SciELO - Scientific Electronic Library Online

 
vol.71 número1Adiciones a los gasterópodos continentales del Cretácico tardío y el Paleoceno del NE de MéxicoGeocronología de depósitos minerales mexicanos. VIII: el skarn polimetálico de Zacatepec, Oaxaca índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

  • Não possue artigos similaresSimilares em SciELO

Compartilhar


Boletín de la Sociedad Geológica Mexicana

versão impressa ISSN 1405-3322

Bol. Soc. Geol. Mex vol.71 no.1 México Abr. 2019

https://doi.org/10.18268/bsgm2019v71n1a10 

Notas cortas

Abies cuitlahuacii sp. nov. a mummified Late Quaternary fossil wood from Chalco, Mexico

Abies cuitlahuacii sp. nov., madera fossil momificada del Cuaternario tardío de Chalco, México

Sergio, R.S. Cevallos-Ferriz1 

César Ríos-Santos2 

Socorro Lozano-García1 

1Instituto de Geología, Universidad Nacional Autónoma de México, Ciudad Universitaria, Circuito de la Investigación Científica, Coyoacán 04510 CDMX, México. oasis08_1@hotmail.com

2Posgrado en Ciencias Biológicas, Instituto de Geología, Universidad Nacional Autónoma de México, Ciudad Universitaria, Circuito de la Investigación Científica, Coyoacán 04510 CDMX, México.


Abstract

A fragment of mummified wood collected from a Quaternary sedimentary sequence of Lake Chalco, Central Mexico, in the depocenter of the Basin (19°15ʹ26ʺN, 98°58ʹ32ʺW), has anatomical characteristics of Abies. Identification is based on the presence of a warty layer in the walls of the tracheids, prismatic crystals in ray cells, taxodioid pits in the cross-fields, and lack of ray tracheids and normal intercellular canals, among other well-preserved characteristics. This specimen represents the first macrofossil evidence of the genus in this region and suggests that by the Late Quaternary it was already established in the surroundings of Lake Chalco, and most probably in what is now central Mexico. Unfortunately, as with morphological and genetic characteristics, the anatomical attributes of the wood of the genus have not been useful for the delimitation of species. Most wood characteristics have been considered of little diagnostic value. However, comparison of available anatomical descriptions of fossil and living species highlight differences among them, even with Abies religiosa wood, which is the closest species to Lake Chalco. Other characteristics or plant organs are needed to assemble a whole plant that can be used to establish phylogenetic relationships and clarify the evolutionary history of the genus. Meanwhile this wood is assigned to a new fossil taxon, Abies cuitlahuacii Cevallos-Ferriz, Ríos-Santos & Lozano-García.

Keywords: wood; Lake Chalco; anatomical characteristics; Late Quaternary; Abies cuitlahuacii

Resumen

Un fragmento de madera momificada recolectada de una sección de una secuencia sedimentaria del Lago de Chalco, en el centro de México, en particular en el depocentro de la Cuenca (19°15'26" N, 98°58'32" W), tiene características anatómicas de Abies. Esto se basa en la presencia de una capa verrugosa en las paredes de las traqueidas, cristales prismáticos en las células radiales, punteaduras taxodioides en los campos de cruce y ausencia de traqueidas radiales y canales normales, entre otras características bien preservadas. Este especímenrepresenta la primera evidencia con macrofósil del género en esta región y sugiere que para el Cuaternario tardío ya estaba establecido el género en los alrededores del Lago de Chalco y por lo tanto, en lo que hoy es el centro del país. Desafortunadamente, como ocurre con los caracteres morfológicos y genéticos, los atributos anatómicos de la madera del género tampoco han sido útiles para la delimitación de especies, ya que la mayoría de ellos son considerados poco diagnósticos. Sin embargo, en este estudio se hicieron comparaciones con maderas de especies fósiles y vivas disponibles, y en todas se encontraron diferencias anatómicas para con el ejemplar fósil, incluso con la madera de Abies religiosa que es la especie que tiene distribución más cercana al Lago de Chalco. En espera de que sean propuestos otros caracteres o estudios integrales de ellos que sirvan como auxiliaries para la delimitación de los miembros que constituyen al género, o de hallar otros órganos fósiles en la área de estudio que complementen la identificación aquí presentada, esta madera es asignada a una especie nueva, Abies cuitlahuaciiCevallos-Ferriz, Ríos-Santos & Lozano-García.

Palabras clave: Madera momificada; Lago de Chalco; características anatómicas; Cuaternario tardío; Abies cuitlahuacii

1. Introduction

The basin of Mexico is located in the geological province of the Trans-Mexican Volcanic Belt (TMVB), a continental arc active since the middle Miocene (Gómez-Tuena et al., 2005). Recent studies documented that the basin was closed ca. 1.2 Ma ago (Arce et al., 2013) leading to the development of a lacustrine system. This lake system reached an area of 1689 km2by the 14th century (Ruiz-Angulo and López-Espinosa, 2015). Part of this system corresponds to the modern Lake Chalco (19°15ʹ26ʺN, 98°58ʹ32ʺW, 2230 meters above sea level (masl)). The lake has an area of 1100 km2, of which approximately 240 km2 correspond to the lacustrine plain. The environmental history of the area is archived and has been disentangled from the late Quaternary sedimentary rocks. of Lake Chalco (Table 1; Lozano-García et al., 1993;Urrutia-Fucugauchi et al., 1995; Caballero and Ortega, 1998; Ortega-Guerrero et al., 2000, 2017; Lozano-García et al., 2015). The previous studies demonstrated that temperate forests make up the dominant vegetation types of Central Mexico's mountains today. These forests consist of Pinus L. 1753, Pinus-Quercus L. 1753, Abies, and/o Juniperus L. 1753, and developed within elevations that range from 1500 to 4000 m. Abies forests are restricted to humid areas such as glens between 2600 and 3600 m and temperatures between 7 and 17 °C; however, they are sometimes intermixed with pine-oak forests, although they may form pure stands. These forests are located in the southern portion of the Basin of Mexico.

Table 1 Fir species distributed in Mexico (Farjon 2010). 

Species Varieties Distribution
Abies concolor (Gordon) Lindl. ex Hildebr. Mexico: Baja California, Chihuahua y Sonora; USA: from Oregon to Idaho through the Rocky Mountains and the California and Arizona Hills.
A. durangensis Martínez A. durangensis Martínez var. durangensis Chihuahua, Durango, Northern zones of Jalisco and Sinaloa.
A. durangensis var. coahuilensis (I.M. Johnst.) Martínez Coahuila
A. guatemalensis Rehd. A. guatemalensis Rehd. var. guatemalensis Mexico: from Chiapas to Nayarit and Tamaulipas; El Salvador; Western Guatemala; and Honduras.
A. guatemalensis Rehd. var. jaliscana Martínez Jalisco
A. hickelii Flous & Gaussen A. hickelii Flous Gaussen var. hickelii Chiapas, Guerrero and Oaxaca.
A. hickelii Flous & Gaussen var. oaxacana (Martínez) Farjon & Silva Guerrero and Oaxaca
A. hidalgensis Debreczy, Rácz & Guízar Hidalgo
A. religiosa (Kunth) Schltdl. & Cham. High zones of Central and Southern Mexico; Western Guatemala.
A. vejarii Martínez A. vejarii Martínez var. vejarii, Oriental Sierra Madre in Coahuila, Nuevo León and Tamaulipas.
A. vejarii Martínez var. macrocarpa Martínez Coahuila (Mesa de las Tablas) and Nuevo León (Cerro Potosí).
A. vejarii Martínez var. mexicana (Martínez) T.S. Liu Southeastern Coahuila and Nuevo León (Sierra Santa Catarina).

Several studies (Lozano-García and Ortega-Guerrero, 1994; Lozano-García et al., 1993; 1998; Lozano-García, 1996; Torres-Rodríguez et al., 2015) have documented changes in the composition of communities in response to late Pleistocene climatic changes. They are based on the presence of open Pinus forests during cold periods, such as the last glacial maximum, where temperatures were reduced by 6 to 8 °C relative to the pre-glacial period (Vázquez-Selem and Heine, 2011; Correa-Metrio et al., 2013). As the climate warmed at the beginning of the Holocene, pollen records show an increase in the associatedAbiesMill. 1754 forest communities due to the increase in humidity in the area (Lozano-García et al., 1993; Lozano-García and Ortega-Guerrero, 1994, 1998; Lozano-García, 1996; Torres-Rodríguez et al., 2015).

The climate corresponding to the great majority of the modern forests of these species in Mexico, according to the classification ofKöppen (1948), is Cw (dry winter). The geological substrates in which these forests developed are varied. The most predominant ones are those of volcanic origin, mainly andesites and basalts (Rzedowski, 2006).

Xiang et al. (2007) proposed that Abies originated during the Cretaceous. However, more recent molecular and biogeographic studies have estimated its origin in the Eocene, possibly in western North America, or in North America and East Asia when these were connected via the Bering Land Bridge (Xiang et al., 2015).

There is no macrofossil evidence ofAbiesin the fossil record in Mexico. However, there is a palynological record of the genus in the Pliocene from Paraje Solo, Veracruz (Graham, 1976, 1999). The palynological record of the area, including older Cenozoic strata in the same geographic region, strongly suggests that Abies and probably other conifer pollen grains were dispersed from the north (Graham, 1999). These data, coupled with the existing diversity of the genus in this part of the North American continent, suggest a rapid diversification shortly after their establishment (Aguirre-Planter et al., 2012). A similar history was proposed by Huerta-Vergara (2014) for Pinus in Mexico suggesting its relatively recent establishment in the region.

Although little is known about the fossil record ofAbiesin Mexico, the Pliocene has been proposed as the time of its arrival to the area. This is based on palynological records and the discussion of plant communities whereAbieshas been found, especially in high latitude North America (Graham, 1999). At that time, it formed part of lowland forests of mixed conifers and broadleaf deciduous trees adjacent to coastal mangroves. This contrasts with the extant distribution of the plants that formed these types of vegetation. While mangroves grow at sea level next to tropical floras, more temperate elements establish their distribution high in the mountains (Graham, 1976). In contrast, molecular studies have proposed that the origin of Mesoamerican firs (a group with species from southwestern North America of sections Oiamel and Grandis; Farjon and Rushforth, 1989) dates from late Paleocene and early Miocene, after a few southward expansions that these groups probably followed through the mountain chains of western North America (Aguirre-Planter et al., 2012).

2. Methodology

The drilling of the sedimentary sequence of Chalco was in the depocenter of the basin (19°15ʹ26ʺN, 98°58ʹ32ʺW). Three parallel cores were drilled with the aim of retrieving the entire sequence. The three wells are well A (10 to 420 m), well B (6 to 310 m), and well C (10 to 300 m and 409 to 520 m). The upper 300 m of lacustrine sediments were recovered with a percussion HQ system with diamond drill in section lengths of 1.5 m (Lozano-García et al., 2017). The documentation and description of the sequences were performed at the University of Minnesota Lacustrine Core Facility (LacCore) and included the measurement of magnetic susceptibility and gamma density with a Geotek Multi-Sensor Core Logger. Once open, the sections were described and photographed with a DMT CoreScan digital linescan camera. During the description process of sequence B corresponding to a depth of 198.5 m a piece of wood embedded in volcanic ash was recovered (Figure 1).

Figure 1 Location map. A. Basin of Mexico. B. Lake ChalcoBasin of Mexico (19°15ʹN, 98°58ʹW, 2230 masl). X indicates the drilling site. C. Simplified and combined stratigraphic column of wells A and B (Bücker et al., 2017; Lozano-Garcíaet al., 2017). D. Specimen of the wood recovered in section 1B-132 of well B. 

Eight slides were obtained with the conventional thin-section technique oriented on the three cutting planes of the wood. The observation of the anatomical characteristics was done with a Carl Zeiss Primo Star optical microscope and the photographs of the characteristics were obtained with a Canon PowerShot A640 camera. Anatomical descriptions follow Baas et al. (2004), García-Esteban et al. (2002), and Barefoot and Hankins (1982). As for the quantitative traits, 30 measurements for each attribute were obtained. However, ray height is based on 100 measurements. It should be emphasized that both the observations and the measurements of attributes were made from the early wood. Values are expressed as average (minimum-maximum), or in sporadic cases, with the intention of better expressing their distribution, as minimum-average-maximum (occasional maximum). Identification of the sample is based on the already mentioned literature, but additional works were consulted for important taxonomical decisions (e.g., Greguss, 1955; García-Esteban et al., 2004, 2009; Samerikova and Semerikov, 2014).

3. Results

Systematics

Family: Pinaceae Spreng. ex F. Rudolphi, 1830.

Genus:AbiesMill., 1754.

Species:Abies cuitlahuacii, Cevallos-Ferriz, Ríos-Santos & Lozano-García.

Etymology: The specific epithet honors Cuitláhuac, the penultimate “Huey Tlatoani”; a Nahuatl expression that refers to governors of the triple alliance (Tenochtitlán-Texcoco-Tlacopan) of the regions where the lake system occurred.

Repository: Paleontological Collection of the Instituto de Geología, Universidad Nacional Autonoma de México, Mexico.

Holotype: IGL-CHA16-B: 1-8.

Number of specimens examined: 1.

Thin-sections: 8.

Location: Lake Chalco.

Coordinates: 19°15ʹ26ʺN, 98°58ʹ32ʺW.

Hole: CHA-SEQ-2016-B.

Depth: 198.5 m.

Age: ca. 200000 yrs (Pleistocene; estimation based on previous studies in lake Chalco (Torres-Rodríguezet al., 2015; Ortega-Guerrero et al., 2017).

Diagnosis: Pycnoxilic wood with distinguishable growth ring boundaries; tracheid walls with warty layer, trabecula and uniseriate abietinean bordered pits on radial and tangential walls; bordered pits occasionally with torus extension; axial parenchyma with brown contents, nodular transverse end walls, and tangentially zonate arrangement; uniseriate and partially biseriaterays, 19.5-25(37) cells high, ray parenchyma cells with pitted horizontal walls, nodular end walls, brown contents and prismatic crystals in their lumens, occasionally irregularly shaped parenchyma cells; taxodioid cross-fields pits, with 1-2 pits per cross-field and average diameter of 4.6 × 3 µm; resin canals and ray tracheids absent.

Figure 2 Photomicrographs ofAbies cuitlahuacii. TR. A. Distinguishable growth ring boundaries generally with abrupt transition (black arrows). Axial parenchyma tangentially zoned (white arrows). Scale bar = 1 cm. B. Tracheids of quadrangular outlines. Axial parenchyma (white arrows) and ray parenchyma cells (black arrows) with brown contents. Scale bar = 65 μm. C. Overview of the radial section. Rays composed exclusively of radial parenchymal cells. Scale bar = 200 μm. D. Radial walls of tracheids with warty layer (arrows). Scale bar = 20 μm. E. Tracheids with abietinean radial pitting. Scale bar = 100 μm. F. Bordered pits with their torus extensions (arrow). Scale bar = 15 μm. G. Ray parenchyma cells with horizontal pitted horizontal walls (arrows). Scale bar = 60 μm. H. Radial parenchymal cells with nodular axial walls (arrow). Scale bar = 20 μm. I. Ray parenchyma cell with abnormal shape in the margin of a ray (arrow). Scale bar = 40 μm. 

Description: Mummified wood fragment 1.5 cm in diameter by 2 cm in length, with distinguishable growth ring boundaries with normal abrupt transition (Figure 2A). The late wood consists of approximately 6 to 27 rows of tracheids. The tracheids have quadrangular outlines in cross section (Figure 2B) and on average there are 2090 tracheids/mm2. The radial and tangential diameters of the early Wood tracheids is 20 (16-29) μm and 21.6 (17-30) μm, respectively. In the lumen trabeculae (Figure 3H) can be observed sporadically. The walls of tracheids are 2 μmthick on average and have a warty layer (Figure 2D). The radial walls of the tracheids have abietinoid bordered pits arranged in a single series (Figure 2E), with circular contours and openings, diameter of 13.8 (12.5-15) μm and with extended torus (Figure 2F); the tangential walls have the same type of bordered pits, but unlike the bordered pits on the radial walls, they are more sporadic and slightly smaller in diameter, 10.7 (8-13) μm (Figure 3G). Axial parenchyma cells have a tangentially zoned arrangement, particularly in the transition from the early to the late wood (Figure 2A); parenchyma cells have obscure contents (Figure 2B) and nodular transverse walls (Figure 3I). The rays are uniseriate (Figure 3E) and sometimes partially biseriate (Figure 3F); their height varies from 1-9.5-25 (37) cells and their frequency is 9.2 rays/mm; they are composed of rectangular parenchyma cells (Figure 2C and G) with occasional irregular formed cells in the ray margins (Figure 2H). Ray parenchyma cells have pitted horizontal walls (Figure 2G), nodular end walls (Figure 2H), brown contents (which could be some type of resin, Figure 2B), and furthermore rarely indentures (Figure 3A) and crystals (Figure 3B). One or occasionally 2 taxodioid pits present per cross-field, with an average diameter of 4.6 × 3 μm (Figure 3C and D). The wood lacks resin canals and radial tracheids.

Figure 3 Photomicrographs ofAbies cuitlahuacii. RA. A. Ray parenchyma cell with radial indentures (arrow). Scale bar = 40 μm. B. Ray parenchyma cell with a quadrangular crystal (arrow). Scale bar = 50 μm. C. Overview of the cross-fields. Scale bar = 80 μm. D. Taxodioid pits in cross-fields. Scale bar = 35 μm. TA. E. Uniseriate rays. Scale bar = 200 μm. F. Partially biserial rays (arrows). Scale bar = 100 μm. G. Bordered pits on tangential walls of tracheids (arrows). Scale bar = 40 μm. H. Tracheids with trabeculae (arrow). Scale bar = 40 μm. I. Axial parenchyma cells with nodular transverse walls (arrow). Scale bar = 20 μm. 

4. Discussion and conclusion

4.1 Comparison with extant plants

The anatomical structure of the wood of Pinaceae is unique for having attributes that are not normally present in other conifer families. These unique attributes have been recognized as anatomical apomorphic characteristics among living conifers. These include: normal resin canals, pinoid, window or piceoid cross-field pits, and radial tracheids, among others (Greguss, 1955). However, of all the genera that make up Pinaceae,PseudolarixGordon, 1858,KeteleeriaCarrière, 1866, andAbies(three of the six circumscribed genera in the subfamily AbietoideaePilg.;Farjon, 2010) have simpler structure (Greguss, 1955) and more similar anatomy to the wood described in this study. Moreover,Pseudolarixlacks the warty layer on the walls of its tracheids (García-Esteban et al., 2009) and has a larger number of pits (3-6) per cross-field (Greguss, 1955). Also,Keteleeriais distinguished by having axial resin canals (García-Esteban et al., 2009), which prevents the association of Chalco wood with this genus. Therefore, the wood described in this contribution is included inAbiesbecause both share the warty layer in the walls of the tracheids, prismatic crystals in ray parenchyma cells, and taxodioid cross-field pitting (Greguss, 1955; García-Esteban et al., 2009). However, in previous anatomical studies (e.g. García-Esteban et al., 2002, 2004),Abieswoods have been described with cross-field pitting with piceoid pits. In addition to the characteristics mentioned above, there are others that were observed in the fossil wood that reinforce its identification despite not being completely diagnostic forAbies. Among them are the exclusively uniseriate abietinoid bordered pits on the radial and tangential walls of the tracheids, with bordered pits with torus extensions; tangentially zoned axial parenchyma with nodular transverse walls; uniseriate to partially biseriate rays; ray parenchyma with pitted horizontal and nodular end walls; and finally a lack of radial tracheids and normal resin canals (Barefoot and Hankins, 1982; García-Esteban et al., 2002, 2004, 2009; Baas et al., 2004; Samerikova and Semerikov, 2014).

According toGarcía-Esteban et al. (2009), there are no qualitative anatomical differences that can be used to separate species of this genus. In fact, this has also been observed with morphological and genetic characters (Martínez-Méndez et al., 2016). Thus, delimitation of species of this genus has proved so difficult that there is no consensus on how many species actually exist (Strandby et al., 2009; Martínez-Méndez et al., 2016). Particularly, the Mesoamerican firs probably are still in a gray zone of speciation (sensude Queiroz, 1998, 2005), where due to the recent time of separation of the populations, the reciprocal monophyly is incomplete and populations have not yet formed phenotypically and ecologically distinguishable or diagnosable groups (Martínez-Méndez et al., 2016). This situation supports that the assignment of this fossil wood to a living species is complicated. Although this fossil wood is similar to the wood ofAbies religiosa,(Kunth) Schltdl. & Cham., 1830, a species whose geographical distribution is the closest to the locality where the fossil wood was collected (less than 20 km from Mexico City), some characteristics in the extant plants were not observed in the wood from Chalco. These are, helical thickenings in the tracheids, biseriate bordered pits, bars of Sanio,piceoid pits in the cross-fields, and lower rays with a maximum of 6 cells high (García-Esteban et al., 2002, 2004, 2009). There are no studies describing the wood anatomy of most of the Mesoamerican fir species. Exceptions are Abies guatemalensis Rehd., 1939 and Abies concolor (Gordon) Lind. Ex Hildebr., 1861. The first one can be distinguished from this fossil wood by having xylem with biseriate bordered pits, bars of Sanio, 1-4 piceoid pits per cross-field, and the absence of torus extensions; while the second one generally has lower rays (1-4 (12) cells), marginal axial parenchyma, and piceoid cross-field pits. Despite these differencesA. concolorhas wood with greater similarity to the fossil wood, probably because it has been considered a species more divergent within the Mesoamerican firs (Aguirre-Planter et al., 2012; Martínez-Méndez et al., 2016).

4.2 Comparison with fossil woods

The record of fossil woods related to Abies is not extensive and is restricted toAbietoxylon Houelbert, 1910 andAbiesMill 1754. In North America, Abietoxylon koreanoidesRoy & Hill, 1972 andAbietoxylon traumatiductusRoy & Hill, 1972 were described from the Beaufort Formation (Miocene-Early Pliocene) in northwest Banks Island in the Canadian Arctic Archipelago.AbiesfirmoidesWateri, 1956 was described from deposits of the lower Oligocene of Japan.Abies chavchavadzeaeBlokhina & Bondarenko, 2008 was described along withAbiesaff.sachalinensesfrom the lignite field of Pavlovka in the Primorskii Region, Pliocene of Russia.Abietoxylon shakhtnaenseBlokhina, 2010has also been reported from Russia, but in deposits of the upper Oligocene-lower Miocene in the east of Sakhalin. Finally, the wood ofAbietoxylon faudenseHoulbert, 1910 was described from Faluns Formation, Miocene of Touraine, France.

Abietoxylon koreanoidesis distinguished from the Chalco wood based on lower rays (2-8 (10) cells high), more piceoid to taxodioid pits per cross-field (2-4), and lack of bordered pits on tracheids tangential walls (Roy and Hill, 1972).Abietoxylon traumatiductusdiffers from the Mexican wood because it has traumatic resin canals, relatively lower rays (2-12 (15) cells), occasional helical thickenings in tracheids of early wood, axial parenchyma with smooth transverse walls, and a greater number of taxodioid pits per cross-field (2-4; Roy and Hill, 1972).Abies firmoides, in contrast toAbies cuitlahuacii, has biseriate bordered pits with bars of Sanio (or crassulae), and axial parenchyma with smooth and nodular transverse end walls, as well as a greater number of pits per cross-field (2-5;Watari, 1956).Abies chavchavadzeaeunlike the Chalco wood has uniseriate and biseriate bordered pits, bars of Sanio associated with biseriatebordered pits, higher rays (1-51 cells), and greater number of taxodioid pits per cross field (1-3 (4);Blokhina and Bondarenko, 2008).Abietoxylon shakhtnaenseis distinguished from the new wood because it has higher rays 1-30 (50-60), greater number of taxodioid pits per cross-field (1-3 (4), occasionally biseriate bordered pits, and traumatic resin canals (Blokhina, 2010).

Abietoxylon faudenseHoulbert is the type species of the genus, and Philippe and Bamford (2009) reviewed the original material of this fossil taxon. Since there was no holotype assigned in the original publication, a lectotype was proposed under the name ofAbietoxylon falunense. Since the lectotype, like the rest of the syntypes, lacked good preservation, it was not possible to identify the anatomical diagnostic characteristics for its correct identification. In fact, it was not possible to know what the diagnostic characteristics were for the genus because Houelbert (1910) never indicated them. Philippe and Bamford (2009) added that the quantitative characteristics of this type of wood are very similar to the characteristics ofTaxoxylon, another type of wood that Huelbert (1910) described. They continued arguing that these genera, along withCupressoxylonandJuniperoxylon, described in the same publication, probably belong to a single taxon and suggested not to use this fossil genus. The review of this taxonomic problem is beyond the scope of this paper; however, a taxonomic revision of the genus is needed. The anatomical characteristics in common between the fossil woods and that differ with the current woods are higher rays, bordered pits on the radial walls of the tracheids, traumatic canals, and axial parenchyma cells with a combination of smooth and nodular transverse end walls.

Because of the age of the fossil wood of Mexico (Pleistocene), the difficulty in identifying extant species ofAbies, and the anatomical differences founded both in extant and fossil woods (although we do not rule out the possibility that some characteristics that we consider in anatomical comparisons are more related to the phenotypic plasticity of wood; for example, the number of pits in the cross-field and biseriate bordered pits), the new wood is recognized as a member of the genus, and as a new species,Abies cuitlahuicii. However, the need to find other macrofossils of this genus in the same locality to assemble them under the whole plant reconstruction hypothesis is highlighted. Moreover, new integrative studies about different characteristics ofAbieswill be necessary to do a detailed comparison that backs-up our taxonomic decision and also to know about its more precise phylogenetic relationship.

4.3 The genus in Mexico

The new fossil,Abies cuitlahuacii, represents the first macrofossil of this genus in Mexico, and thus the first direct evidence of the species growing close to the area of deposition. The associated plants in the studied nuclei represent both mixed conifers and broadleaf deciduous forests, as expected, but compared with previous fossil records,Abies cuitlahuaciiwas growing at over 2000 masl.

Abiesis the genus with the second highest number of species (47 spp.) of Pinaceae (Farjon, 2010). Most of the species of this family are mesophyll elements of the boreal and temperate forests of the Northern Hemisphere, which together with species ofPiceaA. Dietr.,Tsuga(Endl.) Carrière,PseudotsugaCarrière, andPinusL. (subgenus Strobus) conform conifer forests in high mountain belt areas where humidity is high and constant (Semerikova and Semerikov, 2014).Abies cuitlahuaciiprobably represents a plant growing at a moment in which the genus had its greatest distributional area in Central Mexico. Flores-Mata et al. (1971) estimated that theAbiesforests covered during the Pleistocene approximately 0.16% of México, but this area most likely has been reduced, primarily by anthropogenic activity. This type of vegetation is represented in small patches, often restricted to a hill, a slope, or a glen, and at altitudes usually between 2400 and 3000 m (Rzedowski, 2006).

It is proposed here thatAbies cuitlahuaciiformed part of the stem group that gave rise to the extant species growing today near the area (e.g.,A. religiosa,A. guatemalensis,A. hickelii). Further studies will clarify this proposal and establish the relationship of the fossil plant with the seven fir species that grow naturally in Mexico, more than half of them with two or three varieties (Farjon, 2010; see Table 1). The appearance ofAbiesin the Pleistocene in Mexico seems similar to the proposed relatively recent history of extantPinusin the area. Their history highlights the importance of geological evolution in the advancement of biodiversity in low latitude North America (Huerta-Vergara, 2014).Abies cuitlahuaciirepresents another example of the lineages that diversified as the mountain ranges develop in central Mexico, highlighting the close and important relationship of the geological and biological processes (Cevallos-Ferriz et al., 2012). The inferred cycles of isolation and contact thatAbiesspecies experienced in this geographical region were influenced by their ecological monitoring towards temperate conditions related to phases of maximum glaciers and interglacial periods, and gradual uplift of the TMVB from the upper Miocene (Gómez-Tuena et al., 2005; Bush et al., 2009; Ramírez-Barahonaand Guiarte-Fruns, 2013).

Acknowledgments

This project was funded by the following projects: UNAM DGAPA-PAPITT IV100215 and IN210416, International Scientific Drilling Program (ICDP Project 05-2014), US National Science Foundation (NSF-EAR1551311), and CONACYT-Mexico 221129. We thank Susana Sosa Nájera and Cecilia Caballero for assistance during the fieldwork and laboratory tasks.

References

Aguirre-Planter, E., Jaramillo-Correa, J.P., Gómez-Acevedo, S., Khasa, D.P., Bousquet, J., Eguiarte, L.E., 2012, Phylogeny, diversification rates and species boundaries of Mesoamerican firs (Abies, Pinaceae) in a genus-wide context: Molecular Phylogenetics and Evolution, 62(1), 263-274. [ Links ]

Arce, J., Layer, P.W., Lassiter, J.C., Benowitz, J.A., Macías, J.L., Ramírez-Espinosa, J., 2013, 40Ar/39Ar dating, geochemistry, and isotopic analyses of the quaternary Chichinautzin volcanic field, south of Mexico City: implications for timing, eruption rate, and distribution of volcanism: Bulletin of Volcanology, 75, 774. [ Links ]

Barefoot, A.C., Hankins, F.W., 1982. Identification of modern and tertiary woods: Oxford, U.K., Oxford University Press, 189 p. [ Links ]

Baas, P., Blokhina, N., Fujii, T., Gasson, P., Heinz, I., Ilic, J., Xiaomei, J., Miller, R., Newsom, L.A., Noshiro, S., Richter, H.G., Suzuki, M., Terrazas, T., Wheeler, E., Wiedenhoeft, A., 2004, IAWA List of microscopic features for softwood identification: IAWA Journal, 25(1), 1-70. [ Links ]

Blokhina, N. I., 2010, Fossil wood ofAbietoxylon shakhtnaensesp. nov. (Pinaceae) from the Upper Oligocene-Lower Miocene deposits of southeastern Sakhalin: Paleontological Journal, 44(3), 348-355. [ Links ]

Blokhina, N., Bondarenko, O.V., 2008, New species of pinaceous fossil wood from the Pliocene of southern Primorye (Russian Far East): Paleontological Journal, 42(2), 203-210. [ Links ]

Bücker, M., Lozano-García, S., Ortega-Guerrero, B., Caballero, M., Pérez, L., Caballero, L., Pita de la Paz, C., Sánchez-Galindo, A., Villegas, F. J., Flores-Orozco, A, et. al. 2017, Geoelectrical and electromagnetic methodsapplied to paleolimnological studies: Two examples from desiccated lakes in the Basin of Mexico: Boletín de la Sociedad Geológica de México, 69(2), 279-298. [ Links ]

Bush, M.B., Correa-Metrio, A.Y., Hodell, D.A., Brenner, M., Anselmetti, F.S., Ariztegui, D., Mueller, A.D., Curtis, J.H., Grzesik, D.A., Burton, C., Gilli, A., 2009, Re-evaluation of climate change in lowland Central America during the last glacial maximum using new sediment cores from Lake Petén Itzá, Guatemala, in Vimeaux F., Sylvestre, F., Khodri, M. (eds.), Past Climate Variability in South America and Surrounding Regions: From the Last Glacial Maximum to the Holocene: Dordrecht, The Netherlands, Springer, 113-128. [ Links ]

Caballero, M. , Ortega-Guerrero, B. , 1998, Lake levels since about 40000 years ago at Lake Chalco, near Mexico City: Quaternary Research, 50(1), 69-79. [ Links ]

Cevallos-Ferriz, S.R.S., González-Torres, E.A., Calvillo-Canadell, L., 2012, Perspectiva paleobotánica y geológica de la biodiversidad en México: Acta Botánica Mexicana, 100, 317-350. [ Links ]

Correa-Metrio, A., Bush, M.B. , Lozano-García, S. , Sosa-Nájera, S., 2013, Millennial-scale temperature change velocity in the continental northern Neotropics: PloS ONE, 8(12), e81958. [ Links ]

de Queiroz, K., 1998, The general lineage concept of species, species criteria, and the process of speciation: A conceptual unification and terminological recommendations, in Howard, D.J., Berlocher, S.H. (eds), Endless forms: Species and Speciation: Oxford, U.K., Oxford University Press, 57-75. [ Links ]

de Queiroz, K. 2005, A unified concept of species and its consequences for the future of taxonomy: Proceedings of the California Academy of Sciences, 56(1) ,196-215. [ Links ]

Farjon, A., 2010, A handbook of the world’s conifers: Leiden, The Netherlands, Brill, 1112 p. [ Links ]

Farjon, A., Rushforth, K.D., 1989, A classification ofAbiesMiller (Pinaceae): Notes from the Royal Botanical Garden Edinburgh, 46(1), 59-79. [ Links ]

Flores-Mata, G., Jiménez-López, J., Madrigal-Sánchez, X., Moncayo-Ruiz, F., Takaki-Takaki, F., 1971, Memoria del mapa de tipos de vegetación de la República Mexicana: Mexico City, Mexico, Secretaría de Recursos Hidráulicos, Dirección de Agrología, 59 p. [ Links ]

García-Esteban, L., de Palacios-de Palacios, P., Guindeo-Casasús, A., García-Esteban, Ly., Lázaro-Durán, I., González-Fernández, L., Rodríguez-Labrador, Y., Fernández-García, F., Bobadilla-Maldonado, I., Camacho-Ayala, A., 2002, Anatomía e identificación de maderas de coníferas a nivel de especie: Madrid, España, Mundi-Prensa, Fundación Conde del Valle de Salazar, 421 p. [ Links ]

García-Esteban, L. , de Palacios-de Palacios, P. Guindeo-Casasús, A. , García-Fernández F., 2004, Characterisation of the xylem of 352 conifers: Investigación Agraria, Sistemas y Recursos Forestales, 13(3), 452-478. [ Links ]

García-Esteban, L. , de Palacios, P., García-Fernández, F., Moreno, R., 2009, Wood anatomy of the genus Abies a review: IAWA Journal, 30(3), 231-245. [ Links ]

Gómez-Tuena, A., Orozco-Esquivel, M.T., Ferrari, L., 2005, Petrogénesis ígnea de la Faja Volcánica Transmexicana: Boletín de la Sociedad Geológica Mexicana, 57(3), 227-283. [ Links ]

Graham, A., 1976, Studies in neotropical paleobotany II. The Miocene communities of Veracruz, Mexico: Annals of the Missouri Botanical Garden, 63(4), 787-842. [ Links ]

Graham, A., 1999, The Ttertiary history of the northern temperate element in the northern Latin American biota: American Journal of Botany, 86(1), 32-38. [ Links ]

Greguss, P., 1955, Identification of living gymnosperms on the basis of xylotomy: Budapest, Hungary, Akadémiai Kiadó, 720 p. [ Links ]

Huolbert, C., 1910, Les bois des faluns de Touraine, in Lecointre, Les formes diverses de la vie dans les Faluns de Touraine: France, Sixième suite, Feuille Jeunes Naturalistes, 69-76. [ Links ]

Huerta-Vergara, A.R., 2014, Descripción e identificación de impresiones fósiles de coníferas del Cretácico del Norte y Sur de México, con énfasis en la familia Pinaceae: Mexico City, Mexico, Universidad Nacional Autónoma de México, Facultad de Ciencias, BSc Thesis, 100 p. [ Links ]

Köppen, W., 1948, Climatología: Con un estudio de los climas de la Tierra: Mexico City, Mexico, Fondo de Cultura Económica, 479 p. [ Links ]

Lozano-García, S. , 1996, La vegetación del Cuaternario tardío en el Centro de México: Registros Palinológicos e Implicaciones Paleoclimáticas: Boletín de la Sociedad Botánica de México, 58, 113-127. [ Links ]

Lozano-García, M.S., Ortega-Guerrero, B. , 1994. Palynological and magnetic susceptibility records of Lake Chalco, central Mexico: Palaeogeography, Palaeoclimatology, Palaeoecology, 109(1-2), 177-191. [ Links ]

Lozano-García, M.S. , Ortega-Guerrero, B. , 1998. Late Quaternary environmental changes of the central part of the Basin of Mexico; correlation between Texcoco and Chalco basins: Review of Palaeobotany and Palynology, 99(2), 77-93 [ Links ]

Lozano-García, M.S., Ortega-Guerrero, B. , Caballero-Miranda, M., Urrutia-Fucugauchi, J., 1993, Late Pleistocene and Holocene paleoenvironments of Chalco lake, Central Mexico: Quaternary Research, 40(3), 332-342. [ Links ]

Lozano-García, M.S. , Ortega, B., Roy, P.D., Beramendi-Orosco, L., Caballero, M. 2015, Climatic variability in the northern sector of the American tropics since the latest MIS 3: Quaternary Research, 84(2), 262-271. [ Links ]

Lozano-García, S., Brown, T.E., Ortega, B. , Caballero, M. , Werne, J., Fawcett, P.J., Schwalb, A., Valero-Garcés, B., Schnurrenberger, D., O ́Grady, R., Stockhecke, M., Steinman, B., Cabral-Cano, E., Caballero, C., Sosa-Nájera, S. , Soler, A.M., Pérez, L. , Noren, A., Myrbo, A., Bücker, M. , Wattrus, N., Arciniega, A., Wonik, T., Watt, S., Kumar, D., Acosta, C., Martínez, I., Cossio, R., Ferland, T., Vergara-Huerta, F., 2017, Perforación profunda en el lago de Chalco: reporte técnico: Boletin de la Sociedad Geológica Mexicana, 62(2), 299-311. [ Links ]

Martínez-Méndez, N., Aguirre-Planter, E. ,Eguiarte, L.E. , Jaramillo-Correa, J.P. , 2016, Modelado de nicho ecológico de las especies del géneroAbies(Pinaceae) en México: algunas implicaciones taxonómicas y para la conservación: Botanical Sciences, 94(1), 5-24. [ Links ]

Ortega-Guerrero, B. , Thompson, R., Urrutia-Fucugauchi, J. , 2000, Magnetic properties of lake sediments from Lake Chalco, central Mexico, and their palaeoenvironmental implications: Journal of Quaternary Science, 15(2), 127-140. [ Links ]

Ortega-Guerrero, B. , Lozano-García, S. , Herrera-Hernández, D., Caballero, M. , Beramendi-Orosco, L. , Bernal, J.P., Torres-Rodríguez, E., Avedaño-Villeda, D., 2017, Lithostratigraphy and physical properties of the last ca. 150 kyr from Chalco basin, central Mexico: Journal of South American Earth Sciences, 79, 507-524. [ Links ]

Philippe, M., Bamford, M.K., 2009, Houlbert´s types for fossil wood: lectotypification and taxonomic reappraisal ofAbietoxylon,AmbaroxylonandTaxoxylon: Taxon, 58(4), 1349-1356. [ Links ]

Ramírez-Barahona, S., Eguiarte, L.E. , 2013, The role of glacial cycles in promoting genetic diverity in the Neotropics. The case of cloud forests during the Last Glacial Maximum: Ecology and Evolution, 3(3), 725-738. [ Links ]

Roy, S.K., Hills, L.V., 1972, Fossil woods from the Beaufort Formation (Tertiary), northwestern Banks Island, Canada: Canadian Journal of Botany, 50(12), 2637-2648. [ Links ]

Ruiz-Angulo, A., López-Espinoza, E.D., 2015, Estimación de la respuesta térmica de la cuenca lacustre del Valle de México en el siglo XVI: un experimento numérico: Boletín de la Sociedad Geológica Mexicana, 67(2), 215-225. [ Links ]

Rzedowski, J., 2006. Vegetación de México: Mexico City, Mexico, Comisión Nacional para el Conocimiento y Uso de la Biodiversidad, 504 p. [ Links ]

Semerikova, S.A., Semerikov, V.L., 2014, Molecular phylogenetic analysis of the genusAbies(Pinaceae) based on the nucleotide sequence of chloroplast DNA: Russian Journal of Genetics, 50(1), 7-19. [ Links ]

Strandby, U., Christensen, K.I., Sørensen, M., 2009, A morphometric study of theAbies religiosa-hickelii-guatemalensiscomplex (Pinaceae) in Guatemala and Mexico: Plant Systematics and Evolution, 280, 59-76. [ Links ]

Torres-Rodriguez, E., Lozano-García, S. , Roy, P., Ortega, B. , Beramendi-Orosco, L. , Correa-Metrio, A. , Caballero, M. , 2015, Last Glacial droughts and fire regimes in the central Mexican highlands: Journal of Quaternary Science, 30(1), 88-99. [ Links ]

Urrutia-Fucugauchi, J. , Lozano-García, M.S. , Caballero, M. , Ortega-Guerrero, B. , 1995, Paleomagnetic and Paleoenvironmental studies in the southern Basin of Mexico - II: Late Pleistocene-Holocene Chalco lacustrine record: Geofísica Internacional, 34(1), 33-53. [ Links ]

Vázquez-Selem, L., Heine, K., 2011, Late Quaternary Glaciation in Mexico, in Ehlers, J., Gibbard, P.L., Hughes, P.D. (eds.), Quaternary Glaciations - Extent and Chronology, A closer look: Amsterdam, The Netherlands, Elsevier, Developments in Quaternary Science, 15, 849-861. [ Links ]

Watari, S., 1956, Some Abietineen woods from the Tertiary of Japan: Journal of the Faculty of Science, University of Tokyo, Section 3, Botany, 6(9): 419-437. [ Links ]

Xiang, X., Cao, M., Zhou, Z., 2007, Fossil history and modern distribution of the genusAbies(Pinaceae): Frontiers of Forestry in China, 2(4), 355-365. [ Links ]

Xiang, Q.-P., Wei, R., Shao, Y.-Z., Yang, Z.-Y., Wang, X.-Q., Zhang, X.-C., 2015, Phylogenetic relationships, possible ancient hybridization, and biogeographic history of Abies (Pinaceae) based on data from nuclear, plastid, and mitochondrial genomes. Molecular Phylogenetics and Evolution, 82, 1-14. [ Links ]

Received: November 24, 2017; Revised: January 20, 2018; Accepted: January 30, 2018

Creative Commons License This is an open-access article distributed under the terms of the Creative Commons Attribution License