Biotecnología

 

Production of calcium- and iron-binding peptides by probiotic strains of Bacillus subtilis, B. clausii and B. coagulans GBI-30

 

Producción de péptidos fijadores de calcio y hierro por cepas probióticas de Bacillus subtilis, B. clausii y B. coagulans GBI-30

 

A.I. Reyes-Méndez, C. Figueroa-Hernández, G. Melgar-Lalanne, H. Hernández-Sánchez,* G. Dávila-Ortiz, and C. Jiménez-Martínez

 

Departamento de Graduados e Investigación en Alimentos, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala, Col. Sto. Tomás, CP 11340, México, D.F. México. *Corresponding author. E-mail: hhernan1955@yahoo.com Tel. +(52 55) 5729-6000

 

Recibido 4 de Noviembre 2013
Aceptado 24 de Enero de 2014

 

Abstract

Some strains of Bacillus subtilis, B. clausii and B. coagulans are commercially used as probiotic bacteria and their proteolytic activity is well known. The aim of this work was to evaluate their capacity to produce calcium- and iron-binding peptides when grown in culture media with different nitrogen sources. The fermentation media included broths containing tryptic soy (TS), casein peptone (CP), soy peptone (SP), and a crude phycobiliprotein extract (CPE). Cell-free supernatants (CFS) were prepared from the fermented media after 24, 48 and 72 h of fermentation and, tested for degree of hydrolysis (DH). Calcium- and iron-binding activities were determined as well. When the inocula were prepared in a medium designed for the production of alkaline proteases (AP), the degree of hydrolysis and the mineral-binding activity in the CFS were higher. The best rerults for calcium-binding activity and DH were obtained when B. subtilis and B. coagulans grew in a CPE-containing medium. Analogous results were found for trie iron-binding activity; nevertheless, this bioactivity was also high for B. clausii when grown in broths containing TS-, CP- and, SP.

Keywords: bioactive peptides, Bacillus subtilis, Bacillus clausii, Bacillus coagulans, calcium-binding, iron-binding, proteolysis, spirulina.

 

Resumen

Algunas cepas de Bacillus subtilis, B. clausii y B. coagulans se utilizan comercialmente como probioticos y su actividad proteolítica es de sobra conocida. El objetivo de este trabajo fue la evaluation de estos microorganismos debido a su capacidad para producir peptidos fijadores de calcio y hierro cuando son cultivados en medios con diferentes fuentes de nitrógeno. Los medios para la fermentación incluyeron caldos con soya y triptona (ST), peptona de caseína (PC), peptona de soya (PS) y, un extracto crudo de ficobiliproteína (ECF). Se obtuvieron sobrenadantes libres de celulas (SLC) a partir de los caldos de fermentation y se determino el grado de hidrólisis (GH) y la bioactividad fijadora para calcio y hierro. El GH y la actividad fijadora de minerales fue mayor cuando se emplearon inoculos preparados a partir de un medio disenado para la production de proteasas alcalinas (AP). El mejor resultado para el GH y la bioactividad fijadora de calcio se obtuvo con B. subtilis y B. coagulans cultivados en caldo con ECF como fuente de nitrógeno. Se observaron resultados similares para la actividad fijadora de hierro, sin embargo, tambien se obtuvieron buenos resultados con B. clausii cuando se utilizaron para su crecimiento los caldos ST, PC y PS.

Palabras clave: péptidos bioactivos, Bacillus subtilis, Bacillus clausii, Bacillus coagulans, fijadores de calcio, fijadores de hierro, proteólisis, spirulina.

 

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