Biotecnología

 

Un análisis del metabolismo de Aspergillus niger creciendo sobre un sustrato sólido

 

An analysis of the metabolism of Aspergillus niger growing over a solid substrate

 

I. Reyes-Ocampo¹, M. González-Brambila² y F. López-Isunza¹*

 

¹ Departamento de Ingeniería de Procesos e Hidráulica, Universidad Autónoma Metropolitana, Unidad Iztapalapa. Av. San Rafael Atlixco 186 Col. Vicentina, Del. Iztapalapa, México D.F., C.P. 09340, México. * Autor para la correspondencia E-mail: felipe@xanum.uam.mx Tel. 58-04-46-00, Fax 58-04-46-49.

² Departamento de Energía, Universidad Autónoma Metropolitana, Unidad Azcapotzalco. Av. San Pablo 180, Col. Reynosa-Tamaulipas, Del. Azcapotzalco. México D.F., C.P. 02200, México.

 

Recibido 13 de Noviembre de 2012
Aceptado 20 de Enero de 2013

 

Resumen

Se analiza el metabolismo de Aspergillus niger A10 creciendo sobre una placa de agar, utilizando un modelo estructurado que describe a las rutas de la glicólisis (EMP), pentosa-fosfatos (PF), el ciclo de los ácidos tricarboxílicos (CAT), y la producción de biomasa, e incluye la regulación de diferentes enzimas en EMP y CAT en términos de las concentraciones de ácido cítrico y ATP en el micelio. Las predicciones del modelo se apoyan en una serie de experimentos con diferentes concentraciones de glucosa en el medio sólido, así como mezclas binarias de sustratos como fuentes de carbono. Además de las mediciones de biomasa, el CO₂ producido y el O₂ ía (Atkinson, 1969), para analizar la eficiencia del crecimiento a concentraciones de glucosa inicial en la placa de agar que van de 25 a 250 gL^-1.

Palabras clave: metabolismo de Aspergillus niger, sustrato sólido, modelo estructurado, rutas metabólicas, mediciones de biomasa, CO₂ y O₂, caja Petri modificada.

 

Abstract

The metabolism of Aspergillus niger A10 growing on agar plates is analyzed using a structured model that includes the pathways for glicolysis (EMP), pentose-phosphate (PP), the Krebs cycle (TCA), and mycelial biosynthesis; and takes into account enzyme regulation in EMP and TCA either due to low or high levels of citrate and ATP in cytosol. Model predictions are supported by a series of experiments measuring mycelial growth under different glucose concentrations in the agar plates, as well as the use of a two-carbon sources mixtures. Besides measurements of biomass, CO₂ production and O₂ consumption were also continuously measured in the gas-phase of a modified Petri dish. Atkinson's energy charge (1969) concept is used to analyze the growth efficiency under increasing glucose concentration in the agar plates in the range: 25 to 250 gL^-1.

Keywords: Aspergillus niger metabolism, structured model, continuous measurements of CO₂ and O₂, modified Petri dish, biomass growth on solid substrate.

 

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Agradecimientos

Agradecemos al Dr. Hugo Velasco Bedraín (ENCB, IPN) sus importantes comentarios y observaciones a este trabajo, así como al Dr. Ascención Montoya de la Fuente por facilitar la construcción de la caja Petri modificada. El primer autor agradece al Consejo Nacional de Ciencia y Tecnología la beca otorgada (No. 188486) para cursar el Doctorado en Ingeniería Química dentro del Posgrado de Excelencia en Ingeniería Química de la Universidad Autónoma Metropolitana-Iztapalapa.

 

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