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Agrociencia
versión On-line ISSN 2521-9766versión impresa ISSN 1405-3195
Agrociencia vol.41 no.7 Texcoco oct./nov. 2007
Crop Science
Characterization of onion (Allium cepa L.) varieties based on physical properties and seed performance
1 Genética. Campus Montecillo. Colegio de Postgraduados. Km. 36.5 Carretera México-Texcoco. 56230. Montecillo, Texcoco, Estado de México. (herrera1@colpos.mx) (carrillo@colpos.mx)
Five cultivars (Cristal White Wax, Toro, Red Burgundy, Cojumatlán and Contessa) of onion (Allium cepa L.) were characterized according to: the weight of 100 seeds, germination kinetics, speed germination (T50) and seed protein analysis. Seed of Crystal White Wax was differentiated from the other cultivars by its low weight (0.30 g), since the weight of the rest was 0.40 g. Crystal White Wax had low seed germination (56%) and the highest delay of germination (T50 values were 78 h), while T50 of the rest was 52 h. Protein was isolated from seeds at the precise moment of seed germination. All cultivars were differentiated by their pattern and number of proteins resolved by SDS-PAGE electrophoresis. Toro and Red Burgundy showed the greater difference in the number of proteins detected (27 and 14) whereas Cojumatlán, Contessa and Crystal White Wax had similar numbers. All cultivars shared 12 proteins (44%). According to the coefficient of similarity of Jaccard, Cojumatlán and Crystal White Wax had the greatest degree of relationship (0.95), while Red Burgundy was the least related.
Key words: Allium cepa L.; germination kinetics; SDS-PAGE; T50; vigor
Cinco variedades de cebolla (Allium cepa L.) (Crystal White Wax, Toro, Red Burgundy, Cojumatlán y Contessa) fueron caracterizadas de acuerdo a: peso de 100 semillas, cinética de la germinación, velocidad de germinación (T50) y análisis de proteínas de las semillas. La semilla de Crystal White Wax se diferenció por su menor peso (0.30 g), mientras que el peso del resto de los cultivares fue de 0.40 g. Crystal White Wax tuvo baja germinación (56%) y fue la más tardía (su T50 fue 78 h), mientras que en el resto fue 52 h. La proteína se aisló de las semillas al momento justo de la germinación. Todos los cultivares se diferenciaron por su patrón y número de proteínas detectadas resueltas en geles de poliacrilamida-DSS. Toro y Red Burgundy tuvieron la mayor diferencia en número de proteínas detectadas (27 y 14) mientras que Cojumatlán, Contessa y Cristal White Wax tuvieron números similares. Todos los cultivares compartieron 12 proteínas (44%). De acuerdo con el coeficiente de similitud de Jaccard, Cojumatlán y Crystal White Wax tuvieron el mayor grado de relación (0.95), y Red Burgundy fue el cultivar menos relacionado a los demás.
Palabras clave: Allium cepa L.; cinética de germinación; SDSPAGE; T50; vigor
Acknowledgments
The author wishes to thank to Consejo Nacional de Ciencia y Tecnología (CONACYT), México, for providing a Doctoral fellowship (70459).
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Received: December 2006; Accepted: September 2007
This is an open-access article distributed under the terms of the Creative Commons Attribution License
