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Integrated platforms for the clarification and capture of monoclonal antibodies

 

Plataformas integrales para la clarificación y captura de anticuerpos monoclonales

 

S.C. Bernardo, A.M. Azevedo* and M.R. Aires-Barros

 

Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Department of Bioengineering, Instituto Superior Técnico, Technical University of Lisbon, Av Rovisco Pais, 1049-001 Lisbon, Portugal. *Corresponding author. E-mail: a.azevedo@ist.utl.pt Tel. +351218419065, Fax +351218419062.

 

Received May 17, 2013.
Accepted February 14, 2014.

 

Abstract

Antibodies play an important role in both medicine and analytical biotechnology. Recently, the demand for monoclonal antibodies (mAbs) has been increasing exponentially, since their potential for therapeutic use was disclosed and improved techniques to produce them at higher titers have become available. Although upstream processing of mAbs has suffered a tremendous improvement, in the last decades, me downstream processing lias been considered the bottleneck in providing antibodies at reliable costs, being, in fact, the major cost factor with 50 to 80% of total production costs. Therefore, alternative methods for mAbs's purification may be required. Aqueous two-phase systems (ATPS) have been described, for the last decade, as a viable alternative to the current establish platform, since clarification, concentration and purification can be achieved in just one step using a biocompatible environment. Therefore, the use of ATPS for integrative purification of mAbs it will be reviewed.

Keywords: antibodies, capture, purification, aqueous two-phase systems, integrated platform.

 

Resumen

Los anticuerpos tienen un papel importante tanto en la biotecnología médica como en la analítica. Desde que su potencial para uso terapéutico fue descubierto y las técnicas para producirlos en concentraciones más altas fueron mejoradas y se hicieron disponibles, la demanda de anticuerpos monoclonales (mAbs) ha estado incrementado exponencialmente. A pesar de que en las últimas décadas los procesos de fermentación de mAbs han mejorado notablemente, los procesos de recuperación y purificación han sido considerados el cuello de botella para la producción de anticuerpos a precios confiables. De hecho, es esta última etapa la que involucra un mayor costo abarcando del 50 al 80% de los costos totales de producción. Debido a esto, métodos alternativos de producción de anticuerpos son requeridos. Los sistemas de dos fases acuosas (SDFA) han sido descritos en la ultima década, como una alternativa viable a la plataforma actual establecida, dado que la clarificación, concentración y purificación pueden ser llevadas a cabo en un solo paso dentro de un ambiente biocompatible. Es por tanto que el uso de SDFA para una purificación integral será revisado.

Palabras clave: anticuerpos, captura, purificación, sistemas de dos fases acuosas, plataforma integral.

 

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