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Revista mexicana de ingeniería química

versión impresa ISSN 1665-2738

Rev. Mex. Ing. Quím vol.6 no.3 México dic. 2007

 

Biotecnología

 

L–arabinose production by hidrolysis of mesquite gum by a crude extract with α–L–arabinofuranosidase activity from Aspergillus niger

 

Producción de L–arabinosa a partir de la hidrólisis de la goma de mezquite por un extracto crudo con actividad α–L–arabinofuranosidasa de Aspergillus niger

 

J. M. Loeza–Corte1, J. R. Verde–Calvo1, F. Cruz–Sosa1, E. J. Vernon–Carter2 and S. Huerta–Ochoa1*

 

1 Planta Piloto de Fermentaciones, Departamento de Biotecnología, Universidad Autónoma Metropolitana Iztapalapa. San Rafael Atlixco 186 Col. Vicentina, C.P. 09340, México D.F., México. * Corresponding author: E–mail: sho@xanum.uam.mx Phone (+52) 5558044999, fax: (+52) 5558044712

2 Laboratorio de Bioprocesos, Departamento de Ingeniería de Procesos e Hidráulica, Universidad Autónoma Metropolitana Iztapalapa. San Rafael Atlixco 186 Col. Vicentina. C.P. 09340, México D.F., México.

 

Received 25th, April 2007
Accepted 11th, October 2007

 

Abstract

A crude enzymatic extract from Aspergillus niger 10 with α–L–arabinofuranosidase activity (EC 3.2.1.55) was obtained and its effect on the hydrolysis of mesquite gum was determined and compared to that of a commercial α–L–arabinofuranosidase from A. niger. The growth parameters of A. niger 10 obtained were Xmax = 3.03 g L–1 and μmax = 0.07 h–1. The maximum enzymatic activity obtained was 65.93 U L–1. Optimum temperature and activation energy for the crude extract were 50°C and 46.15 KJ mol–1 and for the commercial enzyme 40 °C and 52.76 KJ mol–1, respectively. The apparent kinetic parameters Km and Vmax for the crude extract were 4.87 g L–1 and 0.15 μmol min–1 g–1, and for the commercial enzyme 76.45 g L–1 and 3.85 /mol min–1 g–1, respectively. Yields of L–arabinose recovery for the crude extract and the commercial enzyme were 17.04 % and 2.78 %, respectively, based on the reported average content of L–arabinose in mesquite gum.

Keywords: α–L–arabinofuranosidase, Aspergillus niger, mesquite gum, L–arabinose, enzymatic hydrolysis.

 

Resumen

Se obtuvo un extracto enzimático crudo a partir de Aspergillus niger 10 con actividad α–L–arabinofuranosidasa (EC 3.2.1.55) y fue comparado con una α–L–arabinofuranosidasa comercial sobre la hidrólisis enzimática de la goma de mezquite. Los parámetros de crecimiento obtenidos para A. niger 10 fueron: Xmax = 3.03 g L–1 y μmax = 0.07 h–1. La máxima actividad enzimática obtenida fue 65.93 U L–1. La temperatura óptima y energía de activación para el extracto crudo fueron de 50°C y 46.15 KJ mol–1, mientras que para la enzima comercial fueron de 40°C y 52.76 KJ mol–1. Los parámetros cinéticos de la hidrólisis Km–app y Vmax–app con el extracto crudo fueron de 4.87 g L y 0.15 /mol min–1 g–1, y para la enzima comercial fueron de 76.45 g L–1 y 3.85 μmol min–1 g–1, respectivamente. Los rendimientos de L–arabinosa con el extracto crudo y la enzima comercial fueron de 17.04 % y 2.78 %, respectivamente, basados en lo reportado para el contenido promedio de L–arabinosa en la goma de mezquite.

Palabras clave: α–L–arabinofuranosidasa, Aspergillus niger, goma de mezquite, L–arabinosa, hidrólisis enzimática.

 

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