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Revista mexicana de ingeniería química
versão impressa ISSN 1665-2738
Rev. Mex. Ing. Quím vol.6 no.3 Ciudad de México Dez. 2007
Biotecnología
Larabinose production by hidrolysis of mesquite gum by a crude extract with αLarabinofuranosidase activity from Aspergillus niger
Producción de Larabinosa a partir de la hidrólisis de la goma de mezquite por un extracto crudo con actividad αLarabinofuranosidasa de Aspergillus niger
J. M. LoezaCorte1, J. R. VerdeCalvo1, F. CruzSosa1, E. J. VernonCarter2 and S. HuertaOchoa1*
1 Planta Piloto de Fermentaciones, Departamento de Biotecnología, Universidad Autónoma Metropolitana Iztapalapa. San Rafael Atlixco 186 Col. Vicentina, C.P. 09340, México D.F., México. * Corresponding author: Email: sho@xanum.uam.mx Phone (+52) 5558044999, fax: (+52) 5558044712
2 Laboratorio de Bioprocesos, Departamento de Ingeniería de Procesos e Hidráulica, Universidad Autónoma Metropolitana Iztapalapa. San Rafael Atlixco 186 Col. Vicentina. C.P. 09340, México D.F., México.
Received 25th, April 2007
Accepted 11th, October 2007
Abstract
A crude enzymatic extract from Aspergillus niger 10 with αLarabinofuranosidase activity (EC 3.2.1.55) was obtained and its effect on the hydrolysis of mesquite gum was determined and compared to that of a commercial αLarabinofuranosidase from A. niger. The growth parameters of A. niger 10 obtained were Xmax = 3.03 g L1 and μmax = 0.07 h1. The maximum enzymatic activity obtained was 65.93 U L1. Optimum temperature and activation energy for the crude extract were 50°C and 46.15 KJ mol1 and for the commercial enzyme 40 °C and 52.76 KJ mol1, respectively. The apparent kinetic parameters Km and Vmax for the crude extract were 4.87 g L1 and 0.15 μmol min1 g1, and for the commercial enzyme 76.45 g L1 and 3.85 /mol min1 g1, respectively. Yields of Larabinose recovery for the crude extract and the commercial enzyme were 17.04 % and 2.78 %, respectively, based on the reported average content of Larabinose in mesquite gum.
Keywords: αLarabinofuranosidase, Aspergillus niger, mesquite gum, Larabinose, enzymatic hydrolysis.
Resumen
Se obtuvo un extracto enzimático crudo a partir de Aspergillus niger 10 con actividad αLarabinofuranosidasa (EC 3.2.1.55) y fue comparado con una αLarabinofuranosidasa comercial sobre la hidrólisis enzimática de la goma de mezquite. Los parámetros de crecimiento obtenidos para A. niger 10 fueron: Xmax = 3.03 g L1 y μmax = 0.07 h1. La máxima actividad enzimática obtenida fue 65.93 U L1. La temperatura óptima y energía de activación para el extracto crudo fueron de 50°C y 46.15 KJ mol1, mientras que para la enzima comercial fueron de 40°C y 52.76 KJ mol1. Los parámetros cinéticos de la hidrólisis Kmapp y Vmaxapp con el extracto crudo fueron de 4.87 g L y 0.15 /mol min1 g1, y para la enzima comercial fueron de 76.45 g L1 y 3.85 μmol min1 g1, respectivamente. Los rendimientos de Larabinosa con el extracto crudo y la enzima comercial fueron de 17.04 % y 2.78 %, respectivamente, basados en lo reportado para el contenido promedio de Larabinosa en la goma de mezquite.
Palabras clave: αLarabinofuranosidasa, Aspergillus niger, goma de mezquite, Larabinosa, hidrólisis enzimática.
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