruminants as protein sources, which might be utilized as a supplementing source in dry season.
Mexico has around 65% of arid and semiarid zones, among which one finds dry topic, in this zone it is common to observe native tree species which remain foliaged and fruited in dry season; they might be used as a strategy in the alimentation of the animals in this season (Alvarez et al., 2003). In addition to feed animals, the use of brows and shrubby species of natural vegetation can be as diverse, live fences, shade, medicinal, ornamental, etc. (Otarola, 1985; Negreros, 1993; Sosa, 2000). Likewise, they contribute to the sustainability of agricultural systems as they increase the recycling of nutrients, control erosion, improve the physical and biological conditions of the soil and are considered as elements of reforestation of the system (Moechiutti et al., 1995). Mexican browse species differ in their nutritional content depending on the time of the year and the zone where they grow and their utilization may be increased in the case of a multipurpose forage for the small producers, either for human consumption, livestock feeding, fuel, wood, etc; besides. Many producers use these species to build fences and provide shelter for the livestock.
There is very little information on their nutritional value and degradation at ruminal level for this last, the technique of in vitro gas production (Menke and Steingass, 1988; Theodorou et al., 1994) allows us to predict fermentation and degradation with very little material, which is easy to reproduce and has a high correlation with the in vivo methods (Getachew et al., 1998). The objective of the present study was to determine the chemical composition and in vitro gas production of different legume and wild arboreal pods.
MATERIALS AND METHODS
Sampling zones and recollection
Legume pods were collected from San Andres Nicolas Bravo, Municipality of Valle de Bravo, State of Mexico, Mexico; which is located at the southern edge of the occidental part of the State of Mexico, at 18º 45' 75" north latitude and 99º 27' 50" west longitude, at 1200 m above sea level, with sub-humid warm climate, mean annual temperature 34.8 ºC, rains in summer and an annual precipitation of 1.177mm3 (Schneider, 1999). The criterion used to select the plants was by means of questions to the smallholder of the region on which they used as a feed supplement for their animals in dry season. The pods were collected from September to December, being gathered ten plants (1.0 kg as fresh matter per plant) per species, which were classified as: Mexican calabash (Crescentia alata), esculent leadtree (Leucaena esculenta), guamuchil (Phitecellobium dulce), bastard cedar (Guazuma ulmifolia), needle bush (Acacia farnesiana), mimosa (Mimosa sp.) and elephant ear tree (Enterolobium cyclocarpum). Plant samples were mixed, and sub sampled (1.0kg DM) for latter analyses.
Samples were dried (60ºC, 24 h) and ground (cyclonic mill, 2 mm Ø), in order to determine their organic matter (OM) content, to do so the samples were incinerated at 550ºC for 3 h; the content of nitrogen was calculated (N) by means of the Kjeldalh (Büchi K-370) method using Cu as catalyst, and the content of crude protein (CP) multiplying N × 6.25 (AOAC, 1990, procedure 975.06), the content of neutral detergent fiber (NDF), acid detergent fiber (ADF) and lignin was carried out according to Van Soest et al. (1991) without correction by ashes.
In vitro gas production
The technique of in vitro gas production described by Theodorou et al. (1994) was performed, using two cannulated sheep in rumen as ruminal liquid donors. The animals were feed with alfalfa and oath hay (50:50) and 2% of a vitamin-mineral supplement (08:00 and 16:00 h), with water ad libitum. Ruminal liquor was collected from the two sheep, being filtered through four layers of cheese cloth gauze, and was gassed with CO2. The solution was prepared according to the technique described by Menke and Steingass (1988), 0.800 g of DM were threefold weighed in 125ml flasks and incubated with 90 ml of solution and 10ml of ruminal liquid; two flasks with barley hay and two without a sample (blanks) were included as correction factors. Gas production was registered at 3, 6, 9, 12, 24, 36 48 and 72 hours in four series of inoculations. Once the inoculation period concluded, the remainders of fermentation were recovered from the flasks and were dried (60ºC, 48h) in order to calculate the portion disappearance from DM (dDM; mg/g initial DM) and determine relative gas production (RGP, ml gas/g dDM) (Gonzalez Ronquillo et al., 1998). Once the results of ruminal degradation were calculated, the curve of gas production of the feed against time was established using the following equation proposed by Krishnamoorthy et al. (1991) GP = b (1-e-ct), where GP represents the accumulative gas production (ml/g DM), b is the total gas production (ml gas), c is the degradation rate in respect to time (h) and t is incubation time (h).
The mean of the three flasks of each sample and incubation series (four) were utilized as experimental unit; in order to study the differences between species and incubation series, they were contrasted by a variance analysis by incubation time, with the interaction of the incubation series by species, y = m + Plant speciei + incubation seriej + Plant specie*incubation seriek +eijk according to the procedures described by Steel et al. (1997). For all anayses, significance was declared at P = 0.05.
RESULTS AND DISCUSSION
The chemical composition of the forage pods is presented in Table 1, where we observe that P. dulce was the pod with the lowest DM. Alvarez et al. (2003) mention that the pod of E. cyclocarpum contains 90.5% of DM, being similar to the value obtained in the present study, in respect to most of the pods, Contreras et al. (1995) analyzing the addition of 4% of urea or sodium hydroxide present DM values for the pod of G. ulmifolia of 88%. As for the amount of OM, the pod of G. ulmifolia presents the highest content in relation to the pods of P. dulce, E. cyclocarpum, A. farnesiana, Mimosa sp. and L. esculenta, while the pod of C. alata presents the lowest content of OM. Alvarez et al. (2003), using diets for ewes, present values of OM similar to those obtained in the present study, likewise Francais (1991) mentions that the dry green fruit of G. ulmifolia contains 95% of OM, similar to the one found in the present study. Sosa et al. (2004) present values of OM for the pods of E. cyclocarpum of 92.5%, G. ulmifolia 89%, L. esculenta 88% and Mimosa sp. of 93%, which are similar to those obtained in the present study; depending on the region and season of the year the contents of DM and OM will vary (Sosa et al., 2004).
In relation to the amount of CP (g/kg DM), the pods of G. ulmifolia, Mimosa sp. and A. farnesiana contain the lowest concentration, while C. alata and L. esculenta, and present the highest contents. Alvarez et al. (2003) find CP values for the pod of E. cyclocarpum of 172, similar to the ones in the present study but above the findings of Velasco et al. (1996) of 132 for the seed and 150 for the pod, while Velazquez et al. (2005) present values for the pod of A. farnesiana of 111 of CP, being similar to that obtained in the present study. Even if we know that in several Mexican states there are numerous species with suitable characteristics to incorporate them into the diet of ruminants, their exploitation is limited (Benavides, 1993; Camero, 1995). Sosa et al. (2004) obtained CP values for the pods of E. cyclocarpum, L. esculenta, Mimosa sp., and P. dulce (19, 30, 12 and 18.5%, respectively), slightly above those in the present study with the exception of the pods of G. ulmifolia and P. dulce (7 and 14.5%, respectively).
The amounts of NDF and ADF in pods of G. ulmifolia, Mimosa sp. and L. esculenta is greater than C. alata, E. cyclocarpum, and P. dulce and the lowest was presented by A. farnesiana; in relation to the amount of Lignin, Mimosa sp. is higher in relation to G. ulmifolia and C. alata, Contreras et al. (1995) obtained values of lignin for the pod of G. ulcifolia of 18% treated with 4% of urea, being similar to those obtained in the present study, whilst the amount of ADF is lower than the one obtained in this study. Velazquez et al. (2005) present values of ADL for the pod of A. farnesiana at different levels of inclusion with an average of 12.3% being superior to that obtained in the present study; however the content of NDF and ADF is superior in relation to the present study. Sosa et al. (2004) present values of NDF and ADF for the pod of G. ulmifolia similar to those obtained in the present study, for the pods of E. cyclocarpum and Leucaena esculenta the values of the present study are below those obtained by said authors.
In vitro gas production
The dDM (mg/gDM) (Table 2) presents differences between species (P<0.001), being higher for the pods of A. farnesiana, G. ulmifolia and L. esculenta, in relation to the pods of E. cyclocarpum, P. dulce (375±36); on the contrary, RGP (ml gas/g dDM) was superior for the pod of P. dulce followed by E. cyclocarpum and Mimosa sp., in relation to the pods of C. alata, G. ulmifolia, L. esculenta and A. farnesiana. This shows an inverse relation between dDM and RGP, which would make us think that the lower the RGP the higher degradability, nonetheless some other anti-nutritional factors of these plants have to be considered, such as the content of tannins (Sosa et al., 2004). Macias and Garcia (2004) present in vitro digestibility values of 25% for the pod of G. ulmifolia being lower than those of the present study; this may be due to the fact that said authors found a higher percentage of NDF in this species, which decrease its digestibility. Contreras et al. (1995) show digestibility values of 48.5% for G. ulmifolia, being lower than the results obtained in the present study. On the contrary, Ortiz et al. (1989) find a digestibility value of 69.5% for the pod of E. cyclocarpum, which is superior to the obtained in the present study, these results show there is variability of the results found in the different studies, mainly due to the state of ripeness of the plants, which makes us suggest that the obtained results should be taken cautiously and extrapolated them to every zone of study and season of the year in order to provide a better recommendation to the producers, nevertheless the toxic and anti-nutritional factors presented by some of these species must be considered (Sosa et al., 2004).
Piri et al. (2007), using A. farnesiana and L. esculenta silage with maize (50:50) to fed sheep, find higher intake and digestibility of protein for L. esculenta, being this the best option to use it; our results present a higher content of protein in relation to A. farnesiana, even though they present similar dDM and RGP, we might use both A. farnesiana and L. esculenta as supplementation sources; however, Piri et al. (2007) recommend the use of A. farnesiana as a source of supplementation, but they consider that L. esculenta presents a lower content of CP, thereby the animals present lower intake of CP, we consider both are alternatives as supplements, being superior A. farnesiana, but we have to bear in mind that leguminous pods are an alternative where there is not another source of alimentation. In this case Saha et al. (2008) supplemented goats with L. esculenta and P. dulce and find protein values of 23 and 25% respectively, for the case of L. esculenta our values in CP and digestibility are similar to Saha et al. (2008), not so for P. dulce which are inferior, possibly due to the fact that we dehydrated the whole pod with seeds, which increases the content of NDF and OM but decreases its content of CP and digestibility, unlike Saha et al. (2008) where goats could select the pulp and seeds, and with it increase their intake of CP and its digestibility.
In figure 3, the results of total gas production (ml gas/ gDM) are shown on an hourly basis, having the highest production by P. dulce, at 12 hours, followed by E. cyclocarpum, and A. farnesiana, which were superior (P<0.05) to the rest.
The results of total gas production (ml gas/g DM) expressed by the hour in function of incubation time are shown in table 3, we observe that at the 3rd hour (H3) there are no significant differences for any of the analyzed legume pods; while at H6 there is a higher production of gas for P. dulce, followed by E. cyclocarpum and G. ulmifolia, being different (P<0.05) to the rest of the pods analyzed in the present study. At H9 we observe P. dulce and E. cyclocarpum present the highest gas productions, being different (P<0.05) from the rest of pods; while at H12, the highest production (P<0.05) was for P. dulce, followed by E. cyclocarpum and A. farnesiana. The highest production of gas was for P. dulce and E. cyclocarpum, being different from the rest of the pods used in the present study. Since leucaena has a lower effective degradability and slower degradation speed, Delgado et al. (2001) it might be better utilized as it allows part of the protein to pass towards the lower parts of the tract to be used by the animal; it seems as if with leucaena protein is more efficiently used (Ørskov, 1992). In figure 3 we observe an initial phase which is of slow or null production of gas, as it is seen at the 3rd hour, during this phase the hydration and colonization of the insoluble substrate by the ruminal microorganisms takes place (Cheng et al., 1980). When the substrate is saturated with microorganisms and enzymes, it enters into a phase where the most degradable part of the potentially soluble substrate is firstly degraded, as it occurs in the 9, 12, and 24 hours in the present study, and the least digestible substrate needs more time to be degraded; when finally the substrate is degraded the production begins to decrease, as it is observed as of the 36 until the 72 hour (Beuvink et al., 1993). Because of this the beginning of the fermentation of microbial population becomes a limiting factor, and by the end of fermentation it is the availability of fermentable substrate what limits the rate of gas production (Schofield et al., 1994).
The nutritional characteristics of some species of arboreal pods, such as L. esculenta and A. farnesiana allow them to be used as a complement in the diet of ruminants because of their higher content of protein and in vitro gas production, representing an option as a protein supplement in dry season. It is necessary however to broaden the knowledge on the identification of other species with potential to feed ruminants, their nutritional value and anti-nutritional values, conditions of handling and use by the producers for the benefit of the animals.
Mr. Garcia, and Mr. Romero, M in Sc. Vet., were funded by a CONACyT grant. The authors are grateful for the cooperation of Mr. Garcia Alvarez. and Ibarra Zimbron, M in Sc., for the sample gathering.
Alvarez, M.G., Melgajero, V.L., Castañeda, N.Y. 2003. Weight gain, feed conversion and efficiency in sheep fed with Enterolobium cyclocarpum tree (Enterolobium cyclocarpum) fruit (seed and pod) and poultry manure. Veterinaria Mexico 34:39-46. [ Links ]
AOAC, 1990. Association of Official Analytical Chemists, Official Methods of Analysis, 15th edition. AOAC, International Arlington, VA, USA. [ Links ]
Benavides, J.E. 1993. Fodder trees in Central America. In: II American Seminar and Caribbean Center on Agroforestry in small ruminants. San Jose, Costa Rica, 1993. 1-33. [ Links ]
Beuvink, J.M.W., and Kogut, J. 1993. Modeling gas production kinetics of grass silages incubated with buffered ruminal fluid. Journal of Animal Science. 71: 1041-1046. [ Links ]
Camero, R.A. 1995. Developed experiences for CATIE in the usage of forage as Eritrina sp. y Gliricidia sepium in beef cattle and dairy. Agroforesteria en las Americas 2: 9-13. [ Links ]
Cheng, K.T., Fray, J.P., Howarth, R.E., Costerton, J.W. 1980. Sequence of events in the digestion of fresh legume leaves by the rumen bacteria. Applied Environ Microbial 40: 613-625. [ Links ]
Contreras, L.D., Gutierrez, C.H.L., Ramirez, C.T., Lopez, R.A. 1995. Improvement nutritive value for dry seeds of Guazuma (Guazuma ulcifolia) with urea or sodium hydroxide. Archivos de Zootecnia. 44: 49-53. [ Links ]
Delgado, D.C., O, L.A., Chongo, B., Galindo, J., Obregon, Y., Aldama, I.A. 2001. In Situ ruminal degradation in four tropical shrubs trees: Leucaena leucocephala, Enterolobium cyclocarpum, Sapindus saponaria y Gliricidia sepium. Revista Cubana de Ciencia Agricola 35: 141-145. [ Links ]
Francais, J.K. 1991. Guazuma ulmifolia Lam. Guacima. SO-ITF-SM-47. New Orleans, LA: U.S. Department of Agriculture, Forest Service, Southern Forest Experiment Station. p 5 [ Links ]
Getachew, G., Blümel, M., Makkar, H.P.S., Becker, K. 1998. In vitro gas measuring techniques for assesment of nutritional quality of feeds: a review. Animal Feed Science and Technology. 72: 261-281. [ Links ]
Gonzalez Ronquillo, M., Fondevila, M., Barrios., U.A., Newman, Y., 1998. In vitro gas production from buffel grass (Cenchrus ciliaris L.) fermentation in relation to the cutting interval, the level of nitrogen fertilization and the season of growth. Animal Feed Science and Technology. 72: 19-35. [ Links ]
Krishnamoorthy, U., Soller, H., Steingass, H., Menke, K.H. 1995. Energy and protein evaluation of tropical feedstuffs for whole tract and ruminal digestion by chemical analyses and rumen inoculum studies in vitro. Animal Feed Science and Technology. 52: 177-188. [ Links ]
Macias, M., Garcia, A. 2004. Note on the chemical composition of tree foliage from western mountains of Cuba. Revista computadorizada de produccion porcina 11: 41-45. [ Links ]
Menke, K.H., Steingass, H. 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development 28: 7-45. [ Links ]
Moechiutti, S., Torres, M., Oviedo, F., Vallejo, M., Benavides, J. 1995. Goat milk supplementation with different levels of clavelon (Hibiscus rosa-sinensis). Agroferesteria en las Americas 2: 12-18. [ Links ]
Negreros, P.N. 1993. Multipurpose trees for agroforestry in Quintana Roo state. Reporte tecnico interno del programa ICRAF en Mexico, 58. [ Links ]
Otarola, A. 1985. Life fences with black wood. Agroforestry practice in different places during the dry season. Agroforesteria en las Americas 2: 24-30. [ Links ]
Ortiz, M.A., Gonzalez, J.M., Bressani, R. 1989. Feed value on Enterolobium cyclocarpum fruits for calves. Turrialba, Costa Rica, Centro de Agronomia Tropical de Investigacion y Enseñanza Turrialba. 39: 209-214. [ Links ]
Ørskov, E.R. 1992. Protein Nutrition in Ruminants. Academy Press Limited U.K., London. 175 p. [ Links ]
Palma, J.M. 2006. Silvopastoral system in the dry tropics of Mexico. Archivos Latinoamericanos de Produccion Animal 14: 95-104. [ Links ]
Piri, M.S., Ngongoni, N.T., Maasdorp, B.V., Titterton, M., Mupangwa, J.F., Sebata, A. 2007. Ensling characteristics and feeding value of silage made from Browne tree legume-maize mixtures. Tropical and Subtropical Agroecosystems 7: 149-156. [ Links ]
Saha, H.M., Kahindi, R.K., Muinga, R.W. 2008. Evaluation of manure from goats fed panicum basal diet and supplemented with madras thorn, Leucaena esculenta or gliciridia. Tropical and Subtropical Agroecosystems 8: 251-257. [ Links ]
Schneider, L.M. 1999. Municipal monographic. Malinalco. Instituto Mexiquense de Cultura. Toluca, Mexico. [ Links ]
Schofield, P., Pitt., R.E, Pell, A.N. 1994. Kinetics of fiber digestion from in vitro gas production. Journal Animal Science. 72: 2980-2981. [ Links ]
Sosa, R.E., Perez, R.D., Ortega, R.L., Zapata, B.G. 2004. Tropical trees and shrubs forage potential for sheep feeding. Tecnica Pecuaria en Mexico. 42: 129-144. [ Links ]
Sosa, R.E., Sansores, L.L., Zapata, B.G., Ortega, R.L. 2000. Botanical composition and nutritive value of cattle diet in secondary vegetation in Quintana Roo. Tecnica Pecuaria Mexico. 38: 105-117. [ Links ]
Steel, R.D.G., Torrie, J.H., Dickey, D.A. 1997. Principles and Procedures of Statistics: A Biometrical Approach. 3rd ed. McGraw-Hill Book Co, Inc, New York. [ Links ]
Theodorou, M.K., Williams, B.A., Dhanoa, M.S., Mc Allan, A.B., France, J. 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology. 48: 185-197. [ Links ]
Van Soest, P.J., Robertson, J.B., Lewis, B.A. 1991. Methods for dietary fiber, neutral detergent fiber, and non starch polysaccharides in relation to animal nutrition. Journal of Dairy Science. 74: 3583-3597. [ Links ]
Velasco, A.O., Melgarejo, V., Velasco, N.F. 1996. Fed convertion, weight gain and dressing percentage of steers fed different proportions of the fruit of Enterolobium cyclocarpum. Proceedings of XX National Congres of Buiatrics, August 14-17; Acapulco (Guerrero) Mexico. Veterinary Medical Association of Cattle Specialists A.C. 301-304. [ Links ]
Velazquez, A.J, Perezgrovas, G.R., Velasco, Z.M.E., Zaragoza, M.L., Rodriguez, G.G. 2005. Assesement of quebracho (Acacia farnesiana) pods utilization in wool-sheep feeding. Archivos de Zootecnia 54: 535-549. [ Links ]