Artículos regulares/Biotecnología

 

A statistically motivated choice of process parameters for the improvement of canthaxanthin production by Dietzia maris NIT-D (Accession number: HM151403)

 

Elección de parámetros de proceso mediante análisis estadístico para mejorar la producción de cantaxantina usando Dietzia maris NIT-D (Número de acceso: HM151403)

 

J. De Neve¹*, G. Goswami², A. Dutta^3,4, S. Chaudhuri² and D. Dutta²

 

¹ Department of Mathematical Modelling, Statistics and Bioinformatics, Ghent University. Coupure Links 653, 9000 Ghent, Belgium. Corresponding author. E-mail: JanR.DeNeve@UGent.be Tel. +32 (0) 9 264 59 38, Fax +32 (0) 9 264 62 20.

² Department of Biotechnology, National Institute of Technology, Durgapur, Ml. G. Avenue, Durgapur 713209, West Bengal, India.

³ Faculteit Industriële Ingenieurswetenschappen, KU Leuven, Campus Leuven (@Groep T), Andreas Vesaliusstraat 13, B-3000 Leuven, Belgium.

⁴ Departement Metaalkunde ein Toegepaste Matertaalkunde (MTM), KU Leuven, Kasteelpark Arenberg 44, B-3001 Heverlee-Leuven, Belgium.

 

Received: December 12, 2013.
Accepted: February 8, 2014.

 

Abstract

Dietzia maris NIT-D, a producer of canthaxanthin, is isolated during routine screening of pigment producing bacteria. The effects of process parameters, namely temperature, pH, shaker speed, percentage inoculum, medium volume, and concentration of glucose on the canthaxanthin production are studied by using response surface design methodology. The optimal conditions are temperature = 30°C, pH = 5.5), shaker speed = 125 rpm, inoculum = 1.9 %, volume = 50 mL, and glucose = 15 g L^-1, resulting in a canthaxanthin production of 152 mg L^-1, which is 25% higher than that of a recently reported study.

Keywords: Dietzia maris NIT-D, biomass, canthaxanthin, response surface methodology.

 

Resumen

Dietzia maris NIT-D una bacteria productora de cantaxantina, fue aislada durante una selección de cepas. Mediante un diseño de superficie de respuesta fueron estudiados los efectos de los parámetros del proceso para la producción de este pigmento: temperatura, pH, velocidad de agitación, porcentaje de inóculo, volumen del medio de cultivo y concentración de glucosa. Las condiciones óptimas fueron 30°C, pH de 5.9, 125 rpm, 1.9 % de inóculo, 50 mL de medio de cultivo y 15 g L^-1 de glucosa. Como resultado la producción de cantaxantina fue de 152 mg L^-1, 25% más que en un reciente estudio.

Palabras clave: Dietzia maris NIT-D, biomasa, cantaxantina, metodología de superficie de respuesta.

 

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