Biotecnología

 

Evaluación de la inhibición de Candida intermedia por efecto de sobrenadantes de bioprotectores y su efecto sinérgico

 

Evaluation of inhibition of the Candida intermedia due to the effect of supernatants from bioprotectors and their synergistic effect

 

A. Henao-Ardila¹, M.X Quintanilla-Carvajal¹, B.F Klotz-Ceberio² y J.A Serna-Jiménez¹*

 

¹ Universidad de La Sabana, Facultad de Ingeniería de Producción Agroindustrial, Km. 7, Autopista Norte de Bogotá. Chía, Colombia. *Autora para la correspondencia. E-mail: andreasernajimenez@hotmail.com

² Instituto Alpina de Investigación, Alpina Corporativo S.A., Km 3 Vía Briceño- Sopó, Sopó, Cundinamarca, Colombia.

 

Received March 23, 2014
Accepted May 28, 2015

 

Resumen

En este trabajo se evaluó el efecto inhibitorio de los sobrenadantes de tres bioprotectores comerciales (HOLDBAC YM-C® 100DCU, MicroGARD 200® y Lyofast FPR2®), con el fin de establecer el bioprotector con mayor efecto inhibitorio sobre la levadura alterante de alimentos Candida intermedia. De igual forma, se estudió el efecto sinérgico que tienen los bioprotectores, por medio de un diseño experimental de mezcla optimizada, para establecer el porcentaje óptimo de mezcla que maximice la inhibición de crecimiento de la Candida intermedia a una concentración de 10 exp 5 UFC/mL. El mayor efecto inhibitorio se obtuvo empleando los sobrenadantes del bioprotector MicroGARD 200®, reduciendo la carga microbiana en 3.2 ciclos logarítmicos, mientras que HOLDBAC YM-C® y Lyofast FPR2® redujeron la carga microbiana en 1.7 y 1.2 ciclos logarítmicos respectivamente. Se determinó que existe un efecto sinérgico entre los sobrenadantes de los bioprotectores y la mezcla óptima que maximiza la inhibición de la Candida intermedia contenía 23.7% de HOLDBAC y 76.3% de MicroGARD, reduciendo la carga microbiana en 3.4 ciclos logarítmicos.

Palabras clave: bioprotectores, sobrenadantes, Candida intermedia, optimización de mezcla, inhibición.

 

Abstract

In this work, the inhibitory effect of the supernatants of three commercial bioprotectors (HOLDBAC YM-C® 100DCU, MicroGARD 200® y Lyofasr FPR2®) was evaluated sn order to establish the most effective bioprotector against the food spoilage yeast Candidal intermedia. Additional, the synergistic effect of the bioprotectors was studied through an experimental design of optimized mixture to determine the optimum mixing ratio that maximizes inhibition of Candida intermedia at a concentration of 10 exp 5 CFU/mL. The highest inhibition was obtained using the supernatants of MicroGARD 200®, reducing the microbial load in 3.2 logarithmic cycles, while HOLDBAC YM-C® and Lyofast FPR2® reduced the microbial load in 1.7 and 1.2 logarithmic cycles respectively. It was determined that there is a synergistic effect between the supernatants of the bioprotectors and that the optimum mixing of bioprotectors that maximized inhibition of Candida intermediate contained 23.7 % of HOLDBAC and 76.3 % of MicroGARD, reducing the microbial load in 3.4 logarithmic cycles.

Key words: bioprotectors, supernatants, Candida intermedia, optimum mixing ratio, maximum inhibition.

 

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