Polímeros

 

Quantification of the composition of gum arabic–chitosan coacervates by HPLC

 

Cuantificación de la composición de coacervados de goma arábiga–quitosano por HPLC

 

H. Espinosa–Andrews¹, C. Lobato–Calleros¹, J.M. Loeza–Corte², C.I. Beristain³, M.E. Rodríguez–Huezo² and E.J. Vernon–Carter^2,*

 

¹ DPA and PCyTA, Universidad Autónoma Chapingo, Km 38.5 Carretera México–Texcoco, 56230 Texcoco, México.

² DBT y DlPH. Universidad Autónoma Metropolitana – Iztapalapa. Av. San Rafael Atlixco # 186. Col. Vicentina. 09340 México, D.F., México. * Corresponding author. E–mail: jvc@xanum.uam.mx Tel. +(52) 55 58044648 ext. 233, Fax: +(52) 55 58044900

³ lnstituto de Ciencias Básicas, Universidad Veracruzana, Av. Dr. Rafael Sánchez Altamirano s/n, Col. Industrial–Animas, Apdo. Postal 575, Xalapa, Veracruz., 91000, México.

 

Received 21^st of April 2008
Accepted 17^th of October 2008

 

Abstract

Formation of a complex coacervate between gum Arabic and chitosan produces a spontaneous separation into a coacervate (precipitated) phase coexisting with an equilibrium (soluble) phase. The relative concentrations between the polysaccharides in these two phases, is difficult to quantify. In this work, the equilibrium phase was subjected to acid hydrolysis, and evaluation of the produced monosaccharides was done by HPLC. Composition of the coacervate phase was computed by mass balance. These results were compared with those obtained previously for the same system, where the coacervate phase was analyzed by elementary analysis. Results obtained using both procedures were non–significantly different.

Keywords: gum Arabic, chitosan, complex coacervation, acid hydrolysis, HPLC quantification, equilibrium phase.

 

Resumen

La formación de un coacervado complejo entre la goma arábiga y el quitosano produce una separación espontánea en una fase coacervada (precipitada) que coexiste con una fase en el equilibrio (soluble). No es una tarea sencilla la determinación de las concentraciones relativas entre los polisacáridos en ambas fases. En este trabajo se le efectuó una hidrólisis ácida a la fase en el equilibrio, y se cuantificaron los monosacáridos resultantes por HPLC. La composición de la fase coacervada se determinó por balance de masa. Los resultados obtenidos se compararon con aquellos obtenidos para el mismo sistema utilizando análisis elemental, sin que se hayan encontrado diferencias significativas por ambos métodos.

Palabras clave: goma Arábiga, quitosano, coacervación compleja, hidrólisis ácida, cuantificación por HPLC, fase en el equilibrio.

 

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Acknowledgment

The authors extend their thanks to CONACyT for the partial financing of this project through grant U–52317–Z.

 

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