Biotecnología

 

Producción de metano utilizando residuos cunícolas

 

Methane production using rabbit residues

 

O. Teniza-García¹'², M.M. Solís-Oba*, M.E. Pérez-López³, J.M. González-Prieto⁴ y R. Valencia-Vázquez⁵

 

¹ IPN, Centro de Investigación en Biotecnología Aplicada, Carretera Tecuexcomac-Tepetitla Km 1.5, C.P. 90700, México.

² CECyTE Tlaxcala. Reforma No 10 Tlatempan, Tlaxcala C.P.90610, México. *Autora para la correspondencia. E-mail: myrobatlx@yahoo.com.mx, Tel. 012484870765, Fax 012484870762.

³ Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional, Sigma 119 Durango, C.P. 34220, México.

⁴ Centro de Biotecnología Genómica. Boulevard del Maestro s/n esq. Elías Piña, Cd. Reynosa, Tamaulipas, C.P. 88710, México.

⁵ Instituto Tecnológico de Durango. Boulevard Felipe Pescador 1830 Ote. Durango, Dgo. C.P. 3408, México.

 

Recibido 7 de Abril de 2014
Aceptado 31 de Mayo de 2015

 

Resumen

En las granjas cunícolas de Tlaxcala se obtienen dos residuos: una mezcla de aserrín, estiércol y orina de conejo (R₁) proveniente del criadero y las vísceras (R₂) proveniente del proceso de matanza. En este trabajo fue evaluada la digestión anaerobia de dichos residuos para definir las condiciones bajo las cuales se puedan utilizar como fuente de energía alternativa. La producción de metano fue valorada en cuatro etapas, la primera fue la digestión de R₁ y R₂ inoculados con estiércol de vaca (A), cerdo (B) y cabra (C), para seleccionar el par residuo-estiércol a utilizar. En la etapa dos fue considerado el tratamiento de mayor producción de metano de la etapa uno y se evaluó el ajuste de: pH a 7.2 y/o la relación C/N a 23/1; en la etapa tres se evaluó el impacto de dos valores de temperatura (ambiente y 37°C) y dos fuentes de carbono (paja de avena y aserrín), así como la adición de micronutrientes (etapa 4) sobre la producción de biogas. Se encontró que la digestión anaeróbica de las vísceras de conejo con 10% de estiércol de cabra generó la mayor producción de biogas con 71 % de metano; lo cual fue logrado al ajustar los parámetros de proceso (pH a 7.2, relación C/N a 23/1, temperatura de 37°C y adición de micronutrientes). El análisis estadístico mostró que la temperatura es el parámetro que tuvo el mayor efecto sobre la producción de metano, la adición de micronutrientes influyó en reducir el tiempos para obtener biogás con al menos 45% de metano (mínimo para ser considerado combustible), y la sustitución de paja de avena por aserrín no tuvo efecto significativo en la producción de biogas y metano.

Palabras clave: estiércol de conejo, vísceras, producción de metano, biogás, co-digestión.

 

Abstract

At the rabbit farms of Tlaxcala, Mexico two residues are produced: a mixture of ^rawdustt with rabbit: manure and urine (R₁) from the hatchery area, and the viscera (R₂) from the slaughter process. In this work, the anaerobic digestion (AD) process of such residues was evaluated to define the conditions at which they can be used to produce an alternative energy source. Methane content of the biogas produced was assessed in four stages. The first stage was the co-digestion of R₁ and R₂ inoculated with different manures: cow (A), pig (B) and goat (C), in order to select the residue-manure pair to be used. At stage two, the treatment which performed better at the previous stage was selected to evaluate the effect of pH adjustment to 7.2 and/or a C/N ratio to 23/1; during the third stage, it was evaluated the impact of internal temperature (ambient temperature and 37°C) and the addition of two carbon sources (oat straw and sawdust) on the methane content of the biogas produced; and finally, at stage four, the addition of micronutrients (step 4) over methane production was assessed. It was found that anaerobic digestion of rabbit entrails inoculated with 10% of goat manure generated the highest production of biogas with a 71% methane content; which was achieved by adjusting the process parameters (pH 7.2, C/N ratio to 23/1, internal temperature to 37°C and addition of micronutrients). Statistical analyses showed that temperature was the parameter that had the greatest effect on the methane content of the produced biogas; adding micronutrients reduced the lag-phase and helped to achieve 45% of methane in the biogas (minimal value required to be flammable); and, replacing sawdust by oat straw had no significant effect on the production of biogas or methane content.

Key words: rabbit manure, internal organs, methane production, biogas, co-digestion.

 

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Agradecimientos

El presente proyecto se llevó a cabo gracias al financiamiento otorgado por el Consejo Nacional de Ciencia y Tecnología (CONACyT), a la propuesta número 138741 de la convocatoria de Proyectos de Investigación, Desarrollo o de Innovación Tecnológica 2010. El primer autor agradece al CONACYT por la beca 175787 y al Instituto Politécnico Nacional por las becas PIFI de los proyectos SIP20110337, 20113440, 20120992 y 20130635.

 

Referencias

Abbassi, G.A., Brockmann, D., Trably, E., Dumas, C., Delgenes, J.P., Steyer, J.P. y Escudie, R. (2012). Total solids content drives high solid anaerobic digestion via mass transfer limitation. Bioresource Technology 111, 55-61.         [ Links ]

Adelard, L., Poulsen, T.G. y Rakotoniaina V. (2015). Biogas and methane yield in response to co-and separate digestion of biomass wastes. Waste Management and Research 33, 55-62.         [ Links ]

Alburquerque, J., De la Fuente, C., Ferrer-Costa, A., Carrasco, L., Cegarra, L., Abad, M., Bernal, M. (2012). Assessment of the fertilizer potential of digestates from farm and agroindustrial residues. Biomass and Bioenergy 40, 181-189.         [ Links ]

Balagurusamy, N. (2007). A preliminary study on molecular characterization of the Eubacteria in a thermophilic, poultry waste fed anaerobic digester. Revista Mexicana de Ingeniería Química 6, 237-242.         [ Links ]

Babaee, A., Shayegan, J. y Roshani, A. (2013). Anaerobic slurry co-digestion of poultry manure and straw: effect of organic loading and temperature. Journal of Environmental Health Sciences & Engineering 2013, 11-15        [ Links ]

Bayr, S., Rantanen, M., Kaparaju, P. y Rintala, J. (2012). Mesophilic and thermophilic anaerobic co-digestion of rendering plant and slaughterhouse wastes. Bioresource Technology 104, 28-36.         [ Links ]

Bekkering, J., Broekhuis, A.A. y Gemert, V. (2010). Optimisation of a green gas supply chain - A review. Bioresource Technology 101, 450-456.         [ Links ]

Budiyono, B., Widiasa, I.N., Johari, S. y Sunarso, S. (2010). Increasing Biogas Production Rate from Cattle Manure Using Rumen Fluid as Inoculums. International Journal of Basic and Applied Sciences 10, 68-75.         [ Links ]

Carrera, E.J.L., Guzmán, V. C.H., Ortiz, R. A., Desiga, O. O. y García, R.M.A. (2013). Thermodynamic one-zone model with relations for combustioín process for biogas fueled internal combustion engines. Revista Mexicana de Ingeniería Química 12, 649-660.         [ Links ]

Chandra, R., Takeuchi, H. y Hasegawa, T. (2012). Methane production from lignocellulosic agricultural crop wastes: A review in context to second generation of biofuel production. Renewable and Sustainable Energy Reviews 16, 1462-1476.         [ Links ]

Chen, Y., Cheng, J.J. y Creamer, K.S. (2008). Inhibition of anaerobic digestion process. A review. Bioresource Technology 99, 4044-4064.         [ Links ]

Cuetos, M.J., Gomez, X., Otero, M. y Moran A. (2010). Anaerobic digestion and co-digestion of slaughterhouse waste (SHW): influence of heat and pressure pre-treatment in biogas yield. Waste Manage 30, 1780-1789.         [ Links ]

Demirel, B. y Yenigun, O. (2002). The effects of change in volatile fatty acid (VFA) composition on methanogenic up flow filter reactor (UFAF) performance. Environmental Technology 23, 1179-87.         [ Links ]

Demirel, B. y Scherer, P. (2011). Trace element requirements of agricultural biogas digesters during biological conversion of renewable biomass to methane: Review. Biomass and Bioenergy 35, 992-998.         [ Links ]

Facchin, V., Cavinato, C., Fatone, F., Pavan P., Cecchi, F. y Bolzonella D. (2013). Effect of trace element supplementation on the mesophilic anaerobic digestion of food waste in batch trials: The influence of inoculum origin. Biochemical Engineering Journal 70, 71-77.         [ Links ]

Feng, H., Qu, G., Ning, P., Xiong, X., Jia, L., Shi, Y., Zhang, J. (2011). The Resource Utilization of Anaerobic Fermentation Residue. Procedia Environmental Science 11, 1092-1099.         [ Links ]

Ferreira-Rolón, A., Ramírez-Romero, G., Ramírez-Vives, F. (2014). Aumento de la actividad metanogénica en lodos granulares, precipitando calcio en el nejayote mediante el burbujeo de CO₂. Revista Mexicana de Ingeniería Química 13, 517-525.         [ Links ]

Flores, R., Muñoz-Ledo, R., Flores, B.B., Cano, K.I. (2008). Estimación de la generación de energía a partir de biomasa para proyectos del programa de mecanismo de desarrollo limpio. Revista Mexicana de Ingeniería Química 7, 35-39.         [ Links ]

Gan, J., Montaño, M., Fajardo, C., Meraz, M., Castilla P. (2013). Anaerobic co-treatment of leachates produced in a biodegradable urban solid waste composting plant in México city. Revista Mexicana de Ingeniería Química 12, 541-551.         [ Links ]

Gerardi, M.H. (2003). The Microbiology of Anaerobic Digesters. Wastewater Microbiology series. Wiley Interscience. United States of América.         [ Links ]

Gomez, T. F., Celis, L.B., Razo, F. E. y Alatriste, M.F. (2012). Chemical and enzymatic sequential pretreatment of oat straw for methane production. Bioresource Technology 116, 372-378.         [ Links ]

Guangqing, L., Zhang, R., El-Mashad, H.M. y Dong, R. (2009). Effect of feed to inoculum ratios on biogas yields of food and green wastes. Bioresource Technology 100, 5103-5108.         [ Links ]

Hassan, D. y Tandon, S.M. (1987). Response of a cattle dung methane fermentation to nickel. Biological Wastes 22, 261-268.         [ Links ]

Hill, D.T. y Holmberg R.D. (1988) Long Chain Volatile Fatty Acid Relationships in Anaerobic Digestion of Swine Waste. Biological Wastes 23, 195-215.         [ Links ]

Kim, J. y Kang, Ch, M. (2015). Increased anaerobic production of methane by co-digestion of sludge with microalgal biomass and food waste leachate. Bioresource Technology 189 409-412.         [ Links ]

Kuhad, R.C, Singh A. y Eriksson K.E. (1997). Microorganisms and enzymes involved in degradation of plant fiber cell walls. Journal of Advances in Biochemical Engineering and Biotechnology 57, 45-125.         [ Links ]

Lorenzo, A.Y. y Obaya, A.M.C. (2005). La digestion anaerobia. Aspectos teoíricos. Parte I. Red de Revistas Científicas de America Latina, el Caribe, España y Portugal 39, 35-48.         [ Links ]

Luste, S. y Luostarinen, S. (2010). Anaerobic co-digestion of meat-processing by-products and sewage sludge-Effect of hygienization and organic loading rate. Bioresource Technology 101, 2657-2664.         [ Links ]

Marañon, E., Castrillón, L., Quiroga, G., Fernández-Nava, Y., Gómez, L. y García, M.M. (2012). Co-digestion of cattle manure with food waste and sludge to increase biogas production. Waste Management 32, 1821-1825.         [ Links ]

Méndez-Contreras, J.M., Rendón-Sagardi, J.A., Ruiz-Espinoza, J.E., Alvarado-Lassman, A., Martínez-Delgadillo, S.A. (2009). Behavior of the mesophilic and thermophilic anaerobic digestion in the stabilization of municipal wastewater sludge (part 1). Revista Mexicana de Ingeniería Química 8, 283-290.         [ Links ]

Mshandete, A., Kivaisi, A., Rubindamayugi, M. y Mattiasson, B. (2004). Anaerobic batch co-digestion of sisal pulp and fish wastes. Bioresource Technology 95, 19-24.         [ Links ]

Navarro, A.F., Cegarra, J., Roig, A. y García, D. (1993). Relationships between organic matter and carbon contents of organic wastes. Bioresource Technology 44, 203-207.         [ Links ]

Passanha, P., Esteves, S., Kedia, G., Dinsdale, R., Guwy, A. 2013. Increasing polyhydroxyalkanoate (PHA) yields from Cupriavidus necator by using filtered digestate liquors. Bioresource Technology 147, 345-352.         [ Links ]

Patel, V.B., Patel, A.R., Patel M.C., y Madamwar D.B. (1993) Effect of metals on anaerobic digestion of water hyacinth-cattle dung. Applied Biochemistry and Biotechnology 43, 45-50.         [ Links ]

Rasi, S., Veijanen, A. y Rintala, J. (2007) Trace compounds of biogas from different biogas production plants. Energy 32, 1375-1380.         [ Links ]

Resch, C., Worl, A., Waltenberger, R., Braun, R. y Kirchmayr, R. (2011). Enhancement options for the utilisation of nitrogen rich animal by products in anaerobic digestion. Bioresource Technology 102, 2503-2510.         [ Links ]

Srivastava, R., Arango, M., Sharma, A.K. Cow dung extract: a medium for the growth of pseudomonads enhancing their efficiency as biofertilizer and biocontrol agent in rice. Indian Journal of Microbiology 50, 349-354.         [ Links ]

Terreros-Mecalco, J., Olmos-Dichara, A., Noyola-Robles, A., Ramírez-Vives, F., Monroy-Hermosillo, O. (2009). Digestión anaerobia de lodo primario y secundario en dos reactores UASB en serie. Revista Mexicana de Ingeniería Química 8, 153-161.         [ Links ]

Valladão, G.B.A., Freire, G.M.D. y Cammarota, C.M. (2007). Enzymatic pre-hydrolysis applied to the anaerobic treatment of effluents from poultry slaughterhouses. International Biodeterioration and Biodegradation 60, 219-225.         [ Links ]

Vigueras, C.S.E., Ramírez, V.F., Noyola, R.N. y Monroy, H.O. (2011). Efecto del pre tratamiento termo alcalino en la digestión anaerobia mesofílica y termofílica de lodos residuales secundarios. Revista Mexicana de Ingeniería Química 10, 247-255.         [ Links ]

Vigueras, C.S.E., Zafra, J.G., García, R.M., Martínez, T.M.A. y Pérez, V.J. (2013) Effect of various pretreatments on anaerobic biodegradability and quality microbiology of waste activated sludge. Revista Mexicana de Ingeniería Química 12, 293-301.         [ Links ]

Wang, L., Li, Y., Chen, P., Min, M., Chen, Y., Zhu, J., Ruan, R. 2010. Anaerobic digested dairy manure as a nutrient supplement for cultivation of oil-rich green microalgae Chlorella sp. Bioresource Technology 101, 2623-2628.         [ Links ]

Wilkie, A., Goto, M., Bordeaux, F.M. y Smith, P.H. (1986). Enhancement of anaerobic methanogenesis from napiergrass by addition of micronutrients. Biomass 11, 135-146.         [ Links ]

Wu, X., Yao, W., Zhu, J. y Miller, C. (2010) Biogas and CH4 productivity by co-digesting swine manure with three crop residues as an external carbon source. Bioresource Technology 101, 4042-4047.         [ Links ]